Cloning and characterization of a blood coagulation factor IX-binding protein from the venom of Trimeresurus stejnegeri

A blood coagulation factor IX-binding protein (TSV-FIX-BP) was isolated from the snake venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-FIX-BP showed a single band with an apparent molecular weight of 23,000 under non-reducing conditions. and two distinct bands with apparent molecular weights of 14,800 and 14,000 under reducing conditions. cDNA clones containing the coding sequences of TSV-FIX-BP were isolated and sequenced to determine the structure of the precusors of TSV-FIX-BP subunits. The deduced amino acid sequences of two subunits of TSV-FIX-BP were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. TSV-FIX-BP was a nonenzymatic C-type lectin-like anti-coagulant. The anti-coagulant activity of TSV-FIX-BP was mainly caused by its dose dependent interaction with blood coagulation factor IX but not with blood coagulation factor X. (C) 2003 Elsevier Science Ltd. All rights reserved.

Wavelength-tuneable liquid crystal lasers from the visible to the near-infrared

The study of band-edge lasing from dye-doped chiral nematic liquid crystals has thus far been largely restricted to visible wavelengths. In this paper, a wide range of commercially available laser dyes are examined for their suitability as infrared emitters within a chiral nematic host. Problems such as poor solubility and reduced quantum efficiencies are overcome, and successful band-edge lasing is demonstrated within the range of 735-850 nm, using the dyes LD800, HITC-P and DOTC-P. This paper also reports on progress towards widely tuneable liquid crystal lasers, capable of emission in the region 460- 850 nm. Key to this is the use of common pump source, capable of simultaneously exciting all of the dyes (both infrared and visible) that are present within the system. Towards this aim, we successfully demonstrate near-infrared lasing (800 nm) facilitated by Förster energy transfer between the visible dye DCM, and the infra-red dye LD800, enabling pump wavelengths anywhere between 420 and 532 nm to be used. These results demonstrate that small and low-cost tuneable visible to near-infrared laser sources are achievable, using a single common pump source. Such devices are envisaged to have wide-ranging applications including medical imaging (including optical coherence tomography), point-of-care optical medical diagnostics (such as flow cytometry), telecommunications, and optical signatures for security coatings. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

Structural features of geostrophic circulation in open region of the South China Sea

On the basis of observation data of water temperature and salinity the mean seasonal geostrophic circulation in open region of the South China Sea (SCS) was computed by the dynamic method relative to the 800 decibar reference surface. The results of computation let go to following notices: In both main monsoons (winter and summer) there are two main geostrophic eddies: the anticlockwise eddy in the northern and northwestern part, and the clockwise eddy in the southern part of the SCS with corresponding divergent and convergent zones. The main frontal zones go along the middle latitudes of the sea from the southern continental shelf of Vietnam to the area west of Luzon Island. The strength and stability of the current in winter are higher than in summer. The Kuroshio has an enough strong branch intruding into the SCS through Bashi Strait in winter creating in the sea the water structure similar to that of the Northwest Pacific subtropical and tropical regions. In summer the Kuroshio water can intrude directly only into the area southwest of Taiwan.

Anti Human Immunodeficiency Virus-1 (HIV-1) Agents 3. Synthesis and in Vitro Anti-HIV-1 Activity of Some N-Arylsulfonylindoles

In order to find compounds with superior anti human immunodeficiency virus type 1 (HIV-1) activity, twelve simple N-arylsulfonylindoles (3a-1) were synthesized and preliminarily evaluated as HIV-1 inhibitors in vitro for the first time. Several compounds

Assessment of impact of ten days fishing ban in the major spawning grounds of hilsa (Tenualosa ilisha, Fisher and Bianchi, 1984)

A study was undertaken to find out the impact of ten days fishing ban in the major spawning grounds of hilsa during October to December. The study revealed a positive impact of fishing ban during spawning season on reproduction success of hilsa. Proportionate distribution of male and female hilsa also showed a significant level of distribution in and around the spawning grounds where the ratio was 35:65. Percent of oozing hilsa during fishing ban period in the spawning grounds was observed 1.61%. About 5% spent hilsa was observed in the fish landing centers and was compared with the data of Global Environment Facility (GEF) and Bangladesh Fisheries Research Institute (BFRI) studies and was found about 2.80-3.57 times higher than that of the findings of 2002 and 2003. Estimated egg production value showed about 46,800 Kg of eggs could have been produced that indicating a positive impact of 10 days fishing ban in spawning season. Abundance of higher percentages of gravid hilsa was found which were not available in the same quantity and condition in the non-fishing ban period. During the present investigation, fairly higher amount of spent hilsa and juveniles were also observed in the spawning grounds. Along with the jatka fry, spawn and fries of other fishes were also found in higher quantity than the previous years and thus it is assumed that fishing ban also might have positive impact on the successful breeding of other fishes. Overall, the fishing ban was found effective for successful breeding of hilsa.

Long-term exposure to extremely low-frequency magnetic fields impairs spatial recognition memory in mice

1. In the present study, we investigated the short- and long-term effects of extremely low-frequency (ELF) magnetic fields on spatial recognition memory in mice by using a two-trial recognition Y-maze that is based on the innate tendency of rodents to exp

Water budgeting studies on the hatchery and nursery rearing practices for the common carp, Cyprinus carpio (Linnaeus, 1758)

Aquaculture systems are an integral element of rural development and therefore should be environment friendly as well as socially and economically designed. From the economic standpoint, one of the major constraints for the development of sustainable aquaculture includes externalities generated by competition in access to a limited resource. This study was conducted as an investigation into the water requirement for the hatchery and nursery production phases of common carp, Cyprinus carpio (Linnaeus, 1758) at the Maharashtra State Fish Seed Farm at Khopoli in Raigad Dist. of Maharashtra during the winter months from November to February. The water budgeting study involves the quantification of water used in every stage of production in hatchery and nursery systems and aimed at becoming a foundation for the minimization of water during production without affecting the yield; thereby conserving water and upholding the theme of sustainable aquaculture. The total water used in a single operation cycle was estimated to be 11,25,040 L [sic]. Out of the total water consumed, 4.74% water was used in the pre-operational management steps, 4.48% was consumed during breeding, 62.72% was consumed in the hatching phase, 21.50% was used for hatchery rearing and 6.56% was consumed during conditioning. In the nursery ponds, the water gain was primarily the regulated inflow coming through the irrigation channel. The total quantum of water used in the nursery rearing was 31,60,800 L [sic]. The initial filling and regulated inflow formed 42.60% and 57.40% respectively of water gain, while evaporation, seepage and discharge contributed 20.71%, 36.46% and 42.82% respectively to the water loss. The total water expended for the entire operation was 1,21,61,120 L [sic]. Water expense occurred to produce a single spawn in the hatchery system was calculated and found to be 0.56 L while the water expended to produce one fry was calculated as 4.86 L. The study fulfills the hydrological equation described by Winter (1981) and Boyd (1985). It also validates the water budget simulation model that can be used for forecasting water requirements for aquaculture ponds (Nath and Bolte, 1998).

绿孔雀在中国的分布现状调查

绿孔雀在中国现仅见于云南西部、中部和南部, 通过1991年至1993年的调查, 绿孔雀现存数量较多的地区有云南省瑞丽县、陇川县、昌宁县、永德县、新平县、普洱县、墨江县、景东县、楚雄市、双柏县、南华县。过去有分布记 录现已绝迹或濒临绝迹的地区有盈江县、泸水县、腾冲县、蒙自县、河口县。永仁县的中和、直左为新近发现的分布点, 据当地群众反映, 在维西县的叶枝、德钦县的拖顶和奔子栏也发现绿孔雀。由于栖息地生境的破坏, 导致绿孔雀现存种群形成小家族群点状隔离分布。据估计, 中国现存野生种群数量约为800-1100只。图1表1参9

Evolution of the tilapia fishery with specific reference to the Nile tilapia (Oreochromis niloticus Linne)

As a fishery, the immensely large (c. 68,800 km2 ) Lake Victoria is a unique ecosystem which together with a riverine connection to the Lake Kyoga basin share a common endemic "Victorian" fish fauna (Greenwood 1966). Until the 1950s, the single socio economically most important species of fish in these two lakes was the native Oreochromis esculentus Graham (Graham 1929) even though the lake also contained a second native tilapiine, 0reochromis variabilis , and over 300 other fish species (Beauchamp, 1956).

The potential for clupeid fishery in Lake Kainji, Nigeria

The potential for a clupcid fishery in Lake Kainji has been examined using both experimental fishing samples and the results obtained from those of the commercial fishermen. A total annual production of 1,400 tons has been estimated. In money terms, this amounls to N198.800 (£99,400) per annum. This production has been sustained by the abundant zooplankton food available the year round on the lake. Recommendations for the effective methods of cropping clupeids were made, bearing in mind the need to maintain the fishery on a sustained yield basis. The place of c1upeids in the ecology of the lake was examined and on the basis of the evidence available it was recommended that Pellonula afzeluisi could be exploited on a large commercial scale without any adverse effects on the fishery in the lake.

Enhancement of magnetostriction in internally-biased Terfenol-D 2-2 composites

A key issue in the fabrication of Terfenol-D 2-2 composites with internal magnetic field biasing is the selection of appropriate constituent materials to obtain high magnetostriction while keeping optimum magnetomechanical properties. The fabrication process is costly and time consuming and, therefore, numerical methods to predict their properties are useful. In this paper, finite element analysis (FEA) of the magnetostriction of such composites has been carried out using the commercial package ABAQUS. It has been shown that composites fabricated using Nd2Fe14B for the permanent magnetic material layers possess the highest internal fields within the Terfenol-D layers, although the overall strain of these composites is limited to approximately 800 × 10-6 due to the high elastic modulus of Nd2Fe14B. Simulations showed that the strain can be enhanced by choosing a different material with a lower elastic modulus for the permanent magnetic layer even though the internal field is lower. The simulations showed that the strain can increase by 12% if the Nd 2Fe14B layer is substituted by SmCo5; by 23% if it is substituted by Sm2Co17; and by 35% if it is substituted by Alnico. © 2008 IEEE.

Wavelength-tuneable liquid crystal lasers from the visible to the near-infrared

The study of band-edge lasing from dye-doped chiral nematic liquid crystals has thus far been largely restricted to visible wavelengths. In this paper, a wide range of commercially available laser dyes are examined for their suitability as infrared emitters within a chiral nematic host. Problems such as poor solubility and reduced quantum efficiencies are overcome, and successful band-edge lasing is demonstrated within the range of 735-850 nm, using the dyes LD800, HITC-P and DOTC-P. This paper also reports on progress towards widely tuneable liquid crystal lasers, capable of emission in the region 460- 850 nm. Key to this is the use of common pump source, capable of simultaneously exciting all of the dyes (both infrared and visible) that are present within the system. Towards this aim, we successfully demonstrate near-infrared lasing (800 nm) facilitated by Förster energy transfer between the visible dye DCM, and the infra-red dye LD800, enabling pump wavelengths anywhere between 420 and 532 nm to be used. These results demonstrate that small and low-cost tuneable visible to near-infrared laser sources are achievable, using a single common pump source. Such devices are envisaged to have wide-ranging applications including medical imaging (including optical coherence tomography), point-of-care optical medical diagnostics (such as flow cytometry), telecommunications, and optical signatures for security coatings. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

The single-phase brushless doubly-fed machine as a generator for wind turbines

This paper proposes a single-phase variant of the Brushless Doubly-Fed Machine, the Single-Phase BDFM, SPB. Like the BDFM it is a variable speed generator which requires a converter rated at only a fraction of the machine rating, using structure with no brushes. Unlike the BDFM, most of its power is delivered directly into a single-phase grid connection. As such it is a potential replacement for PM generators in small-ish wind turbines, potentially with a much lower cost. In this paper we give details of its operation, with reference to results from the first prototype SPB. We also suggest how it may be controlled, again with experimental results. © 2011 IEEE.

菊小长管蚜的化学防治研究

菊小长管蚜Macrosiphorniella sanborni是昆明地区花卉主要害虫之一，该虫对菊花嫩叶，嫩梢，花柄及花勒为害率达65.0%，个别大棚高达100，损失产量25.0%。室内外用1.8爱福丁3000，2500，2000倍液敌敌畏1500，1000，800倍液进行毒杀试验，爱福丁室内的毒杀率达96.6%，敌敌畏达96.3%；田间爱福丁害83.39%，敌敌畏达80.20%。