EC27-811 Harvesting Wheat in Nebraska with the Combined Harvester Thresher 1926

Frequent inquiry from wheat growers in Nebraska and others as to the relative merits of the combined harvester-thresher as an efficienct and economical harvesting machine led the Nebraska Agricultural College to make a study of this problem in the summer of 1926. The work was carried on by the Departments of Rural Economics and Agricultural Engineering, cooperating with the United States Department of Agriculture which was conducting a similar survey in different parts of the United States. Perkins county, Nebraska, was the area selected for study as it is more or less typical of those parts of the state where combines are used extensively. The purpose of this circular is merely to give such informaition as is available regarding harvesting costs by different methods in Nebraska and not to recommend one method over another since location, acreage, climatic conditions, and topopgraphy all have their influence in determining the most economic method for different communities.

G. Houghton, Hüftbild, Stillorgan, 1853

Signatur des Originals: S 36/F12242

DNA alignment and Bayesian trees of partial sequences of COI, COIII and the haemocyanin functional unit f-g of 28 octopod species

Background: Octopods have successfully colonised the world's oceans from the tropics to the poles. Yet, successful persistence in these habitats has required adaptations of their advanced physiological apparatus to compensate impaired oxygen supply. Their oxygen transporter haemocyanin plays a major role in cold tolerance and accordingly has undergone functional modifications to sustain oxygen release at sub-zero temperatures. However, it remains unknown how molecular properties evolved to explain the observed functional adaptations. We thus aimed to assess whether natural selection affected molecular and structural properties of haemocyanin that explains temperature adaptation in octopods. Results: Analysis of 239 partial sequences of the haemocyanin functional units (FU) f and g of 28 octopod species of polar, temperate, subtropical and tropical origin revealed natural selection was acting primarily on charge properties of surface residues. Polar octopods contained haemocyanins with higher net surface charge due to decreased glutamic acid content and higher numbers of basic amino acids. Within the analysed partial sequences, positive selection was present at site 2545, positioned between the active copper binding centre and the FU g surface. At this site, methionine was the dominant amino acid in polar octopods and leucine was dominant in tropical octopods. Sites directly involved in oxygen binding or quaternary interactions were highly conserved within the analysed sequence. Conclusions: This study has provided the first insight into molecular and structural mechanisms that have enabled octopods to sustain oxygen supply from polar to tropical conditions. Our findings imply modulation of oxygen binding via charge-charge interaction at the protein surface, which stabilize quaternary interactions among functional units to reduce detrimental effects of high pH on venous oxygen release. Of the observed partial haemocyanin sequence, residue 2545 formed a close link between the FU g surface and the active centre, suggesting a role as allosteric binding site. The prevalence of methionine at this site in polar octopods, implies regulation of oxygen affinity via increased sensitivity to allosteric metal binding. High sequence conservation of sites directly involved in oxygen binding indicates that functional modifications of octopod haemocyanin rather occur via more subtle mechanisms, as observed in this study.

Opinion and decision in the United States of America on behalf of George W. Hopkins, claimant, v. the United Mexican States. Docket no. 39, March 31, 1926.

Mode of access: Internet.

The life of George, fourth earl of Aberdeen, K. G., K. T.,

"A short bibliography of the fourth Earl of Aberdeen, by J. M. Bulloch": vol.II, p. 334-339.

George Fuller, his life and works;

Introductory note signed: Josiah B. Millet.