Horizontal genetic transfer in asexual fungi

Four aspects of horizontal genetic transfer during heterokaryon formation were examined in the asexual pathogen Fusarium oxysporum f.sp. cubense (Foc): (1) variability based on method of heterokaryon formation; (2) differences in nuclear and mitochondrial inheritance; (3) the occurrence of recombination without nuclear fusion; (4) the occurrence of horizontal genetic transfer between distantly related isolates. The use of non-pathogenic strains of Fusarium oxysporum as biocontrol agents warrants a closer examination at the reproductive life cycle of this fungus, particularly if drug resistance or pathogenicity genes can be transmitted horizontally. Experiments were divided into three phases. Phase I looked at heterokaryon formation by hyphal anastomosis and protoplast fusion. Phase II was a time course of heterokaryon formation to look at patterns of nuclear and mitochondrial inheritance. Phase III examined the genetic relatedness of the different vegetative compatibility groups using a multilocus analysis approach. Heterokaryon formation was evident within and between vegetative compatibility groups. Observation of non-parental genotypes after heterokaryon formation confirmed that, although a rare event, horizontal genetic transfer occurred during heterokaryon formation. Uniparental mitochondria inheritance was observed in heterokaryons formed either by hyphal anastomosis or protoplast fusion. Drug resistance was expressed during heterokaryon formation, even across greater genetic distances than those distances imposed by vegetative compatibility. Phylogenies inferred from different molecular markers were incongruent at a significant level, challenging the clonal origins of Foc. Mating type genes were identified in this asexual pathogen Polymorphisms were detected within a Vegetative Compatibility Group (VCG) suggesting non-clonal inheritance and/or sexual recombination in Foc. This research was funded in part by a NIH-NIGMS (National Institutes of Health-National Institute of General Medical Sciences) Grant through the MBRS (Minority Biomedical Research Support), the Department of Biological Sciences and the Tropical Biology Program at FIU. ^

Determination of vertical and horizontal pathways of injected fresh wastewater into a deep saline aquifer (Florida, USA) using natural chemical tracers

Two deep-well injection sites in south Florida, USA, inject an average of 430 million liters per day (MLD) of treated domestic fresh wastewater into a deep saline aquifer 900 m below land surface. Elevated levels of NH3 (highest concentration 939 µmol) in the overlying aquifer above ambient concentrations (concentration less than 30 µmol) were evidence of the upward migration of injected fluids. Three pathways were distinguished based on ammonium, chloride and bromide ratios, and temperature. At the South District Wastewater Treatment Plant, the tracer ratios showed that the injectate remained chemically distinct as it migrated upwards through rapid vertical pathways via density-driven buoyancy. The warmer injectate (mean 28°C) retained the temperature signal as it vertically migrated upwards; however, the temperature signal did not persist as the injectate moved horizontally into the overlying aquifers. Once introduced, the injectate moved slowly horizontally through the aquifer and mixed with ambient water. At the North District Wastewater Treatment Plant, data provide strong evidence of a one-time pulse of injectate into the overlying aquifers due to improper well construction. No evidence of rapid vertical pathways was observed at the North District Wastewater Treatment Plant.

Developing an enhanced model for combined heat and air infiltration energy simulation

The need for efficient, sustainable, and planned utilization of resources is ever more critical. In the U.S. alone, buildings consume 34.8 Quadrillion (1015) BTU of energy annually at a cost of $1.4 Trillion. Of this energy 58% is utilized for heating and air conditioning. ^ Several building energy analysis tools have been developed to assess energy demands and lifecycle energy costs in buildings. Such analyses are also essential for an efficient HVAC design that overcomes the pitfalls of an under/over-designed system. DOE-2 is among the most widely known full building energy analysis models. It also constitutes the simulation engine of other prominent software such as eQUEST, EnergyPro, PowerDOE. Therefore, it is essential that DOE-2 energy simulations be characterized by high accuracy. ^ Infiltration is an uncontrolled process through which outside air leaks into a building. Studies have estimated infiltration to account for up to 50% of a building's energy demand. This, considered alongside the annual cost of buildings energy consumption, reveals the costs of air infiltration. It also stresses the need that prominent building energy simulation engines accurately account for its impact. ^ In this research the relative accuracy of current air infiltration calculation methods is evaluated against an intricate Multiphysics Hygrothermal CFD building envelope analysis. The full-scale CFD analysis is based on a meticulous representation of cracking in building envelopes and on real-life conditions. The research found that even the most advanced current infiltration methods, including in DOE-2, are at up to 96.13% relative error versus CFD analysis. ^ An Enhanced Model for Combined Heat and Air Infiltration Simulation was developed. The model resulted in 91.6% improvement in relative accuracy over current models. It reduces error versus CFD analysis to less than 4.5% while requiring less than 1% of the time required for such a complex hygrothermal analysis. The algorithm used in our model was demonstrated to be easy to integrate into DOE-2 and other engines as a standalone method for evaluating infiltration heat loads. This will vastly increase the accuracy of such simulation engines while maintaining their speed and ease of use characteristics that make them very widely used in building design.^

O desempenho da compartimentação horizontal seletiva na promoção da segurança contra incêndio em edificações

The thesis presents the results of research that addresses the performance of selective horizontal partitioning to promote Fire Safety in Buildings - FSB. Horizontal partitioning is a passive protection measure, settable in the early stages of the design process and controlled by the architect. However, there is a frequent reconfiguration of the rooms in academic buildings to adjust them for the space demand. Thus, large classrooms could turn into two or more smaller rooms, for example. Regardless when the subdivision occurs in the design phase or during the occupation of the building, the regulations just ensures the compartimentation of the room if all fireguard devices are present in the room. Knowing the fire's first minutes are the most important for life protection, we defend the hypothesis that a kind of partitioning ignored by regulatory standards is able to favoring the building vacancy and occupants rescue, for promote the room’s smoke exhaust. The performance of the selective horizontal partitioning due different blend of openings for smoke outlet was simulated on CFD software. The results indicate that selective horizontal partitioning is able to promote an upper smoke free layer and delay the indoor temperature growth.

O desempenho da compartimentação horizontal seletiva na promoção da segurança contra incêndio em edificações

The thesis presents the results of research that addresses the performance of selective horizontal partitioning to promote Fire Safety in Buildings - FSB. Horizontal partitioning is a passive protection measure, settable in the early stages of the design process and controlled by the architect. However, there is a frequent reconfiguration of the rooms in academic buildings to adjust them for the space demand. Thus, large classrooms could turn into two or more smaller rooms, for example. Regardless when the subdivision occurs in the design phase or during the occupation of the building, the regulations just ensures the compartimentation of the room if all fireguard devices are present in the room. Knowing the fire's first minutes are the most important for life protection, we defend the hypothesis that a kind of partitioning ignored by regulatory standards is able to favoring the building vacancy and occupants rescue, for promote the room’s smoke exhaust. The performance of the selective horizontal partitioning due different blend of openings for smoke outlet was simulated on CFD software. The results indicate that selective horizontal partitioning is able to promote an upper smoke free layer and delay the indoor temperature growth.

Coseismic horizontal slip revealed by sheared clastic dikes in the Dead Sea Basin

Peer reviewed

Micro-infiltration et rétention des scellants : évaluation de trois protocoles de mise en place

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

Micro-infiltration et rétention des scellants : évaluation de trois protocoles de mise en place

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

Impulsão horizontal de futebolistas da segunda divisão do campeonato gaúcho

No futebol a impulsao tem impacto no cabeceio, nos movimentos de antecipaçoes, nos diferentes saltos e de forma geral, na velocidade das açoes de jogo. No entanto, ao contrário de outros esportes como Basquete, Vôlei, Handebol, o seu treinamento específico é pouco visado no futebol. O objetivo deste estudo é verificar, em atletas profissionais de futebol de campo, o perfil da impulsao horizontal e o desenvolvimento dessa durante uma temporada de treinamento e jogos pela segunda divisao do campeonato gaúcho. Foram avaliados 18 atletas de futebol (com média de idade 25±5,44 anos, altura 177,5±6,48 cm e massa corporal 79,4±9,67 kg). Os atletas se submeteram a testes de impulsao horizontal no início (pré) e após três meses de treinamentos (pós). Mediu-se os saltos com ambas às pernas, cinco saltos consecutivos com membro direito (MID) e esquerdo (MIE) e cinco saltos alternados. O perfil de impulsao obtido no pré-teste foi respectivamente 2,22±0,14m, 11,46±0,56 m, 11,69±0,73m e 12,04±0,78m. Os resultados do pós-teste nao se alteraram em comparaçao ao primeiro teste, sendo respectivamente 2,22±0,15m, 11,26±1,12m, 11,46±0,95m e 12,02±0,76m. Conclui-se que o treinamento nao foi capaz de proporcionar desenvolvimento desta capacidade, pois predominantemente as rotinas de treinamento de futebol nao enfocam este desenvolvimento

Impulsão horizontal de futebolistas da segunda divisão do campeonato gaúcho

No futebol a impulsao tem impacto no cabeceio, nos movimentos de antecipaçoes, nos diferentes saltos e de forma geral, na velocidade das açoes de jogo. No entanto, ao contrário de outros esportes como Basquete, Vôlei, Handebol, o seu treinamento específico é pouco visado no futebol. O objetivo deste estudo é verificar, em atletas profissionais de futebol de campo, o perfil da impulsao horizontal e o desenvolvimento dessa durante uma temporada de treinamento e jogos pela segunda divisao do campeonato gaúcho. Foram avaliados 18 atletas de futebol (com média de idade 25±5,44 anos, altura 177,5±6,48 cm e massa corporal 79,4±9,67 kg). Os atletas se submeteram a testes de impulsao horizontal no início (pré) e após três meses de treinamentos (pós). Mediu-se os saltos com ambas às pernas, cinco saltos consecutivos com membro direito (MID) e esquerdo (MIE) e cinco saltos alternados. O perfil de impulsao obtido no pré-teste foi respectivamente 2,22±0,14m, 11,46±0,56 m, 11,69±0,73m e 12,04±0,78m. Os resultados do pós-teste nao se alteraram em comparaçao ao primeiro teste, sendo respectivamente 2,22±0,15m, 11,26±1,12m, 11,46±0,95m e 12,02±0,76m. Conclui-se que o treinamento nao foi capaz de proporcionar desenvolvimento desta capacidade, pois predominantemente as rotinas de treinamento de futebol nao enfocam este desenvolvimento

Continuous VM-ADCP (vessel-mounted acoustic Doppler current profiler) profiles of horizontal velocities and raw acoustic gain control data during Polarstern cruise ANT-XVIII/2

The mixing regime of the upper 180 m of a mesoscale eddy in the vicinity of the Antarctic Polar Front at 47° S and 21° E was investigated during the R.V. Polarstern cruise ANT-XVIII/2 within the scope of the iron fertilization experiment EisenEx. On the basis of hydrographic CTD and ADCP profiles we deduced the vertical diffusivity Kz from two different parameterizations. Since these parameterizations bear the character of empirical functions, based on theoretical and idealized assumptions, they were inter alia compared with Cox-number and Thorpe-scale related diffusivities deduced from microstructure measurements, which supplied the first direct insights into turbulence of this ocean region. Values of Kz in the range of 10**-4 - 10**-3 m**2/s appear as a rather robust estimate of vertical diffusivity within the seasonal pycnocline. Values in the mixed layer above are more variable in time and reach 10**-1 m**2/s during periods of strong winds. The results confirm a close agreement between the microstructure-based eddy diffusivities and eddy diffusivities calculated after the parameterization of Pacanowski and Philander [1981, Journal of Physical Oceanography 11, 1443-1451, doi:10.1175/1520-0485(1981)011<1443:POVMIN>2.0.CO;2].

ROCK OUTCROPS IN THE CANADIAN SHIELD: AN INVESTIGATION OF CONTAMINANT TRANSPORT FROM SURFACE SOURCES IN FRACTURED ROCK AQUIFERS

Flow, recharge and transport dynamics in fractured rock aquifers with low lying rock outcrops is a largely unexplored area of study in hydrogeology. The purpose of this thesis is to examine these topics in an agricultural area in Eastern Ontario. The study consists of a regional scale groundwater quality study, an infiltration experiment that considers bacteria transport from the ground surface to a well, and a numerical modelling study that tests the parameters that affect surface infiltration of a tracer from a rock outcrop to a deeper horizontal fracture. In the water quality study, approximately 65% of the samples contained total coliform, 16% contained E. coli, and 1% contained nitrate-N at greater than 5 mg/L. Occurrence of E. coli increased when considering seasonality, where wells were drilled on rock outcrops, and for shallow well intervals. Nitrate-N did not occur above the Guidelines for Canadian Drinking Water Quality (Health Canada, 2012) of 10 mg/L. Rapid arrival times were observed in the infiltration study for both the microspheres (30 minutes) and a dye tracer (45 minutes) in a well approximately 6.0 m in horizontal and 2.8 m in vertical distance from the tracer source. Transport velocities were approximately 38.9 m/day for the dye tracer and 115.2 m/day for the colloidal tracer. Results of the model runs indicate that overburden can provide an effective protective layer to transport in fractures, that high groundwater velocities occur in larger fracture apertures and higher gradients dilute tracer concentrations, and that lower groundwater velocities occur with smaller fracture apertures and lower gradients result in elevated tracer concentrations. Lower rainfall rates, larger fracture apertures, early tracer time, larger gradients, and lower water levels maintained unsaturated conditions for longer time periods such that tracer transport was delayed until saturated conditions were attained. The overall heterogeneity of this aquifer environment creates a source water protection conundrum where the water quality is generally good, while transport can occur very quickly in proximity to rock outcrops and in areas with limited overburden.

IL-1α and inflammasome-independent IL-1β promote neutrophil infiltration following alum vaccination

Despite its long record of successful use in human vaccines, the mechanisms underlying the immunomodulatory effects of alum are not fully understood. Alum is a potent inducer of interleukin-1 (IL-1) secretion in vitro in dendritic cells and macrophages via Nucleotide-binding domain and leucine-rich repeat-containing (NLR) family, pyrin domain-containing 3 (NLRP3) inflammasome activation. However, the contribution of IL-1 to alum-induced innate and adaptive immune responses is controversial and the role of IL-1α following alum injection has not been addressed. This study shows that IL-1 is dispensable for alum-induced antibody and CD8 T cell responses to ovalbumin. However, IL-1 is essential for neutrophil infiltration into the injection site, while recruitment of inflammatory monocytes and eosinophils is IL-1 independent. Both IL-1α and IL-1β are released at the site of injection and contribute to the neutrophil response. Surprisingly, these effects are NLRP3-inflammasome independent as is the infiltration of other cell populations. However, while NLRP3 and caspase 1 were dispensable, alum-induced IL-1β at the injection site was dependent on the cysteine protease cathepsin S. Overall, these data demonstrate a previously unreported role for cathepsin S in IL-1β secretion, show that inflammasome formation is dispensable for alum-induced innate immunity and reveal that IL-1α and IL-1β are both necessary for alum-induced neutrophil influx in vivo.