Estudos sôbre a alimentação mineral do cafeeiro. II: absorção do superfosfato radioativo pelo cafeeiro (Coffea arabica L., var. Bourbon Amarelo) em condições de campo

In order to find out the best way to supply phosphorus to coffee plants when growing in "terra roxa misturada", a red soil with a high fixing capacity, tagged superphosphate was applied by the following procedures: (1) topdressed in a circular strip around the trees; (2) placed in the bottom of a circular furrow 15 cm deep; (3) placed in a semicircular furrow also 15 cm deep; (4) sprayed directly to the leaves. In each case 150 gms. of ordinary superphosphate tagged with H3 P32 O4 to give 5 X 10(9) c.p.m. were given to the two and half year old coffee plants. It was found that for the several treatments of the total phosphorus in the leaves the following values, on a per cent basis, came from the applied superphosphates: (1) topdressed 10.2 per cent, (2) circular furrow 2.4 per cent, (3) semicircular furrow 1.7 per cent, (4) sprayed 38.0 per cent; one can see, then, that methods (2) and (3) commonly used by the coffee planters are a very inefficient way to supply phosphorus in this type of soil. The remarkable foliar absorption was checked twice: a water culture experiment was carried out, the radiophosphorus being supplied by brushing it in the upper and lower surfaces of a given leaf; radioactivity was detected all over the plant as a result both of absorption and translocation; on the other hand, leaves collected from the sprayed trees were radioautographed; the radioautographs showed the pattern of distribution of the P32 which indicates true absorption rather than a surface contamination. In another locality, an experiment was caried out with 8 year old plants growing in "arenito de Bauru" which is a sandy soil with much less phosphorus fixing capacity. In this experiment the aim was to compare absorption of tagged superphosphate by trees growin under mulch against plants not receiving this treatment, The uptake of phosphorus was the same for both sets of plants. In both field experiments soil samples down to 15 cm in the profile were collected and its 0.2NHC1 soluble phosphorus was counted; rather significant values were observed mainly in the upper 5 cm layers.

Crescilin (suplemento de penicilina G procaína cristalina) na alimentação de leitões

The A. studied the use of "crescilin", a residual byproduct of penicillin manufacture. It has been recommended the proportion of 1 per cent and proclaimed to supply 400,000 U. O. per kg. The results obtained permited to conclude that 1 per cent of crescilin did not increase the growth of pigs.

O farelinho de trigo fermentado com Bacta-Mon e excremento de vaca na alimentação de pintos

Bacta-mon, produced by Bacta-mon S. A., São Paulo, Brazil, is recommended as a microbiological, and suggested as a supplement for animal rations. This experiment deals with this product in chicken feeding. Four lots of baby chicken received, during 6 weeks the following treatments: a control ration Rl; a ration R2 containing 10 per cent of wheat standard middlings fermented by Bacta-mon, substituting equal weight of wheat standard middlings of the control ration Rl; two rations R3 and R4, both without meat meal and containing 10 per cent of wheat standard middlings fermented respectively by Bactamon and fresh cow manure, substituting equal weight of wheat standard middlings of the control. The results may be so summarized: (1) On the basis of the weights of the chicks at 6 weeks age, we concluded that there was not any advantage in the addition of the wheat standard middlings fermented by Bacta-mon. (2) The rations R3 and R4 were considered statistically equivalents and lower the control ration Rl. (3) It seems that the main difference observed in these results may be atributed to lack of animal protein. (4) The highest mortality and the lowest consumption of feed by the lots receiving ration R3 and R4, seem to indicate, in addition, that this prejudice was due the lack of animal protein and the unpalatability of these rations.

Ocorrência de Actinomicetos com atividade proteolitica, em um solo cultivado

Among 327 strains of Actinomycetes isolated in 1958 during the four seasons, 97,85 per cent had proteolytic activity. Most of the strains wete isolated in spring time. About the organic matter no conclusions were reached, because it was mantained constant with a average value of 4.099. So the different number of the strains was a consequence of the season. We can conclude that the Latosol red-yellow soil, from where these microorganisms were isolated, has a strong proteolytic capacity, since this reaction is very strong in a maximum limit of sixteen days during the spring season. Consequently, this soil has one of the most importante caracteristics of fertility. The role of the Actinomycetes in the soil is so important of the soil microflora.

Influência do desponte sôbre a composição do côlmo e do caldo da cana-de-açucar, I. Var. Co 421

I. This paper deals with an experiment carried out to evaluate the effect of the sugar cane upper end on the composition of the sugar cane harvest as a raw material for the sugar industry. The variety studied was Co 421. The authors intend to study other varieties in the future. The data were collected from plant cane, at intervals of two weeks, always from the same field, from a small central area of 3.000 square meters approximately. Sixty (60) stalks were cut in each occasion, randomly chosen from the whole area. They were afterwards separated into three groups of 20 stalks, one for each of the treatments, namely: a) Complete stalks, with no leaves or sheaths. b) Stalks harvested by the technique of REYNOSO, that is, as usually done in practice. c) Stalks with the tops completely cut out, that is, cut by technique of REYNOSO and then with 3 other top internodes eliminated. The treatments caused significant differences on the following technological characteristics: a) Weight b) Cane pol c) Available sucrose (pol) per cent cane d) Cane juice pol e) Saline coefficient of juice. II. Except for weight, all changes were favorable to treatment c, even if with differences relatively slight, in percentage. IIII. Treatment differences for cane fiber, brix, reducing sugars, juice ashes, coefficient of purity and glucose coefficient were not significant. IV. Time of harvest was an important factor affecting the composition of the cane and of the juice. V. On the average the available sucrose of cane for treatments, with an standard error of 0.13%, was: Treatment c 13.05% Treatment b 12.65% Treatment a 12.53% This shows that there is no sound basis for the heavy fines applied by some sugar mills to planters who do not cut low enough the tops of the cane stalks.

Estudos sôbre alimentação mineral do cafeeiro: VIII. Estudo da absorção e da translocação do radiozinco no cafeeiro (Coffea arabica L.)

WATER-CULTURE EXPERIMENTS. Two water-culture experiments were carried out to study the absorption and the translocation of radiozinc in young coffee plants as influenced by two factors, namely, concentration of heavy metals (iron, man ganese, copper and molybdenum) and method of application. Inert zinc was supplied at an uniform rate of 0. 05 p. p. m.; the levels of iron supply were 0, 1.0, and 10.0 p. p.m.; manganese was supplied in three doses 0, 0.5, and 5.0 p. p.m.; copper- 0, 0. 02, and 0. 2 p. p. m.; molybdenum- 0, 0. 01, and 0. 1 p. p. m. When applied to the nutrient solution the activity os the radiozinc (as zinc chloride) was 0. 15 microcuries per plant. In the study of the leaf absorption, Zn65 was supplied at the level of 0. 10 microcuries per plant; in this case the radioative material was brushed either on the lower or on the upper surface or both two pairs of mature leaves. The absorption period was 8 weeks. The radioactivity assay showed the following results: 1 - Among the heavy metals herein investigated the iron concentration did not affect the uptake of the radiozinc; by raising the level of Mn, Cu and Mo ten times, the absorption dropped to 50 per cent and even more when compared with the control plants; when, however, these micronutrients were omitted from the nutrient solution, an increase in the uptake of zinc was registered in the minus Cu treatment only. The effects of high levels of Mn, Cu and Mo probably indicate an interionic competition for a same site on a common binding substance in the cell surface. 2 - The absorption of the radiozinc directly applied to the leaf surface reached levels as high as 8 times that registered when the root uptake took place. Among the three methods of application which have been tried, brushing the lower surface of the leaves proved to be the most effective; this result is easily understood since the stomatal openings of the coffee leaves an preferentially located in the lower surface - in this treatment, about 40 per cent of the activity was absorved and around 12 per cent were translocated either to the old or to the newer organs. Chemical analyses for heavy metals, were carried out only in the plants received Zn65Cl2 in the nutrient solution; the results were as follows; 1 - Control plants had, per 1,000 gm, of dry weight the following amounts in mg.: Zn- 48 in the roots and 29 in the tops; Fe- 165 in the roots and 9 in the tops; Mn- 58 in the roots and 15 in the tops, Cu- 15 in the roots and 1. 2 in the tops; Mo- 2. 8 in the roots and 0. 45 in the tops. 2 - The effect of different levels of micronutrients in the composition of the plants can be summarized as follows: Fe and Zn- when omitted from the nutrient solution, the iron and zinc contents in the roots decreased, no variation being noted in the tops; the higher dosis caused an accumulation in the roots but no apparent effect in the tops; Mn- by omitting this micronutrient a decrease in its content in the roots was noted, where as the concentration in the tops was the same; Mo- no variation in roots and tops contents when molybdenum was omitted; higher dosis of manganese and molybdenum increased the amounts formed both in the roots and in the tops. 3 - The influence of the different concentrations of micronutrients heavy metals on the zinc content of the coffee plants can be described by saying that: Fe and Mo- no marked variation; Mn- no effect when omitted, reduced amount when the high dosis was supplied; Mn- when the plants did not receive manganese the zinc content in roots and tops was the same as in the control plants; a decrease in the zinc content of the total plant occurred when the high dosis was employed; Cu -the situation is similar to that described for manganese. Hence, results showed by the chemical analyses roughly correspond to those of the radioactivity assay; the use of the tracer technique, however, gave best informations along this line. SOIL-POTS EXPERIMENTS. The two types of soils which when selected support the most extensive coffee plantations in the State of São Paulo, Brazil: "arenito de Bauru", a light sandy soil and "terra roxa legitima", a red soil derived from basalt. Besides NPK containing salts, the coffee plants were given two doses of inert zinc (65 and 130 mg ZnCl2 per pot) and radiozinc at a total activity of 10(6) counts/minute. The results of the countings can be summarized as follows: 1 - When plants were grown in "arenito de Bauru" the activity absorbed as per cent of the total activity supplied was not affected by the dosis of inert zinc. The highest value found was around 0. 1 per cent. 2 - For the "terra roxa" plants, the situation is almost the same; there was, however, a slight increase in the absorption of the radiozinc when 130 mgm of ZnClg2 was given: a little above 0. 2 per cent of the activity supplied was absorbed. The results clearly show that the young coffee plants practically did not absorb none of the zinc supplied; two reasons at least could be pointed out to explain such a fact: 1 - Zinc fixation by an exchange with magnesium or by filling holes in the octahedral layer of aluminosilicates, probably kaolinite; 2 - No need for fertilizer zinc in the particular stage of life cycle under which the experiment was set up. The data from chemical analysis are roughly parallel to the above mentioned. When one attempts to compare - by taking data herein reported zinc uptake from nutrient solution, leaf brushing or from fertilizers in the soil, a practical conclusion can be drawn: the control of zinc deficiency in coffee plants should not be done by adding the zinc salts to the soil; in other words: the soil applications used so extensively in other countries seem not to be suitable for our conditions; hence zinc sprays should be used wherever necessary.

Estudos sôbre a Alimentação Mineral do Abacaxí (Ananas sativus) Sch

Pineapple plants when grown in the greenhouse by the sand culture technique in order to study the effects of deficiencies of macronutrients in growth, yield, leaf and fruit composition, the main results were the following. As a result of the several treatments, yield decreased in the order: Complete Minus Mg Minus S Minus Ca Minus K; nitrogen and phosphorus deficiente plants did not bear fruit. Leaf analyses (see Table 5-1) showed that the ommission of given element from the nutrient solution always caused a decrease in its level in the green tissue. As seen in Table 5-2 the lack of macronutrients had certain effects on fruit composition: acidity increased in all cases excet in the minus Mg fruits; ash usually decreased reaching its lowest valued in fruits from the minus K plants; when compared to fruits picked in the "normal" plants, those lacking K showed a marked decrease both in brix and in total sugars as well; sulfur deficiency also brought a net reduction in the sugar content. Table 5-1. Levels of macronutrients found in pinapple leaves. Elements Treatment Percent of dry matter Nitrogen (N) Complete 1.29 Minus N 0.78 Phosphorus (P) Complete 0.12 Minus P .05 Potassium (K) Complete 2.28 Minus K 0.16 Calcium (Ca) Complete 1.19 Minus Ca 1.10 Magnesium (Mg) Complete 0.41 Minus Mg .29 Sulfur (S) Complete 1.00 Minus S .65 Table 5-2. Effects of macronutrients deficiency in yield and fruit characteristics. Treatment Ave. weight of Acidity As per Brix Total sugars fruits (gm) per cent cent per cent Complete 1.031 1.16 0.40 14.7 10.8 Minus N no fruit was produced Minus P no fruit was produced Minus K 246 1.44 0.26 11.9 8.3 Minus Ca 513 1.40 0.35 17.8 14.3 Minus Mg 957 0.97 0.38 15.4 13.0 Minus S 576 1.42 0.46 17.1 6.5

Estudos sôbre a alimentação mineral do cafeeiro, X: extração de macronutrientes na colheita pelas variedades "Mundo Novo", "Caturra" e "Bourbon Amarelo"

This paper deals with the determination of the content of macronutrients in pulp and beans of three coffee varieties, namely 'Mundo Novo', 'Caturra Amarelo' and 'Bourbon Amarelo'. Samples were collected in plantations located in the three types of soils herein most of S. Paulo, Brazil, coffee is grown, that is, "terra roxa legítima" (Ribeirão Preto), "massapé-salmourão" (Mocóca), and "arenito de Bauru" (Pindorama). The following main conclusions were drawn after statistical analysis of data obtained hereby. There is no statistical difference among the three varieties . Average contents of macronutrients, as per cent of the dry matter, are the following: N P K Ca Mg S bean 1,71 0,10 1,53 0,27 0,15 0,12 pulps 1.78 0,14 3,75 0,41 0,13 0,15 Samples collected in Mocóca ("massapé-salmourão") had lower N and K contents, probably due to lack of availability of these elements in the soil, as suggested by its analysis. Results obtained in this work are in good agreement with data described elsewhere. Out of the total of elements contained in the whole fruit the following proportions are exported as clean coffee: N - 2/3, P and K - 1/2, Ca, Mg and S - 1/3. It is clear therefore that a substantial amount of elements absorbed from the soil remains in the pulp or in the dry hulls which result from processing. From this fact raises the interest of using these residues as fertilizer in the coffee plantations.

Influência do desponte sôbre a composição do colmo e do caldo da cana-de-açúcar: III. Var. CB 41-76

I. This paper deals with an experiment carried out to evaluate the effect of sugar cane upper end on the composition of the stalks and juice of sugar cane harvest as a raw material for the sugar industry. The variety studied was CB 41-76. The data were collected from plant cane at intervals of a two weeks, always from the same field, from a small central area of 3.000 square meters approximately, 60 stalks were cut in each occasion, randomly chosen from the whole area. They were afterwards separated into three groups of 20 stalks one for each of the treatments, namely: a) Complete stalk, with no leaves or sheaths. b) Stalks harvested by the technique of REYNOSO, that is, as usually done in practice. c) Stalks with the tops completely cut out, that is, cut by the techinique of REYNOSO and then with 3 other top internodes eliminated. The treatments caused significant differences on the weight of cane and coefficient of purity of juice, but the percentual differences between the average treatments a and c is 13% and 2%, respectively. II. Treatment differences for cane pol, cane fibre, brix, juice pol, reducing sugars, juice ashes, glucose coefficient, saline coefficient and available sucrose (pol) per cent were not significant. III. Time of harvest was an important factor affecting the composition of the cane and the juice. This paper shows that there is no sound basis for the heavy fines applied some sugar mills to planters who do not cut low enough the tops of the cane stalks.

Behavior at a nest of Amazilia lactea (Aves, Trochilidae)

The hummingbird Amazilia lactea (Lesson, 1832) built a nest in São Paulo, Brazil, in the spring (Oct) and added lichens during incubation. The female incubated over 70 per cent of the day, 1-56 min per visit, and brooded two small young somewhat less; brooding stopped by about 10 days of age, as did night brooding. Lack of night brooding for large young hummingbirds may reflect lack of space in a small nest. Young stayed in the nest 19 days. Feedings were widely spaced, and presence of possible predators caused alarm.

Dissertationes academicae Vpsaliae habitae svb praesidio Carol. Petr. Thvnberg ...

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Dissertationes academicae Vpsaliae habitae svb praesidio Carol. Petr. Thvnberg ...

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Sobre alguns casos de carcinoma primitivo do pulmão, do bronchio e da trachéa

1-Out of 1531 autopsies at Rio de Janeiro, Brazil, recorded in the Section of Pathology of «Instituto OSWALDO CRUZ», we found three cases of primary carcinoma of the lung, which gives the incidence of 0,195 per cent. They were not associated with tuberculos's and anthracos's was not marked. 2-The gross and microscopical features indicate that they are in reality primary cancers of the larger bronchi arising probably, from the bronchial lining epithelium. There is a striking similarity both macro and microscopical in Cases I and II, where the structure is that of cylindrical cell alveolar carcinoma. Mucus formation was never a prominent feature in these tumors. As in HENRICI's case they apparently invade the lung along the alveolar wall in more or less considerable extension retaining the alveolar septa as stroma. In case III the structure is that of squamous cell carcinoma, and the tumor mass, usually ulcerating, lies in the immediate vicinity of the bronchi, the extension to the lung tissue being not widespread. 3-Cases IV and V are tumors of the lower and median portion of the trachéa, squamous cell carcinoma in structure, which form marked contrast with the tumors originating in the larger bronchi: the tumor process never invades the lung parenchyma by continuous extension. In Case V extension to the mediastinal lymph-nodes is observed, and a tumor of the size of an orange is found in the anterior mediastinum: that tumor mass and the upper lobe of the right lung were only adherent by fibrous tissue of inflammatory origin and the tumor did not extend into the lung tissue.

Glutathion no sangue humano normal

1.) - Woodward-Fry's and Okuda-Hess technics were employed in the determination of blood glutathione in normal healthy adults of both sexes. 2.) - It was found more accurately results with the technic of Woodward and Fry than of any others for the dosage of G. S. H. of blood. 3.) - When the process of Okuda-Hess is modified by the use of an intern indicator (starch) the readings of the end-point are much more easy and therefore the results more exacts. 4.) - The averages of the data obtained for normal blood by the technic of Woodward and Fry were for men per hundred cubic cent. 27 mgrs (G.S.H); 6.6 mgrs. (G.S.S) and 33.6 mges. (G. T) and for women: 28.4 mgrs. (G.S.H), 7.8 mgrs. (G.S.S) and 36,2 mgrs. (G.T). 5.) - Autoxidation in the blood filtrate is only apreciated after 24 hs. In the first eight hours autoxidation is never observed. 6.) - The increase of glutathione in hyperglobulia is a function of the amount of red corpuscles. In acrocyanosis arterial blood is richest in these component than venous blood and this fact is in accordance with the observation of Blanchetière, Mélon and Binet for the experimental asphyxia of dogs.

Estudos sôbre a excitação química da córtex cerebral (Ação da acetilcolina)

The author has studied the influence of acetylcholine solutions directly applied on the motor cortex of dogs, cats monkeys and rabbits. For this purpose small squares of filter paper were soaked in the acetylcholine solution and soon afterwards laid on the motor cortex. Solutions varying from 0,2 to 10 per cent have been experimented. It has been shown that local application of the solutions on the motor points, previously localized by induction coil, produced motor reactions. It has been found, in the dogs that 10 per cent acetylcholine solutions cause localized muscular twitchings (clonus) in almost all the animals experimented. Generalised epileptiform convulsions were obtained in44,4% of the dogs. Convulsions were also obtained by employing 1 per cent solution of acetylcholine. Definite response has been obtained with 0,2 per cent solution. Failure of motor action, pointed out by other authors, has been related to the use of anesthetics. Convulsions were easily produced by rapid light mechanical stimulations of the skin covering the muscles in conection with the excited motor point, and the application on the motor point of acetylcholine. The results on monkeys can be summarized as follows. Two species of monkeys were experimented: Cebus capucinus and Macaca mulata. In the monkeys C. capucinus generalised convulsive reactions were induced with actylcholine solutions in a concentration as low as 0,5 per cent. Motor reaction or convulsive seizeres were obtained in seven of the eight monkeys used. Three monkeys M. mulata were stimulated with 10 per cent acetylcholine solution but only localized muscular contraction hae been observed. Similar results has been obtained on the motor cortex of cats and rabbits. One of the three cats employed has shown epileptiform convulsions and the remaining only localized muscular contractions. In the rabbits muscular twitchings have been also induced. The sensitizing power of eserine on the action of acetylcholine has been also searched. The results indicate that a previous application of eserine solution on the motor center, potentiates the action of acetylcholine. The intensity of the muscular twitchings is greater than the obtained before the application of the eserine solution. Generalised epileptiform convulsions sometimes appeared following the use of lower concentrations of acetylcholine than those previously employed. Experiments have been carried out by injecting eserine and prostigmine by parenteral route. A dosis dufficient for induce small muscular tremors did not enhance obviously the motor effects produced by the application of the acetylcholine solutions on the motor cortex. From seven dogs experimented, all previously tested for convulsive seiruzes by application of 1 and 10 per cent acetylcholine solution with negative results, only one has shown epileptiform convulsions after the injection of prostigmine. Morphine has also been tested as facilitating substance for convulsions induced by acetylcholine. Six from the nine dogs submitted to the experiments, developed epileptiform seizures after injection of morphine and stimulation of the motor cortex with acetylcholine. (Table IV). In another series of experiments atropine and nicotine have been studied as for to their action on the motor effects of acetylcholine. Nicotine has a strong convulsant action, even when employed in very high concentration. Since a depressant effect has not appeared even by the applications of high concentrations of nicotine in the motor corteõ of dogs, unlike the classical observations for the autonomus nervous system, it was not possible to verify the action of acetylcholine on a motor center paralised by nicotine. It is important to not that the motor phenomena observed after the first aplication of acetylcholine, can desappear by the renewal of the pieces of filter paper soaked in the acetylcholine solution. Atropine, either applied on the motor point in low concentration, or injected in sufficient amount for inhibiting the “muscarinic effects” of acetylcholine on the autonomous nervous system, did not prevent the motor reactions of acetylcholine on the cerebral cortex.