910 resultados para Polyoxometalate Cage


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Periazetabuläre Frakturen bei Hüftprothesen nehmen aufgrund der Überalterung und der zunehmenden Aktivität alter Menschen zu. Die periprothetischen Azetabulumfrakturen werden anhand der Einteilung von Letournel klassifiziert. Wenn beide Azetabulumpfeiler bei Hüftprothese betroffen sind, wird auch von einer Beckendiskontinuität gesprochen. Durch eine laterale Kompression können auch periazetabuläre Schambeinastfrakturen und/oder transiliakale Frakturen auftreten. Für die Therapieentscheidung (konservativ, alleinige Osteosynthese, Revisionshüfttotalprothese mit oder ohne zusätzliche Osteosynthese des Vorder- und/oder Hinterpfeilers) und die Zugangswahl bei operativer Versorgung werden patientenspezifische (Alter, Morbidität, Osteoporose, Aktivitätslevel des Patienten), frakturspezifische (Frakturtyp, Dislokationsausmaß, Impression des Doms oder der Hinterwand) und auch prothesenspezifische Faktoren (Art der implantierten Prothese [Hemiprothese vs. Totalprothese], Pfannenstabilität, Zeichen eines Prothesenabriebs, Ausmaß und Lokalisation einer azetabulären Lyse, Stabilität und Lysezeichen des Prothesenschafts) berücksichtigt. Bei akuten Beckendiskontinuitäten werden neben einer Osteosynthese des dorsalen Pfeilers zunehmend eine schnell ossär integrierbare Pfanne (Tantalum [„Trabecular Metal“: TM]) mit oder ohne Augment und/oder Allograft und allenfalls in einer sog. „Cup-Cage“-Technik (TM-Pfanne mit einem abstützenden Revisionsring [Burch-Schneider-Ring] analog zur Therapie von chronischen Beckendiskontinuitäten empfohlen. Bei großen Lysezonen und starken Dislokationen des vorderen Pfeilers und der quadrilateralen Fläche können intrapelvine Zugänge (modifizierter Stoppa- oder Pararectus-Zugang nach Keel) zur zusätzlichen Zuggurtungsosteosynthese des vorderen Pfeilers und Abstützung der quadrilateralen Fläche gewählt werden.

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Keel bone damage (KBD) is a critical issue facing the laying hen industry today as a result of the likely pain leading to compromised welfare and the potential for reduced productivity. Recent reports suggest that damage, while highly variable and likely dependent on a host of factors, extends to all systems (including battery cages, furnished cages, and non-cage systems), genetic lines, and management styles. Despite the extent of the problem, the research community remains uncertain as to the causes and influencing factors of KBD. Although progress has been made investigating these factors, the overall effort is hindered by several issues related to the assessment of KBD, including quality and variation in the methods used between research groups. These issues prevent effective comparison of studies, as well as difficulties in identifying the presence of damage leading to poor accuracy and reliability. The current manuscript seeks to resolve these issues by offering precise definitions for types of KBD, reviewing methods for assessment, and providing recommendations that can improve the accuracy and reliability of those assessments.

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Keel bone damage (KBD) is a critical issue facing the contemporary laying hen industry due to the likely pain leading to compromised welfare and reduced productivity. Recent reports suggest that KBD, while highly variable and likely dependent on a host of factors, extends to all housing systems (including traditional battery cages, furnished cages and non-cage systems), genetic lines, and management styles. Despite the extent of the problem, the research community remains uncertain as to the causes and influencing factors of KBD. To combat these issues, the current review was produced following discussions from the 1st International Keel Bone Damage Workshop held in Switzerland in April 2014. This exercise sought to assess current knowledge, foster novel collaborations, propose unique methodologies and highlight the key areas where innovative research is needed. The following paper is based on the content of those discussions and presents nine recommendations for future research efforts.

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Behavioural tests to assess affective states are widely used in human research and have recently been extended to animals. These tests assume that affective state influences cognitive processing, and that animals in a negative affective state interpret ambiguous information as expecting a negative outcome (displaying a negative cognitive bias). Most of these tests however, require long discrimination training. The aim of the study was to validate an exploration based cognitive bias test, using two different handling methods, as previous studies have shown that standard tail handling of mice increases physiological and behavioural measures of anxiety compared to cupped handling. Therefore, we hypothesised that tail handled mice would display a negative cognitive bias. We handled 28 female CD-1 mice for 16 weeks using either tail handling or cupped handling. The mice were then trained in an eight arm radial maze, where two adjacent arms predicted a positive outcome (darkness and food), while the two opposite arms predicted a negative outcome (no food, white noise and light). After six days of training, the mice were also given access to the four previously unavailable intermediate ambiguous arms of the radial maze and tested for cognitive bias. We were unable to validate this test, as mice from both handling groups displayed a similar pattern of exploration. Furthermore, we examined whether maze exploration is affected by the expression of stereotypic behaviour in the home cage. Mice with higher levels of stereotypic behaviour spent more time in positive arms and avoided ambiguous arms, displaying a negative cognitive bias. While this test needs further validation, our results indicate that it may allow the assessment of affective state in mice with minimal training— a major confound in current cognitive bias paradigms.

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Non-cage housing systems for laying hens such as aviaries provide greater freedom to perform species-specific behavior and thus are thought to improve welfare of the birds; however, aviaries are associated with a high prevalence of keel bone damage (fractures and deviations), which is a major welfare problem in commercial laying hens. Potential causes of keel bone damage are falls and collisions with internal housing structures that occur as birds move between tiers or perches in the aviary. The aim of this study was to investigate the scope for reducing keel bone damage by reducing falls and collisions through modifications of aviary design. Birds were kept in 20 pens in a laying hen house (225 hens per pen) that were assigned to four different treatments (n = 5 pens per treatment group) including (1) control pens and pens modified by the addition of (2) perches, (3) platforms and (4) ramps. Video recordings at 19, 22, 29, 36 and 43 weeks of age were used to analyze controlled movements and falls (including details on occurrence of collision, cause of fall, height of fall and behavior after fall) during the transitional dusk and subsequent dark phase. Palpation assessments (focusing on fractures and deviations) using 20 focal hens per pen were conducted at 18, 20, 23, 30, 37, 44, 52 and 60 weeks of age. In comparison to the control group, we found 44% more controlled movements in the ramp (P = 0.003) and 47% more controlled movements in the platform treatments (P = 0.014) as well as 45% fewer falls (P = 0.006) and 59% fewer collisions (P < 0.001) in the ramp treatment. There were no significant differences between the control and perch treatments. Also, at 60 weeks of age, 23% fewer fractured keel bones were found in the ramp compared with the control treatment (P = 0.0053). After slaughter at 66 weeks of age, no difference in keel bone damage was found between treatment groups and the prevalence of fractures increased to an average of 86%. As a potential mechanism to explain the differences in locomotion, we suggest that ramps facilitated movement in the vertical plane by providing a continuous path between the tiers and thus supported more natural behavior (i.e. walking and running) of the birds. As a consequence of reducing events that potentially damage keel bones, the installation of ramps may have reduced the prevalence of keel fractures for a major portion of the flock cycle. We conclude that aviary design and installation of specific internal housing structures (i.e. ramps and platforms) have considerable potential to reduce keel bone damage of laying hens in aviary systems.

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Human a2 -macroglobulin ( a2 M; homotetramer, Mr 720 kDa) is an essential scavenger of proteinases in the serum. Each of its four subunits has a ‘bait region’, with cleavage sequences for almost all endo-proteinases, an unusual thiol ester moiety and a receptor-binding domain (RBD). Bait region cleavage in native a2 M ( a2 M-N) by a proteinase results in rapid thiol ester breakage, with a large-scale structural transformation, in which a2 M uniquely entraps the proteinase in a cage-like structure and exposes receptor-binding domains for rapid endocytosis. Transformed a2 M ( a2 M-TR) contains up to two proteinases, which remain active to small substrates. 3-D electron microscopy is optimally suited to study this unusual structural change at resolutions near (1/30) Å−1. ^ The structural importance of the thiol esters was demonstrated by a genetically-engineered a2 M, with the cysteines involved in thiol ester formation mutated to serines, which appeared structurally homologous to a2 M-TR. This demonstrates that the four highly labile thiol esters alone maintain the a2 M-N structure, while the ‘closed trap’ formed by a2 M-TR is a more stable structural form. ^ Half-transformed a2 M ( a2 M-HT), with cleaved bait regions and thiol esters in only two of its four subunits, provides an important structural link between a2 M-N and a2 M-TR. A comparison with a2 M-N showed the two proteinase-entrapping domains were above and below the plane bisecting the long axis. Both a2 M-N and a2 M-TR consist of two dense, oppositely twisted strands with significant interconnections, indicating that the structural change involves a rotation of these strands. In a2 M-HT these strands were partially untwisted with large central openings, revealing the manner in which the proteinase enters the internal cavity of a2 M. ^ In reconstructions of a2 M-N, a2 M-HT and a2 M-TR labeled with a monoclonal Fab, the Fabs were located on distal ends of each constitutive strand, demonstrating an anti-parallel arrangement of the subunits. Separation between the top and bottom pairs of Fabs was nearly the same on all structures, but the pairs were rotated about the long axis. Taken together, these results indicate that upon proteinase cleavage the two strands in a2 M-N separate. The proteinase enters the structure, while the strands re-twist to encage it. In a2 M-TR, which displays receptor-binding arms, more than two subunits are transformed as strands in the transformed half of a2 M-HT were not separated. ^

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Grapholita molesta (Busck) es considerada plaga principal del duraznero en Mendoza. Sus larvas han sido criadas, en condiciones de laboratorio, con dietas naturales por diversos autores. Los objetivos fueron: 1. poner a punto la cría artificial de la especie; 2. diseñar una jaula para el apareamiento y la oviposición de los adultos; 3. evaluar la eficiencia de la jaula y la dieta larvaria mediante grados día, ciclo biológico, peso de pupas, recuperación de huevoadulto, fecundidad, viabilidad y longevidad. La cría artificial de una especie constituye una herramienta para profundizar sus conocimientos bioetoecológicos y, en consecuencia, aplicarlos en su control. En 1996 se fundó una cría con larvas salvajes alimentadas con manzanas verdes pequeñas del cv. Granny Smith. Los adultos se desarrollaron en una jaula especialmente diseñada. En el ciclo biológico, la recuperación huevo-adulto y la fecundidad se obtuvieron valores superiores a los citados por otros autores pero no ocurrió lo mismo con las otras variables. La cría artificial de Grapholita molesta (Busck) se logró desarrollar por 37 generaciones y la alta fecundidad obtenida es una clave fundamental en el éxito de su mantenimiento.

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Through a field experiment, we show that a predator has negative nonconsumptive effects (NCEs) on different life-history stages of the same prey species. Shortly before the recruitment season of the barnacle Semibalanus balanoides (May-June), we established experimental cages in rocky intertidal habitats in Nova Scotia, Canada. The cages were used to manipulate the presence and absence of dogwhelks, Nucella lapillus, the main predators of barnacles. At the centre of each cage, we installed a tile where barnacle pelagic larvae could settle and the resulting recruits grow. Mesh prevented caged dogwhelks from accessing the tiles, but allowed waterborne dogwhelk cues to reach the tiles. Results in May indicated that barnacle larvae settled preferentially on tiles from cages without dogwhelks. In November, at the end of the dogwhelk activity period and once the barnacle recruits had grown to adult size, barnacle body mass was lower in the presence of dogwhelks. This limitation may have resulted from a lower barnacle feeding activity with nearby dogwhelks, as found by a previous study. The observed larval and adult responses in barnacles are consistent with attempts to decrease predation risk. November data also indicated that dogwhelk cues limited barnacle reproductive output, a possible consequence of the limited growth of barnacles. Overall, this study suggests that a predator species might influence trait evolution in a prey species through NCEs on different life-history stages.

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Boron contents and boron, carbon and oxygen stable isotopes were determined for authigenic carbonates recovered from Ocean Drilling Program Leg 146, Oregon margin. Carbonate precipitates are the most widespread authigenic phase in the shallow accretionary wedge and carry chemical information about long-term variations in pore fluid origin and flow paths in the Cascadia subduction zone. Drilling the first ridge (toe area including the frontal thrust) and the second ridge (or Hydrate Ridge) of the prism demonstrated different fluid regimes, with higher B contents in the authigenic precipitates at the toe. The delta11B of 18 authigenic precipitates analysed ranges from 13.9 per mil to as high as 39.8 per mil, extending the upper range of previously reported carbonate delta11B values considerably. When related to the delta11B ratio of their parent solutions, these data are characteristic of fluid-related processes in accretionary prisms. Together with delta13C and delta18O, delta11B ratios of the carbonate concretions, nodules and crusts allow one to distinguish between precipitation influenced by (i) seawater, (ii) fluid reservoirs at different depth levels within the accretionary prism and (iii) cage water from dissociated gas hydrates, the latter possibly indicating a fluctuation of the bottom simulating reflector during most recent Earth's history. From this first systematic boron study on authigenic precipitates from an accretionary prism it is suggested that B contents of such carbonate crusts and concretions exceed those reported for other marine carbonates. Given the abundance of such precipitates at convergent margins, they represent a significant B sink in geochemical cycling. Isotopic compositions of the parent fluids to the carbonates mirror B chemistry of modern pore waters from convergent margins. The precipitates carry information of different subduction-related fluid processes over a certain period of time, and hence are a crucial tracer in the investigation of palaeo-fluid flow.

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Keel bone fractures and deviations are one of the major welfare and health issues in commercial laying hens. In non-cage housing systems like aviaries, falls and collisions with perches and other parts of the housing system are assumed to be one of the main causes for the high incidence of keel bone damage. The objectives of this study were to investigate the effectiveness of a soft perch material to reduce keel bone fractures and deviations in white (Dekalb White) and brown laying hens (ISA Brown) kept in an aviary system under commercial conditions. In half of 20 pens, all hard, metal perches were covered with a soft polyurethane material. Palpation of 20 hens per pen was conducted at 18, 21, 23, 30, 38, 44 and 64 weeks of age. Production data including egg laying rate, floor eggs, mortality and feed consumption were collected over the whole laying period. Feather condition and body mass was assessed twice per laying period. The results revealed that pens with soft perches had a reduced number of keel bone fractures and deviations. Also, an interaction between hybrid and age indicated that the ISA hybrid had more fractured keel bones and fewer non-damaged keel bones compared with the DW hybrid at 18 weeks of age, a response that was reversed at the end of the experiment. This is the first study providing evidence for the effectiveness of a soft perch material within a commercial setting. Due to its compressible material soft perches are likely to absorb kinetic energy occurring during collisions and increase the spread of pressure on the keel bone during perching, providing a mechanism to reduce keel bone fractures and deviations, respectively. In combination with genetic selection for more resilient bones and new housing design, perch material is a promising tool to reduce keel bone damage in commercial systems.

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On Sunday, Switzerland votes on its federal parliament. The members of both chambers - 200 National Councillors and 46 Councillors of States - are directly elected, with the 26 Swiss cantons (or provinces) forming the districts. Seats in the National Council are distributed to the cantons based on their population size, while in the Council of States each full canton gets two seats and the six half-cantons get one each. Here are six things to look out for.

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The effects of inclusion of pea hulls (PH) in the diet on growth performance, development of the gastrointestinal tract and nutrient retention were studied in broilers from 1 to 18d of age. There were a control diet based on low fibre ingredients (69.3 total dietary fibre (16.1g crude fibre/kg)) and three additional diets that resulted from the dilution of the basal diet with 25, 50 and 75g PH/kg (81.2, 93.2, and 105.1g total dietary fibre/kg diet, respectively). Each treatment was replicated six times and the experimental unit was a cage with 12 chicks. Growth performance, development of the gastrointestinal tract and the coefficients of total tract apparent retention (CTTAR) of nutrients were recorded at 6, 12 and 18d of age. In addition, jejunal morphology was measured at 12 and 18d and the coefficients of apparent ileal digestibility (CAID) of nutrients at 18d of age. Pea hulls inclusion affected all the parameters studied. The inclusion of 25 and 50g PH/kg diet improved growth performance as compared to the control diet. The relative weight (g/kg body weight) of proventriculus (P≤0.01), gizzard (P≤0.001) and ceca (P≤0.05) increased linearly as the level of PH in the diet increased. The inclusion of PH affected quadratically (P≤0.01) villus height:crypt depth ratio with the highest value shown at 25g PH/kg. In general, the CTTAR and CAID of nutrients increased linearly and quadratically (P≤0.05) with increasing levels of PH, showing maximum values with PH level between 25 and 50g/kg diet. We conclude that the size of the digestive organs increases with increasing levels of PH in the diet. In general, the best performance and nutrient digestibility values were observed with levels of PH within the range of 25 and 50g/kg. Therefore, young broilers have a requirement for a minimum amount of dietary fibre. When pea hulls are used as a source of fibre, the level of total dietary fibre required for optimal performance is within the range of 81.2–93.2g/kg diet (25.6–35.0g crude fibre/kg diet). An excess of total dietary fibre (above 93.2g/kg diet) might reduce nutrient digestibility and growth performance to values similar to those observed with the control diet.

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En la presente Tesis Doctoral se ha estudiado el efecto de una metodología para inducir la muda en dos estirpes de gallinas ponedoras comerciales, una ligera y otra semipesada, mediante el suministro de tres alimentos: salvado de trigo, cebada en grano y un pienso comercial de ponedoras aportado en cantidad restringida. Se realizaron dos pruebas en dos lotes diferentes de gallinas. En la primera de ellas se utilizaron 472 animales (236 de cada estirpe) alojados en jaulas con cuatro o seis gallinas por jaula, con una estructura factorial 2 x 3 x 2 (2 estirpes, 3 alimentos, 2 densidades) y una duración total de 32 semanas (4 de muda y 28 de producción posmuda). En la segunda prueba fueron 432 animales los utilizados (216 de cada estirpe), alojados en grupos de cuatro aves por jaula, con una estructura factorial 2 x 3 (2 estirpes, 3 alimentos) y una duración de 27 semanas (4 de muda y 23 de producción postmuda). En los dos experimentos realizados se estudió el efecto del uso de los alimentos citados para inducir de la muda sobre los resultados cuantitativos: pérdida de peso vivo durante la muda e intensidad de puesta, peso medio del huevo y masa de huevo diaria, durante y después de la muda, así como la distribución de la puesta en clases comerciales durante el segundo ciclo de puesta. Así como sobre los resultados cualitativos después de la muda: color de la cáscara de los huevos morenos, espesor de la cáscara, peso específico del huevo, altura del albumen, unidades Haugh y color de la yema. En la primera prueba se estudió, además, el efecto que la inducción de la muda mediante los tres alimentos considerados tuvo sobre la regresión del aparato reproductor de las gallinas durante este período de muda. En el primer experimento se observaron diferencias entre estirpes. Las gallinas ligeras tuvieron una más rápida regresión del aparato reproductor (ovario+oviducto) (P=0,003) que las semipesadas, aunque la regresión total no presentó diferencias significativas. Las gallinas semipesadas tuvieron mejores resultados después de la muda en intensidad de puesta (P<0,0001), en peso medio del huevo (P<0,0001) y en masa de huevo diaria (P=0,0002). También hubo diferencias significativas para las variables cualitativas espesor de la cáscara (mayor en huevos de gallinas semipesadas) mientras que los huevos procedentes de gallinas ligeras presentaron mejores valores de altura del albumen, de unidades Haugh y de color de yema; todas estas variables tuvieron un nivel de significación P<0,0001. El suministro restringido de pienso dio lugar a un mayor porcentaje de pérdida de peso vivo (P<0,0001) aunque la regresión de los órganos del aparato reproductor fue la más baja (P<0,003), no habiéndose encontrado diferencias entre los otros dos alimentos utilizados. Con este alimento también fue más lenta (P<0,0001) la disminución de la puesta durante la muda, aunque fue mayor la producción durante el segundo ciclo (P<0,0001). La única variable cualitativa afectada fue el espesor de cáscara (P<0,0001), con valores más altos en los huevos producidos por las gallinas mudadas con cebada. Los grupos de seis gallinas por jaula produjeron más huevos durante la muda (P<0,0001) aunque después de ésta la densidad de animales no tuvo efecto significativo, como tampoco lo hubo sobre los parámetros de calidad del huevo. En la segunda prueba las gallinas semipesadas experimentaron un menor porcentaje de pérdida de peso corporal (P<0,01) pero tuvieron mayores índices de puesta (P<0,001) y de huevos clasificables (P<0,001) durante la muda. En cambio, durante el segundo ciclo de producción las gallinas ligeras produjeron más huevos (P=0,0041), de menor peso (P<0,02) y con menor consumo de pienso (P<0,001). Los huevos puestos por las gallinas semipesadas tuvieron mayor espesor de cáscara y mayor color de yema, pero peor calidad de albumen (P<0,0001). La mayor pérdida de peso la experimentaron las gallinas mudadas con salvado (P<0,02). La producción durante la muda fue mayor (P<0,001) en las gallinas que consumieron pienso en cantidad restringida y también fueron las que tuvieron menor intensidad de puesta (P<0,006) y masa de huevo diaria (P<0,042) durante el segundo ciclo. El tratamiento de muda no tuvo efecto significativo sobre la calidad del huevo en esta segunda prueba. La principal conclusión que merece destacarse es que es posible inducir la muda a las gallinas ponedoras utilizando alimentos bajos en energía o en proteína, o altos en fibra, con un porcentaje de pérdida de peso vivo no tan alta como las recomendaciones tradicionales, y alcanzar buenos resultados productivos, tanto cuantitativos como cualitativos, durante el segundo ciclo de puesta. ABSTRACT Present Doctoral Thesis has studied the effect of a methodology to induce molting in two strains of commercial laying hens, one light and another semi-heavy one, through the provision of three feed: wheat bran, barley grain and a commercial laying hens feed provided in limited quantity. Two tests were performed in two different lots of layers. In the first 472 animals were used (236 of each strain) housed in cages with four or six hens per cage, with a structure 2 x 3 x 2 factorial (2 strains, 3 meals, 2 densities) and a total duration of 32 weeks (4 of molt and 28 of postmolting). In the second test 432 animals were used (216 each strain), housed in groups of four birds per cage, with a structure factorial 2 x 3 (2 strain, 3 meals) along 27 weeks (4 of molt and 23 of postmolting). In both experiments, we studied the effect of the use of above mentioned foods to induce molting on the quantitative results: body weight lost during the molt and laying index, average egg weight and egg mass daily during and after the molt, as well as on grading in commercial classes during the second laying cycle. As well as on qualitative outcomes after the molt: colour of Brown eggsshell, shell thickness, specific density, albumen height, Haugh units and yolk colour. In the first test was studied, in addition, the effect of induction of molting through the three feed considered on the regression of the reproductive tract of hens during molting period. In the first experiment, differences between strains were observed. Light hens had a faster regression of the reproductive tract (ovary+oviduct) (P=0,003) than semi-heavy hens, although the total regression did not present significant differences. Semi-heavy hens had better outcomes after the molt in laying index (P<0.0001), in average egg weight (P<0.0001) and daily egg mass (P=0, 0002). There were also significant differences for the qualitative variables (higher in semi-heavy hen eggs) as shell thickness while light chicken eggs showed better values of albumen height, Haugh units and yolk color; all these variables had the same level of significance (P<0.0001). Restricted supply of layer feed resulted in a greater percentage of live weight loss (P<0.0001) although the regression of the reproductive organs was the lowest (P<0.003), having not found differences between the other two feed used. With this food decreasing of laying during molting period was also slower (P<0.0001), although production was higher during the second cycle (P < 0.0001). The only qualitative variable affected was shell thickness (P<0.0001), with higher values in the eggs produced by hens molted with barley. Groups of six hens per cage produced more eggs during the moult (P<0.0001) but after this animal density had no significant effect, as neither had it on egg quality parameters. In the second trial hens semi-heavy experienced a lower percentage of body weight loss (P<0.01) but had higher rates of egg production (P<0.001) and of grading eggs (P<0.001) during the moult. On the other hand, during the second production cycle light hens produced more eggs (P=0,0041), of lower weight (P < 0.02) and with less feed intake (P<0.001). Eggs from semi-heavy hens had thicker shell and greater color yolk, but poorer quality of albumen (P<0.0001). The greater weight loss was experimented by the hens molted with wheat bran (P=0.02). Production during molting period was greater (P<0.001) in hens which consumed feed in restricted quantities and were also less the laying index (P=0.006) and daily egg mass (P=0.042) during the second cycle. The molting treatment had no significative effect on the quality of the egg in this second test. The main conclusion that deserves to stand out is that it is possible to induce molting hens using foods low in energy or in protein or high in fiber, with a percentage of live weight loss not as high as the traditional recommendations, and achieve good productive, both quantitative and qualitative results during the second implementation cycle.