Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)

In the present study, the quality of post-thaw sperm of red seabream Pagrus major frozen with 6-24% DMSO was investigated. The motility, average path velocity and fertilizing capacity of fresh and their corresponding post-thaw sperm were examined for evaluation of the post-thaw sperm motion characteristics and its association with fertilizing capacity. An analysis of sperm motility before and after cryopreservation has been performed using computer-assisted sperm analysis (CASA). For post-thaw sperm frozen with 12-21% DMSO, the percentages of motile sperm were not significantly (P > 0.05) changed 10 s after activation. Moreover, the main motility pattern and swimming velocity of the motile post-thaw sperm were not significantly (P > 0.05) changed and the progressive linear motion was still the dominant pattern. However, the total motility of post-thaw sperm (72.3 +/- 6.3%) 30 s after activation was (P < 0.05) lower than the corresponding fresh sperm (82.7 +/- 7.2%). Additionally, the fertilizing capacity of post-thaw sperm was investigated with a standardized sperm to egg ratio 500:1. There is a linear regression relationship between the percentage of motile post-thaw sperm and fertilizing capability. These data demonstrate that 12-21% DMSO can provide good protection to the sperm during the freezing-thawing process. (c) 2006 Elsevier B.V. All rights reserved.

Hora da colheita: hora de cuidar do seu produto e de você. Casa de embalagem de lona.

A publicação apresenta uma estrutura móvel construída com lona e tubo de ferro que pode ser usada como uma casa de embalagem rudimentar, onde as hortaliças são armazenadas e preparadas entre a colheita e a expedição.

Avaliação da reação de genótipos de soja ao cancro da haste, em casa de vegetação.

Identificar genótipos resistentes ao cancro da haste, causado por Diaporthe phaseolorum f. sp. meridionalis, visando o melhoramento genético de soja (Glycine max) e o desenvolvimento de cultivares resistentes.

Plan de marketing de "Casa Rural Aramendía"

De acuerdo a la normativa de TFEs el repositorio no puede dar acceso a este trabajo. Para consultarlo póngase en contacto con el tutor del trabajo. Puede acceder al resumen del mismo pinchando en el pdf adjunto

Clarin sonoro, que haze gente para el cielo, o Tratado de las penas, qve padecen los condenados

Signaturas: [¶]4, A-Q8, R4.