La casa crecedera. El crecimiento programado de la vivienda con innovación europea y economía de medios latinoamericana

El concepto de casa crecedera, tal y como lo conocemos en la actualidad, se acuñó por primera vez en 1932 en el concurso Das Wachsende Haus organizado por Martin Wagner y Hans Poelzig dentro del marco de la Exposición Internacional Sonne, Luft und Haus für alle, promovida por la Oficina de Turismo de la ciudad de Berlín. En dicho concurso, se definía este tipo de vivienda como aquella célula básica o vivienda semilla que, dependiendo de las necesidades y posibilidades de los habitantes, podía crecer mediante otras estancias, conformando una vivienda completa en sí misma en cada fase de crecimiento. Numerosos arquitectos de primer orden, tales como Walter Gropius, Bruno Taut, Erich Mendelsohn o Hans Scharoun, participaron en este concurso, abriendo una nueva vía de exploración dentro de la vivienda flexible, la del crecimiento programado. A partir de ese momento, en Europa, y subsecuentemente en EEUU y otras regiones desarrolladas, se iniciaron numerosas investigaciones teóricas y prácticas en torno al fenómeno del crecimiento en la vivienda desde un enfoque vinculado a la innovación, tanto espacial como técnica. Por otro lado, aunque dentro del marco de la arquitectura popular de otros países, ya se ensayaban viviendas crecederas desde el siglo XVIII debido a que, por su tamaño, eran más asequibles dentro del mercado. Desde los años treinta, numerosos países en vías de desarrollo tuvieron que lidiar con migraciones masivas del campo a la ciudad, por lo que se construyeron grandes conjuntos habitacionales que, en numerosos casos, estaban conformados por viviendas crecederas. En todos ellos, la aproximación al crecimiento de la vivienda se daba desde una perspectiva diferente a la de los países desarrollados. Se primaba la economía de medios, el uso de sistemas constructivos de bajo costo y, en muchos casos, se fomentaba incluso la autoconstrucción guiada, frente a las construcciones prefabricadas ensambladas por técnicos especializados que se proponían, por ejemplo, en los casos europeos. Para realizar esta investigación, se recopiló información de estas y otras viviendas. A continuación, se identificaron distintas maneras de producir el crecimiento, atendiendo a su posición relativa respecto de la vivienda semilla, a las que se denominó mecanismos de ampliación, utilizados indistintamente sin tener en cuenta la ubicación geográfica de cada casa. La cuestión de porqué se prefiere un mecanismo en lugar de otro en un caso determinado, desencadenó el principal objetivo de esta Tesis: la elaboración de un sistema de análisis y diagnóstico de la vivienda crecedera que, de acuerdo a determinados parámetros, permitiera indicar cuál es la ampliación o sucesión de ampliaciones óptimas para una familia concreta, en una ubicación establecida. Se partió de la idea de que el crecimiento de la vivienda está estrechamente ligado a la evolución de la unidad de convivencia que reside en ella, de manera que la casa se transformó en un hábitat dinámico. Además se atendió a la complejidad y variabilidad del fenómeno, sujeto a numerosos factores socio-económicos difícilmente previsibles en el tiempo, pero fácilmente monitorizables según unos patrones determinados vinculados a la normatividad, el número de habitantes, el ahorro medio, etc. Como consecuencia, para el diseño del sistema de optimización de la vivienda crecedera, se utilizaron patrones evolutivos. Dichos patrones, alejados ya del concepto espacial y morfológico usualmente utilizado en arquitectura por figuras como C. Alexander o J. Habraken, pasaron a entenderse como una secuencia de eventos en el tiempo (espaciales, sociales, económicos, legales, etc.), que describen el proceso de transformación y que son peculiares de cada vivienda. De esta manera, el tiempo adquirió una especial importancia al convertirse en otro material más del proyecto arquitectónico. Fue en la construcción de los patrones donde se identificaron los mencionados mecanismos de ampliación, entendidos también como sistemas de compactación de la ciudad a través de la ocupación tridimensional del espacio. Al estudiar la densidad, mediante los conceptos de holgura y hacinamiento, se aceptó la congestión de las ciudades como un valor positivo. De esta forma, las posibles transformaciones realizadas por los habitantes (previstas desde un inicio) sobre el escenario del habitar (vivienda semilla), se convirtieron también en herramientas de proyecto urbano que responden a condicionantes del lugar y de los habitantes con distintas intensidades de crecimiento, ocupación y densidad. Igualmente, en el proceso de diseño del sistema de optimización, se detectaron las estrategias para la adaptabilidad y transformación de la casa crecedera, es decir, aquella serie de acciones encaminadas a la alteración de la vivienda para facilitar su ampliación, y que engloban desde sistemas constructivos en espera, que facilitan las costuras entre crecimiento y vivienda semilla, hasta sistemas espaciales que permiten que la casa altere su uso transformándose en un hábitat productivo o en un artefacto de renta. Así como los mecanismos de ampliación están asociados a la morfología, se descubrió que su uso es independiente de la localización, y que las estrategias de adaptabilidad de la vivienda se encuentran ligadas a sistemas constructivos o procesos de gestión vinculados a una región concreta. De esta manera, la combinación de los mecanismos con las estrategias caracterizan el proceso de evolución de la vivienda, vinculándola a unos determinados condicionantes sociales, geográficos y por tanto, constructivos. Finalmente, a través de la adecuada combinación de mecanismos de ampliación y estrategias de adaptabilidad en el proyecto de la vivienda con crecimiento programado es posible optimizar su desarrollo en términos económicos, constructivos, sociales y espaciales. Como resultado, esto ayudaría no sólo a mejorar la vida de los habitantes de la vivienda semilla en términos cualitativos y cuantitativos, sino también a compactar las ciudades mediante sistemas incluyentes, ya que las casas crecederas proporcionan una mayor complejidad de usos y diversidad de relaciones sociales. ABSTRACT The growing house concept -as we currently know it- was used for the first time back in 1932 in the competition Das Wachsende Haus organized by Martin Wagner and Hans Poelzig during the International Exhibition Sonne, Luft und Haus für alle, promoted by Berlin's Tourist Office. In that competition this type of housing was defined as a basic cell or a seed house unit, and depending on the needs and capabilities of the residents it could grow by adding rooms and defining itself as a complete house unit during each growing stage. Many world-top class architects such as Walter Gropius, Bruno Taut, Erich Mendelsohn or Hans Scharoun, were part of this competition exploring a new path in the flexible housing field, the scheduled grownth. From that moment and on, in Europe -and subsequently in the USA and other developed areas- many theorical and pragmatical researchs were directed towards the growing house phenomena, coming from an initial approach related to innovation, spacial and technical innovation. Furthermore -inside the traditional architecture frame in other countries, growing houses were already tested in the XVIII century- mainly due to the size were more affordable in the Real State Market. Since the 30's decade many developing countries had to deal with massive migration movements from the countryside to cities, building large housing developments were -in many cases- formed by growing housing units. In all of these developing countries the growing house approach was drawn from a different perspective than in the developed countries. An economy of means was prioritized, the utilization of low cost construction systems and -in many cases- a guided self-construction was prioritized versus the prefabricated constructions set by specialized technics that were proposed -for instance- in the European cases. To proceed with this research, information from these -and other- housing units was gathered. From then and on different ways to perform the growing actions were identified, according to its relative position from the seed house unit, these ways were named as addition or enlargement mechanisms indifferently utilized without adknowledging the geographic location for each house. The question of why one addition mechanism is preferred over another in any given case became the main target of this Thesis; the ellaboration of an analysis and diagnosis system for the growing house -according to certain parameters- would allow to point out which is the addition or addition process more efficient for a certain family in a particular location. As a starting point the grownth of the housing unit is directly linked to the evolution of the family unit that lives on it, so the house becomes a dynamic habitat. The complexity and the variability of the phenomena was taken into consideration related to a great number of socio-economic factors hardly able to be foreseen ahead on time but easy to be monitored according to certain patterns linked to regulation, population, average savings, etc As a consequence, to design the optimization system for the growing house, evolutionary patterns were utilized. Those patterns far away from the spatial and morphologic concept normally utilized in Architecture by characters like C. Alexander or J. Habraken, started to be understood like a sequence of events on time (spatial events, social events, economic events, legal events, etc) that describes the transformation process and that are particular for each housing unit. Therefore time became something important as another ingredient in the Architectural Project. The before mentioned addition or enlargement mechanisms were identified while building the patterns; these mechanisms were also understood as city's system of compactation through the tridimendional ocupation of space. Studying density, thorough the concepts of comfort and overcrowding, traffic congestion in the city was accepted as a positive value. This way, the possible transformations made by the residents (planned from the begining) about the residencial scenary (seed house), also became tools of the urban project that are a response to site's distinctive features and to the residents with different grownth intensities, activities and density Likewise, during the process of designing the optimization system, strategies for adaptations and transformation of the growing house were detected, in other words, the serial chain of actions directed to modify the house easing its enlargement or addition, and that comprehends from constructive systems on hold -that smooths the costures between grownth and housing seed- to spatial systems that allows that the house modify its utilization, becoming a productive habitat or a rental asset. Because the enlargement mechanisms are linked to the morphology, it was discovered that the use it's not related to the location, and that the adaptation strategies of the houses are linked to constructive systems or management processes linked to a particular area. This way the combination of mechanisms and strategies characterizes the process of housing evolution, linking it to certain social and geographic peculiarities and therefore constructives. At last, through the certain combination of enlargement mechanisms and adaptability strategies in the housing with scheduled grownth project is possible to optimize its development in economic, constructive, social and spatial terms. As a result, this would help not only to improve the life of the seed house residents in qualitative and quantitative terms but also to compact the cities through inclusive systems, given that the growing houses provide a larger complexity of uses and social relations.

Evidence for a structural motif in toxins and interleukin-2 that may be responsible for binding to endothelial cells and initiating vascular leak syndrome

The dose-limiting toxicity of interleukin-2 (IL-2) and immunotoxin (IT) therapy in humans is vascular leak syndrome (VLS). VLS has a complex etiology involving damage to vascular endothelial cells (ECs), extravasation of fluids and proteins, interstitial edema, and organ failure. IL-2 and ITs prepared with the catalytic A chain of the plant toxin, ricin (RTA), and other toxins, damage human ECs in vitro and in vivo. Damage to ECs may initiate VLS; if this damage could be avoided without losing the efficacy of ITs or IL-2, larger doses could be administered. In this paper, we provide evidence that a three amino acid sequence motif, (x)D(y), in toxins and IL-2 damages ECs. Thus, when peptides from RTA or IL-2 containing this sequence motif are coupled to mouse IgG, they bind to and damage ECs both in vitro and, in the case of RTA, in vivo. In contrast, the same peptides with a deleted or mutated sequence do not. Furthermore, the peptide from RTA attached to mouse IgG can block the binding of intact RTA to ECs in vitro and vice versa. In addition, RTA, a fragment of Pseudomonas exotoxin A (PE38-lys), and fibronectin also block the binding of the mouse IgG-RTA peptide to ECs, suggesting that an (x)D(y) motif is exposed on all three molecules. Our results suggest that deletions or mutations in this sequence or the use of nondamaging blocking peptides may increase the therapeutic index of both IL-2, as well as ITs prepared with a variety of plant or bacterial toxins.

From ab initio quantum mechanics to molecular neurobiology: A cation–π binding site in the nicotinic receptor

The nicotinic acetylcholine receptor is the prototype ligand-gated ion channel. A number of aromatic amino acids have been identified as contributing to the agonist binding site, suggesting that cation–π interactions may be involved in binding the quaternary ammonium group of the agonist, acetylcholine. Here we show a compelling correlation between: (i) ab initio quantum mechanical predictions of cation–π binding abilities and (ii) EC50 values for acetylcholine at the receptor for a series of tryptophan derivatives that were incorporated into the receptor by using the in vivo nonsense-suppression method for unnatural amino acid incorporation. Such a correlation is seen at one, and only one, of the aromatic residues—tryptophan-149 of the α subunit. This finding indicates that, on binding, the cationic, quaternary ammonium group of acetylcholine makes van der Waals contact with the indole side chain of α tryptophan-149, providing the most precise structural information to date on this receptor. Consistent with this model, a tethered quaternary ammonium group emanating from position α149 produces a constitutively active receptor.

A streptavidin mutant with altered ligand-binding specificity

The biotin-binding site of streptavidin was modified to alter its ligand-binding specificity. In natural streptavidin, the side chains of N23 and S27 make two of the three hydrogen bonds with the ureido oxygen of biotin. These two residues were mutated to severely weaken biotin binding while attempting to maintain the affinity for two biotin analogs, 2-iminobiotin and diaminobiotin. Redesigning of the biotin-binding site used the difference in local electrostatic charge distribution between biotin and these biotin analogs. Free energy calculations predicted that the introduction of a negative charge at the position of S27 plus the mutation N23A should disrupt two of the three hydrogen bonds between natural streptavidin and the ureido oxygen of biotin. In contrast, the imino hydrogen of 2-iminobiotin should form a hydrogen bond with the side chain of an acidic amino acid at position 27. This should reduce the biotin-binding affinity by approximately eight orders of magnitude, while leaving the affinities for these biotin analogs virtually unaffected. In good agreement with these predictions, a streptavidin mutant with the N23A and S27D substitutions binds 2-iminobiotin with an affinity (Ka) of 1 × 106 M−1, two orders of magnitude higher than that for biotin (1 × 104 M−1). In contrast, the binding affinity of this streptavidin mutant for diaminobiotin (2.7 × 104 M−1) was lower than predicted (2.9 × 105 M−1), suggesting the position of the diaminobiotin in the biotin-binding site was not accurately determined by modeling.

Nuclear Ferritin Protects DNA From UV Damage in Corneal Epithelial Cells

Previously, we identified the heavy chain of ferritin as a developmentally regulated nuclear protein of embryonic chicken corneal epithelial cells. The nuclear ferritin is assembled into a supramolecular form indistinguishable from the cytoplasmic form of ferritin found in other cell types and thus most likely has iron-sequestering capabilities. Free iron, via the Fenton reaction, is known to exacerbate UV-induced and other oxidative damage to cellular components, including DNA. Since corneal epithelial cells are constantly exposed to UV light, we hypothesized that the nuclear ferritin might protect the DNA of these cells from free radical damage. To test this possibility, primary cultures of cells from corneal epithelium and stroma, and from skin epithelium and stroma, were UV irradiated, and DNA strand breaks were detected by an in situ 3′-end labeling method. Corneal epithelial cells without nuclear ferritin were also examined. We observed that the corneal epithelial cells with nuclear ferritin had significantly less DNA breakage than other cell types examined. Furthermore, increasing the iron concentration of the culture medium exacerbated the generation of UV-induced DNA strand breaks in corneal and skin fibroblasts, but not in the corneal epithelial cells. Most convincingly, corneal epithelial cells in which the expression of nuclear ferritin was inhibited became much more susceptible to UV-induced DNA damage. Therefore, it seems that corneal epithelial cells have evolved a novel, nuclear ferritin-based mechanism for protecting their DNA against UV damage.

A specific transition state for S-peptide combining with folded S-protein and then refolding

We measured the folding and unfolding kinetics of mutants for a simple protein folding reaction to characterize the structure of the transition state. Fluorescently labeled S-peptide analogues combine with S-protein to form ribonuclease S analogues: initially, S-peptide is disordered whereas S-protein is folded. The fluorescent probe provides a convenient spectroscopic probe for the reaction. The association rate constant, kon, and the dissociation rate constant, koff, were both determined for two sets of mutants. The dissociation rate constant is measured by adding an excess of unlabeled S-peptide analogue to a labeled complex (RNaseS*). This strategy allows kon and koff to be measured under identical conditions so that microscopic reversibility applies and the transition state is the same for unfolding and refolding. The first set of mutants tests the role of the α-helix in the transition state. Solvent-exposed residues Ala-6 and Gln-11 in the α-helix of native RNaseS were replaced by the helix destabilizing residues glycine or proline. A plot of log kon vs. log Kd for this series of mutants is linear over a very wide range, with a slope of −0.3, indicating that almost all of the molecules fold via a transition state involving the helix. A second set of mutants tests the role of side chains in the transition state. Three side chains were investigated: Phe-8, His-12, and Met-13, which are known to be important for binding S-peptide to S-protein and which also contribute strongly to the stability of RNaseS*. Only the side chain of Phe-8 contributes significantly, however, to the stability of the transition state. The results provide a remarkably clear description of a folding transition state.

Human interleukin-10-related T cell-derived inducible factor: Molecular cloning and functional characterization as an hepatocyte-stimulating factor

IL-10-related T cell-derived inducible factor (IL-TIF or IL-21) is a new cytokine structurally related to IL-10 and originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. Here, we report the cloning of the human IL-TIF cDNA, which shares 79% amino acid identity with mouse IL-TIF and 25% identity with human IL-10. Recombinant human IL-TIF was found to activate signal transducer and activator of transcription factors-1 and -3 in several hepatoma cell lines. IL-TIF stimulation of HepG2 human hepatoma cells up-regulated the production of acute phase reactants such as serum amyloid A, α1-antichymotrypsin, and haptoglobin. Although IL-10 and IL-TIF have distinct activities, antibodies directed against the β chain of the IL-10 receptor blocked the induction of acute phase reactants by IL-TIF, indicating that this chain is a common component of the IL-10 and IL-TIF receptors. Similar acute phase reactant induction was observed in mouse liver upon IL-TIF injection, and IL-TIF expression was found to be rapidly increased after lipopolysaccharide (LPS) injection, suggesting that this cytokine contributes to the inflammatory response in vivo.

Polarity and permeation profiles in lipid membranes

The isotropic 14N-hyperfine coupling constant, a\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{o}^{N}}}\end{equation*}\end{document}, of nitroxide spin labels is dependent on the local environmental polarity. The dependence of a\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{o}^{N}}}\end{equation*}\end{document} in fluid phospholipid bilayer membranes on the C-atom position, n, of the nitroxide in the sn-2 chain of a spin-labeled diacyl glycerophospholipid therefore determines the transmembrane polarity profile. The polarity variation in phospholipid membranes, with and without equimolar cholesterol, is characterized by a sigmoidal, trough-like profile of the form {1 + exp [(n − no)/λ]}−1, where n = no is the point of maximum gradient, or polarity midpoint, beyond which the free energy of permeation decreases linearly with n, on a characteristic length-scale, λ. Integration over this profile yields a corresponding expression for the permeability barrier to polar solutes. For fluid membranes without cholesterol, no ≈ 8 and λ ≈ 0.5–1 CH2 units, and the permeability barrier introduces an additional diffusive resistance that is equivalent to increasing the effective membrane thickness by 35–80%, depending on the lipid. For membranes containing equimolar cholesterol, no ≈ 9–10, and the total change in polarity is greater than for membranes without cholesterol, increasing the permeability barrier by a factor of 2, whereas the decay length remains similar. The permeation of oxygen into fluid lipid membranes (determined by spin-label relaxation enhancements) displays a profile similar to that of the transmembrane polarity but of opposite sense. For fluid membranes without cholesterol no ≈ 8 and λ ≈ 1 CH2 units, also for oxygen. The permeation profile for polar paramagnetic ion complexes is closer to a single exponential decay, i.e., no lies outside the acyl-chain region of the membrane. These results are relevant not only to the permeation of water and polar solutes into membranes and their permeabilities, but also to depth determinations of site-specifically spin-labeled protein residues by using paramagnetic relaxation agents.

Photochemical energy conversion in a helical oligoproline assembly.

A general method is described for constructing a helical oligoproline assembly having a spatially ordered array of functional sites protruding from a proline-II helix. Three different redox-active carboxylic acids were coupled to the side chain of cis-4-amino-L-proline. These redox modules were incorporated through solid-phase peptide synthesis into a 13-residue helical oligoproline assembly bearing in linear array a phenothiazine electron donor, a tris(bipyridine)ruthenium(II) chromophore, and an anthraquinone electron acceptor. Upon transient 460-nm irradiation in acetonitrile, this peptide triad formed with 53% efficiency an excited state containing a phenothiazine radical cation and an anthraquinone radical anion. This light-induced redox-separated state had a lifetime of 175 ns and stored 1.65 eV of energy.

Unexpected beta2-microglobulin sequence diversity in individual rainbow trout.

For mammals beta2-microglobulin (beta2m), the light chain of major histocompatibility complex (MHC) class I molecules, is invariant (or highly conserved) and is encoded by a single gene unlinked to the MHC. We find that beta2m of a salmonid fish, the rainbow trout (Oncorhynchus mykiss), does not conform to the mammalian paradigm. Ten of 12 randomly selected beta2m cDNA clones from an individual fish have different nucleotide sequences. A complex restriction fragment length polymorphism pattern is observed with rainbow trout, suggesting multiple beta2m genes in the genome, in excess of the two genes expected from the ancestral salmonid tetraploidy. Additional duplication and diversification of the beta2m genes might have occurred subsequently. Variation in the beta2m cDNA sequences is mainly at sites that do not perturb the structure of the mature beta2m protein, showing that the observed diversity of the trout beta2m genes is not primarily a result of pathogen selection.

Object-related activity revealed by functional magnetic resonance imaging in human occipital cortex.

The stages of integration leading from local feature analysis to object recognition were explored in human visual cortex by using the technique of functional magnetic resonance imaging. Here we report evidence for object-related activation. Such activation was located at the lateral-posterior aspect of the occipital lobe, just abutting the posterior aspect of the motion-sensitive area MT/V5, in a region termed the lateral occipital complex (LO). LO showed preferential activation to images of objects, compared to a wide range of texture patterns. This activation was not caused by a global difference in the Fourier spatial frequency content of objects versus texture images, since object images produced enhanced LO activation compared to textures matched in power spectra but randomized in phase. The preferential activation to objects also could not be explained by different patterns of eye movements: similar levels of activation were observed when subjects fixated on the objects and when they scanned the objects with their eyes. Additional manipulations such as spatial frequency filtering and a 4-fold change in visual size did not affect LO activation. These results suggest that the enhanced responses to objects were not a manifestation of low-level visual processing. A striking demonstration that activity in LO is uniquely correlated to object detectability was produced by the "Lincoln" illusion, in which blurring of objects digitized into large blocks paradoxically increases their recognizability. Such blurring led to significant enhancement of LO activation. Despite the preferential activation to objects, LO did not seem to be involved in the final, "semantic," stages of the recognition process. Thus, objects varying widely in their recognizability (e.g., famous faces, common objects, and unfamiliar three-dimensional abstract sculptures) activated it to a similar degree. These results are thus evidence for an intermediate link in the chain of processing stages leading to object recognition in human visual cortex.

Influência do LPS e do zinco na interação mãe e filhote

O comportamento materno consiste em um conjunto de mudanças comportamentais e fisiológicas, exercidas pelos indivíduos adultos em torno dos indivíduos reprodutivamente imaturos, garantindo sua sobrevivência e a propagação de sua espécie. A interação mãe e filhote é tida tipicamente como simbiótica. Os filhotes quando separados da mãe sinalizam para serem recolhidos através de dicas olfativas, visuais e da vocalização que representa uma forma de comunicação filhote e mãe. O modelo de febre clássico e amplamente empregado envolve a utilização do lipopolissacarídeo (LPS), principal componente da parede celular de bactérias Gram-Negativas. Além da febre, as infecções apresentam uma cadeia de respostas não especificas do hospedeiro que se sabe estarem envolvidos em muitas das funções vitais, incluindo a resposta imune estas incluem a hipozinquemia. Sendo assim, fêmeas virgens, gestantes e lactantes receberam LPS (100 µg/kg, i.p.) e foram tratadas com zinco (2 mg/kg, s.c.) O peso corporal, consumo de água, ração, e a temperatura corporal foram medidas por noventa e seis horas, duas horas após a administração do LPS. No quinto dia de lactação foram observados o comportamento maternal, a atividade geral em campo aberto e a vocalização ultrassônica nos filhotes. No dia do desmame os filhotes dessas fêmeas receberam um desafio com LPS (50 µg/kg, i.p.) e duas horas após a administração, foram observados a atividade geral em campo aberto, e o burst e fagocitose de neutrófilos. Observamos que: 1) Em ratas virgens, gestantes e lactantes, a exposição ao LPS e o tratamento com zinco modificou de forma específica a temperatura e peso corporal, consumo de água e ração e a atividade geral observadas em campo aberto; 2) No período de lactação, houve redução da latência para busca do primeiro filhote. Na prole das fêmeas lactantes verificou-se que: 3) Houve alteração no padrão de vocalização dos filhotes; 4) houve alteração na atividade geral observada em campo aberto e no burst e fagocitose de neutrófilos no vigésimo primeiro dia pós natal, após um desafio com a endotoxina, Assim, os resultados indicam que a administração de LPS e o tratamento com zinco têm seus efeitos modulados conforme o estágio fisiológico em que a fêmea se encontra, e interfere com a interação mãe/filhote, resultando em efeitos de curto e longo prazo sobre o comportamento dos filhotes. A partir deste trabalho, a possibilidade da exposição de mães à endotoxina bacteriana e da modulação de seus efeitos pelo zinco programar as respostas inflamatórias dos filhos torna-se factível

Estudo da microestrutura e dinâmica molecular do Poly(3-(2\'-ethylhexyl)thiophene)(P3EHT) via ressonância magnética nuclear

O estudo da microestrutura e dinâmica molecular de polímeros conjugados é de grande importância para o entendimento das propriedades físicas desta classe de materiais. No presente trabalho utilizou-se técnicas de ressonância magnética nuclear em baixo e alto campo para elucidar os processos de dinâmica molecular e cristalização do polímero Poly(3-(2’-ethylhexyl)thiophene) - P3EHT. O P3EHT é um polímero modelo para tal estudo, pois apresenta temperatura de fusão bem inferior a sua temperatura de degradação. Esta característica permite acompanhar os processos de cristalização in situ utilizando RMN. Além disso, sua similaridade ao já popular P3HT o torna um importante candidato a camada ativa em dispositivos eletrônicos orgânicos. O completo assinalamento do espectro de 13C para o P3EHT foi realizado utilizando as técnicas de defasamento dipolar e HETCOR. Os processos de dinâmica molecular, por sua vez, foram sondados utilizando DIPSHIFT. Observou-se um gradiente de mobilidade na cadeia lateral do polímero. Além disso, os baixos valores de parametros de ordem obtidos em comparação a experimentos similares realizados no P3HT na literatura indicam um aparente aumento no volume livre entre cadeias consecutivas na fase cristalina. Isso indica que a presença do grupo etil adicional no P3EHT causa um completo rearranjo das moléculas e dificulta seu empacotamento. Constatou-se ainda pouca variação das curvas de DIPSHIFT para os carbonos da cadeia lateral como função do método de excitação utilizado, o que aponta para um polímero que apresenta cadeia lateral móvel mesmo em sua fase cristalina. Os dados de dinâmica molecular foram corroborados por medidas de T1, T1ρ e TCH. Utilizando filtros dipolares em baixo campo observou-se três temperaturas de transição para o P3EHT: 250 K, 325 K e 350 K. A cristalização desse material é um processo lento. Verificou-se que o mesmo pode se estender por até até 24h a temperatura ambiente. Mudanças no espectro de 13C utilizando CPMAS em alto campo indicam um ordenamento dos anéis tiofeno (empacotamento π – π) como o principal processo de cristalização para o P3EHT.

Competitividade da cadeia produtiva do Arapaima gigas, o pirarucu da Amazônia brasileira

Com um quinto da água doce do planeta, o sistema fluvial da Amazônia apresenta um enorme potencial para piscicultura. De acordo com a FAO, em função das suas condições geográficas, o Brasil é um dos poucos países que tem condições de atender à crescente demanda mundial, podendo tornar-se um dos maiores produtores de peixes do mundo. A observação desse potencial amazônico motivou o desenvolvimento desta pesquisa que se debruça sobre a questão da competitividade da cadeia produtiva de Arapaima gigas, o pirarucu da Amazônia brasileira. As pesquisas de campo levaram ao mapeamento de duas cadeias: a cadeia extrativista e a piscicultura. A abordagem sistêmica permitiu a verificação das características dos atores e as bases sob as quais as transações se estabelecem. À luz da teoria das restrições foram identificados os gargalos que impedem a competitividade do sistema, inclusive alertando para os recursos com restrição de capacidade. Comprovou-se que a falta de uma cadeia produtiva devidamente organizada pode provocar graves prejuízos a determinados elos, enquanto outros membros aproveitam-se de ações oportunistas para ampliar suas margens de lucro. Da mesma forma, a ausência de uma cadeia produtiva completa impede a fixação do valor gerado na região de origem das matérias-primas. No entanto, também foi possível comprovar que há possibilidade de desenvolver o extrativismo atribuindo valor econômico aos recursos naturais e gerando renda para a comunidade local. Além de apresentar o panorama do setor na região delimitada, este estudo culminou em reflexões capazes de orientar políticas públicas para o desenvolvimento de cadeias produtivas completas na região da Amazônia brasileira.

Modelo conceitual para comissionamento de sistemas prediais.

Atualmente, os edifícios, em função dos avanços tecnológicos dos sistemas prediais, necessitam de maior planejamento, detalhamento de projetos, controles de execução e treinamento dos profissionais de operação e manutenção para atender os requisitos de projeto do proprietário, que têm como premissa os conceitos de sustentabilidade, qualidade e desempenho. A presença dos sofisticados sistemas de controle contribui para facilitar o gerenciamento de insumos como água e energia, porém pequenas falhas podem levar a grandes falhas de desempenho. As falhas na concepção dos edifícios têm início com a má interpretação, por parte da equipe técnica, dos requisitos dos proprietários. Assim, é necessário que na concepção do edifício sejam verificados todos os requisitos solicitados pelos proprietários para o edifício durante o seu ciclo de vida. A falha da comunicação percorre toda a cadeia produtiva do edifício gerando falhas de planejamento, projeto, execução e manutenção. O comissionamento é um processo para atender aos requisitos de projeto do proprietário, documentar as fases do ciclo de vida dos edifícios, capacitar os profissionais de operação e manutenção, com o objetivo de evitar as falhas, diminuir desperdícios e retrabalhos, melhorar a qualidade, o desempenho e a sustentabilidade dos edifícios. O comissionamento é mais difundido em sistemas prediais de ar condicionado e de iluminação tendo como meta a alta eficiência energética e a economia de água, sendo pouco utilizado no Brasil. Neste contexto, o objetivo desta pesquisa é propor um modelo conceitual de comissionamento para sistemas prediais. A metodologia adotada para o desenvolvimento do modelo, utiliza a pesquisa bibliográfica como procedimento técnico. Para elucidar em que fase o comissionamento é aplicado, este foi relacionado com os outros conceitos utilizados no ciclo de vida do edifício como a coordenação de projeto, o gerenciamento de execução, o gerenciamento de facilidades, a qualidade, o desempenho, a sustentabilidade. Para o desenvolvimento do modelo conceitual é apresentado um fluxo das fases do ciclo de vida e respectivas etapas do comissionamento e outro fluxo com a relação de documentos gerados em cada fase. O resultado, ou seja, o modelo conceitual dá as diretrizes para o desenvolvimento de um comissionamento por meio da descrição das atividades, das competências e dos produtos gerados em cada etapa do comissionamento, conforme as fases do ciclo do edifício. Este trabalho contribui para difundir o comissionamento e embasar a sua aplicação em edifícios.