Development of Gene Expression Markers of Acute Heat-Light Stress in Reef-Building Corals of the Genus Porites

Coral reefs are declining worldwide due to increased incidence of climate-induced coral bleaching, which will have widespread biodiversity and economic impacts. A simple method to measure the sub-bleaching level of heat-light stress experienced by corals would greatly inform reef management practices by making it possible to assess the distribution of bleaching risks among individual reef sites. Gene expression analysis based on quantitative PCR (qPCR) can be used as a diagnostic tool to determine coral condition in situ. We evaluated the expression of 13 candidate genes during heat-light stress in a common Caribbean coral Porites astreoides, and observed strong and consistent changes in gene expression in two independent experiments. Furthermore, we found that the apparent return to baseline expression levels during a recovery phase was rapid, despite visible signs of colony bleaching. We show that the response to acute heat-light stress in P. astreoides can be monitored by measuring the difference in expression of only two genes: Hsp16 and actin. We demonstrate that this assay discriminates between corals sampled from two field sites experiencing different temperatures. We also show that the assay is applicable to an Indo-Pacific congener, P. lobata, and therefore could potentially be used to diagnose acute heat-light stress on coral reefs worldwide.

The Enchanter's Spell: J.R.R. Tolkien's Mythopoetic Response to Modernism

J.R.R. Tolkien was not only an author of fantasy but also a philologist who theorized about myth. Theorists have employed various methods of analyzing myth, and this thesis integrates several analyses, including Tolkien’s. I address the roles of doctrine, ritual, cross-cultural patterns, mythic expressions in literature, the literary effect of myth, evolution of language and consciousness, and individual invention over inheritance and diffusion. Beyond Tolkien’s English and Catholic background, I argue for eclectic influence on Tolkien, including resonance with Buddhism. Tolkien views mythopoeia, literary mythmaking, in terms of sub-creation, human invention in the image of God as creator. Key mythopoetic tools include eucatastrophe, the happy ending’s sudden turn to poignant joy, and enchantment, the realization of imagined wonder, which is epitomized by the character of Tom Bombadil and contrasted with modernist techno-magic seeking to alter and dominate the world. I conclude by interpreting Tolkien’s mythmaking as a form of mysticism.

Questionamentos acerca de valores axiológicos no turismo: uma abordagem fenomenológica hermenêutica

The study presents the possibility of interpretation of axiological values of tourism as a practice conceived on a human being or Dasein as being-in-the-world of tourism. The value, as an object of Axiology, was considered the predictor of the human being conduct in the phenomenon reflecting this same value in tourism. The aim was to comprehend and interpret through the way of being of Dasein in tourism, which axiological values are chosen to the practice of tourism and the intentional feelings directed to these values. A phenomenological hermeneutics research with exploratory characteristics was accomplished in order to survey the values. Ten episodic interviews were conducted from the hermeneutic situation - constituted by fore-having, fore-sight and fore-conception of each Dasein interviewed, by adopting a sympathetic conduct and sympathy of Max Scheler and the use of emotional intuition to capture the intentional feelings, interpreted afterwards by the analysis of a Martin Heidegger's phenomenology in Being and Time. The results showed that, even without categorisation, the totality of the living experiences, the way of being of positive values outnumber the negatives ones in the existence of each Dasein, leading them to the Learning, which are comprising: experiences to provide self-knowledge, historical-cultural values, and memory as part of the learning experiences, hospitality as a way of openness and socio-cultural exchange, solidarity and peace. Intentional feelings directed at the values for the choice for practicing tourism were: love, happiness, pleasure, respect and trust. Four evidences were found concerning the use of sentimental perspective and intentional feeling of Scheler and regarding the logic of the heart of Pascal used by this author. The sociocultural interrelationships and exchanges form the basis for developing tourism as phenomenon. Therefore the character being-with or Mitsein is prevalent in tourism activities. Despite the learning was the purpose of the experiences, the ultimate goal was the improvement and personal enrichment of Dasein´s humanity development. The study also showed the hermeneutic phenomenological seeing opens the access of the living experiences of values, without making arbitrarily judgment and achieve "to the thing themselves", which, by the overlapping of categories, dispositions and intentional feelings, form the evaluative experiences and are possible to access through the fundamental ontology of Heidegger. The study contributes to broaden the vision concerning to the totality of tourism and the practitioners Dasein of it. As possibilities for deepening studies, was pointed out: the total person of Scheler; the care or Sorge as a form of love in Heidegger; happiness and pleasure in the practice of tourism and human flourishing or eudaimonia.

Relative contents of foraminifera species in bottom sediments of northern and southern seas

Correlation of paleoceanographic events in several key regions of the World Ocean: North Atlantic, Antarctic, West Arctic Seas, North Pacific and tropical Indo-Pacific has been carried out for the last 135 ka based on micropaleontological, stable isotope, geochronological (AMS-14C) and other data. It has been shown that the global thermohaline circulation controls remote climatic teleconnections on millennial-scale and partly on centennial-scale, while short-term climate changes are mainly transferred by the atmosphere. The basic information is given about the recent thermohaline circulation and stages of its development during Neogene.

Non-indigenous species in the North and Baltic Seas and the Great Lakes-St. Lawrence River region

List of non-indigenous species (NIS) established in the Great Lakes-St. Lawrence River region and the North and Baltic Seas region, their geographic origin, and taxonomic assignment. Asterisks mark the NIS that occur in both the North and Baltic Seas and the Great Lakes-St. Lawrence River regions. GL, SL, NW, NE, SW and SE denote the Great Lakes, St. Lawrence River, north-west, north-east, south-west, and south-east, respectively. Eurasia represents inland freshwaters except Yangtze River, Indo-Pacific represents Indian Ocean and the archipelago of Indonesia, Malaysia, and Pilipinas, North America (N America) represents inland freshwaters except the Laurentian Great Lakes, St. Lawrence and Mississippi Rivers, while Australia, New Zealand, Africa and South America (S America) cover all inland freshwaters in these areas.

(Table 1) Sr, Nd and Os isotopic composition from marine sediments of the Bengal Fan

Sr, Nd, and Os isotopic data are presented for sediments from diverse locations in the Bay of Bengal. These data allow the samples to be divided into three groups, related to their sedimentary contexts. The first group, mainly composed of sediments from the shelf off Bangladesh and the currently active fan, has Sr and Nd characteristics consistent with a dominantly Himalayan source. Their 187Os/188Os ratios (~1.2-1.5) show that the average detrital material delivered by the Ganga-Brahmaputra (G-B) river system is not unusually radiogenic. A large difference in 187Os/188Os ratio exists between these Bengal Fan sediments and Ganga bedloads (187Os/188Os ~2.5, Pierson-Wickmann et al. (2000, doi:10.1016/S0012-821X(00)00003-0)). This difference mainly reflects addition of a less radiogenic Brahmaputra component, though mineralogical sorting and loss of radiogenic Os during transport may also play some role. The second sample group contains sediments from elsewhere in the Bay, particularly those located on the continental slope. They display Os isotopic compositions (0.99-1.11) similar to that of present seawater and higher Os and Re concentrations. These characteristics suggest the presence of a large hydrogenous contribution, consistent with the lower sedimentation rate of these samples. Sr and Nd ratios indicate that a significant fraction of these sediments is derived from erosion of non-Himalayan sources, such as the Indo-Burman range. These observations could be explained by the deflection of sediments from the G-B river system by westward currents in the head of the Bay. The third group contains only one sample, but shows that in addition to a Himalayan source, sediment discharge from Sri Lanka may influence the detrital component in the distal part of the fan. The similarity between the isotopic compositions of the group I R/V Sonne samples and those of Ocean Drilling Program Leg 116 (France-Lanord et al., 1993; Reisberg et al., 1997, doi:10.1016/S0012-821X(00)00003-0) suggests that the material eroding in the Himalayas has been roughly constant since the Miocene. The high Os isotopic ratios of leachates of both Sonne group I and Miocene Leg 116 sediments imply that much of the leachable highly radiogenic Os component was conserved during transport through the estuary or interaction with seawater. In constrast, samples with lower, but still relatively high, sedimentation rates (Sonne groups II and III and Pliocene Leg 116) seem to have significantly adsorbed or exchanged Os and Re with seawater. This suggests that in some cases the Os isotopic ratios of leachates of detrital sediments can be used to constrain the ancient marine Os record, or conversely, to date unfossiliferous sediments.

Stable isotope ratios of a oyster Hyotissa hyotis from the northern Red Sea

This study explores the giant oyster Hyotissa hyotis as a novel environmental archive in tropical reef environments of the Indo-Pacific. The species is a typical accessory component in coral reefs, can reach sizes of tens of centimetres, and dates back to the Late Pleistocene. Here, a 70.2-mm-long oxygen and carbon isotope transect through the shell of a specimen collected at Safaga Bay, northern Red Sea, in May 1996, is presented. The transect runs perpendicularly to the foliate and vesicular layers of the inner ostracum near the ligament area of the oyster. The measured d18O and d13C records show sinusoidal fluctuations, which are independent of shell microstructure. The d13C fluctuations exhibit the same wavelength as the d18O fluctuations but are phase shifted. The d18O record reflects the sea surface temperature variations from 1957 until 1996, possibly additionally influenced by the local evaporation. Due to locally enhanced evaporation in the semi-enclosed Safaga Bay, the d18Oseawater value is estimated at 2.17 per mil, i.e., 0.3-0.8 per mil higher than published open surface water d18O values (1.36-1.85 per mil) from the region. The mean water temperature deviates by only 0.4°C from the expected value, and the minimum and maximum values are 0.5°C lower and 2.9°C higher, respectively. When comparing the mean monthly values, however, the sea surface temperature discrepancy between reconstructed and global grid datasets is always <1.0°C. The d13C signal is weakly negatively correlated with regional chlorophyll a concentration and with the sunshine duration, which may reflect changes in the bivalve's respiration. The study emphasises the palaeogeographic context in isotope studies based on fossils, because coastal embayments might not reflect open-water oceanographic conditions.

Prática pedagógica em educação de infância : consciência fonológica e desenvolvimento de competências orais e escritas

Relatório de estágio para a obtenção do grau de mestre em Educação pré-escolar e 1º Ciclo do ensino básico

A noção de sistema na fundação da linguística moderna

This work is intended to investigate the saussurian notion of system. Such a notion is fundamental to Ferdinand de Saussure's theorization, since it composes the definition of "langue", as he thought it. This definition was crucial to the delimitation of linguistics' specific object of study, which granted its place among modern sciences. However, the notion of system was not created by Saussure. Not only in Linguistics, but also in other areas, this notion appeared in very ancient studies, mingling with the establishment of man in society and the development of their economic and organizational activities. Specifically, in the context of language studies, the system consists in a notion that composed the work of the first grammarians in the West, in ancient Greece. Moreover, this notion was also used afterwards, in the synonymy studies and in the comparative analysis of languages, developed by scholars of the nineteenth century. Nevertheless, although Saussure had had his formation in Leipzig and Berlim, amid comparatists studies, his notion of system is an innovation, while is also continuing. In light of this, we aim to highlight the aspects of the saussurian notion of system that allow the establishment of a relationship of continuity and rupture with other conceptions of system. For that, we will investigate four Saussure authored documents: the « Cours de linguistique générale », the « Mémoire sur le système primitif des voyelles dans les langues indo-européennes », and the two sets of manuscripts « De l'essence double du langage » and « Notes pour le cours III ».

Caminhada de aprendizagens em Educação de Infância e em 1.º Ciclo do Ensino Básico. Interação entre pares incluindo uma criança com Necessidades Educativas Especiais.

Este relatório foi efetuado no âmbito do Mestrado em Educação Pré-Escolar e Ensino do 1.º Ciclo do Ensino Básico (CEB), e centra-se em duas dimensões diferentes, a reflexiva e a investigativa. Na dimensão reflexiva encontram-se reflexões críticas e fundamentadas que demonstram as minhas vivências e aprendizagens mais significativas ao longo dos distintos contextos de intervenção, particularmente no contexto de Educação de Infância e de 1.º CEB. A dimensão investigativa apresenta um estudo realizado com quatro crianças de um jardim-de-infância. Trata-se de um estudo caso que se situa num paradigma interpretativo. O principal objetivo foi identificar e conhecer o tipo de interações estabelecidas entre uma criança com necessidades educativas especiais e os seus pares nos momentos de brincadeira livre, assim como as conceções que as crianças e as famílias tinham acerca dessas interações. Indo ao encontro dos objetivos definidos, os dados foram recolhidos através de observações, entrevistas e inquéritos por questionário. A análise dos dados permite verificar, que as crianças interagiram com a criança com necessidades educativas especiais de diferentes formas - com e sem mediação de objetos. Destaca-se que o objeto atua como mediador da interação social, e portanto, permite a aproximação das crianças. Sobressai a conceção das crianças acerca da interação com mediação de objetos que ocorre entre pares incluindo uma criança com necessidades educativas especiais, ao contrário da conceção das famílias que ressaltam as interações sem a mediação de objetos.

Dahlak coral d18O record (DGII) from the southern Red Sea

Coral palaeoclimatic studies are under way at many sites throughout the wet tropics. However, arid environments have received less attention. Here we report a high-resolution, 63 yr record of coral d18O and d13C extracted from a Porites colony from the Dahlak Archipelago, off the Eritrean coast, in the southern Red Sea. The annual cycles of the coral d18O and d13C are inversely related while their inter-annual variations show a strong positive correlation, with similar inter-decadal trends. Inter-annual variations in coral d18O show a relatively weak correlation with the southern Red Sea SST, but are strongly correlated with the Indian Ocean SST, especially on the decadal time-scale. The range of the inter-annual variations in the coral d18O is high compared to changes in local SST, due to the amplifying effect of simultaneous changes in water isotopic composition. Due to this amplification of the climate signal the coral provides a better indication of regional oceangraphic behaviour than the local SST record. The norrtheast monsoon signal in the coral d18O dominates the mean annual signal and shows the best correlation with the instrumental data sets. It appears that variations in the coral d18O are controlled mainly by variations in the intensity of surface water influx from the Indian Ocean to the Red Sea during the winter northeast monsoon. Of particular significance is that the decadal time-scale variations in the coral skeletal d18O are closely correlated with both the Indian Ocean SST and with variations in the Pacific-based Southern Oscillation index. That is, isotopically light coral skeleton, indicating strong NE monsoon Red Sea inflow, correlates with periods of high Indian Ocean SST and with predominantly negative (El Nino) phases of the Southern Oscillation. The simultaneous nature of inter-decadal changes in Asian monsoon and ENSO behaviour suggest pan-Indo-Pacific tropical climate reorganisation and evolution.

Benthic foraminiferal abundances from eight sites of the Northwest Australian shelf

Foraminiferal analysis of Miocene to recent strata of the Northwest Shelf of Australia is used to chart West Pacific Warm Pool (WPWP) influence. The assemblage is typified by "larger" foraminifera with ingressions of the Indo-Pacific "smaller" taxa Asterorotalia and Pseudorotalia at around 4 Ma and from 1.6 to 0.8 Ma. A review of recent and fossil biogeography of these taxa suggests their stratigraphic distribution can be used to document WPWP evolution. From 10 to 4.4 Ma a lack of biogeographic connectivity between the Pacific and Indian Ocean suggests Indonesian Throughflow (ITF) restriction. During this period, the collision of Australia and Asia trapped warmer waters in the Pacific, creating a central WPWP biogeographic province from the equator to 26°N. By 3 Ma Indo-Pacific species migrated to Japan with the initiation of the "modern" Kuroshio Current coinciding with the intensification of the North Pacific Gyre and Northern Hemisphere ice sheet expansion. Indo-Pacific taxa migrated to the northwest Australia from 4.4 to 4 Ma possibly because of limited ITF. The absence of Indo-Pacific taxa in northwest Australia indicates possible ITF restriction from 4 to 1.6 Ma. Full northwest Australian biogeographic connectivity with the WPWP from 1.6 to 0.8 Ma suggests an unrestricted stronger ITF (compared to today) and the initiation of the modern Leeuwin Current. The extinction of some Indo-Pacific species in northwest Australia after 0.8 Ma may be related to the effects of large glacial/interglacial oscillations and uplift of the Indonesian Archipelago causing Indonesian seaway restriction.

Physiological and ecological variables measured at the high and low pCO2 reef sections

Experiments have shown that ocean acidification due to rising atmospheric carbon dioxide concentrations has deleterious effects on the performance of many marine organisms. However, few empirical or modelling studies have addressed the long-term consequences of ocean acidification for marine ecosystems. Here we show that as pH declines from 8.1 to 7.8 (the change expected if atmospheric carbon dioxide concentrations increase from 390 to 750 ppm, consistent with some scenarios for the end of this century) some organisms benefit, but many more lose out. We investigated coral reefs, seagrasses and sediments that are acclimatized to low pH at three cool and shallow volcanic carbon dioxide seeps in Papua New Guinea. At reduced pH, we observed reductions in coral diversity, recruitment and abundances of structurally complex framework builders, and shifts in competitive interactions between taxa. However, coral cover remained constant between pH 8.1 and ~7.8, because massive Porites corals established dominance over structural corals, despite low rates of calcification. Reef development ceased below pH 7.7. Our empirical data from this unique field setting confirm model predictions that ocean acidification, together with temperature stress, will probably lead to severely reduced diversity, structural complexity and resilience of Indo-Pacific coral reefs within this century.

Stable isotope ratios of benthic and planktonic foraminifera from Miocene to Pliocene sediments of DSDP Hole 7-62A on the Eauripik Rise, Pacific Ocean (Table 1)

Oxygen and carbon isotope analyses show that the biserial forarniniferal genus Streptochilus, which was originally described from pelagic sediments on the Eauripik Rise and Ontong Java Plateau, lived deep in the upper water column within the oxygen minimum layer. The species of Streptochilus average from 4 to 19% of the foraminiferal assemblages in which benthic forms compose less than 1 or 2%. Specimens of Streptochilus are selectively dissolved when in contact with the bottom water mass. Their rapid evolutionary turnover of less than a few million years and their wide areal distribution in the equatorial Indo-Pacific are indicative of planktonic foraminifera. Aside from usefulness of the species of Streptochilus as stratigraphic indices, these Neogene biserial planktonic foraminifera are potential indices of paleoceanographic stratification.