Molecular and biochemical characterization of the Neurospora crassa glycogen synthase encoded by the gsn cDNA

Glycogen synthases catalyze the transfer of a glucosyl moiety from a nucleotide phosphosugar to a nascent glycogen chain via an alpha1-->4 linkage. Although many genes coding for glycogen synthases have been described, the enzymes from rabbit and yeast are the best characterized. The fungus Neurospora crassa accumulates glycogen during exponential growth, and mobilizes it at the onset of stationary phase, or when placed at high temperature or starved for carbon. Through a PCR methodology, the gsn cDNA coding for the N. crassa glycogen synthase was isolated, and the amino acid sequence of the protein was deduced. The product of the cDNA seems to be the only glycogen synthase present in N. crassa. Characterization of the gsn cDNA revealed that it codes for a 706-amino acids protein, which is very similar to mammalian and yeast glycogen synthases. Gene expression increased during exponential growth, reaching its maximal level at the end of the exponential growth phase, which is consistent with the pattern of glycogen synthase activity and glycogen level. Expression of the gsn is highly regulated at the transcriptional level. Under culture conditions that induce heat shock, conidiation, and carbon starvation, expression of the gsn gene was decreased, and glycogen synthase activity and glycogen content behaved similarly.

Activity of the Ovarian Germinal Epithelium in the Freshwater Catfish, Pimelodus maculatus (Teleostei: Ostariophysi: Siluriformes): Germline Cysts, Follicle Formation and Oocyte Development

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

E-commerce and export promotion policies for small- and medium-sized enterprises: East Asian and Latin American experiences

Includes bibliography

Las aglomeraciones productivas alrededor de la minería: el caso de la Minera Yanacocha, SA

Incluye Bibliografía

Innovation and technology policy (ITP) for catching up: a three phase life cycle framework for industrializing economies

Includes bibliography

A new approach to development banking in Jamaica

Includes bibliography

Globalization and restructuring the energy sector in Latin America

Includes bibliography

The tax regime for micro-enterprises in Cuba

Includes bibliography

Avaliação do potencial genotóxico e mutagênico de águas e sedimentos do rio Guaecá, São Sebastião - SP, após impacto de oleoduto da região

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Produção de enzimas celulolíticas pelos fungos thermoascus aurantiacus CBMAI 756, thermomyces lanuginosus, Trichoderma reesei QM9414 e Penicillium viridicatum RFC3 e aplicação na sacarificação do bagaço de cana de açucar com diferentes pré-tratamentos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A formação de joint ventures como alternativa para investimentos no setor sucroalcooleiro brasileiro

Pós-graduação em Direito - FCHS

Etnomatemática e economia solidária: o caso de um grupo de fabricação de sabão caseiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Trade, environment and development: implications for Caribbean countries

Includes bibliography

Yachting in the Eastern Caribbean: a regional overview

Includes bibliography

Olhares de “crianças” sobre gênero, sexualidade e infância

Pós-graduação em Educação - FCT