Comportamento individual de bovinos Nelore e relações com desempenho em regime de confinamento e reprodução

Pós-graduação em Zootecnia - FCAV

Influência materna nas caracteristicas morfológicas de ovários e testículos de fetos bovinos da raça Nelore

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ácidos graxos no desempenho e nas respostas imunológicas de bovinos Nelore confinados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1 M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1 M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.

Ocorrência sorológica de Babesia bovis, Babesia bigemina e Anaplasma marginale em bovinos e bubalinos no estado do Pará, Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise bayesiana para características de carcaça avaliadas por ultrassonografia de bovinos da raça Nelore Mocho, criados em bioma Cerrado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização molecular de bovinos de origem Bos indicus e seus cruzados para maciez de carne

O objetivo desse trabalho foi conhecer a variabilidade genética da calpaína e seu potencial como marcador molecular para programas de melhoramento genético, visando auxiliar na seleção de genótipos superiores para maciez de carne zebuína. Para tanto foram analisados 55 animais da raça Nelore, proveniente de outros projetos de pesquisa conduzidos pela equipe técnica do Laboratório de Genética do Instituto de Zootecnia de Nova Odessa. Os animais foram abatidos ao atingirem o acabamento de 4 mm de espessura de gordura e a amostra de carne foi coletada entre a 12ª e 13ª costelas no músculo Longissimus dorsi. A maciez de carne foi avaliada empregando-se a técnica de Warner Bratzler Shear Force em 0 e 14 dias de maturação. O DNA foi extraído a partir das amostras de carne, em seguida foi quantificado e diluído para ser amplificado por PCR. Três polimorfismos foram investigados pelas técnicas de PCR-RFLP e PCR- SSCP, localizados no exon 9, exon 14 e intron 17 do gene calpaína, sendo denominados CPN316, CAPN530, CAPN4751, respectivamente. Os resultados da análise de associação entre os dados de força de cisalhamento (FC) e marcadores moleculares revelaram efeito significativo apenas para o marcador CAPN4751. O alelo C, considerado favorável para maciez de carne, apresentou relação significativa (P>0,05) com valores menores de FC, sugerindo o seu emprego na seleção de genótipos superiores para maciez de carne de bovinos da raça Nelore

Estudo de associação entre polimorfismos de base única com características de eficiência de conversão, consumo e desempenho em bovinos da raça Nelore

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Suplementação no período das águas de bovinos e bubalinos recriados em pastagem de capim-xaraés (Urochloa brizantha Syn. Brachiaria brizantha cv. Xaraés)

Pós-graduação em Zootecnia - FMVZ

Distribuição genômica e antigênica do BHV5 em encéfalos bovinos com meningoencefalite não supurativa: casos clínicos

Pós-graduação em Ciência Animal - FMVA

Expressão gênica de TLR-2, TLR-4, HMGB1 E VEGF em úlceras abomasais em bovinos de corte

Pós-graduação em Ciência Animal - FMVA

Predição genômica para o peso ao sobreano via norma de reação em bovinos de corte

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Correlações entre escores visuais e características produtivas em prova de ganho de peso de bovinos da raça Nelore Mocha

The objective was evaluate the phenotypic correlations among visual scores, performance traits and scrotal circumference of the polled Nelore cattle performance tests. Animals of the fifth, sixth, seventh official performance test in pasture were analysed. At the end of each test period the following measures were taken: adjusted weight to 550 days, gain weight, visual score (body structure; precocity and muscle) and scrotal circumference. The musculature, precocity and body structure showed significant positive correlations (P <0.01) with gain weight, adjusted average daily weight to 550 days, scrotal circumference and chest circumference. However, only hip height showed a significant positive correlation with muscularity. Therefore, in polled Nelore cattle, regards to performance test in pasture, the visual scores show positive correlation with the performance traits and scrotal circumference, showing that visual scores is important tool in the polled Nelore Cattle performance test.

Ocorrência de Clostridium botulinum tipos C e D em criatórios de bovinos no município de Cocalinho, Vale do Araguaia, Mato Grosso

In order to assess the occurrence and distribution of spores and toxins of Clostridium botulinum types C and D in three farms in Cocalinho, at the Araguaia River valley, State of Mato Grosso, Brazil, we analyzed sediment samples from 40 water holes, soil and cattle feces, collected around water holes. Sediments were analyzed by direct method, whilst feces, soil and also sediment samples were individually analyzed by indirect method. The detection of spores and botulinum toxins in the filtered material was performed by bioassay in Swiss Webster mice strain, as well as the serum-neutralization of the positive materials for typing. Samples of cattle feces showed the largest positive rate for C. botulinum, with 25/40 (62.5%), followed by soil, 12/40 (30%), and by sediment, 13/40 (32.5%). From the 40 cattle feces samples, 25 (62.00%) were positive for Clostridium botulinum; six samples were identified as type C, other six as type D, and 13 samples were classified as CD complex. From the equal number (40) of soil samples, 12 (30%) were positive for C. botulinum; two samples were identified as type C, other three as type D, and seven samples were classified as CD complex. Regarding the 40 sediment samples, 13 (32.5%) were positive for C. botulinum; two samples were identified as type C, other three as type D, and eight samples were classified as CD complex. No botulism toxin was detected by indirect method.

Diagnóstico de situação das práticas de manejo sanitário em sistemas de produção de bovinos de corte

We interviewed 21 farmers in order to access which sanitary practices adopted in the production systems of beef cattle on farms located in São Paulo, Mato Grosso, Mato Grosso do Sul and Rondonia. The survey was conducted through a qualitative approach to the collection of data obtained through questionnaire and personal interview with the landowners. We questioned the attitudes and sanitation procedures in these current production systems such as veterinary assistance, preventive health practices and disease surveillance, knowledge of health hazards for the safe production of food, animal feed, vaccine and immunizations, registration system, measures with newborns, products used in the environment and animals, grace period, employee training, destination of the dead animals, among others. Along with the data found that only 30% of properties had veterinary care, 67% said they meet the expiration dates of the products, although it was not mentioned on the products if there were any. In only 48% of properties were veterinarians who prescribed drugs to animals. Only two of the owners confirmed buying and using expired products in animals and only 48% have received some training on the property. In this setting, it is evident that farmers should be better prepared for insertion of its products in a highly demanding and competitive market. In this context, add value to the product depends mainly on the condition of sanitation of the herds.