Population dynamics of Ectatomma planidens (Hymenoptera: Formicidae) under laboratory conditions

The ant Ectatomma planidens belongs to the subfamily Ectatomminae and it is abundantly found in the Neotropical region. Five colonies of such species were studied regarding their population dynamics in the laboratory, aiming to generate life tables and determine the entropy. The life expectancy curve of the workers indicated that in the first age interval, the value of e x = 142.55 approached the mean longevity of such workers (188.23 ± 110.99 days). Upon reaching this mean longevity, there was a greater decline in the life expectancy, which tends to decrease gradually with the time. The value found for entropy was H = 0.739 (between 0.5 and 1.0), and the survival pattern of these individuals was characterized by high mortality in the first intervals and growing life expectancy as time went by.

Classification of petroleum well drilling operations with a hybrid particle swarm/ant colony algorithm

This paper describes an investigation of the hybrid PSO/ACO algorithm to classify automatically the well drilling operation stages. The method feasibility is demonstrated by its application to real mud-logging dataset. The results are compared with bio-inspired methods, and rule induction and decision tree algorithms for data mining. © 2009 Springer Berlin Heidelberg.

Ant diversity in a sugarcane culture without the use of straw burning in southeast, são paulo, Brazil

Problem statement: One of the current requirements of agroecosystem management is the maintenance of biodiversity. Manual sugarcane harvesting with the previous burning of straw has been gradually replaced by mechanical harvesting in Brazil. However, the diversity of Formicidae, which can be a natural pest controller, has not been studied in this new system yet. Approach: This study was carried out to assess the diversity of ants in an exclusively mechanically harvested sugarcane culture based on the hypothesis that species richness and abundance will increase with the deposition of straw in this culture system. Ants were sampled using pitfall traps in six sugarcane cultivars during three consecutive harvest cycles. Results: A total of 8,139 ants, distributed in 39 species, were collected. Richness, abundance and diversity differed between harvest cycles, especially in the first cycle, when the soil did not have any straw and in the two last cycles and the straw layer was about 10-15 cm thick. The communities found in the second and third cycles were similar and the maintenance of straw in the culture contributed to a greater species diversity, particularly of generalist predaceous taxa, which may contribute to the natural control of pests. Conclusion: The diversity of ants increased with straw deposition, including of taxa that may be beneficial to the sugarcane culture. However, new studies of the predatory and competition relations in this agroecosystem are necessary. © 2010 Science Publications.

Architecture for peer-to-peer databases with routing queries using ant colony algorithm and semantic support

In a peer-to-peer network, the nodes interact with each other by sharing resources, services and information. Many applications have been developed using such networks, being a class of such applications are peer-to-peer databases. The peer-to-peer databases systems allow the sharing of unstructured data, being able to integrate data from several sources, without the need of large investments, because they are used existing repositories. However, the high flexibility and dynamicity of networks the network, as well as the absence of a centralized management of information, becomes complex the process of locating information among various participants in the network. In this context, this paper presents original contributions by a proposed architecture for a routing system that uses the Ant Colony algorithm to optimize the search for desired information supported by ontologies to add semantics to shared data, enabling integration among heterogeneous databases and the while seeking to reduce the message traffic on the network without causing losses in the amount of responses, confirmed by the improve of 22.5% in this amount. © 2011 IEEE.

Division of labor in pachycondyla striata Fr. Smith, 1858 (Hymenoptera: Formicidae: Ponerinae)

Four colonies of the ant Pachycondyla striata were used to analyze the specie behavioral repertoire. Forty-six behavioral acts were recorded in laboratory. Here, we present the record the division of labor between the castes and the temporal polyethism of monomorphic workers. The queens carried out many of the behavioral traits recorded in this work however; they performed them less frequently compared to the worker. The workers activity involved chasing and feeding on fresh insects and usingthem to nourish larvae besides laying eggs in the C-posture, an activity also performed by queens, which is similar to that of wasps of the subfamily Stenogastrinae. The young workers were involved in activities of brood care, sexuate care, and nest maintenance, and the older workers were involved in defense, exploration, and foraging. © 2012 Adolfo da Silva-Melo and Edilberto Giannotti.

Presence and distribution of the endosymbiont Wolbachia among Solenopsis spp. (Hymenoptera: Formicidae) from Brazil and its evolutionary history

Wolbachia are intracellular bacteria that commonly infect arthropods. Its prevalence among ants of the genus Solenopsis is high. In the present study, the presence and distribution of these endosymbionts was examined among populations of Solenopsis spp. from Brazil. A phylogenetic analysis based on the wsp gene was conducted to infer the evolutionary history of Wolbachia infections within the populations surveyed. A high frequency of Wolbachia bacteria was observed among the genus Solenopsis, 51% of the colonies examined were infected. Incidence was higher in populations from southern Brazil. However, little genetic variability was found among different Wolbachia strains within supergroups A and B. Our findings also suggest that horizontal transmission events can occur through the social parasite S. daguerrei. © 2012 Elsevier Inc..

Intraspecific and intracolonial variation in the profile of venom alkaloids and cuticular hydrocarbons of the fire ant Solenopsis saevissima smith (Hymenoptera: Formicidae)

Fire ants are aggressive Neotropical ants that are extensively similar in general biology and morphology, making species identification difficult. Some fire ant species are top-rated pests spreading throughout the world by trade vessels. Many researchers attempted to sort between invasive and native species by using chemical characters, including patterns of venom alkaloids. The present study is the first to report intraspecific variation in some chemical characters, namely, cuticular hydrocarbons and venom alkaloids, within the Brazilian fire ant species Solenopsis saevissima and also reports on within-nest variations among members of different castes. Two different haplotypes (cryptic species) of S. saevissima were clearly identified, one presenting a predominant combination of the venom alkaloids cis- and trans-2-methyl-6-undecylpiperidine with the cuticular hydrocarbons C23, 3-Me-C23, 10-C 25:1, C25, and 3-Me-C25, and the other a predominant combination of cis- and trans-2-methyl-6-tridecenylpiperidine with predominance of 12-C25:1, C25, 11-Me-C25, 3-Me-C25, 13-C27:1, C27, and 13-Me-C 27. Intranest variations revealed that the proportions among these compounds varied sensibly among workers of different sizes, gynes, and males (no alkaloids were detected in the latter). Larva contained vestiges of the same compounds. The recorded chemical profiles are quite different from previous reports with S. saevissima samples from So Paulo. The finds thus support other recent claims that S. saevissima includes cryptic species; the study, moreover, adds the find that they can occur in the same geographical location. © 2012 Eduardo Gonalves Paterson Fox et al.

Compared morphology of the immatures of males of two urban ant species of camponotus

The immatures of males of two species of Camponotus ants (Hymenoptera: Formicidae) are described and compared by light and electron microscopy. The numbers of larval instars were determined: Camponotus rufipes Fabricius (Hymenoptera: Formicidae) have four instars; and Camponotus vittatus Forel have three. Male larvae of the two species are similar to previously described Camponotus larvae, sharing the following traits: basic shape of body and mandible, presence of 'chiloscleres', 'praesaepium' (some specimens), labial pseudopalps, and ten pairs of spiracles. However, larvae of the two species can be separated by bodily dimensions and based on their hair number and types. Worker larvae of C. vittatus previously described are extensively similar to male larvae, with only a few inconspicuous differences that may result from intraspecific variation or sexual differences.

Comparison of the ant assemblages in three phytophysionomies: Rocky field, secondary forest, and riparian forest - A case study in the state park of Ibitipoca, Brazil

Ant assemblages are almost all related with the vegetation composition and so can provide us important information for conservation strategies, which are especially relevant to an environmentally protected area. We sampled the ant fauna in three different phytophysionomies in order to verify if the composition of ant species is different among the areas, especially because one of the areas is a Rocky Field and there is little information about the ant fauna in this habitat. A total of 8730 individuals were registered and an NMDS analysis showed that the ant assemblies are different at the three phytophysionomies (Rocky Field, Riparian Forest, and Secondary Forest). This study shows that the species that compose the ant assemblies in different phytophysionomies are a reflex of the environment, supporting the hypothesis that the vegetational composition results in different compositions in the ant assembly. Vegetal composition is determinant in the formation of the litter and consequently in the occurrence of ant species that depend on this layer of organic matter for nesting and foraging. © 2012 Juliane Floriano Santos Lopes et al.

Queen lipid content and nest growth in the leaf cutting ant (Atta sexdens rubropilosa) (Hymenoptera: Formicidae)

The relationship between the queens' lipid content and nest growth (population size, biomass and nest architecture) was studied from founding up to 1 year. Nests aged 3, 4, 5, 6, 9 and 12 months were dug in the field, and their dimensions were measured. The ant nest population and fungus garden was also collected. The sample was taken to the laboratory where we counted the worker population and weighed the biomass (fungus plus offspring) and queens. Queens were separated for the determination of lipids. The lipid content in the bodies of queens decreased in the first months, then stabilized (at 4-6 months) before increasing in months 9 and 12. Nest biomass (symbiotic fungus and offspring) and worker population increased over time. The structural growth of the nests was observed by excavating around them. Initially nests (3 months old) had one chamber at an average depth of 15 cm. By 1 year, the nests had three or four deep chambers, and were about 3-4 m deep. Our study contributes to knowledge of the dynamics of the energy-reserve expenditure by queens during colony founding and colony development for up to 1 year. © 2013 Copyright Taylor and Francis Group, LLC.

A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis)

The invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods. © 2013 Elsevier Ltd.

Foraging of psilocybe basidiocarps by the leafcutting ant acromyrmex lobicornis in Santa Fé, Argentina

Background It is generally accepted that material collected by leaf-cutting ants of the genus Acromyrmex consists solely of plant matter, which is used in the nest as substrate for a symbiotic fungus providing nutrition to the ants. There is only one previous report of any leaf-cutting ant foraging directly on fungal basidiocarps. Findings Basidiocarps of Psilocybe coprophila growing on cow dung were actively collected by workers of Acromyrmex lobicornis in Santa Fé province, Argentina. During this behaviour the ants displayed typical signals of recognition and continuously recruited other foragers to the task. Basidiocarps of different stages of maturity were being transported into the nest by particular groups of workers, while other workers collected plant material. Conclusions The collection of mature basidiocarps with viable spores by leaf-cutting ants in nature adds substance to theories relating to the origin of fungiculture in these highly specialized social insects. © 2013 Masiulionis et al.

Association between Pseudonocardia symbionts and Atta leaf-cutting ants suggested by improved isolation methods

Fungus-growing ants associate with multiple symbiotic microbes, including Actinobacteria for production of antibiotics. The best studied of these bacteria are within the genus Pseudonocardia, which in most fungus-growing ants are conspicuously visible on the external cuticle of workers. However, given that fungus-growing ants in the genus Atta do not carry visible Actinobacteria on their cuticle, it is unclear if this genus engages in the symbiosis with Pseudonocardia. Here we explore whether improving culturing techniques can allow for successful isolation of Pseudonocardia from Atta cephalotes leaf-cutting ants. We obtained Pseudonocardia from 9 of 11 isolation method/colony component combinations from all 5 colonies intensively sampled. The most efficient technique was bead-beating workers in phosphate buffer solution, then plating the suspension on carboxymethylcellulose medium. Placing these strains in a fungus-growing ant-associated Pseudonocardia phylogeny revealed that while some strains grouped with clades of Pseudonocardia associated with other genera of fungus-growing ants, a large portion of the isolates fell into two novel phylogenetic clades previously not identified from this ant-microbe symbiosis. Our findings suggest that Pseudonocardia may be associated with Atta fungus-growing ants, potentially internalized, and that localizing the symbiont and exploring its role is necessary to shed further light on the association.

Influência do ambiente no desenvolvimento de colônias iniciais de formigas cortadeiras (Atta sexdens rubropilosa)

Annually, mature colonies of Atta ants produce winged males and females, where leave the colony in which they were produced to form new colonies and thus continue the species perpetuation. The foundation of Atta spp. colonies always involves high queen effort, solely responsible for growing the fungus and the care with itself and its offspring. During the foundation there is consumption of body reserves. This study investigated how the environment influences the development of newly founded colonies of leaf cutting ant, Atta sexdens rubropilosa. Therefore, it was compared field colonies collected five months after the nuptial flight and colonies maintained in the laboratory since the nuptial flight. It was analyzed the weight and size of the population (number of workers, larvae, pupae and eggs), the workers size, the volume and weight of the fungus garden and queen effort for the nest foundation (lipid content and weight of queens). In comparison to the field colonies, the laboratory colonies showed higher values in the weight and size of population, the volume and weight of the fungus garden. Measures of workers by class size did not vary between colonies from field and laboratory. The queen effort for nest foundation was higher in field colonies, which showed a lower percentage of fat and lower weight compared to laboratory colonies. These results show that the environment has a strong influence on the colonies development during the foundation, as well as on the physiological status of the queens, and therefore the survival, development and success of the colony, it is a reflection of the queen efforts and of the environment conditions.

Toxicity of hydramethylnon to the leaf-cutting ant Atta sexdens rubropilosa forel (hymenoptera: Formicidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)