Fotoluminescência e mecanismo de crescimento em titanatos nanoestruturados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bromophenol blue discoloration using peroxidase immobilized on highly activated corncob powder

The aim of the present study was to evaluate the efficacy of peroxidase immobilized on corncob powder for the discoloration of dye. Peroxidase was extracted from soybean seed coat, followed by amination of the surface of the tertiary structure. The aminated peroxidase was immobilized on highly activated corncob powder and employed for the discoloration of bromophenol blue. Amination was performed with 10 or 50 mmol.L-1carbodiimide and 1 mol.L-1ethylenediamine. The amount of protein in the extract was 0.235 ± 0.011 mg.mL-1and specific peroxidase activity was 86.06 ± 1.52 µmol min-1.mg-1, using 1 mmol.L-1ABTS as substrate. Ten mmol.L-1and 50 mmol.L-1 aminated peroxidase retained 88 and 100% of the initial activity. Following covalent immobilization on a corncob powder-glyoxyl support, 10 and 50 mmol.L-1aminated peroxidase retained 74 and 86% of activity, respectively. Derivatives were used for the discoloration of 0.02 mmol.L-1bromophenol blue solution. After 30 min, 93 and 89% discoloration was achieved with the 10 mmol.L-1and 50 mmol.L-1derivatives, respectively. Moreover, these derivatives retained 60% of the catalytic properties when used three times. Peroxidase extracted from soybean seed coat immobilized on a low-cost corncob powder support exhibited improved thermal stability. Keywords: Peroxidases. Multipoint immobilization of enzymes. Aminated enzymes. Corncob powder. RESUMO Descoloração de azul de bromofenol utilizando peroxidase imobilizada em pó de sabugo de milho altamente ativado Nesta pesquisa a enzima peroxidase foi extraída do tegumento de sementes de soja, e a superfície da estrutura terciária foi aminada. A peroxidase aminada foi imobilizada em suporte pó de sabugo de milho altamente ativado e utilizado na descoloração de azul de bromofenol. A aminação da peroxidase foi realizada com carbodiimida em concentrações de 10 e 50 mmol.L-1, e 1 mol.L-1de etilenodiamina. A quantidade de proteínas no extrato foi de 0,235 ± 0,011 mg.mL-1, e a atividade específica da peroxidase foi 86,06 ± 1,52 µmol min-1.mg-1, usando 1 mmol.L-1de ABTS como substrato. A peroxidase aminada a 10 mmol.L-1reteve 88% e a aminada a 50 mmol.L-1reteve 100% da atividade inicial. As peroxidases aminadas a 10 ou 50 mmol.L-1foram covalentemente imobilizadas em suporte glioxil-pó de sabugo de milho com atividade recuperada de 74% e 86%, respectivamente. Os derivados obtidos foram utilizados na descoloração de solução de azul de bromofenol 0,02 mmol.L-1. Após 30 min 93% de descoloração foram alcançados com o derivado glioxil-pó de sabugo de milho com a peroxidase aminada 10 mmol.L-1e 89% com a aminada 50 mmol.L-1. Estes derivados mantiveram 60% das propriedades catalíticas, quando utilizado por três vezes. A peroxidase extraída do tegumento da semente de soja imobilizada em suporte de baixo custo pó de sabugo de milho apresentou melhoria na estabilidade térmica da enzima. Palavras-chave: Peroxidases. Imobilização multipontual de enzimas. Aminação de enzimas. Pó de sabugo de milho.

Avaliação in vitro da biocompatibilidade de resinas acrílicas para base de próteses e materiais reembasadores resilientes

Pós-graduação em Reabilitação Oral - FOAR

Avaliação da degradação fotoquímica de corante alimentício e lixiviação de corantes têxteis de fibras de algodão expostos a suor sintético por método cromatográficos

Pós-graduação em Química - IQ

Propriedades ópticas de materiais híbridos de sílica dopados com nanocristais de CdSe/ZnS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise dos efeitos da exposição a ambientes poluídos na morfologia e no envelhecimento precoce, de brânquias e fígado em peixes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da terapia fotodinâmica na expressão dos genes SAP5, LIP9 e PLB2 em Candida albicans

Pós-graduação em Biopatologia Bucal - ICT

Dispositivo lúdico para ensino sobre reciclagem e manutenção do meio ambiente

Descrito como o presente Privilégio de Invenção, apresenta características de brinquedo, para assim estimular e ensinar crianças sobre a importância da reciclagem de materiais, bem como a manutenção das condições do meio ambiente em geral, para tanto, sendo composto por um tabuleiro (1), um dado convencional, seis pinos, quatro discos coloridos e cartelas (2), as quais contém perguntas em uma de suas faces, sobre o tema reciclagem e reaproveitamento de material, sendo que no tabuleiro são previstas casas nas cores amarela (3), azul (4), vermelha (5) e verde (6) que, quando alcançadas pelos pinos, vira-se uma das cartelas (2) e faz-se a pergunta que consta em uma das faces da mesma, em caso de acerto, o grupo poderá jogar na próxima rodada, caso contrário perde-se a vez de jogar.

Método espectrofotométrico para determinação de etanol em gasolina e amostras comerciais de etanol

Descrito como o presente Privilégio de Invenção, refere-se a elaboração de uma metodologia analítica para a determinação quantitativa de álcool em gasolina e em amostras comerciais de Etanol, usando equipamentos de baixo custo e passível de ser executado em qualquer laboratório de rotina, para tanto, o presente método é baseado no desenvolvimento da cor azul quando o etanol reage com o molibdato de amónio em solução acidificada com ácido sulfúrico após aquecimento, reduzindo molibdênio (VI) a "azul de molibdênio", sendo a absorbância da solução resultante medida na região do visível (=670 nm) proporcional a quantidade de álcool presente.

Potential of adjuvants to reduce drift in agricultural spraying

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bioaccumulation of tributyltin by blue crabs

This study evaluated the bioaccumulation of tributyltin (TBT) by the blue crab (Callinectes sapidus). Animals were fed with contaminated food containing 30 µg g-1 of TBT expressed as Sn. The analytes were determined in the gills, hepatopancreas and muscle. Acid digestion was used in the total Sn determination, and a solid-phase extraction technique was used for the selective determination of TBT. Limits of detection of 44.6 and 4.46 ng g-1 were found for HG-ICP OES (hydride generation-inductively coupled plasma optical emission spectroscopy) and ICP-MS (ICP-mass spectrometry), respectively. The results for non-contaminated animals were below 50 ng g-1, while the animals subjected to the contaminated food showed higher tissue concentrations of Sn (until 6229 ng g-1) and TBT (until 3357 ng g-1) related to the number of exposure days. According to the results, Sn is bioaccumulated by the blue crab in higher concentrations in the hepatopancreas. For most of these animals, the results suggest that Sn is bioaccumulated as TBT.

Avaliação das características do líquido ruminal, hemogasometria, atividade pedométrica e diagnóstico de laminite subclínica em vacas leiteiras

The objectives of this study were to evaluate the characteristics of ruminal fluid, blood gas analysis, pedometer activity and suclinical laminitis occurrence, through the presence of secondary foot diseases in high production dairy cows, from a commercial herd. 200 Holstein cows originating from the same farm, located in Araçatuba/SP, Brazil, were divided into four groups, which are established from the daily milk production. Initially clinical examination of the cows was procedure, followed by sampling of rumen fluid, by esophageal tube. Fluid was evaluated for pH, color, odor, consistency, sedimentation, flotation and methylene blue reduction test. Venous blood samples were also collected for blood gas analysis, in addition to collecting data from pedometrics (number of steps) and daily milk production. Data were tabulated and submitted to correlation analysis. No animal had reported changes in rumen pH. Non-acid base imbalance were found, since the values of blood pH, PCO2, TCO2, HCO3- and BE were normal during hemogasimetric analysis. The pedometric was effective as a screening method to cows with foot diseases. It demonstrated reduction in the number of steps due to pain, correlated with lower milk production. However, the identification of foot lesions was only possible through specific digital clinical examination. The occurrence of 49.5 % of herd foot problems was due to the risk factors present in the farm. The abrasive concrete and inadequate facilities, also associated with the possible occurrence of sub-acute ruminal acidosis, were observed as risk factors. However, sub-acute ruminal acidosis was undiagnosed by the methods used. The correlation between the values of ruminal pH, and blood gas analysis pedometrics showed efficient for the early diagnosis of foot diseases and also in establishing the etiology of these diseases. Subclinical laminitis occurred primarily in cow's herd, considering the multifactorial etiology of this disease, occurrence and distribution of foot diseases diagnosed.

Cyanobacteria detection in Guarapiranga Reservoir (São Paulo State, Brazil) using Landsat TM and ETM+ images

Algae bloom is one of the major consequences of the eutrophication of aquatic systems, including algae capable of producing toxic substances. Among these are several species of cyanobacteria, also known as blue-green algae, that have the capacity to adapt themselves to changes in the water column. Thus, the horizontal distribution of cyanobacteria harmful algae blooms (CHABs) is essential, not only to the environment, but also for public health. The use of remote sensing techniques for mapping CHABs has been explored by means of bio-optical modeling of phycocyanin (PC), a unique inland waters cyanobacteria pigment. However, due to the small number of sensors with a spectral band of the PC absorption feature, it is difficult to develop semi-analytical models. This study evaluated the use of an empirical model to identify CHABs using TM and ETM+ sensors aboard Landsat 5 and 7 satellites. Five images were acquired for applying the model. Besides the images, data was also collected in the Guarapiranga Reservoir, in São Paulo Metropolitan Region, regarding the cyanobacteria cell count (cells/mL), which was used as an indicator of CHABs biomass. When model values were analyzed excluding calibration factors for temperate lakes, they showed a medium correlation (R²=0.81, p=0.036), while when the factors were included the model showed a high correlation (R²=0.96, p=0.003) to the cyanobacteria cell count. The empirical model analyzed proved useful as an important tool for policy makers, since it provided information regarding the horizontal distribution of CHABs which could not be acquired from traditional monitoring techniques.

Estudo preliminar da preferência de cor para rota e seta de manobra em mapas de sistemas de guia de rota

In-Car Navigation System and Route Guidance (RGNS), commercial or free, have adopted different graphic designs to maps which support the navigation task. This paper aims at evaluating the driver's subjective preference for colors to represent route and arrow direction in maps for these systems. A total of 54 drivers (27 males and 27 females) participated in an experiment which was performed in a parked vehicle. Two cartographic representations were produced to evaluate the route color and other two for the arrow color. The route was symbolized by black or blue color and the arrow direction by green or red hue. The cartographic representations were presented in a seven-inch navigation monitor. The results indicate that most drivers prefer the navigation route in black color. However, drivers who regularly use navigation systems have presented the tendency to prefer the route in blue hue. The direction arrow in green hue was the mostly preferred by the drivers and there was no significant correlation between different individual characteristics. This study concludes that drivers' preference was influenced by different factors, such as grouping by color, shape and proximity, subjective or conventional association, as well as by the level of contrast among the map features. Evaluating the performance of cartographic representations in route following task is recommended, in order to support the influence of graphic designs to maps in usability of route guidance systems.

Épocas e extensões do período de convivência das plantas daninhas interferindo na produtividade da cultura do milho (Zea mays)

Q field experiment was carried out at the Gralha Azul Experimental Farm/PUCPR, in the municipality of Fazenda Rio Grande-PR, to evaluate the effect of different times and coexistence extension periods of weeds interfering in corn yield. The experiment was arranged in a complete randomized complete block design and the treatments in a 4x5+2 factorial, with four replications. The treatments were as follows: four initial weed control periods (0-0, 0-7, 0-14 and 0-21 DAE) and five restarted weed control periods until the harvest (28, 42, 56, 70 and 84 DAE) and two checks, weedy and weed-free. The experiment was carried out under a no-till system. The period prior to weed interference (PBWI), the start of the critical period of weed interference (CPWI) and the infesting community were evaluated. When the initial weed control period was 0-0 day, the PPWI was at 9 DAE of corn; however, with increasing initial weed control periods (0-7, 0-14 and 0-21 DAE) the period prior to weed interference inncreased in relation to 0-0 day of the initial weed control, evidencing the existence of PPWI-S of 17, 24 and 28 DAE of corn, respectively. Thus CPWI initiates at the end of PPWI-S, the period when weed control is indispensable to prevent a significant reduction in corn yield. Coexistance with the weeds throughout the crop cycle reduced yield in 15%, compared to the weed-free check. Nine species of seven botanical families were assessed in the infesting community. Reduced density and dry mass were verified in the weeds that coexisted with corn, compared to those that grew without it, thus evidencing a suppressive effect of corn over the infesting plants.