Synthesis and NMR characterization of aliphatic-aromatic copolyesters by reaction of poly(ethylene terephthalate) post-consumer and poly(ethylene adipate)

An aliphatic-aromatic copolyester of poly(ethylene terephthalate), PET, and poly(ethylene adipate), PEA, PET-co-PEA, was synthesized by the high temperature melt reaction of post-consumer PET and PEA. As observed by NMR spectroscopy, the reaction yielded random copolyesters in a few minutes through ester-interchange reactions, even without added catalyst. The copolyesters obtained in the presence of a catalyst presented higher intrinsic viscosity than that obtained without the addition of catalyst, due to simultaneous polycondensation and ester-interchange reactions. The structure of the aliphatic-aromatic copolyesters obtained in different PET/PEA ratio is random as observed by NMR analysis.

Predictors of psychiatric hospitalization during 6 months of maintenance treatment with olanzapine long-acting injection: post hoc analysis of a randomized, double-blind study.

BACKGROUND: Hospitalization is a costly and distressing event associated with relapse during schizophrenia treatment. No information is available on the predictors of psychiatric hospitalization during maintenance treatment with olanzapine long-acting injection (olanzapine-LAI) or how the risk of hospitalization differs between olanzapine-LAI and oral olanzapine. This study aimed to identify the predictors of psychiatric hospitalization during maintenance treatment with olanzapine-LAI and assessed four parameters: hospitalization prevalence, incidence rate, duration, and the time to first hospitalization. Olanzapine-LAI was also compared with a sub-therapeutic dose of olanzapine-LAI and with oral olanzapine. METHODS: This was a post hoc exploratory analysis of data from a randomized, double-blind study comparing the safety and efficacy of olanzapine-LAI (pooled active depot groups: 405 mg/4 weeks, 300 mg/2 weeks, and 150 mg/2 weeks) with oral olanzapine and sub-therapeutic olanzapine-LAI (45 mg/4 weeks) during 6 months' maintenance treatment of clinically stable schizophrenia outpatients (n=1064). The four psychiatric hospitalization parameters were analyzed for each treatment group. Within the olanzapine-LAI group, patients with and without hospitalization were compared on baseline characteristics. Logistic regression and Cox's proportional hazards models were used to identify the best predictors of hospitalization. Comparisons between the treatment groups employed descriptive statistics, the Kaplan-Meier estimator and Cox's proportional hazards models. RESULTS: Psychiatric hospitalization was best predicted by suicide threats in the 12 months before baseline and by prior hospitalization. Compared with sub-therapeutic olanzapine-LAI, olanzapine-LAI was associated with a significantly lower hospitalization rate (5.2% versus 11.1%, p < 0.01), a lower mean number of hospitalizations (0.1 versus 0.2, p = 0.01), a shorter mean duration of hospitalization (1.5 days versus 2.9 days, p < 0.01), and a similar median time to first hospitalization (35 versus 60 days, p = 0.48). Olanzapine-LAI did not differ significantly from oral olanzapine on the studied hospitalization parameters. CONCLUSIONS: In clinically stable schizophrenia outpatients receiving olanzapine-LAI maintenance treatment, psychiatric hospitalization was best predicted by a history of suicide threats and prior psychiatric hospitalization. Olanzapine-LAI was associated with a significantly lower incidence of psychiatric hospitalization and shorter duration of hospitalization compared with sub-therapeutic olanzapine-LAI. Olanzapine-LAI did not differ significantly from oral olanzapine on hospitalization parameters.

Ralstonia solanacearum, a widespread bacterial plant pathogen in the post-genomic era

Ralstonia solanacearum is a soil-borne bacterium causing the widespread disease known as bacterial wilt. Ralstonia solanacearum is also the causal agent of Moko disease of banana and brown rot of potato. Since the last R. solanacearum pathogen profile was published 10 years ago, studies concerning this plant pathogen have taken a genomic and post-genomic direction. This was pioneered by the first sequenced and annotated genome for a major plant bacterial pathogen and followed by many more genomes in subsequent years. All molecular features studied now have a genomic flavour. In the future, this will help in connecting the classical field of pathology and diversity studies with the gene content of specific strains. In this review, we summarize the recent research on this bacterial pathogen, including strain classification, host range, pathogenicity determinants, regulation of virulence genes, type III effector repertoire, effector-triggered immunity, plant signalling in response to R. solanacearum, as well as a review of different new pathosystems.

Evolución de los conocimientos acerca de los trastornos del comportamiento alimentario, a través de una intervención educativa en estudiantes de enseñanza post-obligatoria

Introducción: A pesar de ser considerados eventos infrecuentes en comparación a otros estados patológicos relacionados con la alimentación, en los últimos años y en diferentes países, se constata un incremento de las tasas de incidencia y prevalencia de los Tras - tornos del Comportamiento Alimentario; la mayoría de estudios que analizan los factores que pueden influir en su aparición, suelen implicar a jóvenes y adolescentes de poblaciones pre-mórbidas o mórbidas, siendo menos frecuentes los realizados sobre población sana y estándar. Objetivos: Evaluar el nivel de conocimientos acerca de los Trastornos del Comportamiento Alimentario y comprobar la eficacia de una intervención educativa, en jóvenes de una población presumiblemente sana. Métodos: Estudio de intervención con evaluación pre y post intervención, a través de un cuestionario previamente validado de 10 preguntas cerradas y abiertas, en estudiantes de Fisioterapia, Educación Infantil y Hostelería. Resultados: Respondieron el cuestionario inicial 154 estu diantes, 85 universitarios y 39 de ciclo formativo en Hostelería, mayoritariamente mujeres (68%) y con una edad media de 20,8 años. Los estudiantes demostraron un nivel medio de conocimientos inicial, mayor en las mujeres y en los estudiantes universitarios que en los de formación profesional. Los factores de riesgo y las manifestaciones clínicas fueron los aspectos menos conocidos por los estudiantes. La intervención educativa realizada en los 35 estudiantes de Hostelería que participaron en el taller y respondieron el cuestionario post-intervención, consiguió elevar el nivel de conocimientos hasta una calificación media superior a la inicial y a la de los grupos en que no se realizó la intervención. Discusión y conclusiones: A pesar de que la intervención realizada resultó eficaz para mejorar el nivel de conocimientos en muchos de los participantes, los resultados del cuestionario post-intervención revelan la necesidad de educar a los jóvenes en los rasgos que definen y caracterizan un Trastorno del Compor tamiento Alimen - tario, adaptando el abordaje de los mismos a su realidad social. La inclusión de temas relacionados con la nutrición y la salud en los programas curriculares de los estudios post-obligatorios mejoraría el nivel de conocimientos entre los futuros profesionales de los ámbitos de la salud, la educación y la hostelería/restauración.

Les perspectives post-crisi

El 29 d'abril el conseller d'Empresa i Ocupació deia que l'objectiu és aconseguir que l'any 2020 el pes dels sectors industrials en el PIB català arribi al 25%. La declaració oficial és que el govern aposta per una indústria més intensiva en innovació, en talent i inserida en l'economia global. Segons el govern es passa d'una declaració d'intencions a accions concretes d'aplicació immediata [...]

Differential Regulation of c-FLIP Isoforms Through Post-translational Modifications

Cells are constantly responding to signals from the surrounding tissues and the environment. To dispose of infected and potentially dangerous cells, to ensure the optimal execution of developmental processes and to maintain tissue homeostasis, a multicellular organism needs to tightly control both the number and the quality of its cells. Apoptosis is a form of active cellular self-destruction that enables an organism to regulate its cell number by deleting damaged or potentially dangerous cells. Apoptosis can be induced by death ligands, which bind to death receptors on the cell surface. Ligation of the receptors leads to the formation of an intracellular death inducing signaling complex (DISC). One of the DISC components is caspase-8, a protease that triggers the caspase cascade and is thereby a key initiator of programmed cell death. The activation of caspase-8 is controlled by the cellular FLICE-inhibitory proteins (c-FLIPs). Consequently, sensitivity towards receptor-mediated apoptosis is determined by the amount of c-FLIP, and the c-FLIP levels are actively regulated for example during erythroid differentiation of K562 erythroleukemia cells and by hyperthermia in Jurkat leukemia cells. The aim of my thesis was to investigate how c-FLIP is regulated during these processes. We found that c-FLIP isoforms are short-lived proteins, although c-FLIPS had an even shorter half-life than c-FLIPL. In both experimental models, increased death receptor sensitivity correlated with induced ubiquitylation and consequent proteasomal degradation of c-FLIP. Furthermore, we elucidated how phosphorylation regulates the biological functions and the turnover of c-FLIP, thereby contributing to death receptor sensitivity. We mapped the first phosphorylation sites on c-FLIP and dissected how their phosphorylation affects c-FLIP. Moreover, we demonstrated that phosphorylation of serine 193, a phosphorylated residue common to all c-FLIPs, is primarily mediated by the classical PKC. Furthermore, we discovered a novel connection between the phosphorylation and ubiquitylation of c-FLIP: phosphorylation of S193 protects c-FLIP from ubiquitylation. Surprisingly, although all c-FLIP isoforms are phosphorylated on this conserved residue, the biological outcome is different for the long and short isoforms, since S193 specifically prolongs the half-lives of the short c-FLIP isoforms, but not c-FLIPL. To summarize, we show that c-FLIP proteins are modified by ubiquitylation and phosphorylation, and that the biological outcomes of these modifications are isoform-specifically determined.

Caussae litterarum restaurationem post medii aevi barbariem promoventes

Dedicatio: Gustaf Bergbom [ruots. pr.].

Tractatus pacis Cardisensis, secundum exemplum manu scriptum Russicum in Bibliotheca Auraica servatum, cum versione latina et animadversionibus

Arkit: 1 arkintunnukseton lehti, A-B4 C3.

De peccatis post mortem

Invocatio: I.N.D.O.M.

De peccatis post mortem

Invocatio: I.N.J.C. Gabriel Welonius.

Deskriptiivisten ja onomatopoeettisten verbien kääntämisestä. Tarkasteltavana Mikko Rimmisen Pussikaljaromaani ja Stefan Mosterin siitä laatima saksannos Tütenbierroman

Tämän pro gradu -tutkielman aiheena on deskriptiivisten ja onomatopoeettisten verbien kääntäminen, johon syvennytään tutkimalla Mikko Rimmisen Pussikaljaromaanissa (2004) ja sen nimellä Tütenbierroman (2007) ilmestyneessä, Stefan Mosterin laatimassa saksannoksessa esiintyviä deskriptiivisiä ja onomatopoeettisia verbejä sekä niitä läheisesti muistuttavia, tyylillisesti sävyttyneitä verbejä. Tarkoituksena on selvittää, löytyykö näille verbeille saksan kielestä yhtä ilmeikkäitä vastineita, millaisia strategioita kääntäjä on läheisten vastineiden puutteessa käyttänyt ja missä määrin omaperäiset sananvalinnat ovat säilyneet kohdetekstissä. Tutkimuksen painopiste on kääntäjän käyttämissä yleisstrategioissa. Käännösratkaisut jaotellaan pääasiassa sen mukaan, onko käytetyn vastineen merkitys konteksti huomioon ottaen lähellä alkuperäistä vai eroaako vastine keskeisiltä merkityspiirteiltään olennaisesti alkutekstin verbistä, sekä sen mukaan, ovatko sananvalinnat tekstiyhteyteen nähden odotuksenmukaisia vai päinvastoin omaperäisiä. Tarkasteltavaksi on otettu noin kymmenesosa romaanin pituudesta. Mosterin käännöksessä on usein päädytty alkutekstiä värittömämpiin sananvalintoihin, ja varsinkin deskriptiivisten verbien käännösvastineet poikkeavat usein kontekstin kannalta keskeisiltä merkityspiirteiltään alkutekstin ilmaisuista. Useimmiten verbit on kuitenkin käännetty yhdellä verbillä; selittäviin määreisiin ja täydennyksiin on turvauduttu vain harvoin. Omaperäiset sananvalinnat on onnistuttu säilyttämään hyvin. Tutkimus osoittaa, että niin deskriptiivisten, onomatopoeettisten kuin muidenkin tyylillisesti sävyttyneiden verbien kääntäminen voi olla haastavaa. Kaikissa näissä ryhmissä käännösvastineet vaihtelevat deskriptiivisistä täysin neutraaleihin verbeihin. Mosterin käännöksessä tyyliltään täysin neutraalit vastineet ovat kuitenkin harvinaisia, ja tutkittujen tapausten perusteella näyttääkin siltä, että verbeille on yleensä mahdollista löytää toimiva, sekä tyylinsä että merkityksensä suhteen tyydyttävä käännösvastine. Myös saksan kielessä on paljon deskriptiivisiä, onomatopoeettisia ja muita tyylillisesti sävyttyneitä verbejä, joskin ne ilmaisevat monesti hieman eri asioita kuin suomenkieliset.