The 66 k-Da protein identified as a light meromyosin is involved in the setting of surimi

The 66 kilo-Dalton (k-Da) protein split off from the cross linked myosin heavy chain (CMHC) formed due to the setting of Alaska pollack surimi, frozen-storage of Pacific cod flesh, and vinegar-curing of Pacific mackerel mince was identified as a light meromyosin (LMM). Puncture and stress-relaxation tests showed that the actomyosin subunits (AMS) of Alaska pollack surimi, upon setting at 30°C, transformed into gel, although the elasticity of this gel was very low when compared to the gels from surimi or actomyosin (AM). Electrophoretic studies showed that the band due to LMM in the gel from AMS gradually disappeared with the progress of setting but higher molecular weight polymer did not form. The intensity of the bands due to other myosin sub-fragments decreased a little. The findings suggest that at setting temperature, LMM of MHC molecule leads to an unfolding resulting in an intramolecular aggregation through non-covalent interactions, and thus plays a significant role in the crosslinking of MHC.

Rearing of Labeo bata in sewage-fed fish culture pond

Labeo bata is a highly priced fish which commanding good consumer preference, even if the size is very small (15 to 20 g each), especially in West Bengal. With a view to evaluating the culture potential of this fish, repeated experiments were conducted at the Wastewater Aquaculture Division of the Central Institute of Freshwater Aquaculture, Rahara, West Bengal, during 1991-93 in ponds fed with treated domestic sewage effluent. An average production of 1270.63 kg per ha was obtained within 6 to 10 months for a uniform stocking density of 50,000 seed per ha. The average size attained by the fish in the different sets of experiments varied from 23.3 to 37.9 g as against the initial weight of 0.096 to 0.193 g. Details of management, growth, survival, quantity of sewage used and pond nutrient status and productivity are dealt with in this communication.