The adoption of ecosystem-based adaptation in the international climate agenda

4 p.

A new adaptive marine policy toolbox to support ecosystem-based approach to management

4 p.

Competing Ecosystem services: an Assessment of Carbon and timber in the Tropical forests of Central America

4 p.

Economic Valuation of Ecosystem Services: Conflicts in Classification

4 p.

The Costs of Ecosystem Adaptation: Methodology and Estimates for Indian Forests

34 p.

Classifying Ecosystem Services for Economic Valuation: The case of forest water services

30 p.

Economic Assessment of Forest Ecosystem Services Losses: Cost of Policy Inaction

52 p.

Competing Ecosystem Services: an Assessment of Carbon and Timber in the Tropical Forests of Central America

29 p.

Exploitation of soil biota ecosystem services in agriculture: a bioeconomic approach

24 p.

Towards a Comprehensive Approach to Quantifying and Mapping Ecosystem Services Flows

25 p.

Payments for Water Ecosystem Services in Latin America: Evidence from Reported Experience

27 p.

Sequence-function relationships in E. coli transcriptional regulation

Understanding how transcriptional regulatory sequence maps to regulatory function remains a difficult problem in regulatory biology. Given a particular DNA sequence for a bacterial promoter region, we would like to be able to say which transcription factors bind there, how strongly they bind, and whether they interact with each other and/or RNA polymerase, with the ultimate objective of integrating knowledge of these parameters into a prediction of gene expression levels. The theoretical framework of statistical thermodynamics provides a useful framework for doing so, enabling us to predict how gene expression levels depend on transcription factor binding energies and concentrations. We used thermodynamic models, coupled with models of the sequence-dependent binding energies of transcription factors and RNAP, to construct a genotype to phenotype map for the level of repression exhibited by the lac promoter, and tested it experimentally using a set of promoter variants from E. coli strains isolated from different natural environments. For this work, we sought to ``reverse engineer'' naturally occurring promoter sequences to understand how variations in promoter sequence affects gene expression. The natural inverse of this approach is to ``forward engineer'' promoter sequences to obtain targeted levels of gene expression. We used a high precision model of RNAP-DNA sequence dependent binding energy, coupled with a thermodynamic model relating binding energy to gene expression, to predictively design and verify a suite of synthetic E. coli promoters whose expression varied over nearly three orders of magnitude.

However, although thermodynamic models enable predictions of mean levels of gene expression, it has become evident that cell-to-cell variability or ``noise'' in gene expression can also play a biologically important role. In order to address this aspect of gene regulation, we developed models based on the chemical master equation framework and used them to explore the noise properties of a number of common E. coli regulatory motifs; these properties included the dependence of the noise on parameters such as transcription factor binding strength and copy number. We then performed experiments in which these parameters were systematically varied and measured the level of variability using mRNA FISH. The results showed a clear dependence of the noise on these parameters, in accord with model predictions.

Finally, one shortcoming of the preceding modeling frameworks is that their applicability is largely limited to systems that are already well-characterized, such as the lac promoter. Motivated by this fact, we used a high throughput promoter mutagenesis assay called Sort-Seq to explore the completely uncharacterized transcriptional regulatory DNA of the E. coli mechanosensitive channel of large conductance (MscL). We identified several candidate transcription factor binding sites, and work is continuing to identify the associated proteins.

Ecosystem study Altenwoerth: impacts of a hydroelectric power-station on the River Danube in Austria

The aim of this article is to briefly describe the effects of the Altenwoerth Barrage, on the River Danube, on some physical variables and their consequent effects on water chemistry and the biota of the river. The methods used for biological sampling are summarised, especially those used in the limnological part of the study, and the macroinvertebrate and fish fauna listed. Comparisons are then made between the impounded section of river immediately above the dam and two unimpounded free-flowing sections of the river. Further developments on the Danube are considered.

The changing ecosystem of Lake Victoria, East Africa

Dramatic changes are occurring in the Lake Victoria ecosystem. Two-thirds of the endemic haplochromine cichlid species, of international interest for studies of evolution, have disappeared, an event associated with the sudden population explosion of piscivorous Nile perch (Lates: order Perciformes, family Centropomidae) introduced to the lake some thirty years ago. The total fish yield has, however, increased 5-fold from 1970 to 1990, but this yield is now dominated by just three fish species: the introduced Nile perch (Lates niloticus), Nile tilapia (Oreochromis niloticus), and a small endemic pelagic cyprinid (Rastrineobola argentea); these three have replaced a multispecies fishery. Contemporaneously the lake is becoming increasingly eutrophic with associated deoxygenation of the bottom waters, thereby reducing fish habitats. Conditions appear to be unstable.

Ecosystem exploitation, sustainability and biodiversity: Are they compatible?

This articles offers a basis for describing sustainability and then seeks to place this concept on an energetic basis by reference to recent advances in the understanding of patterns and processes in (mainly pelagic) fresh waters. Finally, by relating these to terrestrial ecosystems, it is shown how their sustainability may be attained through encouraging healthy fresh waters. Features of population succession are taken from observations on phytoplankton ecology.