Corn production for silage intercropped with forage in the farming-cattle breeding integration

Corn cropping for silage, due to the plant material exportation, intercropping with forage provides greater ground cover and straw formation for the Direct Planting System (DPS) continuity. The objective of this study was to evaluate corn production for silage in DPS intercropped with four forages (Urochloa brizantha cv. Marandu, U. ruziziensis cv. Ruziziensis, Panicum maximum cv. Tanzânia and P. maximum cv. Áries). We applied three sowing methods (in row together with corn fertilizer; by no-till sowing simultaneously to corn sowing and at V4 corn stage) and corn without intercropping. The experiment was conducted in autumn/ winter of 2010, in Selvíria - MS, in a randomized block design in factorial arrangement (4 x 3 + 1) and 4 replications. For corn, we evaluated plant height, basal stem diameter, initial and final stand and silage production and for forage dry matter production. Morphological characteristics and corn yield were not affected by intercropping when compared to sole corn crop. Forage dry matter production sown in corn row with fertilizer is a highlight, which in addition to providing greater productivity, harnesses the operation during sowing.

Studies on chemoenzymatic synthesis: Lipase-catalyzed acylation in multistep organic synthesis

In this thesis, biocatalysis is defined as the science of using enzymes as catalysts in organic synthesis. Environmental aspects and the continuously expanding repertoire of available enzymes have firmly established biocatalysis as a prominent means of chemo-, regio- and stereoselective synthesis. Yet, no single methodology can solve all the challenges faced by a synthetic chemist. Therefore, the knowledge and the skills to combine different synthetic methods are relevant. Lipases are highly useful enzymes in organic synthesis. In this thesis, an effort is being made to form a coherent picture of when and how can lipases be incorporated into nonenzymatic synthesis. This is attempted both in the literature review and in the discussion of the results presented in the original publications contained in the thesis. In addition to lipases, oxynitrilases were also used in the work. The experimental part of the thesis comprises of the results reported in four peer-reviewed publications and one manuscript. Selected amines, amino acids and sugar-derived cyanohydrins or their acylated derivatives were each prepared in enantio- or diastereomerically enriched form. Where applicable, attempts were made to combine the enzymatic reactions to other synthetic steps either by the application of completely separate sequential reactions with isolated intermediates (kinetic and functional kinetic resolution of amines), simultaneously occurring reactions without intermediate isolation (dynamic kinetic resolution of amino acid esters) or sequential reactions but without isolating the intermediates (hydrocyanation of sugar aldehydes with subsequent diastereoresolution). In all cases, lipase-catalyzed acylation was the key step by which stereoselectivity was achieved. Lipase from Burkholderia cepacia was a highly selective enzyme with each substrate category, but careful selection of the acyl donor and the solvent was important as well.

Metabolic evaluation of dairy cows submitted to three different strategies to decrease the effects of negative energy balance in early postpartum

In early lactation dairy cattle suffer metabolic alterations caused by negative energy balance, which predisposes to fatty liver and ketosis. The aim of this study was to evaluate the metabolic condition of high yielding dairy cows subjected to three treatments for preventing severe lipomobilization and ketosis in early lactation. Fifty four multiparous Holstein cows yielding >30 L/day were divided into four groups: control (CN= no treatment), glucose precursor (PG= propylene-glycol), hepatic protector (Mp= Mercepton®), and energy supplement with salts of linolenic and linoleic faty acids (Mg-E= Megalac-E®). Treatments were administrated randomly at moment of calving until 8 weeks postpartum. Blood samples were collected on days 1, 7, 14, 21, 28, 35, 42 and 49 postpartum. Body condition score (BCS) was evaluated at the same periods and milk yield was recorded at 2nd, 4th, 5th, 6th, 7th, and 8th weeks of lactation. Concentrations of non-esterified fatty acids (NEFA), albumin, AST, ß-hydroxybutyrate (BHBA), cholesterol, glucose, total protein, urea and triglycerides were analyzed in blood samples. Cut-off points for subclinical ketosis were defined when BHBA >1.4 mmol/L and NEFA >0.7 mmol/L. General occurrence of subclinical ketosis was 24% during the period. An ascendant curve of cholesterol and glucose was observed from the 1st to the 8th week of lactation, while any tendency was observed with BHBA and NEFA, although differences among treatments were detected (p<0.05). BCS decreased from a mean of 3.85 at 1st week to 2.53 at 8th week of lactation (p=0.001). Milk yield was higher in the Mg-E group compared with the other treatment groups (p<0.05) Compared with the CN group, the treatments with Mp and PG did not show significant differences in blood biochemistry and milk yield. Cows receiving PG and Mg-E showed higher values of BHBA and NEFA (P<0.05), indicating accentuated lipomobilization. Supplementation with Mg-E also resulted in significant higher concentrations of cholesterol, BHBA, urea, AST and lower values of glycemia. This performance may be explained by the highest milk yield observed with this treatment. Treatments with PG and Mp did not improve milk yield, compared with control cows, but did not show metabolic evidence of ketosis, fat mobilization or fatty liver. These results suggest that treatment with Mg-E improves milk production but induces a higher negative energy balance leading to moderated lipomobilization and ketone bodies production, increasing the risk of fatty liver.

Maidon ja heran mikrosuodatus

Työssä selvitettiin uudenaikaisten polymeerikalvojen soveltuvuutta maidon sekä heran mikrosuodatukseen. Kirjallisessa osassa perehdyttiin maidon ja heran koostumuksiin, mikrobikantoihin sekä erilaisiin maidon sekä heran rasvanpoisto- ja puhdistusmenetelmiin. Tämän lisäksi käsiteltiin mikrosuodatusmenetelmiä sekä niiden käyttöä heran ja maidon käsittelyssä meijeriteollisuudessa. Kokeellisessa osassa verrattiin kahdella eri suodatuslaitteistolla keraami- ja polymeerikalvojen suodatusominaisuuksia separoidun raakamaidon sekä heran poikkivirtausmikrosuodatuksessa. Tavoitteena oli erottaa suodatettavista liuoksista mikrobit sekä jäännösrasva ja tuottaa puhdasta, proteiinipitoista maitoa / heraa. Koeajoissa otetuista näytteistä analysoitiin ainesosakoostumus sekä mikrobipitoisuudet. Tuloksista määritettiin retentioarvot ja näiden perusteella vertailtiin polymeeri- ja keraamikalvojen erotustehokkuutta. Lisäksi kalvojen toimivuutta arvioitiin vuoarvojen avulla. Raakamaidon suodatuksissa polymeerikalvot pidättivät täydellisesti tai lähes täydellisesti jäännösrasvan ja mikrobit, mutta myös kaseiiniproteiinin. Tästä syystä yksikään polymeerikalvoista ei sovellu puhtaan maidon suodatukseen. Keraamikalvot soveltuvat tähän selkeästi paremmin ja yksikään toinen testatuista kalvoista ei päässyt samalle tasolle meijeriteollisuudessa jo käytössä olevan Membralox 1,4 μm -keraamikalvon kanssa. Heran suodatuksissa polymeerikalvot pidättivät täydellisesti tai lähes täydellisesti rasvan ja mikrobit, mutta keraamikalvo päästi huomattavasti enemmän haluttuja proteiineja permeaattiin. Membralox 0,8 μm -keraamikalvo pidätti täydellisesti mikrobit, mutta päästi jonkin verran jäännösrasvaa permeaattiin. Näin ollen kalvovalinta on kompromissi permeaatin puhtauden ja proteiinin saannon välillä. Keraamikalvojen vuohäviöt olivat merkittävästi pienemmät kuin polymeerikalvoilla molempien syöttöliuosten kanssa. Lisäksi keraamikalvojen puhdistus oli huomattavasti nopeampaa ja helpompaa. Tästä syystä keraamiset kalvot soveltuvat polymeerisiä paremmin maidon ja heran mikrosuodatukseen.

Weed control via intercropping with gliricidia: II. corn crop

A reduction in herbicide use is one of modern agriculture's main interests and several alternatives are being investigated with this objective, including intercropping. Gliricídia (Gliricidia sepium) mulch has no allelopathic effect on corn or beans but significantly decreased the population of some weed species. The objective of this study was to evaluate green ear and grain yield in corn cultivars as a response to weed control achieved via intercropping with gliricidia. A completely randomized block design with five replicates and split-plots was used. Cultivars AG 1051, AG 2060, BRS 2020, and PL 6880 (assigned to plots) were submitted to the following treatments: no hoeing, hoeing (performed at 20 and 40 days after sowing the corn), and corn intercropped with gliricidia. Gliricidia was grown in a transplanting system to ensure uniform germination and fast establishment in the field. Seeding was made in 200-cell trays with one seed per cell (35 mL volume). The plants emerged two to three days after sowing and were transplanted to a permanent site two to three days after emergence. Corn was sown on the same day gliricidia was transplanted. Sixteen weed species occurred at different frequencies, with uneven distribution in the experimental area. Cultivars AG 1051 and AG 2060 were the best with reference to most characteristics employed to evaluate green corn yield. Cultivar AG 1051 provided the highest grain yield. The highest green ear yield and grain yield values were obtained with hoeing. However, the fact that intercropped plots showed intermediate yield between the values obtained for hoed and non-hoed plots indicates that gliricidia was beneficial to corn, and exerted a certain level of weed control.

Planting density of gliricidia when intercropped with corn for weed control

Reduced use of herbicides that cause environmental pollution problems is of great interest in modern agriculture. Soil mulching with gliricidia (Gliricidia sepium) branches does not have an allelopathic effect on corn, but decreases weed populations. The objective of this study was to evaluate the effects of gliricidia planting density, when grown as an intercrop, on weed control and corn yield parameters. A randomized block design with split-plots and ten replicates was adopted. Corn cultivars AG 1051 and BM 3061 were grown without hoeing, with two hoes (at 24 and 44 days after planting), and intercropped with gliricidia (planted simultaneously with corn, between crop rows, using two seedlings/pit, spaced at 30, 40, or 50 cm). Twenty-one weed species were found in the experimental area. Increased gliricidia planting density reduced weed biomass, but no difference was found between weed biomass in the intercrop and weed biomass in non-hoed corn. Gliricidia intercropped with corn, planted at a row spacing of 30 cm, did not significantly differ from hoed corn in most characteristics considered to evaluate green corn yield, although mean values were smaller. As to the number and weight of marketable green ears, reductions of 5% and 13%, respectively, were observed. Intercropping caused a 17% reduction in grain yield, reducing the losses (36%) observed in non-hoed corn by more than 50%. The highest green ear yield and grain yield values were obtained with two hoeings, while the lowest values were observed for non-hoed corn. The cultivars did not differ regarding green ear yield and grain yield.

Effects of weed "Reestablishment" after hoeing on corn yields

Some growers and researchers sustain the idea that regrowth or root setting of some weeds may occur after hoeing, with detrimental effects over corn. The objective of this study was to evaluate the effects of weed removal from the field, removal after each hoeing, and corn intercropped with gliricidia on weed control and corn yield values. The experimental design consisted of blocks with split-plots and six replicates. Cultivars AG 1051 and BM 2022, planted in the plots, were submitted to the following treatments: no hoeing, two hoeings (at 20 and 40 days after planting), and intercropped with gliricidia. The hoed plots were either submitted to weed removal after the first, second, or both hoeings, or remained without weed removal. In the intercropped treatment, gliricidia was sown by broadcasting at corn planting between the corn rows, at a density of 15 seeds m-2. Twenty-five weed species occurred in the experiment; the most frequent was Digitaria sanguinalis (family Poaceae). The weed control methods tested had similar effects on the cultivars, which were not different from one another with respect to the evaluated traits, except for one-hundred-kernel weight, with cultivar AG 1051 being superior. Weed removal did not influence green corn yield or grain yield. However, the number of kernels/ear was higher in plots where weeds were removed in relation to plots without weed removal, suggesting that weed removal might be beneficial to corn. Besides, a higher dry matter weight was obtained for the above-ground part of weeds removed from the field after the first and second hoeings than the weight of weeds removed after the second hoeing only which, in turn, was higher than the weight of weeds removed after the first hoeing only. Green ear yield, grain yield, and dry matter of the above-ground part of the weeds did not show differences in hoed plots and were superior to the non-weeded plots and the intercropped plots, which were not different from each other; therefore, intercropping with gliricidia did not improve corn yield values.

Globe fringerush (Fimbristylis miliacea) cross resistance to als-inhibitor herbicides under field conditions in irrigated rice in the south of Brazil

ALS-inhibiting herbicides usually provide adequate weed control in irrigated rice fields. After consecutive years of use, the Cyperaceae species, globe fringerush (Fimbristylis miliacea) began to show resistance to ALS (acetolactate synthase) inhibitors. Globe fringerush is one of the most problematic herbicide-resistant weeds in irrigated rice in the state of Santa Catarina in the South of Brazil. The objective of this research was to examine cross resistance of globe fringerush to ALS inhibitors, under field conditions. Two experiments were conducted in a rice field naturally infested with ALS-resistant globe fringerush in Santa Catarina, in the 2008/09 and 2009/10 cropping seasons. The experimental units were arranged in randomized complete block design, with five replicates, consisting of two factors (herbicide and dose) in a 4 x 5 factorial arrangement. ALS herbicides included bispyribac-sodium, ethoxysulfuron, pyrazosulfuron-ethyl and penoxsulam. Six-leaf globe fringerush was sprayed with herbicide doses of 0, 0.5, 1, 2 and 4X the recommended doses in a spray volume of 200 L ha-1. The number of rice culm, filled and sterile grains, plant height, dry shoot biomass and grain yield were recorded. Globe fringerush control was evaluated 28 and 70 days after herbicide application (DAA); shoots were harvested at 13 weeks after herbicide application and dry weight recorded. Competition with globe fringerush reduced the number of culm and rice grain yield. The globe fringerush biotype in this field was resistant to all ALS herbicides tested. Penoxsulam had the highest level of activity among treatments at 28 and 70 DAA, but the control level was only 50% and 42%, respectively, in the second year of assessment. This was not enough to prevent rice yield loss. Alternative herbicides and weed control strategies are necessary to avoid yield losses in rice fields infested with ALS-resistant biotypes of globe fringerush.

Screening of resistance to egyptian broomrape infection in tomato varieties

Parasitic weed species of the genus Orobanche are serious threat for the production of several crops in Europe, Africa and Asia. Research on resistant host plant varieties is one of the most effective management strategies for this parasitic weed. In this study, the susceptibility of twenty-nine tomato varieties to broomrape infection (Orobanche aegyptiaca) under greenhouse conditions was investigated. The employed experimental design was completely randomized with three replications. Differences in susceptibility to infection were monitored among tomato varieties based on their difference in the number of emerged shoots of broomrape and broomrape dry weight (shoots and tubercles). Date of Orobanche emergence varied over a period of 3 to 30 days between varieties. Very late infection was monitored for varieties of Cal-jN3, Viva, Caligen 86, Packmor, CSX 5013, Hyb. PS 6515 and Hyb Petopride5. Differences in the growth and fruit yield among tomato varieties were also found in response to broomrape infestation. Moderate levels of resistance were obtained in Viva, Caligen 86, Hyb. PS 6515, Hyb.Firenze (PS 8094) and Cal-jN3 among other tomato varieties. In contrast, varieties of Kimia-Falat, Hyb. Petopride II and Hyb.AP865 were the most susceptible hosts to Orobanche aegyptiaca.

Wild onion (Asphodelus tenuifolius) competition in rainfed chickpea-chickpea cropping system

Chickpea yield potential is limited by weed competition in typical chickpea growing areas of Pakistan where zero tillage crop grown on moisture conserved from rains received during the months of September and August. The objective of this work was to evaluate the growth and yield characteristics of chickpea grown in coexistence with increasing densities of wild onion (Asphodelus tenuifolius). The experiment was comprised of six density levels viz. zero, 20, 40, 80, 160 and 320 plants m-2 of A. tenuifolius. A decrease in chickpea primary and secondary branches per plant, pods per plant, seeds per pod, 100-seed weight and seed yield was observed due to more accumulation of dry matter per increasing densities of A. tenuifolius. The increase in A. tenuifolius density accelerated chickpea yield losses and reached the maximum values of 28, 35, 42, 50, 58 and 96% at 20, 40, 80, 160 and 320 A. tenuifolius plants m-2, respectively. The yield loss estimation model showed that chickpea losses with infinite A. tenuifolius density were 60%. Yield reduction could be predicted by 2.52% with increase of one A. tenuifolius plant m-2. It is concluded that A. tenuifolius has a strong influence on chickpea seed yield and showed a linear response at the range of densities studied.

Volunteer RR® corn management in roundup ready® soybean-corn succession system

The present study evaluated the effects of cover crops (Pennisetum glaucum, Crotalaria spectabilis and Urochloa ruziziensis) associated with the application of herbicides {glyphosate; (glyphosate + haloxyfop-R); (glyphosate + fluazifop-p-butyl); (glyphosate + imazethapyr) and (glyphosate + imazaquin)} in soybean desiccation management for volunteer RR® corn control. The experiment was conducted under field conditions at Sinop-MT, during the 2013/2014 crop season, in a randomized complete blocks design with factorial scheme and four replications. The following parameter were evaluated: dry matter of cover crops and ground coverage rate, control of volunteer RR® corn present at the time of desiccation, dry matter, height and intoxication level on soybean plants caused by herbicides at 7, 14 and 28 days after emergence (DAE), control of volunteer RR® corn derived from emergence fluxes subsequent to desiccation management and soybean yield. The joint application of (glyphosate + haloxyfop-R) provided the best level of volunteer RR® corn control present at the time of desiccation. Satisfactory control (80%) of volunteer corn was obtained with the application of (glyphosate + imazethapyr). This treatment displayed an additional residual effect of imazethapyr, which efficiently controled volunteer RR® corn derived from fluxes subsequent to desiccation management, especially in treatments performed under U. ruziziensis straw. None of the herbicides used in desiccation management caused any significant effect on dry matter, height and phytotoxicity of soybean plants at 7, 14 and 28 DAE nor on grain yield.

Assessing the Competitive Ability of Rhynchosia capitata; an Emerging Summer Weed in Asia

Rhynchosia capitata is a newly emerging threatening weed of summer crops in many Asian countries. We conducted 2-yr experiments to evaluate R. capitata competition with mungbean under irrigated conditions. Rhynchosia capitata was allowed to compete with mungbean for 0, 3, 4, 5, 6, 7 weeks after planting and for full crop season. The competitive ability of R. capitata was assessed by measuring its dry weight, micro and macro nutrient contents and uptake; and its effects on mungbean growth and yield parameters. The results showed that full season weed competition produced highest dry weight of R. capitata and its macro and micronutrient contents and uptake. Yield and yield components of mungbean decreased in a linear fashion with increase in competition period of Rhynchosia capitata. Full season competition of R. capitata reduced the seed yield of mungbean by 49% and 46% during 2011 and 2012 respectively. In conclusion, damaging effect of R. capitata intrusion on mungbean yield is associated with duration of its presence in crop, accumulation of dry matter and the nutrient uptake by R. capitata, which otherwise should be available to crop.

Weed Control in Maize with Gliricidia Intercropping

One of the very important components in the organic maize production costs refers to spending on weed control. In this research were assessed the effects of maize hybrids (AG 1051 and BR 205) in an intercropping with Gliricidia (Gliricidia sepium) and mechanical hoeing on weed control. The treatments assessed were: A - maize monocropping + two hoeings (20 and 40 days after sowing); B - maize with one hoeing at 20 days + intercropping with gliricidia sowed after hoeing; C - maize sowing intercropped with gliricidia at the time of maize sowing + hoeing at 40 days; D - maize sowing intercropped with gliricidia at the time of maize sowing without hoeing; E - monocropping maize without hoeing. In the intercroppings, gliricidia was sowed in broadcast seeding with 30 viable seeds m-2. Maize hybrids did not differ in their effects on weed growth and grain yield. Treatments A, B and C have reduced weed growth, compared to treatments D and E. The highest grain yield was obtained with treatment A and the lowest with treatment E. It was concluded that intercropping maize and gliricidia is not a good alternative for an integrated weed management in maize crops in the conditions assessed.

APPLICATION HEIGHT IN HERBICIDES EFFICIENCY IN BEAN CROPS

ABSTRACTInadequate herbicide application can result in failures in weed control and/or poisoning of the crops, resulting in yield losses. In this research were assessed the effects of the sprayer nozzle boom height in the distribution of the spray solution for weed control, influencing intoxication of beans and crop yield. Experiments were conducted in laboratory and field conditions. In laboratory, the performance of flat spray tip TT 11002 was assessed at heights 0.20, 0.30, 0.40 and 0.50 meters with respect to the target surface. In the field the same heights were assessed in applications of herbicides fomesafen, fluazifop-P-butyl and fomesafen + fluazifop-P-butyl. There was an inverse relationship between the height of the spray boom and the coefficients of variation of the patterns. The mixture better efficiency in a tank of fluazifop-P-butyl + fomesafen was obtained with the height of 0.50 m from the target. This treatment resulted in better weed control, lower poisoning of the bean plants and better crop yield rates.

INTERCROPPING CORN WITH A COMBINATION OF TREE SPECIES TO CONTROL WEEDS

ABSTRACT The combination of crop residues or crop extracts is often more advantageous in controlling weeds, than the application of each residue or extract singly. This suggests that in intercropping with maize, the combination of tree species can be more advantageous than species isolated in weed control. The objective of this study was to evaluate the effects of intercropping with a combination of leguminous on the weed growth and corn yield. A randomized-block design with split plots (cultivars in plots) and five replicates was established. The cultivars BR 205 and AG 1041 were subject to the following treatments: two weedings (A), intercropping with sabiá (B), gliricidia (C), gliricidia + sabiá (D) and no weeding (E). In the B and C, 30 viable seeds m-2 of the leguminous were sown. In the D, 15 seeds of each species were sown m-2. The legumes were sown by random casting during corn planting. The sequence of the best treatments in reducing the growth of weeds is A > B = C = D = E. The sequence of the best treatments when are considered the yields of baby corn, green corn and grain is A > B > C > D > E. The cultivars do not differ in regards to the reduction in weed growth. In terms of corn yield cultivar BR 205 is the best.