951 resultados para RNA interference (RNAi)
Resumo:
A type of thermo-optic variable optical attenuator based on multimode interference coupler is proposed. The optical field propagation properties of the devices are simulated using finite difference beam propagation method. The propagation loss of the fabricated device is 2-4.2 dB at the wavelength range 1510-1610 nm. The total power consumption is 370 mW and the maximum attenuation is more than 25 dB, which almost can meet the requirements of optical fiber communication systems.
Resumo:
Fourth-order spatial interference of entangled photon pairs generated in the process of spontaneous parametric down-conversion pumped by a femtosecond pulse laser has been performed for the first time. In theory, it takes into account the transverse correlation between the two photons and is used to calculate the dependence of the visibility of the interference pattern obtained in Young's double-slit experiment. In this experiment, a short focal length tens and two narrow band interference filters were adopted to eliminate the effects of the broadband pump laser and improve the visibility of the interference pattern under the condition of nearly collinear light and degenerate phase matching.
Resumo:
We have used the transverse correlated properties of the entangled photon pairs generated in the process of spontaneous parametric down-conversion, which is pumped by a femtosecond pulse laser, to perform Young's interference experiment. Unlike the case of a continuous wave laser pump, a broadband pulse laser pump can submerge an interference pattern. In order to obtain a high visibility interference pattern, we used a lens with a tunable focal length and two interference filters to eliminate the effects of the broadband pump laser. It is proven that the process of two-photon direct interference is a post-selection process.
Resumo:
We have fabricated a compact 3-dB multimode interference coupler with a large silicon-on-insulator cross section. To reduce the length of the usual symmetric interference multimode interference coupler, we propose using a parabolically tapered structure. The length of the device is 398 mum. The device has a uniformity of 0.28 dB. (C) 2001 Optical Society of America.
Resumo:
Analytical expression of signal bandwidth of general straight and tapered N x N multimode interference (MMI) couplers is presented. The signal bandwidth is characterized as a function of mode relative energy, mode propagation delay time, and mode pulse broadening in the multimode section of MMI coupler. The model is used to evaluate the signal bandwidth of specific couplers. Results indicate that the signal bandwidth decreases seriously with the increase of channel number and channel guide space. Compared with the straight MMI coupler, the tapered MMI coupler has an improved signal bandwidth.
Resumo:
We demonstrate a type of 2 x 2 multimode interference 3 dB coupler based on silicon-on-insulator. The fabrication tolerance was investigated by the effective index method and the guide mode method. The devices with different lengths were fabricated and near-held output images were obtained. Tolerances to width, length and etch depth are 2, 200 and 2 mum, respectively. The devices show a uniform power distribution.
Resumo:
The authors demonstrate a 3dB 2 x 2 parabolically tapered multimode interference (MMT) coupler with a large cross-section and space between the different ports using silicon-on-insulator technology. The device exhibits a uniformity of < 0.8dB and can be used in the realisation of an MMI-based optical switch with a high extinction ratio.
Interference effects in differential reflectance spectra of the GaAs epilayers grown on Si substrate
Resumo:
We report the observation of oscillating features in differential reflectance spectra from the GaAs epilayer grown on Si substrate in the energy range both below and above the fundamental band gap. It is demonstrated that the oscillating features are due to the difference in the interference between two neighboring areas of the sample. The interference arises from two light beams reflected from different interfaces of the sample. The calculated spectra in the nonabsorption region are in good agreement with measured data. It is shown that the interference effect can be used as a sensitive method to characterize the inhomogeneity of the semiconductor heterostructures. (C) 1998 American Institute of Physics. [S0021-8979(98)08723-4].
Resumo:
During the packaging of optoelectrome device, a problem always met is the instability of output power. The main effect causing this problem, Fabry-Perot interference, is discussed in this paper. Both theoretical analysis and experimental test are carried out and in good agreement. As an example of avoiding the disadvantage of Fabry-Perot interference, the packaging process of Silicon-on-Insulator (SOI) based Variable Optical Attenuator(VOA) is shown at last.
Resumo:
A pure surface plasmon polariton (SPP) model predicted that the SPP excitation in a slit-groove structure at metallodielectric interfaces exhibits an intricate dependence on the groove width P. Lalanne et al. [Phys. Rev. Lett. 95, 263902 (2005); Nat. Phys. 2, 551 (2006)]. In this paper, we present a simple far-field experiment to test and validate this interesting theoretical prediction. The measurement results clearly demonstrate the predicted functional dependence of the SPP coupling efficiency on groove width, in good agreement with the SPP picture.
Resumo:
A method for producing optical structures using rotationally symmetric pyramids is proposed. Two-dimensional structures can be achieved using acute prisms. They form by multi-beam interference of plane waves that impinge from directions distributed symmetrically around the axis of rotational symmetry. Flat-topped pyramids provide an additional beam along the axis thus generating three-dimensional structures. Experimental results are consistent with the results of numerical simulations. The advantages of the method are simplicity of operation, low cost, ease of integration, good stability, and high transmittance. Possible applications are the fabrication of photonic micro-structures such as photonic crystals or array waveguides as well as multi-beam optical tweezers. (c) 2006 Optical Society of America.
Resumo:
The mechanism of beam splitting and principle of wide-field-of-view compensation of modified Savart polariscope in the wide-field-of-view polarization interference imaging spectrometer (WPIIS) are analyzed and discussed. Formulas for the lateral displacement and optical path difference (OPD) produced by the modified Savart polariscope are derived by ray-tracing method. The theoretical and practical guidance is thereby provided for the study, design, modulation, experiment and engineering of the polarization interference imaging spectrometers and other birefringent Fourier-transform spectrometers based on Savart polariscopes. (c) 2006 Elsevier B.V. All rights reserved.
Resumo:
The influence of the orientations of both polarizer and analyzer on modulation depth of spatially distributed interferograms for static polarization interference imaging spectrometer (SPIIS) is analyzed. A generally, theoretical relationship to determine the modulation depth of a SPIIS is derived. The special cases of maximum modulation depth (V = 1) and the minimum modulation depth (V = 0) are examined. Our results will provide a theoretical and practical guide for studying, developing and engineering polarization interference imaging spectrometers. (C) 2003 Elsevier B.V. All rights reserved.
Resumo:
新外显子的起源是一种重要的增加转录组和蛋白质组多样性的分子机制。 对于新外显子及其父本基因的进化和功能特征方面还有很多重要的问题有待于 解决。本研究首先在全基因组水平上鉴定在人和小鼠中产生的新外显子,随后 对这些外显子及其父本基因作进化和功能上的分析。我们发现新外显子倾向于 位于基因的UTR 区域,尤其是5’ UTR 区域,这表明可能有些新外显子的出现 与基因的表达调控相关。我们还发现,产生新外显子的基因具有较高的组织表 达特异性,其基因功能倾向于细胞调控和与外界环境相互作用。通过对外群中 直系同源基因的分析,我们的结果表明进化速率较高的基因更容易获得新的外 显子,纠正了先前认为的获得新外显子会加速基因进化速率的看法。 我们对哺乳类CDYL 基因家族中产生的新外显子进行了具体的进化分析和 功能研究。我们的结果表明CDYL 基因在哺乳类分化前在原先的基因上游区域 获得了一个新的启动子和三个新的外显子。随后在哺乳动物各个支系的分化中, CDYL 基因在小鼠,狗和人中分别独立的进化出一个新的外显子。同源比对的 结果表明,这些新外显子是通过内含子序列的外显子化这一分子机制产生。近 缘物种间的进化速率的计算结果表明这些新产生的外显子具有快速进化的模 式,并且其快速进化可能是由正选择所驱动。在人中,多种突变包括新外显子 的获得,启动子的改变,选择性剪切的发生使得人的CDYL 基因获得了一种新 的编码更长蛋白质的剪切体。在人Hela 细胞系中的实验表明,新产生的蛋白质 与原有的蛋白质相比都具有显著的转录抑制活性,但新的蛋白质的转录抑制活 性较弱,且两者之间存在相互干扰的关系。这一结果表明通过新外显子的获得 产生的新的蛋白质可以丰富原有的基因表达调控体系,使得生物体的调控网络 更加精确。 嵌合RNA 通常认为是由来源于不同的pre-mRNA 的外显子通过反式剪切连 接在一起形成的。这一现象在包括多种动物和植物中被广泛的报道。我们的研 究首先通过大规模表达序列(ESTs)的搜索,在酵母,果蝇,小鼠和人中鉴定 到了大量的嵌合RNA。这一结果表明形成嵌合RNA 在真核生物中是一种普遍 的生物学过程,是一种重要的增加转录组和蛋白质组的多样性的分子机制。对 嵌合RNA 的序列分析表明,仅有<20%的嵌合RNA 在接合处可以找到典型的剪切位点 GU-AG,可以用经典的反式剪切模型来解释其产生机制。然而有意思的 是,我们在大约一半的嵌合RNA 的供体基因之间找到了短的同源序列,这一发 现使我们提出了一种新的分子机制来解释这些嵌合RNA 的形成,我们称之为 “转录滑动”模型。在酵母我们,我们用实验的方法验证了短同源序列对形成嵌 合RNA 的必要性,有力地支持了我们这一模型。