Missing pieces in the puzzle of plant microRNAs

Plant microRNAs (miRNAs) are important regulatory switches. Recent advances have revealed many regulatory layers between the two essential processes, miRNA biogenesis and function. However, how these multilayered regulatory processes ultimately control miRNA gene regulation and connects miRNAs and plant responses with the surrounding environment is still largely unknown. In this opinion article, we propose that the miRNA pathway is highly dynamic and plastic. The apparent flexibility of the miRNA pathway in plants appears to be controlled by a number recently identified proteins and poorly characterized signaling cascades. We further propose that altered miRNA accumulation can be a direct consequence of the rewiring of interactions between proteins that function in the miRNA pathway, an avenue that remains largely unexplored.

Field placement and the impact of financial stress on social work and human services students

While significant research has been undertaken exploring the pedagogical benefits of undertaking lengthy social work and human services field placements, there has been very little consideration regarding the potential financial stress involved for students. This study has addressed this knowledge gap. Research was conducted in 2014 using quantitative and qualitative methods with students, academic and professional staff from six Queensland Universities. The findings show a significant relationship between unpaid placements and financial hardship creating considerable stress for students and at times a compromised learning experience whilst on placement. The limited flexibility in the requirements of professional bodies and universities for how placements are undertaken has been identified as a key contributor to financial hardship. Addressing the complexities inherent in this issue requires a collaborative effort from multiple stakeholders and should not be regarded as a problem for students to endure and manage.

Many disciplines-common approach: Experiences in the development and delivery of an interprofessional health subject

In 2004, the Faculty of Health Sciences at La Trobe University in Victoria, Australia, introduced a new, final-year subject ‘Interdisciplinary Professional Practice’. The subject is taught to all students enrolled in the 11 allied health and human service disciplines at La Trobe University across metropolitan and rural campuses. The delivery is online to overcome timetabling barriers and to provide time and geographic flexibility. The subject is presented using an enquiry-based learning model. Students are exposed to the concepts of interdisciplinary teamwork through shared learning across professional boundaries to enable a collaborative workforce. An outline of the background development and design of this subject, and its implementation and content areas is presented. A discussion of relevant literature and an analysis of the subject evaluations and focus groups that have guided subject development to enhance student learning over eight cohorts is included.

Microcalorimetric indications for ligand binding as a function of the protein for galactoside-specific plant and avian lectins

The process cascade leading to the final accommodation of the carbohydrate ligand in the lectin’s binding site comprises enthalpic and entropic contributions of the binding partners and solvent molecules. With emphasis on lactose, N-acetyllactosamine, and thiodigalactoside as potent inhibitors of binding of galactoside-specific lectins, the question was addressed to what extent these parameters are affected as a function of the protein. The microcalorimetric study of carbohydrate association to the galectin from chicken liver (CG-16) and the agglutinin from Viscum album (VAA) revealed enthalpy–entropy compensation with evident protein type-dependent changes for N-acetyllactosamine. Reduction of the entropic penalty by differential flexibility of loops or side chains and/or solvation properties of the protein will have to be reckoned with to assign a molecular cause to protein type-dependent changes in thermodynamic parameters for lectins sharing the same monosaccharide specificity.

Surface instability of confined elastic bilayers: Theory and simulations

The surface of a soft elastic film becomes unstable and forms a self-organized undulating pattern because of adhesive interactions when it comes in contact proximity with a rigid surface. For a single film, the pattern length scale lambda, which is governed by the minimization of the elastic stored energy, gives lambda similar to 3h, where h is the film thickness. Based on a linear stability analysis and simulations of adhesion and debonding, we consider the contact instability of an elastic bilayer, which provides greater flexibility in the morphological control of interfacial instability. Unlike the case of a single film, the morphology of the contact instability patterns, debonding distance, and debonding force in a bilayer can be controlled in a nonlinear way by varying the thicknesses and shear moduli of the films. Interestingly, the pattern wavelength in a bilayer can be greatly increased or decreased compared to a single film when the adhesive contact is formed by the stiffer or the softer of the two films, respectively. In particular, lambda as small as 0.5h can be obtained. This indicates a new strategy for pattern miniaturization in elastic contact lithography.

Huumeriippuvuudesta toipuminen Nimettömien Narkomaanien toveriseurassa

The subjects of this study are Narcotics Anonymous (NA), a non-profit, peer-support-based fellowship, its recovery programme, and the former drug addicts who consider themselves members of the fellowship. The study data consist of episodic interviews (n=24) and questionnaires (n=212). In the collection of questionnaire data, survey research methods had to be applied judiciously. This study analyses NA members background and their substance abuse and treatment history, as well as factors that have contributed to or hindered their bonding with NA. A recovery model is presented that stems from NA s written and oral tradition, and which has been conceptualised into NA s recovery theory. At its simplest, NA s recovery theory can be described in two sentences: 1) There are drug dependent addicts who have an addiction disease. 2) Through an NA way of life, recovery is possible. In this study, addiction and addiction disease are described through recovery stories shared at NA. It also describes how the way of life offered by NA supports recovery from drug addiction, the way of life which recovering addicts have adopted, and how they have done so. The study also presents results that, based on the study data, emerge from participation in the NA programme, and describes how the NA recovery theory works in practice, i.e. how NA members utilise the tools provided by the fellowship and how the lives of recovering addicts change during their membership. Furthermore, this study also discusses criticism of NA. According to the study, NA affects the lives of recovering drug addicts in a number of ways. People of different ages and with a variety of personal, treatment and drug abuse histories seem to benefit from membership of NA. Viewed from the outside, NA may appear as strictly normative, but in practice each member can adapt the programme in a way that suits him/her best. Indeed, flexibility is one of the strengths of NA, but without more extensive knowledge of the fellowship, it is possible that the norms reflected in NA texts or the fanaticism of individual NA members may drive some people away. Due to the increasing number of NA members, the association is also able to provide more alternatives. This study confirms the view that peer support is important, as well as the fact that an official treatment system is required in parallel with peer support activities. NA can never fully replace professional support, neither should it be left with sole responsibility for recovering addicts. Keywords: Narcotics Anonymous, peer support, recovery study, recovery, substance addiction, drug treatment, drugs, explorative research

HALLINNONTASOJEN VÄLINEN DIALOGI JA ERITYISOPETUKSEN MUUTOS BYROKRAATTISENA INSTITUUTIONA : - Kymppikuntien loppuraportin ja Erityisopetuksen strategian sisällönanalyyttinen vertailu

The rise of Special education numbers in Finland has caused a situation where Finland s ten largest LEA s so called kymppikunnat (ten communes) have expressed their growing concern of organizing the special education in the current institutional settings. The LEA s started the conversation of redefining special education system in 2004. Their aim was to target the governments attention to the problematics of special education. By the request of the Ministry of Education the LEA s prepared a final report concerning the central questions in the Finnish special education system. On the basis of the LEA s survey it became even clearer that the legislation, funding system and curriculum are tightly linked together. The following LEA s took part into the writing process Espoo, Helsinki, Jyväskylä, Kuopio, Lahti, Lappeenranta, Tampere, Turku and Vantaa. The report was hand over to the Ministry of Education at 18.8.2006. After the delivery the Ministry organized special education development group meetings 17 times in the year 2007. The result of the LEA s report and the development meetings was a new Special Education Strategy 2007. I am observing the dialogue between administrational levels in governmental institutions change process. The research is a content analysis where I compare the Erityistä tukea tarvitsevan oppilaan opetuksen järjestämisen uudistaminen osana yhtenäistä perusopetusta- kohti laatua ja joustavuutta (The renewal of the organization of teaching for student with special educational needs as part of unified education for all - towards quality and flexibility) document to Erityisopetuksen strategia (Special education strategy) document. My aim was to find out how much of their own interests have the LEA s been able to integrate into the official governmental documentation. The data has been organized and analyzed quantitatively with Macros created as additional parts in Microsoft Excel software. The document material has also been arranged manually on sentence based categorization into an Excel matrix. The results have been theoretically viewed from the special education reform dialogue perspective, and from the angle of the change process of a bureaucratic institution. My target has been to provide a new viewpoint to the change of special education system as a bureaucratic institution. The education system has traditionally been understood as a machine bureaucracy. By the review provided in my pro gradu analysis it seems however that the administrational system in special education is more of a postmodern network bureaucracy than machine bureaucracy. The system appears to be constructed by overlapping, crossing and complex networks where things are been decided. These kinds of networks are called "governance networks . It seems that the governmental administrational - and politic levels, the third sector actors and other society s operators are mixed in decision making.

Control aspects in proportionality Markov manpower systems

In this paper, the control aspects of a hierarchical organization under the influence of "proportionality" policies are analyzed. Proportionality policies are those that restrict the recruitment to every level of the hierarchy (except the bottom most level or base level) to be in strict proportion to the promotions into that level. Both long term and short term control analysis have been discussed. In long term control the specific roles of the parameters of the system with regard to control of the shape and size of the system have been analyzed and yield suitable control strategies. In short term control, the attainability of a target or goal structure within a specific time from a given initial structure has been analyzed and yields the required recruitment strategies. The theoretical analyses have been illustrated with computational examples and also with real world data. The control of such proportionality systems is then compared with that of the general systems (which do not follow such policies) with some significant conclusions. The control relations of such proportionality systems are found to be simpler and more practically feasible than those of general Markov systems, which do not have such restrictions. Such proportionality systems thus not only retain and match the flexibility of general Markov systems but also have the added advantage of simpler and more practically feasible controls. The proportionality policies hence act as an alternative and more practicably feasible means of control. (C) 2004 Elsevier Inc. All rights reserved.

An agent based adaptive bandwidth allocation scheme for multimedia applications

Bandwidth allocation for multimedia applications in case of network congestion and failure poses technical challenges due to bursty and delay sensitive nature of the applications. The growth of multimedia services on Internet and the development of agent technology have made us to investigate new techniques for resolving the bandwidth issues in multimedia communications. Agent technology is emerging as a flexible promising solution for network resource management and QoS (Quality of Service) control in a distributed environment. In this paper, we propose an adaptive bandwidth allocation scheme for multimedia applications by deploying the static and mobile agents. It is a run-time allocation scheme that functions at the network nodes. This technique adaptively finds an alternate patchup route for every congested/failed link and reallocates the bandwidth for the affected multimedia applications. The designed method has been tested (analytical and simulation)with various network sizes and conditions. The results are presented to assess the performance and effectiveness of the approach. This work also demonstrates some of the benefits of the agent based schemes in providing flexibility, adaptability, software reusability, and maintainability. (C) 2004 Elsevier Inc. All rights reserved.

Comparative analysis of thermophilic and mesophilic proteins using Protein Energy Networks

Background: Thermophilic proteins sustain themselves and function at higher temperatures. Despite their structural and functional similarities with their mesophilic homologues, they show enhanced stability. Various comparative studies at genomic, protein sequence and structure levels, and experimental works highlight the different factors and dominant interacting forces contributing to this increased stability. Methods: In this comparative structure based study, we have used interaction energies between amino acids, to generate structure networks called as Protein Energy Networks (PENs). These PENs are used to compute network, sub-graph, and node specific parameters. These parameters are then compared between the thermophile-mesophile homologues. Results: The results show an increased number of clusters and low energy cliques in thermophiles as the main contributing factors for their enhanced stability. Further more, we see an increase in the number of hubs in thermophiles. We also observe no community of electrostatic cliques forming in PENs. Conclusion: In this study we were able to take an energy based network approach, to identify the factors responsible for enhanced stability of thermophiles, by comparative analysis. We were able to point out that the sub-graph parameters are the prominent contributing factors. The thermophiles have a better-packed hydrophobic core. We have also discussed how thermophiles, although increasing stability through higher connectivity retains conformational flexibility, from a cliques and communities perspective.

Comparative analysis of thermophilic and mesophilic proteins using Protein Energy Networks

Background: Thermophilic proteins sustain themselves and function at higher temperatures. Despite their structural and functional similarities with their mesophilic homologues, they show enhanced stability. Various comparative studies at genomic, protein sequence and structure levels, and experimental works highlight the different factors and dominant interacting forces contributing to this increased stability. Methods: In this comparative structure based study, we have used interaction energies between amino acids, to generate structure networks called as Protein Energy Networks (PENs). These PENs are used to compute network, sub-graph, and node specific parameters. These parameters are then compared between the thermophile-mesophile homologues. Results: The results show an increased number of clusters and low energy cliques in thermophiles as the main contributing factors for their enhanced stability. Further more, we see an increase in the number of hubs in thermophiles. We also observe no community of electrostatic cliques forming in PENs. Conclusion: In this study we were able to take an energy based network approach, to identify the factors responsible for enhanced stability of thermophiles, by comparative analysis. We were able to point out that the sub-graph parameters are the prominent contributing factors. The thermophiles have a better-packed hydrophobic core. We have also discussed how thermophiles, although increasing stability through higher connectivity retains conformational flexibility, from a cliques and communities perspective.

Main chain and segmental dynamics of semi interpenetrating based on polyisoprene and poly(methyl methacrylate)

Main chain and segmental dynamics of polyisoprene (PI) and poly(methyl methacrylate)(PMMA) chains in semi IPNs were systematically studied over a wide range of temperatures (above and below T-g of both polymers) as a function of composition, crosslink density, and molecular weight. The immiscible polymers retained most of its characteristic molecular motion; however, the semi IPN synthesis resulted in dramatic changes in the motional behavior of both polymers due to the molecular level interpenetration between two polymer chains. ESR spin probe method was found to be sensitive to the concentration changes of PMMA in semi IPNs. Low temperature spectra showed the characteristics of rigid limit spectra, and in the range of 293-373 K.complex spectra were obtained with the slow component mostly arisingout of the PMMA rich regions and fast component from the PI phase. We found that the rigid PMMA chains closely interpenetrated into thehighly mobile PI network imparts motional restriction in nearby PI chains, and the highly mobile PI chains induce some degree of flexibility in highly rigid PMMA chains. Molecular level interchain mixing was found to be more efficient at a PMMA concentration of 35 wt.%. Moreover, the strong interphase formed in the above mentionedsemi IPN contributed to the large slow component in the ESR spectra at higher temperature. The shape of the spectra along with the data obtained from the simulations of spectra was correlated to the morphology of the semi IPNs. The correlation time measurement detected the motional region associated with the glass transition of PI and PMMA, and these regions were found to follow the same pattern of shifts in a-relaxation of PI and PMMA observed in DMA analysis. Activation energies associated with the T-g regions were also calculated. T-50G was found to correlate with the T-g of PMMA, and the volume of polymer segments undergoing glass transitional motion was calculated to be 1.7 nm(3).C-13 T-1 rho measurements of PMMA carbons indicate that the molecular level interactions were strong in semi IPN irrespective of the immiscible nature of polymers. The motional characteristics of H atoms attached to carbon atoms in both polymers were analyzed using 2D WISE NMR. Main relaxations of both components shifted inward, and both SEM and TEM analysis showed the development of a nanometer sized morphology in the case of highly crosslinked semi IPN. (C) 2010 Elsevier Ltd. All rights reserved.

Vanhustenhoitajan ammatillinen rooli – avain vanhusten hyvinvointiin ja hoitajien työssä jaksamiseen

Suomessa väestön rakenne on muuttumassa ja hoitoa tarvitsevien määrä nousee jatkuvasti. Vanhustenhoito on muuttunut henkisesti ja fyysisesti yhä kuormittavammaksi ja vanhustenhoidon imago huonoksi. Tavoitteita asetetaan, mutta resurssit näiden saavuttamiseen ovat tiukassa. Tutkimuksen keskiössä olivat vanhustenhoitajien kokemukset ammatillisesta roolista. Tutkimuksen teoreettisina tukipilareina toimivat rooliteoria sekä näkemykset tunnetyöstä ja totaalisista laitoksista. Menetelmänä toimi IPA:a, jonka avulla voidaan päästä käsiksi siihen, miten tutkittavat jäsentävät maailmaansa − tässä tapauksessa ammatillista rooliaan vanhustyöntekijöinä. Tutkimuksen aineistona toimivat 8 haastattelua, jotka on kerätty vuonna 2009. Aineistosta nousi päällimmäisenä esiin hoitajan ja asukkaan välinen vuorovaikutus ja se, kuinka hoitajan on oltava ammatillisessa roolissa. Tätä vuorovaikutussuhdetta kuvattiin kaikissa kahdeksassa haastattelussa ja sitä pidettiin ammattitaidon oleellisimpana osana. Ensimmäisellä tutkimuskysymyksellä selvitettiin millaisia rooliodotuksia vanhustenhoitajat asettavat toiminnalleen. Oleellisimmat odotukset rakentuivat sen ympärille, miten vanhuksen kanssa ollaan vuorovaikutuksessa. Tilanteissa hoitajan tulisi osata oikeanlaiset tiukat, mutta toisaalta lempeät otteet, jotta vanhukset eivät passivoituisi. Nämä otteet nähtiin vanhuksen edun mukaisina, sillä ilman jämäkkää hoito-otetta vanhukset saattoivat heittäytyä täysihoitoon ja lakata pitämästä yllä päivittäisiä toimintojaan, kuten hampaiden pesua. Toinen tutkimuskysymys paneutui siihen, kokevatko vanhustenhoitajat roolinmukaisuuden tärkeäksi työssään. Lisäksi perehdyttiin siihen, miksi roolinmukaisuus koetaan tärkeänä. Haastatellut vanhustenhoitajat toivat esiin, että työstä on suoriuduttava tietyllä tavalla. Omista murheista tai mieltymyksistä huolimatta työt piti saada tehtyä. Työhön oltiin tultu vanhuksen edun vuoksi ja se oli pidettävä mielessä koko ajan. Kun hoitajat onnistuivat toimimaan rooliodotusten mukaisesti, säilyi tunnelma osastolla rauhallisena ja lääkkeitä kului vähemmän. Kolmas tutkimuskysymys pureutui siihen, voiko vanhustenhoitaja vapautua ammatillisesta roolistaan työpäivän aikana. Hoitajat kokivat, että roolista vapautuminen oli mahdollista työpäivän aikana, mutta sille annettiin tiukat rajat. Vanhusten nähden hoitajan tuli pysytellä tiukasti roolinmukaisessa odotettavassa käytöksessä. Vanhustenhoitajilla oli selkeästi erilaiset käyttäytymissäännöt niihin tilanteisiin kun oltiin osastolla potilaiden näkyvillä kuin niihin tilanteisiin joissa oltiin yksin tai kollegoiden kanssa. Tässä voidaan nähdä selkeä yhteys Goffmanin rooliteoriaan, jossa hän tuo esiin, että esityksissä on näyttämö ja takatila. Vanhainkodin tapauksessa näyttämö on osasto, jossa vanhukset ja omaiset seuraavat hoitajien toimintaa. Takatiloina sen sijaan toimivat hoitajien omat työhuoneet, taukotilat ja henkilökunnan wc, jossa työnaamio voitiin riisua hetkellisesti pois. Tärkeimmät lähteet ovat: Goffman, E. (1971). Arkielämän roolit. Oikeille jäljille rooliviidakossa ja Smith, J., Flowers, P., Larkin, M. (2009). Interpretative Phenomenological Analysis. Theory, method and research.

Sukupuolen rakentuminen väkivaltakertomuksissa

Tutkielmassa tarkasteltiin sukupuolen sosiaalista rakentumista väkivaltaa käsittelevissä kertomuksissa. Aineistona oli 31 lehti-ilmoituksella kerättyä väkivaltakertomusta, joissa väkivallasta kirjoitettiin sekä väkivallan uhrin että tekijän positiosta käsin. Tarkoituksena oli tutkia, millä tavoin väkivaltaa konstruoidaan kertomuksissa ja miten tämä yhdistyy kirjoittajien sukupuolen suorittamiseen. Analyysimenetelmänä oli diskurssianalyysi, jossa sovellettiin sekä poststrukturalistisen diskurssianalyysin että diskursiivisen psykologian lähestymistapaa. Aineistosta oli löydettävissä yhteensä yhdeksän erilaista väkivaltakonstruktiota. Miehen toiseen mieheen kohdistaman väkivallan kontekstissa väkivaltaa konstruoitiin kolmessa konstruktiossa oikeutetuksi ja kahdessa paheksuttavaksi. Parisuhdeväkivallan ja perheessä lapsiin kohdistuvan väkivallan kontekstissa väkivalta näyttäytyi joko väkivallan tekijään liittyvänä, valta-aseman vahvistamisena tai osapuolten vuorovaikutukseen liittyvänä. Tämän lisäksi kaikissa konteksteissa väkivaltaa konstruoitiin toisinaan väkivallan tekijän ulkoisista syistä aiheutuvaksi. Sukupuolen suorittaminen käsitettiin tutkielmassa asemointipyrkimyksinä sukupuolen mukaisiin subjektipositioihin. Kirjoittajien katsottiin kohtaavan väkivaltaan ja sukupuoleen liittyviä diskursiivisia ristiriitoja, joita he pyrkivät neuvottelemaan käyttäen erilaisia neuvottelustrategioita. Väkivaltaan ja maskuliinisuuteen liittyviä diskursiivisia ristiriitoja pyrittiin neuvottelemaan sosiaalisen paheksunnan välttämiseen ja maskuliinisuuden todistamiseen käytetyillä neuvottelustrategioilla. Uhriuteen ja feminiinisyyteen liittyviä diskursiivisia ristiriitoja neuvoteltiin uhriuden todistamiseen sekä sille vastakohtaisesti uhriuden välttelyyn käytetyillä neuvottelustrategioilla, joiden tulkittiin liittyvän pyrkimyksiin asemoitua vahvan naisen positioon. Keskeisinä neuvottelustrategioina olivat erilaiset eronteot, esimerkiksi oikeutetun ja paheksuttavan väkivallan välille, uhrin ja tekijän välille sekä itsen välille ennen ja nyt. Tärkeimmät tutkielmassa käytetyt lähteet olivat: Burr, Vivien (1995): An Introduction to Social Constructionism; Butler, Judith (1990): Gender Trouble. Feminism and the Subversion of Identity; Harré, Rom & van Langenhove, Luk (toim.) (1999): Positioning Theory: Moral Contexts of Intentional Action; Hearn, Jeff (1998): The Violences of Men. How Men Talk about and How Agencies Respond to Men’s Violence to Women; Jokinen, Arto (2000): Panssaroitu maskuliinisuus. Mies, väkivalta ja kulttuuri; Keskinen, Suvi (2005): Perheammattilaiset ja väkivaltatyön ristiriidat; Ronkainen, Suvi & Näre, Sari (toim.) (2008): Paljastettu Intiimi. Sukupuolistuneen väkivallan dynamiikka; Wetherell, Margaret (1998): Positioning and Interpretative Repertoires: Conversation Analysis and Post-Structuralism in Dialogue. Avainsanat – Nyckelord – Keywords sukupuolen rakentuminen, väkivalta, subjektipositiot, diskurssianalyysi, diskurssit

Analysis of multiple crystal forms of Bacillus subtilis BacB suggests a role for a metal ion as a nucleant for crystallization

Bacillus subtilis BacB is an oxidase that is involved in the production of the antibiotic bacilysin. This protein contains two double-stranded beta-helix (cupin) domains fused in a compact arrangement. BacB crystallizes in three crystal forms under similar crystallization conditions. An interesting observation was that a slight perturbation of the crystallization droplet resulted in the nucleation of a different crystal form. An X-ray absorption scan of BacB suggested the presence of cobalt and iron in the crystal. Here, a comparative analysis of the different crystal forms of BacB is presented in an effort to identify the basis for the different lattices. It is noted that metal ions mediating interactions across the asymmetric unit dominate the different packing arrangements. Furthermore, a normalized B-factor analysis of all the crystal structures suggests that the solvent-exposed metal ions decrease the flexibility of a loop segment, perhaps influencing the choice of crystal form. The residues coordinating the surface metal ion are similar in the triclinic and monoclinic crystal forms. The coordinating ligands for the corresponding metal ion in the tetragonal crystal form are different, leading to a tighter packing arrangement. Although BacB is a monomer in solution, a dimer of BacB serves as a template on which higher order symmetrical arrangements are formed. The different crystal forms of BacB thus provide experimental evidence for metal-ion-mediated lattice formation and crystal packing.