Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media

The purpose of this work was to compare and optimise different selective and differential media to aid in isolating spoilage yeasts belonging to the Brettanomyces/Dekkera genera. Growth media containing selective and differential factors were employed. These were inoculated with strains of yeast representing Spanish oenological microbiota. Lastly, some of these isolation media were successfully applied in 24 types of wine with a high ethylphenol content, all of which were from the Haro Oenological Station (La Rioja, Spain). p-coumaric acid was determined using High performance liquid chromatography-photodiode-array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS); 4-ethylphenol by using Solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GC-MS); and the rest of the analysis was carried out using official OIV methodology. Actidione is the most effective selective factor for isolating Brettanomyces/Dekkera yeast genera. Other secondary selective factors (selective carbon sources, sorbic acid and ethanol as a microbicide agent) may be used successfully to eliminate potential false positivities; however, they slow growth and delay the time to obtain results.

Cómic, arquitectura narrativa. Describiendo cuatro dimensiones con dos

Cómic, arquitectura narrativa. Describiendo cuatro dimensiones con dos. La mayor parte de los estudios con un enfoque académico en torno a cómic y arquitectura, se han centrado más en las distintas realidades representadas dentro del medio, en sus decorados, que en la estructura del cómic en sí. Existe un aspecto gráfico que se ha venido obviando o al menos no ha adquirido el protagonismo que merece: los espacios representados en el cómic se encuentran en un medio que se concibe y estructura de una manera similar a como el arquitecto dibuja y concibe la arquitectura. En lo básico, históricamente, las herramientas y soportes para idear y representar la arquitectura son muy parecidas a aquellas que usa el autor de cómic. Lápiz, tinta y color sobre un papel, combinadas trabajando en un tablero, ese es lugar de nacimiento de ambos mundos. A lo largo de esta tesis se muestra cómo el dibujo une a creadores de narraciones gráficas y edificios. La relación va más allá de que el cómic pueda utilizar las disposiciones vitruvianas, ichnographia, ortographia o scenographia, (planta, alzado, perspectiva), la propia articulación que hace del espacio de la página, puede sintonizar con la manera en la que se concibe una estructura arquitectónica. La tesis inicia con un capítulo de recorrido en torno a las teorías del cómic existentes, buscando intersecciones posibles con la representación arquitectónica o el proyecto espacial. En este primer paso de estudio del estado de la cuestión han pesado más los trabajos de los últimos veinticinco años. Se trata de un periodo en el que, a la par que se ha producido una explosión creativa en el mundo del cómic, determinados teóricos han incidido en un análisis más formal y visual del medio. Tras recoger teorías en el primer capítulo, se plantea un recorrido histórico a la búsqueda de posibles resonancias de “arquitecturas mentales” en el pasado. Esta búsqueda de referentes más allá del siglo XIX, momento en el que nace el medio en su forma actual, es ya un aspecto comúb en distintos estudios de cómic que han servido de inspiración. Se ha preferido ubicar esta colección de antecedentes como segundo capítulo, evitando dar al trabajo un carácter de historia e incidiendo en su enfoque de tipo visual. El tercer capítulo analiza una serie de obras y autores de cómic en su forma actual, buscando distintas estrategias para la descripción de espacios y las narraciones que ocurren en ellos. El crecimiento de casos en estas últimas décadas ha demostrado hasta qué punto el desarrollo de un campo depende tanto de que exista una masa crítica en un contexto conectado y productivo, como de la aparición de individuos que abren puertas a los demás, con la cristalización personal que hacen de una situación. Se analiza en un cuarto capítulo la relación entre la arquitectura y el cómic modernos. Una relación que se presenta partiendo desde sus respectivos padres, para después tratar un elenco creciente de experiencias en el que la narración gráfica y el hecho arquitectónico estan íntimamente ligados. Inspirado en la estrategia que Gubern y Gasca utilizan en El discurso del cómic (Cátedra, 1988) se cierra la tesis con un discurso visual por las arquitecturas mentales y narrativas del cómic: la representación del espacio a través de juego con las dimensiones, el uso de recursos gráficos propios de la descripción arquitectónica, la sección, la planta, la utilización de elementos constructivos,etc, completando el abanico de estrategias gráficas presentadas. ABSTRACT Comic narrative architecture. Describing four dimensions with two Most of the studies with an academic focus around comics and architecture have centered their view more on the different realities represented in the media, in its sets, than in the structure of comics itself. There is a graphic aspect that has been ignored, or at least has not gained the prominence it deserves: the spaces rendered in the comics are set in a medium that is conceived and structured in a similar way as the architect draws and conceives architecture. In basics, tools for designing and representing architecture are very similar to those used by the cartoonist. Pencil, ink and color on paper, combined working on a board, that is birthplace of both worlds. Throughout this thesis its shown how drawing connects creators of graphic stories and buildings. The relationship goes beyond the use that comics can do of the Vitruvian provisions, ichnographia, ortographia or scenographia (plan, elevation, perspective), to the arrange itself that it makes with space on the page, in tune with the way in which architectural structures are conceived. The thesis starts with a chapter that collects existing comics theories and their possible intersection with architectural rendering. In this first step of study on the state of affairs, the focus has been over studies from the last twenty years. It is a period in which, at the same time that there has been a creative explosion in the world of comics, some theorists have worked with a more formal and visual approach. After collecting theories in the first chapter, a historical tour is made to search for possible resonances of “mental architectures” in the past. This search for references beyond the nineteenth century, when the medium was born in its current form, is already a regular process in various recent studies that have served as inspiration. We preferred to locate this as a second chapter, to avoid giving the work a character of History, and reenforcing its visual approach. The third chapter analyzes a series of comics and authors, looking for different strategies for describing spaces and stories that occur in them. The growth of cases in recent decades has shown how the development of a field depends both on the existence of a critical mass in a connected and productive enviroment, and the appearance of individuals who open doors to others with the personal crystallization that they make on that context. The relationship between architecture and the modern comic is analyzed in a fourth chapter. A relationship that is described starting from their parents, and then going through a growing cast of experiences in which graphic narrative and architecture are closely linked. Inspired by the strategy used Gubern and Gasca in El discurso del cómic (Cátedra, 1988), The Comics’ Speech, the thesis concludes with a visual discourse through this narrative comic architectures: the representation of space by playing with dimensions, the use of resources for architectural rendering, section, plant, etc., to complete the range of graphical strategies presented.

Discourses and values underpin public debate on fracking in Spain: a case study at the crossroad

In the EU context extraction of shale and oil gas by hydraulic fracturing (fracking) differs from country to country in terms of legislation and implementation. While fossil fuel extraction using this technology is currently taking place in the UK, Germany and France have adopted respective moratoria. In between is the Spanish case, where hydrocarbon extraction projects through fracking have to undergo mandatory and routine environmental assessment in accordance with the last changes to environmental regulations. Nowadays Spain is at the crossroad with respect to the future of this technology. We presume a social conflictt in our country since the position and strategy of the involved and confronted social actors -national, regional and local authorities, energy companies, scientists, NGO and other social organization- are going to play key and likely divergent roles in its industrial implementation and public acceptance. In order to improve knowledge on how to address these controverted situations from the own engineering context, the affiliated units from the Higher Technical School of Mines and Energy Engineering at UPM have been working on a transversal program to teach values and ethics. Over the past seven years, this pioneering experience has shown the usefulness of applying a consequentialist ethics, based on a case-by-case approach and costs-benefits analysis both for action and inaction. As a result of this initiative a theoretical concept has arisen and crystallized in this field: it is named Inter-ethics. This theoretical perspective can be very helpful in complex situations, with multi-stakeholders and plurality of interests, when ethical management requires the interaction between the respective ethics of each group; professional ethics of a single group is not enough. Under this inter-ethics theoretical framework and applying content analysis techniques, this paper explores the articulation of the discourse in favour and against fracking technology and its underlying values as manifested in the Spanish traditional mass media and emerging social media such as Youtube. Results show that Spanish public discourse on fracking technology includes the costs-benefits analysis to communicate how natural resources from local communities may be affected by these facilities due to environmental, health and economic consequences. Furthermore, this technology is represented as a solution to the "demand of energy" according to the optimistic discourse while, from a pessimistic view, fracking is often framed as a source "environmental problems" and even natural disasters as possible earthquakes. In this latter case, this negative representation could have been influenced by the closure of a macro project to store injected natural gas in the Mediterranean Sea using the old facilities of an oil exploitation in Amposta (Proyecto Cástor). The closure of this project was due to the occurrence of earthquakes whose intensity was higher than the originally expected by the experts in the assessment stage of the project.

INFORMAR E ENTRETER: O PROCESSO DE PRODUÇÃO DA NOTÍCIA NO HOJE EM DIA

Sabe-se que o entretenimento está incorporado no cotidiano da sociedade moderna de maneira que ultrapassa apenas os momentos de lazer e ócio, fazendo-se presente nos ambientes profissionais, na educação e na informação. Em meio a essa nova concepção, a comunicação também percebe alterações, seja pela interatividade promovida pela midiatização, pelo novo aspecto da audiência, mais exigente e conectada, ou ainda pela hibridização de gêneros e formatos. Neste contexto, surgem programas de entretenimento, especialmente televisivos, que incorporam aspectos técnicos e práticos do jornalismo, incluindo a narrativa e os processos produtivos. Tais programas veiculam um conteúdo denominado de infotenimento, que mescla informação e entretenimento e, assim, a representação jornalística da vida social fica vinculada à distração e ao divertimento. A dissertação faz uma análise do processo de produção do conteúdo noticioso dentro de um programa de entretenimento e variedades que apresenta grande quantidade de conteúdo jornalístico em sua composição: o Hoje Em Dia, da Rede Record. Trata-se de um estudo qualitativo, do tipo estudo de caso, com uso de técnicas de observação direta, entrevistas semiabertas, pesquisa documental e análise de conteúdo. A questão da reprodutibilidade técnica de padrões e a inserção de elementos cotidianos aliados à indústria cultural e à comunicação em massa também é retratada nesta dissertação. A principal intenção é entender como é a dinâmica da produção jornalística em programas que visam entreter e, ao mesmo tempo, informar. De que maneira esses produtos híbridos da modernidade são pensados e como a teoria do infotenimento pode ser aliada às mediações comunicacionais da cultura.

Genetically Modified Food in the US and the UK: proponents, dissenters and media coverage

This research is a qualitative study examining the communication surrounding the issue of genetically modified food in the UK and the UK from October, 2011 through September, 2012. Material from biotechnology industry organizations, industry-funded non-profits, groups campaigning against the continued use of the technology, and mainstream media coverage of the issue in both countries during this time was examined using thematic analysis. The issue is analyzed through the lenses of Herman and Chomsky's propaganda model, agenda building and framing theory. The research finds support for agenda building as well as a modernized understanding of the propaganda model, which the researcher argues are complementary theories.

#DressCodePM & Re-Framing The Niqab: News Sources, Hashtag Activism, and Media Representation

This thesis originates from my interest in exploring how minorities are using social media to talk back to mainstream media. This study examines whether hashtags that trend on Twitter may impact how news stories related to minorities are covered in Canadian media. The Canadian Prime Minister Stephen Harper stated the niqab was “rooted in a culture that is anti-women” on 10 March 2015. The next day #DressCodePM trended in response to the PM’s niqab remarks. Using network gatekeeping theory, this study examines the types of sources quoted in the media stories published on 10 and 11 March 2015. The study’s goal is to explore whether using tweet quotes leads to the representation of a more diverse range of news sources. The study compares the types of sources quoted in stories that covered Harper’s comments without mentioning #DressCodePM versus stories that mention #DressCodePM. This study also uses Tuen A. van Dijk’s methodology of asking “who is speaking, how often and how prominently?” in order to examine whose voices have been privileged and whose voices have been marginalized in covering the niqab in Canadian media from the 1970s and until the days following the PM’s remarks. Network gatekeeping theory is applied in this study to assess whether the gated gained more power after #DressCodePM trended. The case study’s findings indicates that Caucasian male politicians were predominantly used as news sources in covering stories related to the niqab for the past 38 years in the Globe and Mail. The sourcing pattern of favouring politicians continued in Canadian print and online media on 10 March 2015 following Harper’s niqab comments. However, ordinary Canadian women, including Muslim women, were used more often than politicians as news sources in the stories about #DressCodePM that were published on 11 March 2015. The gated media users were able to gain power and attract Canadian Media’s attention by widely spreading #DressCodePM. This study draws attention to the lack of diversity of sources used in Canadian political news stories, yet this study also shows it is possible for the gated media users to amplify their voices through hashtag activism.

Mutual perceptions of Asia and the EU/Europe. Media, elite and public opinion in Singapore and Germany. EU Centre in Singapore Working Paper No. 12, December 2012

In an increasingly interlinked and interdependent world, Europe and Asia are key players. Free trade agreements (FTAs), such as the ones the EU concluded with South Korea and Singapore, are indicative of strong mutual economic interests. It is therefore timely to take a closer look at the mutual perceptions of Asians and Europeans – not only at the governmental and policymaking levels, but also in terms of public opinion and the media. Drawing on data from an extensive research project led by the National Centre for Research on Europe (NCRE), New Zealand, the empirical study in this paper assesses the mutual perceptions of the EU/Europe and Asia, and their respective actors, focusing on two countries – Germany and Singapore. It seeks to do so through an analysis of the data collected from print and broadcast media, interviews with media practitioners, and the findings from public opinion surveys.

Iceland’s contested European Policy: The Footprint of the Past - A Small and Insular Society. Jean Monnet Occasional Paper 01/2013

Introduction. Iceland’s domestic politics and foreign affairs are undergoing drastic changes. After an economic crash, violent protests on the streets of Reykjavik for the first time in Iceland’s history contributed to the defeat of the government. The party system has been altered. A turn has been taken towards Europe after the United States left the island, first by closing its military base in 2006 and then by its clear stance not to assist the country in its economic difficulties. The former close relations with the superpower are unlikely ever to be restored. The EU membership application is placing severe constraints on political parties which are split on the issue and has put in jeopardy the unity of the first left majority in the Icelandic parliament, the Althingi. Society is in a state of flux after an unprecedented economic downscaling and the collapse of almost its entire financial sector – which had boomed rapidly beginning in the mid-1990s. The credibility of politicians, the parliament and the media is in ruins. Iceland’s smallness and its location on the geographical map – one could also say the geopolitical map – has had a profound influence on its domestic and foreign affairs. Iceland is closely associated with the other Nordic states and has adopted many of their domestic characteristics, with important exceptions. On the other hand, the country has come under American influence – geographically, it straddles the Mid-Atlantic rift – and has limited its participation in the European project. Its geographical location in the middle of the North Atlantic has led to a notion that the country’s culture is unique and should be protected by all available means. Politicians continue to play the ‘nationalistic uniqueness’ card with considerable success even though the country has been swept by globalization. Rapid modernization (which only really began in the Second World War with British and American occupations) and sudden engagement with the outside world (which only extended to the general public in the last quarter of the twentieth century) are still slowly but steadily making their mark on the country’s foreign policy. The country’s political discourse and foreign policy still bear the hallmark of the past, i.e. of a small and insular society This paper will address the political developments in Iceland since the 2008 economic crash and place it in a historical context. The aim is to understand Iceland’s present foreign policy and, in particular, the highly contested decision by its government in 2009 to apply for membership of the European Union. The paper is divided into five sections in addition to this introduction and the concluding remarks. First, it starts by explaining the importance in Iceland of a political discourse based on the concept of independence which dates back to the historical narrative of the settlement period. This section will also examine Iceland’s close relations with the other Nordic states – despite important differences between it and the others. Second, the paper will analyse the importance of the party system, i.e. the dominance of the centre-right in Icelandic politics, and the changed nature of the system. Third, it examines how Iceland further distinguishes itself from the other Nordic states in many important features. Fourthly, the paper analyses the country’s three main foreign policy priorities in the post-war period, i.e. extensions of the Exclusive Economic Zone, firm defence arrangements with the US and membership of NATO, and the drive for better market access for marine products – including a partial engagement in the European project. Fifthly, the paper examines how the country’s smallness, in terms of its central administrative capacity, has affected its domestic and foreign policy-making. The concluding section summarizes the main findings concerning the political and historical obstacles that the Social Democratic Alliance faces in its hard-fought battle to change the country’s European Policy.

The press and freedom of speech in Ukraine ahead of parliamentary elections. OSW Commentary No. 90, 2012-09-20

The independence of the mass media has been regularly restricted over the past two years in Ukraine. Following a period of relative freedom in 2005–2010, the scope of direct and indirect government control of the press has increased, cancelling out the achievements of the Orange Revolution in this area. The press in Ukraine is less and less able to perform its role as watchdog on the government and politicians and as a reliable source of information on the situation in the country to the public. This is mainly due to: (1) the concentration of the most important mass media in the hands of Ukraine’s most powerful oligarchs, whose business interests depend on the government; (2) the use of the press as instruments in political and business competition; (3) the ruling class’s subordination of the institutions which supervise the press; (4) repression used against media critical of the government and (5) the lack of an independent public broadcasting corporation. As a consequence, the press has hardly any impact on the political processes taking place ahead of the parliamentary election scheduled for 28 October. This is also an effect of a passiveness present in the Ukrainian public, who are tired of politics and are focused on social issues. Cases of abuse or corruption scandals revealed by the press do not provoke any response from the public and are rarely investigated by the public prosecution authorities. The more popular a given medium is, the more strongly it is controlled by the government. At present, television has to be recognised as the least reliable of the mass media. In turn, Internet news journals are characterised by the greatest pluralism but also have more limited accessibility. The political conditions in which the mass media operate in Ukraine lead to various forms of pathology. The most serious of them are censorship by the owners and self-censorship performed by journalists, and a great share of political advertorials. As the parliamentary election is approaching, the pathologies of the Ukrainian media market have been showing up with greater intensity.

Occupational employment in transportation, communications, utilities, and trade.

"April 1982."

Development of materials and public relations efforts to promote child passenger safety. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Baseline data for public education about alcohol and highway safety in Vermont.

National Highway Traffic Safety Administration, Washington, D.C.

Handbook for a field research program for driver's license law enforcement. User guidelines and handbook.

National Highway Traffic Safety Administration, Washington, D.C.

Responses of women and men to traffic safety messages: a qualitative report.

National Highway Traffic Safety Administration, Washington, D.C.

ASAP public information and education. Analytic study number seven. Final report.

National Highway Traffic Safety Administration, Washington, D.C.