795 resultados para WOS request protocol


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Aims: This study aims to investigate the influence of physical training on the immune system of diabetic rats. Materials and Methods: Adult male Wistar rats were distributed into Sedentary Control (SC), Trained Control (TC), Sedentary Diabetic (SD) and Trained Diabetic (TD) groups were used. Diabetes was induced by alloxan (32 mg/bw-i.v.). Training protocol consisted of swimming, at 32 18C, one hour/day, five days/week, supporting an overload equivalent to 5 of the body weight, during four weeks. At the end of the experiment the rats were sacrificed by decapitation and blood samples were collected for glucose, insulin, albumin, hematocrit determinations, total and differential leukocyte counting. Additionally, liver samples for glycogen analyses were obtained. Results: The results were analyzed by one way at a significance level of 5. Diabetes reduced blood insulin, liver glycogen stores and increased blood glucose and neutrophil count. Physical training restored glycemia, liver glycogen levels, neutrophils and lymphocytes count in diabetic rats. Conclusions: In summary, physical training was able to improve metabolic and immunological aspects in the experimental diabetic rats.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The objective of this study was to compare the power corresponding to maximal lactate steady state determined through continuous (MLSSC) and intermittent protocol with active recovery (MLSSI). Ten trained male cyclists (25 +/- 4 yr., 72.5 +/- 10.6 kg, 178.5 +/- 4.0 cm), performed the following tests on a cycle ergometer: (1) incremental test until voluntary exhaustion to determine the maximal power (Pmax); (2) two to five constant workload tests to determine MLSSC, and; 3) two to three constant workload tests to determine MLSSI, consisting on eight repetitions of four minutes interspersed by two minutes of recovery at 50% Pmax. The MLSSC (273.2 +/- 21.4 W) was significantly lower than MLSSI (300.5 +/- 23.9 W). With base on these data, it can be verified that the intermittent exercise mode utilized in this study, allows an increase of 10% approximately, in the exercise intensity corresponding to MLSS.

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The aim of this study was to analyze the electromyographic signal of the rectus femuris (RF), vastus lateralis (VL), biceps femuris (BF), tibialis anterior (TA), and gastrocnemius lateralis (GL) muscles of seven male volunteers, during different running velocities; and determine and compare the EMG(FT) (electromyographic fatigue threshold), LT (lactate threshold) and AT (anaerobic threshold). The running protocol started with a velocity of 10 km.h(-1) and each three minutes elapsed occurred an increments of 1 km.h(-1) until the exhaustion, with an 30 second interval between each velocity increment to collect 25 mu l of blood. The increase of RMS values was verified in the RF in the velocities of 12 km.h(-1) to 14 km.h(-1). The EMG(FT) showed similarity between the muscles and in the indexes were found the following significant differences: AT > LT; EMG(FT) RF and EMG(FT) VL < AT and EMG(FT) VL and EMG(FT) BF > LT. These data confirmed the viability of using the EMGFT as a possible indicator to analyze the muscle fatigue.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Background: Ageing is characterized by a number of physical changes that contribute to a decline in the ability to perform daily tasks. Stretching has been proposed to reduce hip flexion contracture and increase hip and pelvis range of motion, thus improving gait performance. Objective: The purpose of this study was to determine whether a supervised stretching program designed to improve the range of motion of the lower limbs alters gait kinematics in older adults. Methods: Twenty healthy older adult women (65.9 +/- 4.2 years old and BMI 24.9 +/- 3.5) were divided into 2 groups. The experimental group undertook 12 sessions of stretching exercises, whereas the control group did not engage in any physical activity. Gait performance was assessed at the beginning of the experiment and after the 4-week intervention period. Results: Those in the experimental group showed increased step length, higher velocity and reduced double support time after training. In addition, participants involved in the stretching program showed greater anterior and lateral pelvis tilt and also greater rotation (p < 0.05). Conclusions: Based on our results, we can suggest that a supervised stretching program is effective to alter a number of gait variables. Moreover, after the stretching protocol, aged participants displayed gait parameters which were similar to those reported in young healthy adults. Therefore, stretching can be used as an effective means to improve range of motion and reverse some age-related changes that influence gait performance. Copyright (C) 2009 S. Karger AG, Basel

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The present study investigated the effects of moderate physical training on some of the parameters in the GH-IGF axis in experimental diabetic rats. Male Wistar rats were allocated into the following groups: sedentary control, trained control, sedentary diabetic, trained diabetic. Diabetes was induced by alloxan (32 mg/kg, b.w. iv). The physical training protocol consisted of 1 h swimming session/day, 5 days/week for 8 weeks supporting a load corresponding to 90% of maximal lactate steady state. After the experimental period, blood was collected to measure serum glucose, insulin, triglycerides, albumin, insulin-like growth factors-I (IGF-I), and growth hormone (GH). Pituitary gland was removed for GH quantification. Diabetes increased blood glucose and triglycerides and decreased insulin, IGF-I, serum and pituitary GH. Physical training decreased glucose and triglycerides, and also counteracted the reduction of serum IGF-I in diabetic rats. In conclusion, physical training recovered serum IGF-I showing no alteration of serum or pituitary GH levels.