Women's contribution to equality in Latin America and the Caribbean

Includes bibliography

Millennium development goals: advances in environmentally sustainable development in Latin America and the Caribbean

Includes bibliography

Porphyromonas gingivalis LPS stimulation downregulates DNMT1, DNMT3a, and JMJD3 gene expression levels in human HaCaT keratinocytes

Objective: The role of epigenetic regulation in inflammatory diseases such as periodontitis is poorly known. The aim of this study was to assess whether Porphyromonas gingivalis lipopolysaccharide (LPS) can modulate gene expression levels of the some enzymes that promote epigenetic events in cultures of the human keratinocytes and gingival fibroblasts. In addition, the same enzymes were evaluated in gingival samples from healthy and periodontitis-affected individuals. Materials and methods: Primary gingival fibroblast and keratinocyte (HaCaT) cultures were treated with medium containing P. gingivalis LPS or P. gingivalis LPS vehicle for 24 h. After this period, cell viability was assessed by MTT test and total RNA extracted to evaluate gene expression levels of the following enzymes by qRT-PCR: DNA methyltransferase 1 (DNMT1), DNA methyltransferase 3a (DNMT3a), histone demethylases Jumonji domain containing 3 (JMJD3) and ubiquitously transcribed tetratricopeptide repeat, X chromosome (UTX). To evaluate gene expression in healthy and periodontitis-affected individuals, total RNA was extracted from biopsies of gingival tissue from healthy and periodontitis sites, and gene expression of DNMT1, DNAMT3a, JMJD3, and UTX was evaluated by qRT-PCR. Results: No significant differences were found in the gene expression analysis between healthy and periodontitis-affected gingival samples. The results showed that LPS downregulated DNMT1 (p < 0. 05), DNMT3a (p < 0. 05), and JMJD3 (p < 0. 01) gene expression in HaCaT cells, but no modulation was observed in gingival fibroblasts. Conclusion: P. gingivalis LPS exposure to human HaCaT keratinocytes downregulates gene expression of the enzymes that promote epigenetic events. Clinical relevance: The advance knowledge about epigenetic modifications caused by periodontopathogens may to possibly led to the development of new periodontal therapies. © 2012 Springer-Verlag.

Aníbal Pinto Santa Cruz

Aníbal Pinto Santa Cruz, Director of the Review since 1986, died on 3 January. His death fills us with profound grief and leaves a deep vacuum in this organization. The Economic Commission for Latin America and the Caribbean benefited for many years from the intellectual sparkle and human warmth of Mr. Pinto, who served for several years as Director of the Economic Development Division. What is more, he was one of the personalities who gave the ECLAC secretariat a clear institutional identity. The depth and clarity of his analyses of Chile and its development process were matched by his real dedication to Latin America, which inspired him to make solid and valuable contributions to the progress of ideas in our region. He belonged in his own right to the select group of those thinkers whose new categories and concepts afford others a richer vision of reality. It is not surprising, therefore, that followers and former students of his abound in the region. A person of great intellectual generosity, impatient with conventional wisdom and intolerance from all academic and political quarters, Aníbal Pinto received recognition from the international academic community, as embodied in the Raúl Prebisch Ibero­American Prize in Economics, an honorary doctorate from the University of Campinas, Brazil, and the Chilean National Prize in the Humanities and Social Sciences for 1995. In recent months he received two further distinctions: first, a tribute from his ECLAC colleagues on the occasion of the fiftieth anniversary of the United Nations, and second, a collection of his writings published by the Universidad Nacional Autónoma de México which was presented to him in a ceremony held at the Santiago Book Fair in December 1995. ECLAC has been immensely fortunate in having among its senior officials great personalities who have left behind a legacy of values, principles and key ideas; institution­builders, if you will. If there is anything which distinguishes ECLAC from other United Nations bodies, it is this. Aníbal Pinto's name will undoubtedly be among those which resound the loudest. For this reason, and for his exceptional human qualities, we shall remember him with affection and admiration.

Economic growth and human development in Latin America

Includes bibliography

Absence of mutagenicity of plants used to treat gastrointestinal disorders

The Brazilian Savanna (locally called Cerrado) is an important biome presenting several plants that are used in popular medicine. However, the risks associated with the consumption of derivatives from these plants are generally unknown. Studies with compounds obtained from different species have shown the risks of DNA damage. The present work assessed the in vivo mutagenicity of three plant species used in popular medicine to treat human gastrointestinal disorders (Mouriri pusa, Qualea grandiflora and Qualea multiflora). The micronucleus assay was performed in peripheral blood of mice submitted to acute treatments. Results showed that no assessed extracts were mutagenic in vivo. In fact, the absence of mutagenicity in the present study indicates that the extracts do not contain compounds capable of inducing DNA breaks or chromosomal loss. However, further analysis should be performed in others systems to guarantee their safety, mainly to human chronic use.

Doppler echocardiography in athletes from different sports

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Toxicogenetic monitoring in urban cities exposed to different airborne contaminants

Microparticles found in the air may be associated with organic matter that contains several compounds, such as Polycyclic Aromatic Hydrocarbons (PAHs) and nitro-PAHs, and may pose a significant risk to human health, possibly leading to DNA mutations and cancers. This study associated genotoxicity assays for evaluating human exposure with the atmospheric air of two urban areas in southern Brazil, that received different atmospheric contributions. Site 1 was under urban-industrial influence and the other was a non-industrial reference, Site 2. Organic extracts from the airborne particulate matter were tested for mutagenicity via the Salmonella/microsome assay and analyzed for PAH composition. Cells samples of people residing in these two cities were evaluated using the comet and micronucleus assay (MN).Concentrations of the individual PAHs ranged from 0.01ng/m3 (benzo[a]anthracene) to 5.08ng/m3 (benzo[ghi]perylene). As to mutagenicity analysis of airborne, Site 1 presented all the mutagenic responses, which varied from 3.2±1.22rev/m3 (TA98 no S9) to 32.6±2.05rev/m3 (TA98, S9), while Site 2 ranged from negative to minimal responses. Site 1 presented a high quantity of nitro and amino derivatives of PAHs, and peaked at 56.0±3.68rev/μg (YG1024 strain). The two groups presented very low DNA damage levels without intergroup difference. Although Site 1 presented high mutagenic responses in the air samples, high PAH levels, healthy people exposed to this environment did not show significative damage in their genetic material. However, the evaluation of different environmental and genetic damage in such population is necessary to monitor possible damages. © 2013 Elsevier Inc.

A saúde e seu direito no olhar do usuário sob a luz da constituição de 1988

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise do processo de reparo do reimplante de incisivos de ratos diabéticos após a manutenção dos dentes em leite

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A responsabilidade do Estado por dano causado à imagem

Pós-graduação em Direito - FCHS

Responsabilidade do Estado por atos das forças policiais

Pós-graduação em Direito - FCHS

Positivismo e direito: a semântica construtiva

Pós-graduação em Filosofia - FFC

A justiciabilidade do direito à qualidade do ensino fundamental no Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A mobilidade urbana em questão: um olhar sobre a cidade de Viçosa - MG

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)