Acidovorax citruli: genetic analyses and protocol for its detection in seeds

Bacterial fruit blotch of cucurbits (BFB), caused by the seed borne Gramnegative bacterium Acidovorax citrulli is a serious threat to cucurbit industry worldwide. Since late 1980`s after devastating outbreaks in watermelon fields in southern United States, BFB has spread worldwide and has been reported in other cucurbit crops such as melon, pumpkin, cucumber and squash. To date, there is evidence for the existence of at least two genetically and pathogenically distinct populations of A. citrulli. In Brazil, the first report of BFB was in 1991, in a watermelon field in São Paulo. Although widespread in the country, BFB has been a major problem to melon production. More precisely, BFB has caused significant yield losses to melon production in northeastern Brazil, which concentrates > 90% of the country`s melon production. Despite the management efforts and the recent advances in A. citrulli research, BFB is still a continuous threat to the cucurbit industry, including seed producers, growers and transplant nurseries. To better understand the population structure of A. citrulli strains in Brazil, and to provide a basis for the integrated management of BFB, we used pulsed-field gel electrophoresis (PFGE), multilocus sequence analysis (MLSA) of housekeeping and virulence-associated genes and pathogenicity tests on different cucurbit seedlings to characterize a Brazilian population of A. citrulli strains from different hosts and regions. Additionally, we conducted for the first time a comparative analysis of the A. citrulli group I and II population at genomic level and showed that these two groups differ on their genome sizes due to the presence of eight DNA segments, which are present in group II and absent in group I genomes. We also provide the first evidence to suggest that temperature might be a driver in the ecological adaptation of A. citrulli populations under nutrient-rich or -depleted conditions. Finally, in order to improve the routine detection of A. citrulli on melon seedlots, we designed a new primer set that is able to detect the different Brazilian haplotypes, thus minimizing the risk of false-negatives on PCR-based seed health testing.

Compartimentos de K, Ca e Mg em solos e sua relação com o crescimento inicial, fisiologia e produtividade do eucalipto

Forestry has grown in a continuous and accelerated manner in Brazil, constituting a strategic activity for the generation of employment, income and tributes, favoring social and economic development of Brazilian agribusiness. The objectives of this study were: (1) evaluate the contents of K, Ca and Mg in the reserve compartments, non-interchangeable, interchangeable, available and the speed of its release, its correlations and its effects over productivity (annual average increment – AAI) of eucalyptus plantations, in forest sites cultivated in soils of the state of Rio Grande do Sul; (2) evaluate the initial growth, nutrition and physiological aspects of eucalyptus plants, cultivated with and without the addition of mineral sources of potassium (K), calcium (Ca) and magnesium (MG), in soils obtained from forest sites in the state of Rio Grande do Sul. In the first study, contents of K, Ca and Mg were evaluated in sulfuric digestion extract, boiling nitric acid, ammonium chloride, Mehlich-1 (only K), potassium chloride (Ca and Mg), as well as the release speed of these nutrients in the soil. In the second study, growth variables, nutritional aspects, photosynthetic rate (A) and transpiration rate of the plants (E) grown in distinct soils were evaluated under controlled conditions. The contents of K, Ca and Mg varied between compartments and depths in the studied soil classes, with the highest proportions found in the reserve compartment, indicating the importance of this compartment for the supplement of these nutrients at average and long terms. The great majority of K, Ca and Mg compartments presented significant correlations between each other, showing the dependence between them and the importance of evaluating the contents of these nutrients in the different compartments to adapt the nutritional management of the plants to each soil class, and to obtain continuous productions, minimizing the negative effects to the environment. Plants cultivated in soils that present larger reserves, availability and K, Ca and Mg release kinetics, presented similar height (H), stem diameter (SD) and shoot dry mass (SDM), with or without fertilization with K, Ca and Mg. The plants presented higher leaf content and accumulation of K in all soils fertilized with K, Ca and Mg.

Avaliação da performance de eletrodos de filmes finos de Ti/TiO 2 com diferentes tamanhos de nanopartículas na oxidação fotoeletrocatalítica de Índigo Carmim

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação dos efeitos das atividades antrópicas na bacia hidrográfica do Córrego do Ipê, município de Ilha Solteira-SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de uso de SIG no sistema de coleta de resíduos sólidos domiciliares em uma cidade de pequeno porte

Pós-graduação em Engenharia Civil - FEIS

A interface da urgência em saúde bucal no SUS: o caso de um Pronto Socorro, no município de São Paulo, 2006

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do substrato no cultivo e na composição química do cogumelo Pleurotus florida

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Potencial de linhagens experimentais de milho, oriundas de populações braquíticas, para produção de híbridos

Pós-graduação em Agronomia - FEIS

Agências reguladoras: participação cidadã no seu controle e gestao como forma de combate à captura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação reológica de nanoemulsões para sistema transdérmico de liberação de fármacos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação da teoria das restrições no gerenciamento da cadeia de suprimentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Toxicidade de produtos sintetizados a partir de extratos de plantas e do bistrifluron para o cupim subterrâneo Coptotermes gestroi (Wasmann, 1896) (Isoptera; Rhinotermitidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A teoria do funcional da densidade na caracterização de fases intermetálicas ordenadas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Compactação de um cambissolo háplico com o tráfego de um trator skidder no arraste de pinus elliottii

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Milho e feijão-caupi cultivados em faixas na safrinha

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)