Anti-inflammatory mechanisms of the annexin A1 protein and its mimetic peptide Ac2-26 in models of ocular inflammation in vivo and in vitro

Annexin A1 (AnxA1) is a protein that displays potent anti-inflammatory properties, but its expression in eye tissue and its role in ocular inflammatory diseases have not been well studied. We investigated the mechanism of action and potential uses of AnxA1 and its mimetic peptide (Ac2-26) in the endotoxin-induced uveitis (EIU) rodent model and in human ARPE-19 cells activated by LPS. In rats, analysis of untreated EIU after 24 and 48 h or EIU treated with topical applications or with a single s.c. injection of Ac2-26 revealed the anti-inflammatory actions of Ac2-26 on leukocyte infiltration and on the release of inflammatory mediators; the systemic administration of Boc2, a formylated peptide receptor (fpr) antagonist, abrogated the peptide's protective effects. Moreover, AnxA1-/- mice exhibited exacerbated EIU compared with wild-type animals. Immunohistochemical studies of ocular tissue showed a specific AnxA1 posttranslational modification in EIU and indicated that the fpr2 receptor mediated the anti-inflammatory actions of AnxA1. In vitro studies confirmed the roles of AnxA1 and fpr2 and the protective effects of Ac2-26 on the release of chemical mediators in ARPE-19 cells. Molecular analysis of NF-κB translocation and IL-6, IL-8, and cyclooxygenase-2 gene expression indicated that the protective effects of AnxA1 occur independently of the NF-κB signaling pathway and possibly in a posttranscriptional manner. Together, our data highlight the role of AnxA1 in ocular inflammation, especially uveitis, and suggest the use of AnxA1 or its mimetic peptide Ac2-26 as a therapeutic approach. Copyright © 2013 by The American Association of Immunologists, Inc.

Report of the Caribbean subregional meetings on maritime search and rescue

Informe de las reuniones subregionales sobre coordinacion de los servicios de busqueda y salvamento maritimo, convocadas por la Organizacion Maritima Internacional y CEPAL: 1) Reunion del Caribe Sur-Central (Kingston, Jamaica, 6-8 octubre de 1982); 2) Reunion del Caribe Oeste (Ciudad de Mexico, 13-15 octubre de 1982); y 3) Reunion del Caribe Sur (Puerto Espana, 8-11 noviembre de 1982).

Proposed work programme of the Economic Commission for Latin America and the Caribbean Subregional Headquarters for the Caribbean for the 1992-1993 biennium = Projet de programme de travail du siege sous-régional pour les Caraïbes de la CEPAL: exercice biennal 1992-1993

Contiene la propuesta del programa de trabajo de la CEPAL, Puerto España, para el bienio 1992-1993, con las principales áreas de trabajo y actividades dentro del subprograma integración económica y cooperación entre los países del Caribe.

Puerto Rico: planning board, information management strategy four-year" investment plan project"

Includes bibliography

Pressão intra-ocular, condições endoteliais e prostaglandina E2 no humor aquoso de cães com catarata senil madura ou hipermatura

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Alterações clínicas, protéicas do humor aquoso, sorológicas e histopatológicas em olhos de cães (Canis familiaris) (Linnaeus, 1758), infectados experimentalmente, por via conjuntival, com Leishmania (Leishmania) chagasi (Cunha & Chagas, 1937)

Pós-graduação em Cirurgia Veterinária - FCAV

Trade and investment flows between the Caribbean and the rest of the hemisphere in the context of the FTAA

Includes bibliography

As formas do humor. Copi: um caso argentino

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evaluaciones regionales de la Estrategia Internacional del Desarrollo. Quito, Ecuador, 1973. Chaguaramas, Trinidad y Tobago, 1975 = Regional appraisals of the International Development Strategy. Quito, Ecuador, 1973. Chaguaramas, Trinidad and Tobago, 1975

Evaluación de Quito. Resolución 320XV aprobada por la CEPAL en su Decimoquinto Período de Sesiones, Quito, Ecuador, 1973. Evaluación de Chaguaramas. Resolución 347XVI aprobada por la CEPAL en su Decimosexto Período de Sesiones, Puerto España, Trinidad y Tobago, 1975

Revista Careta: um estudo sobre humor visual no Estado Novo (1937-1945)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Interferência dos fatores externos sobre os estados de humor em jovens atletas de voleibol

Pós-graduação em Ciências da Motricidade - IBRC

Integración puerto ferrocarril: Desafíos y oportunidades para América Latina

El presente boletín FAL, analiza el papel que una buena integración modal entre los recintos portuarios y el ferrocarril tiene en la competitividad portuaria.