Copper-nickel alloys for marine aquacultural engineering

Engineering developments, problems of marine corrosion, fouling and practical innovative applications of copper-nickel alloys in comparison with other materials for marine aquacultural engineering are presented. Coefficient of drag and hydrodynamic force acting on nylon and polyethylene net enclosures have shown a four-fold increase of force in four months at the Cochin harbour due to fouling. Corrosion behaviour of copper-nickel and other copper base alloys are also presented. Effects of copper additions to culture water are reviewed.

Fish farm technology. 1 - Brackishwater fish culture based on "aquafeed"

A method has been evolved to enhance the production of natural feed in brackish water fish farms by providing substrates for bio-growth ('aquafeed' production) which is a biomass complex consisting of sedentary and associated organisms of plant and animal species. The seasonal fluctuations of the aquafeed production over different substrates ranged as: 787-1830g/coconut leaf (6m²)/45 days, 16.0-072.9g/glass panel (2x10x10cm²)/30 days, 52-230g/nylon mat (2x25x25cm²)/30 days and 18.6-123.1g/wooden block (6x10x10cm²)/30 days. The average dry weight composition of the major components of aquafeed obtained in the present study was sand-silt-clay 40%, protein 22%, carbohydrate (water soluble) 1.8% and fat 3.35% (water content 85%). Mugil cephalus of 1.85 cm reared in a 0.01ha pond and fed on aquafeed attained a size of 23 cm length and 146.73g weight during one year. Survival rate was 54% at a density of 1000/ha. Salinity and temperature of the pond during the culture period ranged between 1.4 and 32.8‰ and 28.1 and 36.5°C respectively.

Survival rates of different postlarval stages of Penaeus monodon Fabricius

The objective of this study is to determine survival rates of different postlarval stages upon stocking in the Leganes ponds. Twelve 3m x 2m x 2m suspension nets made of nylon cloth (mesh size = 0 . 1 mm) were set up in a Leganes Station pond (ave. water depth = 1 m) by means of 3-m long poles stacked at distances approximating the area of each net. The net bottom was filled with topsoil at least 15 cm thick to stimulate the pond bottom. At least 60 cm of the upper edge of each net was above the water level to prevent mixing of water inside and outside the net. P.monodon of stages P SUB-11 , P SUB-15 , P SUB-21 (from the hatchery) and P SUB-25 (from the wet lab) were stocked in the nets at 200/sq m or 1,200 fry/net. Due to lack of fry, only one P SUB-25 net was stocked. Each net had two large dried miapi branches as shelter from predation and cannibalism for the young sugpo fry. Fresh lablab was fed at the rate of one pail (approximately 5 kg) every four days per net. Harvest data show relatively higher survival rates for P SUB-15 and P SUB-18 compared to P SUB-11 and P SUB-25 with no significant difference between these two stages. The results for P SUB-25 may not be valid because the stock came from the wet lab in comparison to the other postlarval stages which were reared in the hatchery. Moreover, the P SUB-25 stock had no replicates and the net itself (no. 10) was discovered to have many holes. These preliminary results point to P SUB-15 as the best stage for harvest from the hatchery in terms of high pond recovery and lesser expense in rearing compared to older postlarvae.

Studies on the suitability of HDPE material for gill nets

The suitability of HDPE yarn and HDPE twine in place of nylon for gill nets has been studied. As regards total catch nylon gill net is found to be better than HDPE nets. However, statistical analysis of the catch in respect of quality fishes shows that HDPE yarn nets are equally efficient as nylon nets.

Species diversification in coastal aquaculture: production potentials of shrimp (Penaeus monodon) with mono and mixed sex tilapia

An experiment of 120 days of culture was conducted in brackishwater earthen ponds having an area of 0.2ha each. The hatchery produced shrimp (Penaeus monodon) post larvae were stocked in the 40m² fine meshed nylon net nursery enclosures were fed with commercial pellet feed. After two weeks of nursing, juveniles were allowed to spread in cultural pond by opening the fence. Fingerlings of three different strain of tilapia were stocked as shrimp and Strain-1 all male (monosex) (T1), shrimp and Strain-2 all male (T2), shrimp and Strain-3 mixed sex population (T3) @ 20.000/ha and 10.000/ha, respectively and shrimp only (monoculture) (T4) @ 20.000/ha. The shrimp and fish were fed with farm made feed consisting of a mixture of fishmeal 29%, MOC 15%, rice bran 30%, soybean meal 16%, wheat flour 9% and vitamin premix 0.1%. The average final weight of shrimp was 24.9±1.13g, 23.41±3.26g and 26.67±1.89g that stocked with tilapia in treatments T1, T2, and T3 respectively. The final average weight of shrimp in monoculture (T4) was 27.41±0.76g, apparently higher but insignificant in treatments. The survival of shrimp was 42.17%, 32.38%, 39.45% and 61.98% in treatments T1 T2, T3 and T4 respectively. The production of shrimp in concurrent culture was 193.67, 154.26 and 210.41kg/ha in T1, T2 and T3, respectively, while in monoculture (T4) was 339.77 kg/ha. The growth and survival of tilapia among the treatments was insignificant. The growth of monosex tilapia ranged 225.29 and 291.31g and survival 62.77 and 72.20% in T1 and T2, respectively, in mixed sex was 193.0g and 83.20% (T3). The production of tilapia monosex strains was 1676.69kg/ha (Strain-2 all male) and 1668.98 kg/ha (Strain-1 all male) while that of Strain-3 mixed sex population was 1622.92 kg/ha.

Effects of supplemental feed and fertilizer on growth and survival of Macrobrachium rosenbergii (de Man) post larvae in pond nursery system

The study was conducted to compare the performance of different nursing practices of giant freshwater prawn (Macrobrachium rosenbergii) post-larvae (PL). Three treatments such as only fertilizers (T1), fertilizers with 5% supplementary feed (local feed) (T2), and 10% commercial feed (T3) were applied in the nursing system of prawn PLs in earthen pond. An earthen pond (315m²) was divided into nine equal small ponds by fine meshed nylon nets. Feeds were used once daily on a tray placed near the pond bottom. There was a significant difference (p<0.05) in some water quality parameters like pH and total alkalinity, but all measured water quality parameters viz. water temperature, transparency, dissolved oxygen and ammonia-nitrogen were within the acceptable range for nursing of prawn PL. The results showed that the mean final lengths of prawn post-larvae were 6.3±0.07 cm, 7.12±0.22 cm and 8.17±0.16 cm in T1, T2 and T3, respectively. There were significant difference (p<0.05) in mean final length of prawn PL among the treatments. Significantly higher (p<0.05) average daily weight gain was observed in T3 (0.071 ±0.007 g) than in T2 (0.052±0.006 g) and T1 (0.031 ±0.002 g). The specific growth rate (SGR) of T3 (8.81±0.26) was found significantly higher (p<0.05) than T2 (8.35±0.22) and T1 (7.42±0.11). Survival rate (%) was also significantly higher (p<0.05) in T3 (66.24±1.58) than in T2 (60.52±1.64) and T1 (53.86±2.71). Therefore, it may be concluded that the growth and survival in prawn nursery was better in commercial feed than only fertilizers and fertilizers with local feeds.

Trolling for Nile perch (Lates niloticus) in Lake Kyoga

Nile perch were introduced into Lake Kyoga in the mid·1950s from Lake Albert. Murchison Falls on the River Nile, between the two lakes, prevented Nile Perch and other elements of the typical nilotic fish population from naturally reaching Lake Kyoga. The introduction has been successful and considerable stocks of Nile Perch now exist in Lake Kyoga. In 1967, 13,000 tons of Nile Perch were estimated to have been landed by the commercial fishermen, fish of 200 lb. being now caught and specimens of 100 lb. being fairly common. Large Nile perch are caught commercially on long lines baited with live Protopterus' spp. or Clarias spp. Large mesh gillnets uccasionally take Nile Perch of up to 30 lb., but the high cost of the nets does not, at the moment, appear to justify this method of fishing; a 10 in. net, stretched 100 yards long (unmounted). 15 meshes deep and 60-ply nylon. costs approximately U. Shs. 300. The long·lines used are extremely simple and cheap to make, but considerable labour is needed to catch bait. Small Protopterus are normally caught by turning over floating rafts of grasses and papyrus, and extracting the fish from the root mass; this is hard and dirty work. Other small fish, more readily available, do not, according to fishermen, work as well, possibly because they are not as durable as the Protopterus or Clarias. Dead bait is never used.

A discussion of possible management methods to revive the Labeo victorianus fishery of Lake Victoria, with special reference to the Nzoia river, Kenya

Labeo victorianus Boulenger, the "ningu", is commercially the most important migratory fish of Lake Victoria, as well as being one of the most abundant of all species landed. Annual catch records of the Fisheries Departments of Uganda, Kenya and Tanzania show that a high yielding seasonal, floating gill net fishery is based on the concentration of sexually mature fish at the river mouths at the time of migration during the bi-annual floods. Migrating fish used also to be caught in high numbers at "kek" barrier traps across the river, as at Hainga on the Nzoia river. Since the heavy exploitation at the river mouth which occurred with the introduction of nylon gill nets in 1956.

Fishery resource assessment and monitoring in the development and control of fisheries in the Lake Volta

Gill-netting and rotenoning have been used for assessing and monitoring fish stock abundance in Volta Lake. The lake and the main gear types used on it have been described. Before a gill-net sampling plan was set up, a preliminary survey was undertaken which largely determined the final form of the plan. An investigation as to whether or not the lake was being overfished concluded that it was being underfished. Commercial and experimental catch data analyses disclosed that the adults of the small species were being little utilized. Commercial sized species were also not being harvested according to their apparent proportion in the population. Production is presently fluctuating between approximately 37,000 and 40,000 tonnes. A high correlation between commercial and experimental catch was realized. Developments which have followed in the wake of stock assessment and monitoring studies include: introduction of monofilament nylon net, development of a special scoop net to permit mass harvest of clupeids after they have been attracted to light, and the design of a larger canoe which would help to extend the fishery into open water. New regulation and management policies will have to be formulated in the light of new findings before a rational exploitation of all the species can be achieved.

Lakes Wamala and Kijanebalola: evaluation of the fisheries resources during July 1975

This report presents the results of the second (in 1975) fisheries resource survey for Lake Wamala conducted from 10th to 16th July 1975. The first similar survey covered the period 14th to 20th May 1975. The areas sampled consisted of the river-month areas, the papyrus-fringed inshore waters and the open dee offshore waters. In an effort to find the possible major causes of the decline in catch and seasonal disappearance of fish-hence a solution to the problem(s)-a second fisheries resource survey using multifilament nylon gillnets was conducted on Lake Kijanebalola during the period 17th to 21st July 1975. The first survey was similar and covered the period 21st to 27th May 1975.

Deep mixing for excavation support: Design issues

Deep mixing is effectively used in excavations both in conjunction with and in substitution of traditional techniques, where it results in more economical and convenient solutions for the stability of the system and the prevention of seepage. Deep mixed walls constructed as part of a soldier pile and tie-backs system act also as temporary support, prevent seepage like a sheet pile wall, but require a lower amount of steel. The deep mixed treatment can also contribute to the stability of the wall system against deep-seated failures. Although deep mixing is currently used for excavation control in numerous projects, no standard procedure has been developed and the different applications have not been evaluated. As this technique emerges as a more economical and effective alternative to traditional excavation shoring, there is a need for guidelines describing proven procedures for evaluation of design, analysis and construction. This paper presents comparisons in the design of excavation support using deep mixing and other traditional techniques. Issues important for design, analysis, and construction of deep mixed excavation walls are also discussed.

Efficient statistical electromagnetic analysis in reverberant environments

This paper presents a statistical approach to the electromagnetic analysis of a system that lies within a reverberant cavity that has random or uncertain properties. The need to solve Maxwell's equations within the cavity is avoided by employing a relation known as the diffuse field reciprocity principle, which leads directly to the ensemble mean squared response of the system; all that is required is the impedance matrix of the system associated with radiation into infinite space. The general theoretical approach is presented, and the analysis is then applied to a five-cable bundle in a reverberation room © 2013 EMC Europe Foundation.

Localization and tracking of phonating finless porpoises using towed stereo acoustic data-loggers

Cetaceans produce sound signals frequently. Usually, acoustic localization of cetaceans was made by cable hydrophone arrays and multichannel recording systems. In this study, a simple and relatively inexpensive towed acoustic system consisting of two miniature stereo acoustic data-loggers is described for localization and tracking of finless porpoises in a mobile survey. Among 204 porpoises detected acoustically, 34 individuals (similar to 17%) were localized, and 4 of the 34 localized individuals were tracked. The accuracy of the localization is considered to be fairly high, as the upper bounds of relative distance errors were less than 41% within 173 m. With the location information, source levels of finless porpoise clicks were estimated to range from 180 to 209 dB re 1 mu Pa pp at 1 m with an average of 197 dB (N=34), which is over 20 dB higher than that estimated previously from animals in enclosed waters. For the four tracked porpoises, two-dimensional swimming trajectories relative to the moving survey boat, absolute swimming speed, and absolute heading direction are deduced by assuming the animal movements are straight and at constant speed in the segment between two consecutive locations.

Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide

Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subsequently identified as a vitally induced gene. The full length cDNA of PoCatB is 1801 bp encoding 330-amino acids. The deduced protein has high homology to all known cathepsin B proteins, containing an N-terminal signal peptide, cysteine protease active sites, the occluding loop segment and a glycosylation site, all of which are conserved in the cathepsin B family. PoCatB transcription of FEC cells could be induced by turbot (Scophthalmus maximus) rhabdovirus (SMRV), UV-inactivated SMRV, UV-inactivated GCHV, poly I:C or lipopolysaccharide (LPS), and SMRV or poly I:C was revealed to be most effective among the five inducers. In normal flounder, PoCatB mRNA was detectable in all examined tissues. Moreover, SMRV infection could result in significant upregulation of PoCatB mRNA, predominantly in spleen, head kidney, posterior kidney, intestine, gill and muscle with 18.2,10.9, 24.7,12, 31.5 and 18 fold increases at 72 h post-infection respectively. These results provided the first evidence for the transcriptional induction of cathepsin B in fish by virus and LPS, indicating existence of a novel function in viral defense. (C) 2008 Elsevier Ltd. All rights reserved.

Molecular characterisation and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection

Protein arginine methyltransferase 1 (PRMT1) is currently thought as an effector to regulate interferon (IFN) signalling. Here Paralichthys olivaceus PRMT1 (PoPRMT1) gene was identified as a vitally induced gene from UV-inactivated Scophthalmus maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). PoPMRT1 encodes a 341-amino-acid protein that shares the conserved domains including post-I, motif I, II and III. Homology comparisons show that the putative PoPMRT1 protein is the closest to zebrafish PMRT1 and belongs to type I PRMT family (including PRMT1, PRMT2, PRMT3, PRMT4, PRMT6, PRMT8). Expression analyses revealed an extensive distribution of PoPMRT1 in all tested tissues of flounder. In vitro induction of PoPRMT1 was determined in UV-inactivated SMRV-infected FEC cells, and under the same conditions, flounder Mx wash also transcriptionally up-regulated, indicating that an IFN response might be triggered. Additionally, live SMRV infection of flounders induced an increased expression of PoPRMT1 mRNA and protein significantly in spleen, and to a lesser extent in head kidney and intestine. Immunofluorescence analysis revealed a major cyptoplasmic distribution of PoPRMT1 in normal FEC but an obvious increase occurred in nucleus in response to UV-inactivated SMRV. This is the first report on in vitro and in vivo expression of fish PRMT1 by virus infection, suggesting that PoPRMT1 might be implicated in flounder antiviral immune response. (c) 2006 Elsevier Ltd. All rights reserved.