Analysis of passion fruit rinds (Passiflora edulis): isoorientin quantification by HPTLC and evaluation of antioxidant (radical scavenging) capacity

The content of isoorientin in passion fruit rinds (Passiflora edulis fo. flavicarpa O. Degener) was determined by HPTLC (high performance thin layer chromatography) with densitometric analysis. The results revealed a higher amount of isoorientin in healthy rinds of P. edulis (92.275 ± 0.610 mg L-1) than in rinds with typical symptoms of PWV (Passion fruit Woodiness Virus) infection (28.931 ± 0.346 mg L-1). The HPTLC data, allied to assays of radical scavenging activity, suggest the potential of P. edulis rinds as a natural source of flavonoids or as a possible functional food.

Mudança estrutural e mercado de trabalho no Brasil

Structural changes and labor market in Brazil. In the present article, we attempt to identify the sources of the changes in the labor schooling level in the three main sectors of the Brazilian economy: manufacturing, services and agriculture. It was verified that, despite the changes in the product and employment among sectors, mainly in the 1990s, the relative demands for qualified workers has not experimented significant changes. Moreover, in the periods in which schooling has increased more, the workers' wage has decreased more. This fact suggests that the increase in labor qualification was mainly due to the increase of this factor supply. The structural changes had contributed, in general, in a marginal and negative way to labor force level of qualification demand in all the three sectors.

Relacionismo substancial: a ontologia do comportamento à luz do behaviorismo radical

Sob a influência do pragmatismo e contextualismo pepperiano, analistas do comportamento extraíram do behaviorismo radical qualquer posição ontológica. Como resultado, há a defesa de um relacionismo radical no qual a única propriedade relevante para a existência do comportamento é a própria relação que o define. O objetivo deste ensaio é avaliar a pertinência dessa posição. Três questões guiram esse trabalho: (1) Por que a substância não é importante para o behaviorismo radical?; (2) Por que a substância é importante para o behaviorismo radical?; e (3) Qual seria, de fato, o posicionamento ontológico mais condizente com o behaviorismo radical? Argumenta-se que o relacionismo radical não reflete com acurácia a ontologia behaviorista radical e sugere-se que o relacionismo substancial seja a posição mais coerente.

Mal sem mudança - Notas iniciais sobre Angústia

O ensaio procura apontar as linhas de força que compõem o caráter dramático do narrador em Angústia. Estuda, para tanto, o movimento circular da escrita, cuja forma embaralha tempos e espaços de modo a sugerir o desconcerto. Como resultado parcial, centra-se, em termos de análise, nos primeiros fragmentos do romance a fim de compreender o refluxo das imagens que perfazem o enredo.

A educação na mudança social: lugar central, lugar secundário e lugar nenhum

Este artigo traz exemplos que sustentam a hipótese de que há uma discrepância entre o sentido geral atribuído à educação pelos enfoques sociológicos e aquele que é conferido por autoridades públicas e profissionais de organizações escolares. O sentido que tais autoridades e profissionais atribuem à educação coincide consideravelmente com as noções mais recorrentes na opinião pública, que frequentemente localiza a educação em posição central como fator de mudança social. O argumento foi elaborado para situar uma pesquisa sobre mudança educacional no Brasil, apoiada pela Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) em 2004-2005. A pesquisa dedicou-se a reportar as características das ações de reforma educacional nos anos 1990, confrontando-as com iniciativas circunscritas, abarcadas pela denominação de inovação educacional.

Estágios de mudança de comportamento e fatores associados à prática de atividade física em indivíduos com claudicação intermitente

OBJECTIVE: To analyze, in people with intermittent claudication, the frequency of individuals who are in each of stages of health behavior change to practice physical activity, and analyze the association of these stages with the walking capacity. METHODS: We recruited 150 patients with intermittent claudication treated at a tertiary center, being included those >30-year-old-individuals and who had ankle-arm index <0.90. We obtained socio-demographic information, presence of comorbidities and cardiovascular risk factors and stages of health behavior change to practice physical activity through a questionnaire, they being pre-contemplation, contemplation, preparation, action and maintenance. Moreover, the walking capacity was measured in a treadmill test (Gardner protocol). RESULTS: Most individuals were in the maintenance stage (42.7%), however, when the stages of health behavior change were categorized into active (action and maintenance) and inactive (pre-contemplation, contemplation and preparation),51.3% of the individuals were classified as inactive behavior. There was no association between stages of health behavior change, sociodemographic factors and cardiovascular risk factors. However, patients with intermittent claudication who had lower total walking distance were three times more likely to have inactive behavior. CONCLUSION: Most patients with intermittent claudication showed an inactive behavior and, in this population, lower walking capacity was associated with this behavior.

Olhares distintos sobre a noção de estabilidade e mudança no desempenho da coordenação motora grossa

Estudos sobre coordenação motora grossa (CMG) de crianças focam na descrição dos valores normativos em razão da idade e sexo. Poucos analisam a dinâmica da mudança do desempenho de cada criança em relação ao seu grupo ao longo do tempo. O objetivo do presente estudo foi estimar a estabilidade das diferenças interindividuais na mudança intraindividual da CMG ao longo de 18 meses. Participaram do estudo 120 escolares de sete anos de idade da cidade de Muzambinho - MG. Foram realizadas quatro coletas com intervalos de seis meses. A CMG foi avaliada pelas provas do KTK. Os valores médios apresentaram incrementos em todas as provas, diferindo entre gênero apenas para o equilíbrio à retaguarda. Os resultados da correlação entre o desempenho inicial e a mudança ao longo do tempo indicaram uma forte heterogeneidade. Os resultados da estabilidade se mostraram fracos, os quais foram discutidos em relação a diferentes trajetórias do desenvolvimento da CMG.

Variabilidade e mudança no clima: passado, presente e futuro

FAPESP

Irradiación radical convencional e hiperfraccionada del carcinoma de cabeza y cuello localmente avanzado : ensayos predictivos de toxicidad mediante estudios de radiosensibilidad de linfocitos in vitro y estudio de calidad de vida : tesis doctoral

Programa de Doctorado: Cáncer: Biología y Clínica

Constitutive gene expression profile segregates toxicity in locally advanced breast cancer patients treated with high-dose hyperfractionated radical radiotherapy

[EN] Breast cancer patients show a wide variation in normal tissue reactions after radiotherapy. The individual sensitivity to x-rays limits the efficiency of the therapy. Prediction of individual sensitivity to radiotherapy could help to select the radiation protocol and to improve treatment results. The aim of this study was to assess the relationship between gene expression profiles of ex vivo un-irradiated and irradiated lymphocytes and the development of toxicity due to high-dose hyperfractionated radiotherapy in patients with locally advanced breast cancer. Raw data from microarray experiments were uploaded to the Gene Expression Omnibus Database http://www.ncbi.nlm.nih.gov/geo/ (GEO accession GSE15341). We obtained a small group of 81 genes significantly regulated by radiotherapy, lumped in 50 relevant pathways. Using ANOVA and t-test statistical tools we found 20 and 26 constitutive genes (0 Gy) that segregate patients with and without acute and late toxicity, respectively. Non-supervised hierarchical clustering was used for the visualization of results. Six and 9 pathways were significantly regulated respectively. Concerning to irradiated lymphocytes (2 Gy), we founded 29 genes that separate patients with acute toxicity and without it. Those genes were gathered in 4 significant pathways. We could not identify a set of genes that segregates patients with and without late toxicity. In conclusion, we have found an association between the constitutive gene expression profile of peripheral blood lymphocytes and the development of acute and late toxicity in consecutive, unselected patients. These observations suggest the possibility of predicting normal tissue response to irradiation in high-dose non-conventional radiation therapy regimens. Prospective studies with higher number of patients are needed to validate these preliminary results.

Synthetic and mechanistic investigation of new radical processes: reaction of organic azides with group-XIII Lewis acids

Dichloroindium hydride revealed to be a valid alternative to tributyltin hydride for radical reduction of organic (alkyl, aryl, acyl, solfonyl) azides. The new approach entails mild reaction conditions and provides high yields of the corresponding amines and amides, also showing high degrees of selectivity. The system dichloroindium hydride / azides can be utilised in fivemembered ring closures of g-azidonitriles, as a new source of aminyl radicals for the attractive synthesis of interesting amidine compounds in the absence of both toxic reagents and tedious purification procedures. Allylindium dichloride seems a good substitute for dichloroindium hydride for generation of indium centred radicals under photolytic conditions, since it allows allylation of electrophilic azides (e.g. phenylsulfonyl azide) and halogen or ester δ-substituted azides, the latter through a 1,5-H transfer rearrangement mechanism. Evidences of the radical nature of the reactions mechanism were provided by ESR spectroscopy, furthermore the same technique, allowed to discover that the reaction of azides with indium trichloride and other group XIII Lewis acids, in particular gallium trichloride, gives rise to strongly coloured, persistent paramagnetic species, whose structure is consistent with the radical cation of the head-to-tail dimer of the aniline corresponding to the starting azide.

Carne y mito: representaciones de la mujer desviada en el naturalismo radical y en el degeneracionismo

Programa de doctorado: Literatura y Teoría de la Literatura

Tropospheric photochemistry of ozone, its precursors and the hydroxyl radical

Zusammenfassung Ein 3-dimensionales globales Modell der unterenAtmosphäre wurde für die Untersuchung derOzonchemie, sowie der Chemie des Hydroxylradikals (OH) undwichtiger Vorläufersubstanzen, wie reaktiverStickstoffverbindungen und Kohlenwasserstoffe, verwendet.Hierfür wurde die Behandlung vonNicht-Methan-Kohlenwasserstoffen (NMKW) hinzugefügt,was auch die Entwicklung einer vereinfachten Beschreibungihrer Chemie, sowie die Erfassung von Depositionsprozessenund Emissionen erforderte. Zur Lösung der steifengewöhnlichen Differentialgleichungen der Chemie wurdeeine schnelles Rosenbrock-Verfahren eingesetzt, das soimplementiert wurde, dass die Modell-Chemie fürzukünftige Studien leicht abgeändert werden kann. Zur Evaluierung des Modells wurde ein umfangreicherVergleich der Modellergebnisse mit Bodenmessungen, sowieFlugzeug-, Sonden- und Satelliten-Daten durchgeführt.Das Modell kann viele Aspekte der Beobachtungen derverschieden Substanzen realistisch wiedergeben. Es wurdenjedoch auch einige Diskrepanzen festgestellt, die Hinweiseauf fehlerhafte Emissionsfelder oder auf die Modell-Dynamikund auch auf fehlende Modell-Chemie liefern. Zur weiteren Untersuchung des Einflusses verschiedenerStoffgruppen wurden drei Läufe mit unterschiedlichkomplexer Chemie analysiert. Durch das Berücksichtigender NMKW wird die Verteilung mehrerer wichtiger Substanzensignifikant beeinflusst, darunter z.B. ein Anstieg desglobalen Ozons. Es wurde gezeigt, dass die biogene SubstanzIsopren etwa die Hälfte des Gesamteffekts der NMKWausmachte (mehr in den Tropen, weniger anderswo). In einer Sensitivitätsstudie wurden die Unsicherheitenbei der Modellierung von Isopren weitergehend untersucht.Dabei konnte gezeigt werden, dass die Unsicherheit beiphysikalischen Aspekten (Deposition und heterogene Prozesse)ebenso groß sein kann, wie die aus dem chemischenGasphasen-Mechanismus stammende, welche zu globalbedeutsamen Abweichungen führte. Lokal können sichnoch größere Abweichungen ergeben. Zusammenfassend kann gesagt werden, dass die numerischenStudien dieser Arbeit neue Einblicke in wichtige Aspekte derPhotochemieder Troposphäre ergaben und in Vorschläge fürweiter Studien mündeten, die die wichtigsten gefundenenUnsicherheiten weiter verringern könnten.

5-substituted triazolinyls as novel counter radicals in controlled radical polymerization

The work is devoted to synthesis of new triazolinyl stable radical derivatives with different substituents at the 5-position of the triazolinyl ring. Obtained results showed great influence of these substituents on the stability of the radical. Electron-rich aromatic substituents at this position stabilize the radical while electron-poor aromatics decrease the stability of the triazolinyl. The triazolinyl radicals synthesized were used as additives for kinetic investigations of controlled radical polymerization of styrene and methylmethacrylate (MMA). The studies performed showed that the more stable radicals provide better control for the polymerization of styrene. In the same time certain instability of the radical is required for realization of controlled polymerization of methylmethacrylate. Based on the kinetic investigations controlled radical polymerization of a variety of monomers including 4-vinylpyridine (4-VP), ethylmethacrylate (EMA), 2,2,2-trifluoroethylmethacrylate (FEMA) and n-butylmethacrylate (BMA)was successfully carried out. Polystyrene and polymethylmethacrylate macroinitiators prepared by triazolinyl mediated controlled radical polymerization were efficiently reinitiated in the presence of a variety of monomers leading to the formation of block copolymers. Using this method PS-b-P-4-VP, PMMA-b-PS, PMMA-b-PBMA, PMMA-b-PFEMA, and PMMA-b-Poly-tert-butylacrylate were successfully synthesized. The results obtained during this work showed the efficiency and flexibility of the method allowing preparation of a range of advanced macromolecular structures.