Gordon Matta-Clark a través de Rem Koolhaas : adición a través de eliminación

Gordon Matta-Clark es el origen de la presente investigación. Su obra, de total actualidad, ha ido ganando interés entre la comunidad de arquitectos, si bien su dimensión teórica se ha visto a menudo eclipsada por el poderoso atractivo de sus aberturas, cortes y extracciones. Son muchos los investigadores que detectan una carencia en el estudio de su corpus de pensamiento desde el campo de la crítica arquitectónica a la que está inevitablemente vinculado, a pesar de haber puesto en marcha una potente maquinaria de criticismo. Se constata a su vez la problemática existente al construir dialécticas entre la obra de Gordon Matta-Clark y el ámbito de la práctica arquitectónica. Surgen áreas forzadas de reflexión al elevar ambas esferas al mismo plano, achacado a un desconocimiento de las motivaciones críticas e históricas del proyecto del arquitecto. Aparece de modo recurrente un intento por establecer una dialéctica que, sin embargo, es relegada constantemente a una colección de notas al pie de página: Rem Koolhaas es sesgadamente mencionado por la crítica como el digno continuador accidental de las teorías de Gordon Matta-Clark. Se inicia así la construcción de una dialéctica que permanece aún sin resolver. Rem Koolhaas, en una entrevista en la que vierte su opinión sobre Gordon Matta-Clark, lo considera el precursor accidental de su ‘estrategia de vacío’, reconociendo en su trabajo procesos de adición a través de eliminación como método de creación de espacio arquitectónico. Una visión incisiva que desdeña su faceta de artista destructor destacando su habilidad para inocular el vacío mediante el empleo de herramientas de adición a través de eliminación. Estas premisas dan lugar de forma automática a la hipótesis de partida: la formalización de una investigación que llene ese vacío estableciendo un fuego cruzado entre ambos arquitectos, los cuales comparten de manera fortuita época y escenarios. El discurso de Rem Koolhaas iluminará el pensamiento de Gordon Matta-Clark vinculando sus respectivas plataformas de producción teórica, construyendo un espacio metafórico común en torno a la palabra y la imagen, herramientas que ambos manejan con habilidad y fluidez. Las propuestas del grupo Anarchitecture y las Art Cards o recortes anárquicos de soliloquios mattaclarkianos encuentran su réplica en el diccionario de términos e imágenes “Small, Medium, Large, Extra-Large”, descrito por Rem Koolhaas como una acumulación de palabras e imágenes que ilumina la condición de la arquitectura actual. La investigación establece una profusa asociación de ideas, imágenes y parlamentos que navegan en el universo referencial de Rem Koolhaas aportando una nueva visión sobre la dimensión crítica de Gordon Matta-Clark. Una visión poliédrica alrededor de cinco conceptos clave y un glosario de estrategias de proyecto que abren nuevos umbrales en los que la adición a través de la eliminación se repite hasta la saciedad transformado en mantra arquitectónico. ABSTRACT The present research finds its origins in Gordon Matta-Clark. His work, still relevant today, has been gaining interest within the community of architects, although its theoretical dimension has often been overshadowed by the powerful attraction of his openings, cuts and extractions. Many researchers detect a lack in the study of his body of thought from the field of architectural criticism to which he is inevitably associated, despite having launched a powerful machinery of criticism. It is noted at the same time the problem appeared when different dialectics between the work of Gordon Matta-Clark and the field of architectural practice are established. Forced areas of thought emerge when both fields are observed from the same point of view, derived from a lack of knowledge of the critical and historical motivations of the architect project. An attempt to establish a dialectical relationship with another architect appears recurrently in different books and writings. However it is steadily consigned to a collection of insignificant footnotes: the critics mention Rem Koolhaas as the worthy accidental successor of the theories of Gordon Matta-Clark. The construction of a dialectic that remains unresolved begins at this point. Gordon Matta-Clark is considered by Rem Koolhaas the accidental predecessor of his ‘strategy of emptiness’ -in an interview in which he pours his opinion on him- recognizing processes of addition through elimination in the work of Matta-Clark as a method of building architectural space. Rem Koolhaas rejects his role as destructor artist highlighting his ability to inoculate the emptiness by using addition tools through elimination. These premises lead us automatically to the hypothesis of the research: the formalization of research that will fill the emptiness by setting a crossfire between both architects, who share by chance the same scenarios and time. The discourse given by Rem Koolhaas illuminates Gordon Matta-Clark´s thought by linking their respective theoretical work platforms, building a common metaphorical space around word and image as tools managed by both of them with expertise and fluency. The Anarchitecture proposals and the Art Cards -anarchic cuts of Matta-Clark´s thought- find its counterpart in the dictionary of terms and images included in “Small, Medium, Large, Extra- Large”, described by Rem Koolhaas as an accumulation of words and images that illuminates the condition of architecture today. The research provides a wide range of associations of ideas, images and discussions around the universe of references of Rem Koolhaas, offering a new insight into the critical dimension of the work of Gordon Matta-Clark. A multifaceted vision around five key concepts and a glossary of project strategies that opens up new thresholds in which addition through elimination is repeated endlessly becoming an architectural mantra.

Development of a sensorimotor algorithm able to deal with unforeseen pushes and its implementation based on VHDL

Development of a Sensorimotor Algorithm Able to Deal with Unforeseen Pushes and Its Implementation Based on VHDL is the title of my thesis which concludes my Bachelor Degree in the Escuela Técnica Superior de Ingeniería y Sistemas de Telecomunicación of the Universidad Politécnica de Madrid. It encloses the overall work I did in the Neurorobotics Research Laboratory from the Beuth Hochschule für Technik Berlin during my ERASMUS year in 2015. This thesis is focused on the field of robotics, specifically an electronic circuit called Cognitive Sensorimotor Loop (CSL) and its control algorithm based on VHDL hardware description language. The reason that makes the CSL special resides in its ability to operate a motor both as a sensor and an actuator. This way, it is possible to achieve a balanced position in any of the robot joints (e.g. the robot manages to stand) without needing any conventional sensor. In other words, the back electromotive force (EMF) induced by the motor coils is measured and the control algorithm responds depending on its magnitude. The CSL circuit contains mainly an analog-to-digital converter (ADC) and a driver. The ADC consists on a delta-sigma modulation which generates a series of bits with a certain percentage of 1's and 0's, proportional to the back EMF. The control algorithm, running in a FPGA, processes the bit frame and outputs a signal for the driver. This driver, which has an H bridge topology, gives the motor the ability to rotate in both directions while it's supplied with the power needed. The objective of this thesis is to document the experiments and overall work done on push ignoring contractive sensorimotor algorithms, meaning sensorimotor algorithms that ignore large magnitude forces (compared to gravity) applied in a short time interval on a pendulum system. This main objective is divided in two sub-objectives: (1) developing a system based on parameterized thresholds and (2) developing a system based on a push bypassing filter. System (1) contains a module that outputs a signal which blocks the main Sensorimotor algorithm when a push is detected. This module has several different parameters as inputs e.g. the back EMF increment to consider a force as a push or the time interval between samples. System (2) consists on a low-pass Infinite Impulse Response digital filter. It cuts any frequency considered faster than a certain push oscillation. This filter required an intensive study on how to implement some functions and data types (fixed or floating point data) not supported by standard VHDL packages. Once this was achieved, the next challenge was to simplify the solution as much as possible, without using non-official user made packages. Both systems behaved with a series of interesting advantages and disadvantages for the elaboration of the document. Stability, reaction time, simplicity or computational load are one of the many factors to be studied in the designed systems. RESUMEN. Development of a Sensorimotor Algorithm Able to Deal with Unforeseen Pushes and Its Implementation Based on VHDL es un Proyecto de Fin de Grado (PFG) que concluye mis estudios en la Escuela Técnica Superior de Ingeniería y Sistemas de Telecomunicación de la Universidad Politécnica de Madrid. En él se documenta el trabajo de investigación que realicé en el Neurorobotics Research Laboratory de la Beuth Hochschule für Technik Berlin durante el año 2015 mediante el programa de intercambio ERASMUS. Este PFG se centra en el campo de la robótica y en concreto en un circuito electrónico llamado Cognitive Sensorimotor Loop (CSL) y su algoritmo de control basado en lenguaje de modelado hardware VHDL. La particularidad del CSL reside en que se consigue que un motor haga las veces tanto de sensor como de actuador. De esta manera es posible que las articulaciones de un robot alcancen una posición de equilibrio (p.ej. el robot se coloca erguido) sin la necesidad de sensores en el sentido estricto de la palabra. Es decir, se mide la propia fuerza electromotriz (FEM) inducida sobre el motor y el algoritmo responde de acuerdo a su magnitud. El circuito CSL se compone de un convertidor analógico-digital (ADC) y un driver. El ADC consiste en un modulador sigma-delta, que genera una serie de bits con un porcentaje de 1's y 0's determinado, en proporción a la magnitud de la FEM inducida. El algoritmo de control, que se ejecuta en una FPGA, procesa esta cadena de bits y genera una señal para el driver. El driver, que posee una topología en puente H, provee al motor de la potencia necesaria y le otorga la capacidad de rotar en cualquiera de las dos direcciones. El objetivo de este PFG es documentar los experimentos y en general el trabajo realizado en algoritmos Sensorimotor que puedan ignorar fuerzas de gran magnitud (en comparación con la gravedad) y aplicadas en una corta ventana de tiempo. En otras palabras, ignorar empujones conservando el comportamiento original frente a la gravedad. Para ello se han desarrollado dos sistemas: uno basado en umbrales parametrizados (1) y otro basado en un filtro de corte ajustable (2). El sistema (1) contiene un módulo que, en el caso de detectar un empujón, genera una señal que bloquea el algoritmo Sensorimotor. Este módulo recibe diferentes parámetros como el incremento necesario de la FEM para que se considere un empujón o la ventana de tiempo para que se considere la existencia de un empujón. El sistema (2) consiste en un filtro digital paso-bajo de respuesta infinita que corta cualquier variación que considere un empujón. Para crear este filtro se requirió un estudio sobre como implementar ciertas funciones y tipos de datos (coma fija o flotante) no soportados por las librerías básicas de VHDL. Tras esto, el objetivo fue simplificar al máximo la solución del problema, sin utilizar paquetes de librerías añadidos. En ambos sistemas aparecen una serie de ventajas e inconvenientes de interés para el documento. La estabilidad, el tiempo de reacción, la simplicidad o la carga computacional son algunas de las muchos factores a estudiar en los sistemas diseñados. Para concluir, también han sido documentadas algunas incorporaciones a los sistemas: una interfaz visual en VGA, un módulo que compensa el offset del ADC o la implementación de una batería de faders MIDI entre otras.

The Double‐Edged Sword Of Interdict: Some More Warnings From History

In this paper I revisit the age-old question of the relationship between philosophy and theology by rejecting the claim that throughout the history of the Christian Church, whenever Christian thinkers have baptized philosophy, they have done so to the detriment of theology. Church history reveals just the opposite, i. e., that sometimes theologians have creatively and fruitfully used philosophical language, concepts, methods, and conclusions to understand and express the faith. In addition, church history records numerous attempts to limit philosophical enquiry for theological reasons that proved unsuccessful and counter-productive. Both types of interaction between philosophy and theology occurred at the University of Paris during the thirteenth century. Despite repeated efforts of some officials to place philosophy under interdict, that is, to ban the reading of particular philosophical works or the teaching of philosophical propositions from the university faculties, a series of university theologians applied Aristotelian tools of enquiry to questions about the Christian faith with positive and constructive results. If academic theology at Paris during the thirteenth century has anything to teach us, it is that interdict cuts both ways. It might protect some theological claims from philosophical contamination or compromise, but it can also insulate theological claims from much needed critical analysis. The thinkers and developments surveyed in this paper suggest that perhaps instead of placing deconstruction under interdict, today’s Christian thinkers should use some of the language, concepts, methods and conclusions of Derrida to further theological understanding.

Translocation and specific cleavage of bacteriophage T7 DNA in vivo by EcoKI

Infection of Escherichia coli containing the type I restriction enzyme EcoKI by bacteriophage T7 0.3 mutants leads to restriction during the late stages of genome entry and during DNA replication. Patterns of cleavage in vivo suggest that some cutting occurs near the midpoint of two recognition sites, consistent with the idea that EcoKI translocates DNA bidirectionally through itself and cuts when two enzyme molecules collide. Rapid ejection of a 0.3+ T7 genome from a bacteriophage λ particle results in degradation of the infecting DNA by EcoKI, showing that the normal T7 DNA translocation process delays restriction. A unique recognition site inserted at the genomic left end allows EcoKI to function as a molecular motor and to translocate the remaining 39 kilobases of T7 DNA into the cell.

Functional topography of nascent RNA in elongation intermediates of RNA polymerase

To determine the dynamics of transcript extrusion from Escherichia coli RNA polymerase (RNAP), we used degradation of the RNA by RNases T1 and A in a series of consecutive elongation complexes (ECs). In intact ECs, even extremely high doses of the RNases were unable to cut the RNA closer than 14–16 nt from the 3′ end. Our results prove that all of the cuts detected within the 14-nt zone are derived from the EC that is denatured during inactivation of the RNases. The protected zone monotonously translocates along the RNA after addition of new nucleotides to the transcript. The upstream region of the RNA heading toward the 5′ end is cleaved and dissociated from the EC, with no effect on the stability and activity of the EC. Most of the current data suggest that an 8- to 10-nt RNA⋅DNA hybrid is formed in the EC. Here, we show that an 8- to 10-nt RNA obtained by truncating the RNase-generated products further with either GreB or pyrophosphate is sufficient for the high stability and activity of the EC. This result suggests that the transcript–RNAP interaction that is required for holding the EC together can be limited to the RNA region involved in the 8- to 10-nt RNA⋅DNA hybrid.

Activity of individual ERCC1 and XPF subunits in DNA nucleotide excision repair

ERCC1–XPF is a structure-specific nuclease with two subunits, ERCC1 and XPF. The enzyme cuts DNA at junctions where a single strand moves 5′ to 3′ away from a branch point with duplex DNA. This activity has a central role in nucleotide excision repair (NER), DNA cross-link repair and recombination. To dissect the activities of the nuclease it is necessary to investigate the subunits individually, as studies of the enzyme so far have only used the heterodimeric complex. We produced recombinant ERCC1 and XPF separately in Escherichia coli as soluble proteins. Activity was monitored by a sensitive dual incision assay for NER by complementation of cell extracts. XPF and ERCC1 are unstable in mammalian cells in the absence of their partners but we found, surprisingly, that ERCC1 alone could confer some repair to extracts from ERCC1-defective cells. A version of ERCC1 lacking the first 88 non-conserved amino acids was also functional. This indicated that a small amount of active XPF was present in ERCC1 extracts, and immunoassays showed this to be the case. Some repair in XPF-defective extracts could be achieved by adding ERCC1 and XPF proteins together, but not by adding only XPF. The results show for the first time that functional ERCC1–XPF can be formed from separately produced subunits. Protein sequence comparison revealed similarity between the ERCC1 family and the C-terminal region of the XPF family, including the regions of both proteins that are necessary for the ERCC1–XPF heterodimeric interaction. This suggests that the ERCC1 and XPF families are related via an ancient duplication.

Granzymes A and B directly cleave lamins and disrupt the nuclear lamina during granule-mediated cytolysis

Cytotoxic T lymphocytes (CTL) induce apoptosis by engaging death receptors or by exocytosis of cytolytic granules containing granzyme (Gzm) proteases and perforin. The lamins, which maintain the structural integrity of the nuclear envelope, are cleaved by caspases during caspase-mediated apoptosis. Although death receptor engagement and GzmB activate caspases, CTL also induce apoptosis during caspase blockade. Both GzmA and GzmB directly and efficiently cleave laminB in vitro, in situ in isolated nuclei and in cells loaded with perforin and Gzms, even in the presence of caspase inhibitors. LaminB is cleaved by GzmA at concentrations of 3 nM, but GzmB is 50 times less active. GzmA cuts laminB at R392; GzmB cuts at the caspase VEVD231 site. Characteristic laminB fragments generated by Gzm proteolysis also are observed during CTL lysis, even in the presence of caspase inhibitors or in cells overexpressing bcl-2. Lamins A/C are direct substrates of GzmA, but not GzmB. GzmA and GzmB therefore directly target critical caspase substrates in caspase-resistant cells.

Distinct neurochemical features of acute and persistent pain

To address the neurochemistry of the mechanisms that underlie the development of acute and persistent pain, our laboratory has been studying mice with deletions of gene products that have been implicated in nociceptive processing. We have recently raised mice with a deletion of the preprotachykinin-A gene, which encodes the peptides substance P (SP) and neurokinin A (NKA). These studies have identified a specific behavioral phenotype in which the animals do not detect a window of “pain” intensities; this window cuts across thermal, mechanical, and chemical modalities. The lowered thermal and mechanical withdrawal thresholds that are produced by tissue or nerve injury, however, were still present in the mutant mice. Thus, the behavioral manifestations of threshold changes in nociceptive processing in the setting of injury do not appear to require SP or NKA. To identify relevant neurochemical factors downstream of the primary afferent, we are also studying the dorsal horn second messenger systems that underlie the development of tissue and nerve injury-induced persistent pain states. We have recently implicated the γ isoform of protein kinase C (PKCγ) in the development of nerve injury-induced neuropathic pain. Acute pain processing, by contrast, is intact in the PKCγ-null mice. Taken together, these studies emphasize that there is a distinct neurochemistry of acute and persistent pain. Persistent pain should be considered a disease state of the nervous system, not merely a prolonged acute pain symptom of some other disease conditions.

La roca magica: Uses of natural zeolites in agriculture and industry

For nearly 200 years since their discovery in 1756, geologists considered the zeolite minerals to occur as fairly large crystals in the vugs and cavities of basalts and other traprock formations. Here, they were prized by mineral collectors, but their small abundance and polymineralic nature defied commercial exploitation. As the synthetic zeolite (molecular sieve) business began to take hold in the late 1950s, huge beds of zeolite-rich sediments, formed by the alteration of volcanic ash (glass) in lake and marine waters, were discovered in the western United States and elsewhere in the world. These beds were found to contain as much as 95% of a single zeolite; they were generally flat-lying and easily mined by surface methods. The properties of these low-cost natural materials mimicked those of many of their synthetic counterparts, and considerable effort has made since that time to develop applications for them based on their unique adsorption, cation-exchange, dehydration–rehydration, and catalytic properties. Natural zeolites (i.e., those found in volcanogenic sedimentary rocks) have been and are being used as building stone, as lightweight aggregate and pozzolans in cements and concretes, as filler in paper, in the take-up of Cs and Sr from nuclear waste and fallout, as soil amendments in agronomy and horticulture, in the removal of ammonia from municipal, industrial, and agricultural waste and drinking waters, as energy exchangers in solar refrigerators, as dietary supplements in animal diets, as consumer deodorizers, in pet litters, in taking up ammonia from animal manures, and as ammonia filters in kidney-dialysis units. From their use in construction during Roman times, to their role as hydroponic (zeoponic) substrate for growing plants on space missions, to their recent success in the healing of cuts and wounds, natural zeolites are now considered to be full-fledged mineral commodities, the use of which promise to expand even more in the future.

Raising the bar: the importance of hospital library standards in the continuing medical education accreditation process*

The Connecticut State Medical Society (CSMS) reviews and accredits the continuing medical education (CME) programs offered by Connecticut's hospitals. As part of the survey process, the CSMS assesses the quality of the hospitals' libraries. In 1987, the CSMS adopted the Medical Library Association's (MLA's) “Minimum Standards for Health Sciences Libraries in Hospitals.” In 1990, professional librarians were added to the survey team and, later, to the CSMS CME Committee. Librarians participating in this effort are recruited from the membership of the Connecticut Association of Health Sciences Librarians (CAHSL). The positive results of having a qualified librarian on the survey team and the invaluable impact of adherence to the MLA standards are outlined. As a direct result of this process, hospitals throughout the state have added staffing, increased space, and added funding for resources during an era of cutbacks. Some hospital libraries have been able to maintain a healthy status quo, while others have had proposed cuts reconsidered by administrators for fear of losing valuable CME accreditation status. Creating a relationship with an accrediting agency is one method by which hospital librarians elsewhere may strengthen their efforts to ensure adequate library resources in an era of downsizing. In addition, this collaboration has provided a new and important role for librarians to play on an accreditation team.

Distinct mechanisms underlie activation of hypothalamic neurosecretory neurons and their medullary catecholaminergic afferents in categorically different stress paradigms.

Intermittent electrical footshock induces c-fos expression in parvocellular neurosecretory neurons expressing corticotropin-releasing factor and in other visceromotor cell types of the paraventricular hypothalamic nucleus (PVH). Since catecholaminergic neurons of the nucleus of the solitary tract and ventrolateral medulla make up the dominant loci of footshock-responsive cells that project to the PVH, these were evaluated as candidate afferent mediators of hypothalamic neuroendocrine responses. Rats bearing discrete unilateral transections of this projection system were exposed to a single 30-min footshock session and sacrificed 2 hr later. Despite depletion of the aminergic innervation on the ipsilateral side, shock-induced up-regulation of Fos protein and corticotropin-releasing factor mRNA were comparable in strength and distribution in the PVH on both sides of the brain. This lesion did, however, result in a substantial reduction of Fos expression in medullary aminergic neurons on the ipsilateral side. These results contrast diametrically with those obtained in a systemic cytokine (interleukin 1) challenge paradigm, where similar cuts ablated the Fos response in the ipsilateral PVH but left intact the induction seen in the ipsilateral medulla. We conclude that (i) footshock-induced activation of medullary aminergic neurons is a secondary consequence of stress, mediated via a descending projection transected by our ablation, (ii) stress-induced activation of medullary aminergic neurons is not necessarily predictive of an involvement of these cell groups in driving hypothalamic visceromotor responses to a given stressor, and (iii) despite striking similarities in the complement of hypothalamic effector neurons and their afferents that may be activated by stresses of different types, distinct mechanisms may underlie adaptive hypothalamic responses in each.

Gene disruption of a G4-DNA-dependent nuclease in yeast leads to cellular senescence and telomere shortening.

The yeast gene KEM1 (also named SEP1/DST2/XRN1/RAR5) produces a G4-DNA-dependent nuclease that binds to G4 tetraplex DNA structure and cuts in a single-stranded region 5' to the G4 structure. G4-DNA generated from yeast telomeric oligonucleotides competitively inhibits the cleavage reaction, suggesting that this enzyme may interact with yeast telomeres in vivo. Homozygous deletions of the KEM1 gene in yeast block meiosis at the pachytene stage, which is consistent with the hypothesis that G4 tetraplex DNA may be involved in homologous chromosome pairing during meiosis. We conjectured that the mitotic defects of kem1/sep1 mutant cells, such as a higher chromosome loss rate, are also due to failure in processing G4-DNA, especially at telomeres. Here we report two phenotypes associated with a kem1-null allele, cellular senescence and telomere shortening, that provide genetic evidence that G4 tetraplex DNA may play a role in telomere functioning. In addition, our results reveal that chromosome ends in the same cells behave differently in a fashion dependent on the KEM1 gene product.

Caracterização genotípica de Arcobacter spp. isolados de carnes de aves comercializadas no Município de São Paulo - SP

Arcobacter spp. é um micro-organismo Gram negativo que provoca diarreia aquosa e sepse em seres humanos. A. butzleri, A. cryaerophilus e A. skirrowii são espécies patogênicas para humanos. O objetivo deste estudo foi detectar a presença de Arcobacter spp. na carne de aves comercializadas em açougues na cidade de São Paulo, verificando os genes de virulência e o perfil genotípico. Um total de 300 cortes de carne de frango foram submetidos ao cultivo e isolamento sob condições aeróbicas, a 30°C por 72 horas. Colônias suspeitas de Arcobacter spp. foram selecionadas para a detecção molecular pela reacção em cadeia da polimerase (PCR), a fim de determinar as espécies e os genes de virulência. Os resultados revelaram a presença de Arcobacter spp. em 18.3% (55/300) de amostras de carne de aves, sendo identificado como A. butzleri 63,6% (35/55) e A. cryaerophilus 36,3% (20/55). Os genes de virulência pesquisados demonstraram positividade de 100% (55/55) para o ciaB e mviN, seguidos de cj1349 98,1% (54/55), pldA 94,4% (52/55), cadF 72,7% (40/55), tlyA 92,7% (51/55), hecA 49% (27/55), irgA 47,2% (26/55) e hecB 34,5% (19/55). Estas cepas foram submetidas ao AFLP gerando dois dendogramas. Foram identificados 19 perfis genotípicos para A. butzleri e 17 para A. cryaerophilus. Os resultados desta pesquisa apontam a presença de A. butzleri e A. cryaerophilus na fase final da distribuição de carne de frangos nos açougues. A falta de inocuidade dos alimentos de origem animal, bem como a presença de estirpes virulentas representam riscos de Saúde Pública, com especial atenção para a possibilidade de contaminação cruzada gerados por alimentos crus e utensílios de cozinha

Segmentação de imagens pela transformada imagem-floresta com faixa de restrição geodésica

Vários métodos tradicionais de segmentação de imagens, como a transformada de watershed de marcado- res e métodos de conexidade fuzzy (Relative Fuzzy Connectedness- RFC, Iterative Relative Fuzzy Connected- ness - IRFC), podem ser implementados de modo eficiente utilizando o método em grafos da Transformada Imagem-Floresta (Image Foresting Transform - IFT). No entanto, a carência de termos de regularização de fronteira em sua formulação fazem com que a borda do objeto segmentado possa ser altamente irregular. Um modo de contornar isto é por meio do uso de restrições de forma do objeto, que favoreçam formas mais regulares, como na recente restrição de convexidade geodésica em estrela (Geodesic Star Convexity - GSC). Neste trabalho, apresentamos uma nova restrição de forma, chamada de Faixa de Restrição Geodésica (Geodesic Band Constraint - GBC), que pode ser incorporada eficientemente em uma sub-classe do fra- mework de corte em grafos generalizado (Generalized Graph Cut - GGC), que inclui métodos pela IFT. É apresentada uma prova da otimalidade do novo algoritmo em termos de um mínimo global de uma função de energia sujeita às novas restrições de borda. A faixa de restrição geodésica nos ajuda a regularizar a borda dos objetos, consequentemente melhorando a segmentação de objetos com formas mais regulares, mantendo o baixo custo computacional da IFT. A GBC pode também ser usada conjuntamente com um mapa de custos pré estabelecido, baseado em um modelo de forma, de modo a direcionar a segmentação a seguir uma dada forma desejada, com grau de liberdade de escala e demais deformações controladas por um parâmetro único. Essa nova restrição também pode ser combinada com a GSC e com as restrições de polaridade de borda sem custo adicional. O método é demonstrado em imagens naturais, sintéticas e médicas, sendo estas provenientes de tomografias computadorizadas e de ressonância magnética.

Discursos sobre os gêneros discursivos: análise dos dizeres de sujeitos-professores do Ensino Fundamental I

Buscamos investigar, neste trabalho, quais são os discursos sobre os gêneros discursivos que circulam entre sujeitos-professores que atuam nos anos finais (4º e 5º anos) do Ensino Fundamental I, em escolas públicas municipais. Buscamos compreender também, a maneira como esses sujeitos (se) significam, em suas relações com a língua e a história. O interesse pela temática investigada se dá, principalmente, porque observamos, ao longo de nossos anos de experiência no magistério, que, embora os gêneros discursivos conforme definidos por Bakhtin (1997) sejam considerados pelos Parâmetros Curriculares Nacionais e por outros documentos legais que fundamentam a Educação Básica, como um dos principais objetivos do ensino de língua portuguesa, o conceito ainda desperta muitas dúvidas nos docentes. Filiamo-nos à Análise de Discurso de matriz francesa, postulada por Pêcheux (2009), área do conhecimento basicamente constituída na intersecção entre o materialismo histórico, a psicanálise e a linguística, e que considera a língua como lugar de materialização do discurso que é a língua em movimento, em uso pelos sujeitos. Na construção de nosso referencial teórico, mobilizamos, ainda, a teoria bakhtiniana dos gêneros do discurso, e as contribuições de teóricos das ciências da educação, tais como Pimenta (2012), Tardif (2002), Giroux (1997), Sacristán (1998), entre outros. O corpus da pesquisa é representado por seis entrevistas semiestruturadas, realizadas com sujeitos-professores que atuam em classes de 4º e 5º ano do ensino fundamental, em escolas de rede pública municipal. A partir da transcrição das entrevistas, foram realizados recortes que constituíram o material analisado neste estudo. As análises realizadas aqui indiciam que os sujeitos-professores entrevistados circulam por formações discursivas e ideológicas cristalizadas, no que diz respeito à relação dos estudantes com a língua. Tais docentes buscam desenvolver um trabalho pedagógico mediado pelos critérios de suposta familiaridade, utilidade ou praticidade em relação aos usos sociais que os gêneros discursivos poderiam ter para os estudantes. Além disso, as análises discursivas de alguns recortes apontam para um equívoco na compreensão do conceito bakhtiniano de gêneros discursivos, compreendidos, no cotidiano escolar, como uma classificação tipológica e estanque, excluindo a dinamicidade e a plasticidade que envolvem o conceito, conforme proposto por Bakhtin (1997). A cristalização da definição do conceito de gêneros discursivos, na dimensão tipológica, favorece o cerceamento da prática pedagógica, colaborando para a difusão de práticas que visam o esgotamento dos gêneros discursivos, buscando apresentar o maior número possível de gêneros aos estudantes, sob a forma de modelos que deverão ser seguidos em suas produções textuais futuras, e que lhes serão cobrados em avaliações internas e externas.