847 resultados para Communication for Development and Social Change


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An efficient cryopreservation protocol was developed for mature seeds of Oncidium flexuosum Sims. Seed morphology, protocorm formation, and early seedling development were also assessed. The effects of phloroglucinol and Supercool X-1000® as cryoprotectant additives in the vitrification solution were investigated. Dehydration using the plant vitrification solution 2 (PVS2) for 60 and 120 min prior to immersion in liquid nitrogen promoted the highest frequency of in vitro seed germination 6 weeks following culture on half-strength Murashige and Skoog (1/2 MS) medium. Mature seeds submitted to vitrification for 120 min in PVS2 and 1 % phloroglucinol at 0 °C enhanced germination by 68 %, whereas in PVS2 and 1 % Supercool X-1000® germination was just moderately enhanced (26 %). In vitro-germinating seedlings developed healthy shoots and roots without the use of plant growth regulators. After 6 months of growth, there were no differences between in vitro- and ex vitro-grown seedlings for various phenotypic characteristics, including shoot length, number of leaves, number and length of roots, and fresh and dry weight. Seedlings were transferred to greenhouse conditions and successfully acclimatized, further developing into normal plants with over 90 % survival. Comparative analysis of seedlings from control and vitrified seeds using flow cytometry indicated that no change in ploidy levels occurred as a result of cryopreservation, therefore maintaining seedlings genetic stability. In this study, vitrification with PVS2 for 120 min with the addition of 1 % phloroglucinol offers a simple, safe, and feasible protocol for cryopreservation of O. flexuosum mature seeds. © 2013 Springer Science+Business Media Dordrecht.

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Informa de los avances logrados desde el octavo periodo de sesiones del CDCC en las areas de prioridad de cooperacion tecnica entre paises en desarrollo: informacion, ciencia y tecnologia, eliminacion de barreras idiomaticas, planificacion, sector agropecuario, sector social, educacion y cultura, transporte y comunicaciones, comercio, energia y recursos naturales.

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Se refiere a resoluciones que emanan de otros organos de la ONU, incluso la CEPAL, el Consejo Economico y Social y la Asamblea General. El documento pretende llamar a la atencion del Comite las acciones de seguimiento tomadas acerca de ciertos asuntos que el Comite ha considerado en sesiones anteriores y que tengan alguna implicacion directa o indirecta para el CDCC o que sean de interes para el trabajo de la Secretaria.

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Presenta el proyecto del Programa de Trabajo 1988-1989 del CDCC, en que las principales areas de actividad expresadas como elementos de programa son: a) estudio economico de paises del Caribe; b) agricultura y alimentacion; c) planificacion economica y social; d) ciencia y tecnologia; e) recursos naturales, gestion ambiental y desarrollo; f) informacion y documentacion para el desarrollo economico y social; g) apoyo de la CEPAL a los paises del Caribe Oriental; h) banco de datos estadisticos; i) comercio y finanzas internacionales; j) transporte y comunicaciones; y k) poblacion.