Frequency of human leukocyte antigen (hla) in patients with malaria and in the general population of Humaitá county, Amazonas state, Brazil

Em agosto de 1983 foram observados 85 habitantes do Município de Humaitá, Estado do Amazonas, Brasil, com a finalidade de estudar a prevalência dos antígenos de HLA -A, -B, -C e DR, dentre os quais 38 eram doentes com malária causada pelo Plasmodium falciparum Todos eles foram examinados para avaliação de esplenomegalia, exame parasitológico de sangue e pesquisa de anticorpos de malária. Foram constituídos três grupos: (I) 25 indivíduos nascidos na região Amazônica que nunca tiveram malária; (II) 38 indivíduos naturais da Amazônia que tinham sido tratados de malária no passado, ou que estavam tendo malária atual, e (III) 22 doentes com malária que contraíram na Amazônia e eram procedentes de outras regiões do Brasil. Foram colhidas amostras de sangue de cada um deles, separados os linfôcitos e os antígenos de HLA foram tipados pelo teste de microlinfocitotoxidade. Houve elevada freqüência de antígenos não identificados, nos grupos estudados, o que sugere ou a existência de homozigoze, oufenôtipo não identificado nessa população. Houve alta freqüência fenotípica de antígeno deAg(W24) (44,7%) no Grupo II, quando comparado ao Grupo 1(32%) ou Grupo III (9%). Os indivíduos do Grupo II mostraram também elevada freqüência do antígeno DR4 (80%) quando comparado ao Grupo 1(36,3%) ou Grupo III(16,6%). Essas observações sugerem a possibilidade de suscetibilidadegenética ã malária entre os nativos da Amazônia e indicam a necessidade da realização de inquéritos mais extensos sobre a freqüência de antígenos de HLA em habitantes de zona endêmica de malária.

Evaluation of ten tropical grasses in the northwest region of the state of Sao-Paulo Brazil

The experiment was carried out in the northwest region of the State of São Paulo-Brazil to evaluate ten grasses recently introduced in the region. The grasses studied were: Cynodon nlemfuensis cv. Tifton 68, C dacrylon cv. Tifton 78, Cynodon spp. cv. Tifton 85, C. dactylon cv. Florakirk, C. nlemfuensis cv. Florico, C nlemfuensis cv. Florona, C dactylon cv. Coastcross, Paspalum notation cv. Tifton and NDF (717 to 749 g.kg(-1)) were observed in the Cynodon cultivars while lower values of CP (102 and 107 g.kg(-1)) and NDF (697 and 705 g.kg(-1)) were registered in the cvs. Marandu and Tanzania 1. The contents of ADF were higher in the the cvs. Tanzania 1 and Tifton 9. The average values of IVDMD differed (Ppound0.05) among grasses and were all above 564 g.kg(-1), except for cv. Tifton-9 (499 g.kg(-1)). The results obtained in this work allowed to conclude that the cvs. Tifton 78, Tifton 85, Coastcross, Florona, and Tanzania 1 are interesting options for pastures establishment in the northwest region of the State of São Paulo-Brazil.

Cryptosporidium spp. infection in mares and foals of the northwest region of São Paulo State, Brazil

O presente estudo teve como objetivo analisar a ocorrência da infecção por Cryptosporidium spp. em éguas e seus respectivos potros. Este estudo foi realizado em 11 fazendas localizadas nos municípios de Araçatuba, Birigui, Guararapes e Santo Antônio do Aracangua, na região Noroeste do Estado de São Paulo, de novembro de 2010 a março de 2011. Um total de 98 éguas e 98 potros de diversas raças foram analisados, sendo que, entre os filhotes, 59 eram machos e 39 fêmeas, cujas idades variavam de três até 330 dias. Fezes foram colhidas diretamente da ampola retal, purificadas e processadas pela técnica de Kinyoun modificada. A ocorrência de Cryptosporidium spp. observada foi de 21,4% (21/98) para potros e 18,4% (18/98) para éguas. A ocorrência de Cryptosporidium spp. teve uma associação significativa com a raça e a idade dos animais. A partir dos resultados obtidos, conclui-se neste estudo que potros com idade superior a dois meses e animais da raça Mangalarga foram menos susceptíveis à ocorrência de Cryptosporidium spp.

Insights into the role of oligomeric state on the biological activities of crotoxin: crystal structure of a tetrameric phospholipase A(2) formed by two isoforms of crotoxin B from Cratalus durissus terrificus venom

Crotoxin B (CB or Cdt PLA(2)) is a basic Asp49-PLA(2) found in the venom of Crotalus durissus terrificus and it is one of the subunits that constitute the crotoxin (Cro). This heterodimeric toxin, main component of the C. d. terrificus venom, is completed by an acidic, nontoxic, and nonenzymatic component (crotoxin A, CA or crotapotin), and it is related to important envenomation effects such as neurological disorders, myotoxicity, and renal failure. Although Cro has been crystallized since 1938, no crystal structure of this toxin or its subunits is currently available. In this work, the authors present the crystal structure of novel tetrameric complex formed by two dimers of crotoxin B isoforms (CB1 and CB2). The results suggest that these assemblies are stable in solution and show that Ser1 and Glu92 of CB1 and CB2, respectively, play an important role in the oligomerization. The tetrameric and dimeric conformations resulting from the association of the isoforms may increase the neurotoxicity of the toxin CB by the creation of new binding sites, which could improve the affinity of the molecular complexes to the presynaptic membrane.

Persistent organic pollutants in juvenile Magellan penguins (Spheniscus magellanicus) found on the northern shore of the state of São Paulo and southern shore of the state of Rio de Janeiro, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ecological aspects of hermit crabs (Crustacea, Anomura, Paguroidea) off the northern coast of São Paulo State, Brazil

The occurrence of species of hermit crabs and their ecological distribution in soft bottoms off Ubatuba, São Paulo, Brazil were analyzed. To better understand the distribution of the species in relation to environmental factors, the similarity and species-diversity indexes were calculated. Paguroideans were sampled monthly from January through December 2000. The trawls were made with two otter-trawl nets at 13 different sites, at depths of 2-40 m. Water temperature, salinity, sediment texture, and organic matter content were measured. Gastropod shells occupied by hermit crabs were also assessed. A total of 1,238 specimens was collected, belonging to the families Diogenidae and Paguridae, comprising seven genera and thirteen species. The most abundant hermit crab species were Dardanus insignis (761 specimens) and Loxopagurus loxochelis (351 specimens). Phimochirus holthuisi is newly reported from the São Paulo coast. The highest diversity index was found for the shallower sites near rocky shores. The results of the grouping analysis for sites and species indicated three distinct groups for sites, and four groups for species. This suggests that the occurrence of these anomurans is associated with the environmental and biotic factors analyzed.

Biodiversity of brachyuran crabs (Crustacea : Decapoda) from non-consolidated sublittoral bottom on the northern coast of São Paulo State, Brazil

The brachyuran community of the coast of São Paulo State is represented by about 188 species of crabs inhabiting different kinds of coastal marine environments. The biodiversity of brachyurans found on non-consolidated sublittoral bottom was investigated. The Ubatuba region (Ubatumirim, Ubatuba and Mar Virado bays, Couves and Mar Virado Islands, offshore region) was sampled for 3 years (1998-2000), at depths of 2-40 m. All sampling was performed using a fishing boat equipped with two double-rig nets. We collected 79 brachyuran species representing 9 superfamilies (4 Dromioidea, 1 Homoloidea, 2 Calappoidea, 5 Leucosioidea, 20 Majoidea, 7 Parthenopoidea, 17 Portunoidea, 18 Xanthoidea, and 5 Pinnotheroidea) and 41 genera. Ubatuba bay showed the greatest species richness with 50 species, followed by Ubatumirim with 45 and Mar Virado with 29. The number of species collected represents about 57% of the known species of crabs already reported for the shore of São Paulo State. It is worth noticing that this percentage is restricted only to non-consolidated sublittoral bottom. This fact indicates a great biodiversity of the habitat in this studied region, probably one to the diversity of habitat types present in the bays.

Ecological distribution and reproductive period of the hermit crab Loxopagurus loxochelis (Anomura, Diogenidae) on the northern coast of São Paulo State, Brazil

The present study aimed to determine the ecological distribution of Loxopagurus loxochelis as a function of selected environmental factors, as well as its reproductive period based on the combined analysis of the presence of ovigerous females and the development of the gonad. The collections were carried out monthly from January to December 2000 in the region of Ubatuba (SP), using a fishing boat equipped with two double rig nets, in isobaths of 5, 10, 15, 20, 25, 30, 35 and 40 m, at which depths samples of sediment and water were collected. The cephalothoracic shield length and sex of the animals were determined; the abdomen was dissected to verify gonad development stages. A total of 334 individuals was obtained ( 196 males, 48 ovigerous females and 90 non-ovigerous females) with sizes varying from 3.0 to 8.6 mm (5.7 +/- 1.0 mm). A greater abundance of L. loxochelis (95.2%) was observed at depths of 20 and 25 m. These sites mainly revealed a substratum stable with a very fine sand fraction, which facilitates the habit of embedding shown by this hermit. With regard to gonadal analysis, it was possible to classify four gonad development stages for each sex: immature, rudimentary, developing and developed. It was observed that in the winter months about 71% of the females had gonads either developing or developed and 67% were ovigerous. Therefore, the winter period can be considered the peak of reproduction for this species, characterizing a typical seasonal-continuous reproduction.

Abundance and ecologic distribution of the shrimp Rimapenaeus constrictus (Crustacea : Penaeidae) on the northern coast of São Paulo State, Brazil

The abundance and ecologic distribution of the shrimp R. constrictus were compared among three different embayments within the northern coast of São Paulo State (23degreesS, 45degreesW); i.e. Mar Virado (MV), Ubatuba (UBA) and Ubatumirim (UBM). Key environmental factors were monitored for a better understanding of the distributional patterns of this species, namely temperature, salinity, depth, organic matter content and texture of sediments. In each bay, six transects were delimited, four parallel to the coastline and the other two in areas adjacent to rocky shores. Monthly samples were taken over a 2-year period (1998 and 1999), using a shrimp fishing boat equipped with double-rig nets. Abundance data were treated separately according to environmental characteristics. A total of 4978 specimens was obtained; 921 at MV, 1948 at UBA and 2109 at UBM. The spatial distribution of R. constrictus differed among bays. Higher abundance values were recorded at areas where silt and clay comprised less than 70% of the sediment. A more diversified sediment at UBM and UBA apparently favours the establishment of this species in the region. Its abundance also followed a seasonal trend, higher during spring and summer when intrusions of cold South Atlantic Coastal Waters (SACW) are frequent, promoting the migration of this shrimp to more sheltered areas. These results suggest that sediment type and temperature are the most important variables affecting the spatial and seasonal distribution of this species.

Morphometry of the crab Hexapanopeus schmitti (Decapoda : Xanthoidea) on the northern coast of the state of São Paulo, Brazil

A morphometric study of the xanthoid crab Hexapanopeus schmitti was carried out, using the allometric method. Samples were taken monthly for two years (1998-1999) in the Ubatuba region, northern coast of São Paulo, Brazil. Sex and size were assessed for each specimen, and all crabs were measured to obtain their carapace width (CW) and length (CL), abdomen width (AW) of females, major cheliped propodus length and height (PL and PH), and gonopod length (GL) of males. A total of 301 crabs were analyzed, 209 males and 92 females. The CWs of the crabs ranged from 2.5 to 9.8 mm for males and from 2.8 to 9.4 mm for females. The relative growth equation (y=ax(b)) based on the relationship between GL and CW suggested that males reach their morphological sexual maturity near 6.1 mm CW. In females, the estimated size at 50 % maturity was 4.8 mm CW, based on the relationship of AW vs. CW. Males reach larger sizes than females, which probably favors their ability to guard the females during courtship. In approximately 83 % of the crabs (n= 371), disregarding sex, the right cheliped was larger.

Population structure and breeding period of Pachycheles monilifer (Dana) (Anomura, Porcellanidae) inhabiting sabellariid sand reefs from the littoral coast of São Paulo State, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Abundance and distribution of juvenile pink shrimps Farfantepenaeus spp. in a mangrove estuary and adjacent bay on the northern shore of São Paulo State, southeastern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Low-Resolution Molecular Models Reveal the Oligomeric State of the PPAR and the Conformational Organization of Its Domains in Solution

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Primary production of Utricularia foliosa L., Egeria densa Planchon and Cabomba furcata Schult & Schult.f from rivers of the coastal plain of the State of São Paulo, Brazil

Seasonal variation in gross primary production (GPP) of Utricularia foliosa Linnaeus, Egeria densa Planchon and Cabomba furcata Schult & Schult.f. in rivers of the coastal plain of the state of São Paulo, Brazil was examined in relation to water physico-chemistry. These three species do not affect the multiple uses of the streams and are present throughout the year. The most productive was U. foliosa (maximum production 24.7 mgO(2) g(-1) DW h(-1)), while C. furcata had an intermediate GPP (maximum production 17.5 mgO(2) g(-1) DW h(-1)) and E. densa was lowest at 5.6 mgO(2) g(-1) DW h(-1). Despite the low amplitude of seasonal variation in this south tropical area, the three species showed seasonal variation in the primary production: GPP was positively correlated with photosynthetic active radiation for U. foliosa and E. densa, and there was a negative correlation for C. furcata. For U. foliosa, GPP was positively correlated with temperature and dissolved inorganic carbon and the GPP of C. jurcata was positively correlated with dissolved inorganic carbon.

Reproduction of the fish community of Passa Cinco Stream, Corumbataí River sub-basin, São Paulo State, Southeastern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)