Variabilidade das sub-populações de espermatozóides avaliados pela cinética em sistema computadorizado e combinação de sondas fluorescentes como praâmetro quantitativo do sêmen congelado de ovinos

Pós-graduação em Medicina Veterinária - FMVZ

Criopreservação e fertilidade de espermatozoides do ejaculado e recuperados da cauda do epidídimo de garanhões subférteis

Pós-graduação em Medicina Veterinária - FMVZ

Indução a espermiação do mandi-pintado, Pimelodus britskii no período reprodutivo (Teleostei: Pimelodidae)

Pós-graduação em Aquicultura - FCAV

Planejamento de cursos online para professores de alemão: parâmetros em mapa conceitual

Distance-learning language courses are gaining ground because they meet contemporary social demands and expand opportunities for access to new knowledge. They also favor interaction among people with similar goals but who live in places where no live learning arrangements are available. Taking Valente (2002), Garrison and Anderson (2000), and other authors for theoretical bases, this paper reflects on the criteria and aspects to be considered when one plans and ministers online teacher training courses. The data were taken from a distance-based university extension course offered on the Moodle learning platform by a university in the State of São Paulo, Brazil. The objective was to encourage German language teachers to further develop their own language skills, reflect on aspects of inter-culturality in teaching the target language, and provide experience with current technological resources. This paper describes the elements that proved to be relevant in the creation and planning of the course, based on authors who deal with characteristics of computer-mediated teaching. The importance of this study is related to the fact that little information is available on the planning of courses in online formats.

Aspectos reprodutivos, morfologia dos gametas e desenvolvimento embrionário de Astyanax altiparanae Garutti & Britski (Teleostei, Characidae)

Pós-graduação em Aquicultura - FCAV

Application of two segmentation protocols during the processing of virtual images in rapid prototyping: ex vivo study with human dry mandibles

The aim of this study was to evaluate the accuracy of virtual three-dimensional (3D) reconstructions of human dry mandibles, produced from two segmentation protocols (outline only and all-boundary lines).Twenty virtual three-dimensional (3D) images were built from computed tomography exam (CT) of 10 dry mandibles, in which linear measurements between anatomical landmarks were obtained and compared to an error probability of 5 %.The results showed no statistically significant difference among the dry mandibles and the virtual 3D reconstructions produced from segmentation protocols tested (p = 0,24).During the designing of a virtual 3D reconstruction, both outline only and all-boundary lines segmentation protocols can be used.Virtual processing of CT images is the most complex stage during the manufacture of the biomodel. Establishing a better protocol during this phase allows the construction of a biomodel with characteristics that are closer to the original anatomical structures. This is essential to ensure a correct preoperative planning and a suitable treatment.

Sperm fertility and viability following 48 h of refrigeration: Evaluation of different extenders for the preservation of bull semen in liquid state

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Temperature and storage period over spermatic parameters of jundia, Rhamdia quelen (Quoy & Gaimard, 1824)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effect of glycerol on the viability and fertility of cooled bovine semen

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of hydrogen peroxide on thawed ovine sperm motility

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of digitization parameters on periapical radiographic image quality with regard to anatomic landmarks

The aim of this study was to evaluate the influence of digitization parameters on periapical radiographic image quality, with regard to anatomic landmarks. Digitized images (n = 160) were obtained using a flatbed scanner with resolutions of 300, 600 and 2400 dpi. The radiographs of 2400 dpi were decreased to 300 and 600 dpi before storage. Digitizations were performed with and without black masking using 8-bit and 16-bit grayscale and saved in TIFF format. Four anatomic landmarks were classified by two observers (very good, good, moderate, regular, poor), in two random sessions. Intraobserver and interobserver agreements were evaluated by Kappa statistics. Inter and intraobserver agreements ranged according to the anatomic landmarks and resolution used. The results obtained demonstrated that the cement enamel junction was the anatomic landmark that presented the poorest concordance. The use of black masking provided better results in the digitized image. The use of a mask to cover radiographs during digitization is necessary. Therefore, the concordance ranged from regular to moderate for the intraobserver evaluation and concordance ranged from regular to poor for interobserver evaluation.

Assessment of CT numbers in limited and medium field-of-view scans taken using Accuitomo 170 and Veraviewepocs 3De cone-beam computed tomography scanners

To assess the influence of anatomic location on the relationship between computed tomography (CT) number and X-ray attenuation in limited and medium field-of-view (FOV) scans. Materials and Methods Tubes containing solutions with different concentrations of K2HPO4 were placed in the tooth sockets of a human head phantom. Cone-beam computed tomography (CBCT) scans were acquired, and CT numbers of the K2HPO4 solutions were measured. The relationship between CT number and K2HPO4 concentration was examined by linear regression analyses. Then, the variation in CT number according to anatomic location was examined. Results The relationship between K2HPO4 concentration and CT number was strongly linear. The slopes of the linear regressions for the limited FOVs were almost 2-fold lower than those for the medium FOVs. The absolute CT number differed between imaging protocols and anatomic locations. Conclusion There is a strong linear relationship between X-ray attenuation and CT number. The specific imaging protocol and anatomic location of the object strongly influence this relationship.

Imageologia na odontologia e aspectos legais

The technology, through the advent of new equipments that allow imaging exams, has helped very much in the diagnosis and prognosis of diseases in Dentistry. The use of informatics, in general, in the manufacture of clinical reports is increasingly present in the dental offices. The legal validity of these systems is questioned, and is matter of discussion. This work makes considerations about Imageology or Diagnostic by image, a new area that is appearing on Dentistry. Among other exams, there are: digital radiography, tomography, computed tomography, artomography, magnetic resonance, computed cefalometry and ultra-sonography. It permits the professional to obtain a better diagnostic, and to the patient, the visualization of his problem and treatment. A survey on the possibilities of using informatics in Dentistry, particularly in Radiology, was also carried out, as well as the legal aspects, which are in accordance with the Law 8.935/94, what guarantees its practicability. Digital Certification is a mechanism that provides legal validity to documents and, as such, to radiographic images and others. It is a procedure that the dentist should take to ensure that he/she has safeguarded the judicial proofs that may be necessary in an eventual demand.

Expression of the classical and nonclassical HLA molecules in breast cancer

Considering that downregulation of HLA expression could represent a potential mechanism for breast carcinogenesis and metastasis, the aim of the present study was to use immunohistochemical methods to analyze the expression of HLA-Ia, HLA-DR, HLA-DQ, HLA-E, and HLA-G in invasive ductal carcinoma (IDC) of the breast and to relate this HLA profile to anatomopathological parameters. Fifty-two IDC from breast biopsies were stratified according to histological differentiation (well, moderately, and poorly differentiated) and to the presence of metastases in axillary lymph nodes. The expression of HLA molecules was assessed by immunohistochemistry, using a computer-assisted system. Overall, 31 (59.6%) out of the 52 IDC breast biopsies exhibited high expression of HLA-G, but only 14 (26.9%) showed high expression of HLA-E. A large number (41, 78.8%) of the biopsies showed low expression of HLA-Ia, while 45 (86.5%) showed high expression of HLA-DQ and 36 (69.2%) underexpressed HLA-DR. Moreover, 24 (41.2%) of 52 biopsies had both low HLA-Ia expression and high HLA-G expression, while 11 (21.2%) had low HLA-Ia expression and high HLA-E expression. These results suggest that, by different mechanisms, the downregulation of HLA-Ia, HLA-E, and HLA-DR and the upregulation of HLA-G and HLA-DQ are associated with immune response evasion and breast cancer aggressiveness.