Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

Geological Report of Summer Field Trip 1928

The object of this trip and report was to familiarize the students of the Montana State School of Mines with methods of taking and mapping surface and undergound geology. All surface geology was mapped by means of plane table and alidade, and undergound work by means of Brunton compass and taps. The senior class of the Montana State School of MInes under the supervision of Dr. E.S. Perry performed the work, which covered an area in Madison County including South Boulder Creek, near Jefferson Island, the Silver Star Mining District, and the Alameda Mine, near Virginia City.

Geology of the Renova-Bone Basin and Mayflower Mine Areas

This report has been compiled from the data collected during the September, 1947, geologic field trip of the Montana School of Mines. The trip, under the direction of Dr. E. S. Perry, consisted of two weeks of field mapping and observation near Whitehall, Montana, and one week at the Montana School of Mines preparing this report.

A Study of the Transformation of a Twenty-Eight Per Cent Tin Bronze

In this investigation, attention is directed to the phases covered by a 28 per cent tin alloy. When the investigation was started, consideration was given to the possibility of making a Time - Temperature - Transformation curve for this parti­cular alloy. As the work progressed and further research was carried on, this phase of the work was abandoned.

Investigation of the Potential Effects of Epizootic Hemorrhagic Disease Virus on Iowa Cattle

Epizootic hemorrhagic disease virus (EHDV), an arthropod-borne orbivirus, causes significant mortality in white-tailed deer and can also cause disease in cattle. Objectives of this preliminary investigation were 1) to survey cattle at auction markets to determine the prevalence of anti-EHDV antibodies in Iowa cattle, 2) to determine EHDV seroprevalence in herds in which clinical EHD had been diagnosed, and 3) to determine whether EHDV is associated with stillbirths and/or congenital anomalies in calves. There was a 15% seroprevalence in auction market cattle; positive cattle were from southern, central, and western Iowa. Herds in which clinical EHD had been diagnosed had >60% seroprevalence. Viremia was detected in both clinically affected and unaffected cattle during an EHD outbreak. EHDV exposure was not consistently associated with congenital anomalies. Although additional surveillance is warranted, EHDV is unlikely to have a significant effect on the reproductive health of Iowa cattle.

Recent skin self-examination and doctor visits in relation to melanoma risk and tumour depth

BACKGROUND Little is known about the potential benefit of skin self-examination for melanoma prevention and early detection. Objectives: To determine whether skin self-examination is associated with reduced melanoma risk, self-detection of tumours, and reduced risk of deeper melanomas. METHODS We used data from a population-based case-control study (423 cases, 678 controls) to assess recent skin self-examination in relation to self-detection, melanoma risk and tumour depth ( ≤1 mm; > 1 mm). Logistic regression was used to estimate odds ratios (ORs) and confidence intervals (CIs) for associations of interest. RESULTS Skin self-examination conducted 1-11 times during a recent year was associated with a possible decrease in melanoma risk (OR 0·74; 95% CI 0·54-1·02). Melanoma risk was decreased for those who conducted skin self-examination and saw a doctor (OR 0·52; 95% CI 0·30-0·90). Among cases, those who examined their skin were twice as likely to self-detect the melanoma (OR 2·23; 95% CI 1·47-3·38), but self-detection was not associated with shallower tumours. Tumour depth was reduced for those who conducted skin self-examination 1-11 times during a recent year (OR 0·39; 95% CI 0·18-0·81), but was not influenced by seeing a doctor, or by conducting skin self-examination and seeing a doctor. CONCLUSIONS Risk of a deeper tumour and possibly risk of melanoma were reduced by skin self-examination 1-11 times annually. Melanoma risk was markedly reduced by skin self-examination coupled with a doctor visit. We cannot, however, exclude the possibility that our findings reflect bias or confounding. Additional studies are needed to elucidate the potential benefits of skin self-examination for melanoma prevention and early detection.

Validation of a paradigm to measure instrumental aggression (iap) on a sample of male adolescents with adjustment problems

Aggressive behavior can be classified in hostile and instrumental aggressions (anderson & bushman, 2002). this classification is mostly synonymously used with reactive and proactive aggression, whereas the differences between hostile and instrumental aggression lie on three dimensions, the primary goal, amount of anger and planning and calculation(bushman & anderson, 2001). although there are rating instruments and experimental paradigms to measure hostile aggression, there is no instrument to measure instrumental aggression. the following study will present an account to measure instrumental aggression with an experimental laboratory paradigm. the instrument was firstly tested on two samples of normal young adolescents (n1 = 100; amage. = 19.14; n2 = 60; amage. = 21.46). the first study revealed a strong correlation with a laboratory aggression paradigm measuring hostile aggression, but no correlations with self-reported aggression in the buss and perry questionnaire. these results were replicated in a second study, revealing an additional correlation with aggressive but not adaptive assertiveness. secondly the instrument was part of the evaluation of the reasoning and rehabilitation program r&r2 (ross, hilborn & lidell, 1984) in an institution for male adolescents with adjustment problems in switzerland. the r&r2 is a cognitive behavioral group therapy to reduce antisocial and promote prosocial cognitions and behavior. the treatment group (n= 16; rangeage = 15-17) is compared to a no treatment control group (n=24; rangeage = 17-19) preand post- treatment. further aggressive behavior was surveyed and experimentally measured. hostile rumination, aggressive and adaptive assertiveness, emotional and social competence were included in the measurement to estimate construct validity.

PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores.

The PAT family of lipid droplet proteins includes 5 members in mammals: perilipin, adipose differentiation-related protein (ADRP), tail-interacting protein of 47 kDa (TIP47), S3-12, and OXPAT. Members of this family are also present in evolutionarily distant organisms, including insects, slime molds and fungi. All PAT proteins share sequence similarity and the ability to bind intracellular lipid droplets, either constitutively or in response to metabolic stimuli, such as increased lipid flux into or out of lipid droplets. Positioned at the lipid droplet surface, PAT proteins manage access of other proteins (lipases) to the lipid esters within the lipid droplet core and can interact with cellular machinery important for lipid droplet biogenesis. Genetic variations in the gene for the best-characterized of the mammalian PAT proteins, perilipin, have been associated with metabolic phenotypes, including type 2 diabetes mellitus and obesity. In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms.

Safety of lntensive In-Home Family Workers

Violence against social workers and other helping professionals has increased. Within this context, intensive in-home family workers were asked about their safety in working with clients. If family workers stated that that they felt only somewhat safe or not safe at all, they were asked to explain where they are likely to feel unsafe. Findings include concerns about safety. The author suggests comprehensive educational curriculum be integrated in agency training for new hires and seasoned employees; also, training should be implemented to increase worker awareness of potentially violent situations and how to de-escalate and defuse potentially violent clients.

Family Preservation Journal, 2001, Volume 5, Issue 2. (Entire issue)

Entire issue (large pdf file) Articles include: What is Family Preservation and Why Does it Matter? J McCroskey Family Preservation Research: Where We've Been, Where We Should Be Going. Jane Yoo and William Meezan Safety of lntensive In-Home Family Workers. Gwendolyn D. Perry-Burney Family Preservation Services to At-Risk Families: A Macro Case Study. Charles A. Sallee and Alvin L. Sallee

Variations in Ice Rafted Detritus on Beaches in the South Shetland Islands: A Possible Climate Proxy

Raised beach ridges on Livingston Island of the South Shetland Islands display variations in both quantity and source of ice rafted detritus (IRD) received over time. Whereas the modem beach exhibits little IRD, all of which is of local origin, the next highest beach (similar to250 C-14 yr BP) has large amounts, some of which comes from as far away as the Antarctic Peninsula. Significant quantities of IRD also were deposited similar to 1750 C-14 yr BP. Both time periods coincide with generally cooler regional conditions and, at least in the case of the similar to250 yr old beach, local glacial advance. We suggest that the increases in ice rafting may reflect periods of greater glacial activity, altered ocean circulation, and/or greater iceberg preservation during the late Holocene. Limited IRD and lack of far-travelled erratics on the modem beach are both consistent with the ongoing warming trend in the Antarctic Peninsula region.