A simple retroelement based knock-down system in Dictyostelium

Characteristics of DIRS-1 Mediated Knock-Downs __ We have previously shown that the most abundant Dictyostelium discoideum retroelement DIRS-1 is suppressed by RNAi mechanisms. Here we provide evidence that both inverted terminal repeats have strong promoter activity and that bidirectional expression apparently generates a substrate for Dicer. A cassette containing the inverted terminal repeats and a fragment of a gene of interest was sufficient to activate the RNAi response, resulting in the generation of ~21 nt siRNAs, a reduction of mRNA and protein expression of the respective endogene. Surprisingly, no transitivity was observed on the endogene. This was in contrast to previous observations, where endogenous siRNAs caused spreading on an artificial transgene. Knock-down was successful on seven target genes that we examined. In three cases a phenotypic analysis proved the efficiency of the approach. One of the target genes was apparently essential because no knock-out could be obtained; the RNAi mediated knock-down, however, resulted in a very slow growing culture indicating a still viable reduction of gene expression.

Time-series regression models to study the short-term effects of environmental factors on health

Time series regression models are especially suitable in epidemiology for evaluating short-term effects of time-varying exposures on health. The problem is that potential for confounding in time series regression is very high. Thus, it is important that trend and seasonality are properly accounted for. Our paper reviews the statistical models commonly used in time-series regression methods, specially allowing for serial correlation, make them potentially useful for selected epidemiological purposes. In particular, we discuss the use of time-series regression for counts using a wide range Generalised Linear Models as well as Generalised Additive Models. In addition, recently critical points in using statistical software for GAM were stressed, and reanalyses of time series data on air pollution and health were performed in order to update already published. Applications are offered through an example on the relationship between asthma emergency admissions and photochemical air pollutants

Psychometric properties of the Spanish version of the Work Group Emotional Intelligence Profi le-Short version

Resumen tomado de la publicaci??n

Short-term storage and mental speed account for the relationship between working memory and fluid intelligence. 'El almacenamiento a corto plazo y la velocidad mental dan cuenta de la relaci??n entre memoria de trabajo e inteligencia fluida'

Resumen tomado de la publicaci??n

Estructura factorial y consistencia interna de la versi??n espa??ola del Parenting Stress Index-Short Form

Resumen tomado de la publicaci??n

Link to ten short video episodes about groupwork

"Rob, Vikki, Shireen, Muzz and Delia have been randomly selected to work together to develop a presentation entitled 'The barriers to learning'. It's not an easy ride. The following 10 episodes show the journey, from their first meeting through to their impressions of the presentation and working together". Produced by the LearnHigher CETL Three areas covered by the site as follows Listening and Interpersonal Skills - the University of Leeds Oral Presentations - Brunel University Group Work - Bradford University

The value of using short-format podcasts to enhance learning and teaching

This paper presents the findings of a podcasting trial held in 2007-2008 within the Faculty of Economics and Business at the University of Sydney, Australia. The trial investigates the value of using short-format podcasts to support assessment for postgraduate and undergraduate students. A multi-method approach is taken in investigating perceptions of the benefits of podcasting, incorporating surveys, focus groups and interviews. The results show that a majority of students believe they gained learning benefits from the podcasts and appreciated the flexibility of the medium to support their learning, and the lecturers felt the innovation helped diversify their pedagogical approach and support a diverse student population. Three primary conclusions are presented: (1) most students reject the mobile potential of podcasting in favour of their traditional study space at home; (2) what students and lecturers value about this podcasting design overlap; (3) the assessment-focussed, short-format podcast design may be considered a successful podcasting model. The paper finishes by identifying areas for future research on the effective use of podcasting in learning and teaching.

A Short presentation on the offence of 'reckless manslaughter'

A Short presentation on the offence of 'reckless manslaughter'

Short Term Health Shocks and School Attendance: The Case of a Dengue Fever Outbreak in Colombia.

This paper makes use of a short, sharp, unexpected health shock in the form of the 2010 Colombian Dengue outbreak to examine the direct and indirect impact of negative health shocks on behaviour of households in affected areas. Our analysis combines data from several sources in order to obtain a comprehensive picture of the influence of the outbreak, and furthermore to understand the underlying mechanisms driving the effects. Our initial analysis indicates that the outbreak had a substantial negative effect on the health status of adults and adversely affected their ability to function as usual in their daily lives. In our aggregated school data, in areas with high levels of haemorrhagic Dengue we observe a reduction innational exam attendance (last year of secondary school) and on enrolment rates in primary education. Further analysis aims to exploit detailed individual level data to gain a more in depth understanding of the precise channels through which this disease influenced the behaviour and outcomes of the poor in Colombia.

Inmenso estrecho : obstacles to achieving post-inmigration identity change in spanish short fiction. 'Inmenso estrecho: obstáculos para conseguir un cambio en la identidad post-inmigratoria en el relato de ficción español'.

Se realiza un análisis de 'Inmenso estrecho', obra que recopila relatos sobre inmigración escritos por 25 autores y que presenta una panorámica de las reacciones que provoca la inmigración en España. Se reflexiona sobre los distintos relatos y sobre el comportamiento de los personajes y se ponen en relación con los descritos por el psicólogo Salman Akhtar que ha estudiado los procesos de adaptación de los inmigrantes a los países de acogida.

Phonological short term memory in deaf children fitted with a cochlear implant : effects of phonological similarity, word lenght and lipreading cues. 'La memoria fonológica a corto plazo en niños sordos con implante coclear : efectos de la similitud fonológica, la longitud de la palabra y la lectura labial'.

Resumen basado en el de la publicación. Resumen en español

Short vowel sounds.

Está indicado para los niños que están aprendiendo fonética. Se trata de hacer coincidir la letra de la solapa con el sonido vocal medio de la palabra, y de que aprendan a distinguir este mismo sonido pronunciando los profesores muy despacio las palabras, o mediante, la alternancia de consonante/vocal/consonante en la palabra. Los conocimientos y habilidades fónicos se pueden desarrollar con toda la clase, en grupos, o individualmente.

Five-minute activities : a resource book of short activities.

Ofrece más de cien ideas para el aula de lengua extranjera. Las actividades se pueden utilizar en cualquier nivel y adaptarse a cualquier situación de enseñanza, pueden variar según el ritmo y el contenido de la clase y contribuir a: facilitar nuevas actividades ; Ayudar a realizar un breve examen ; realizar prácticas de gramática o vocabulario; ayudar a los estudiantes y profesores a conocerse unos a otros.Tiene bibliografía e índice .

OCR : citizenship studies for GCSE short and full courses. 'OCR : estudios de ciudadanía para cursos breves y completos de GCSE'.

Cumple con los requisitos del programa de estudios sobre Ciudadanía en la etapa 4 de secundaria (key stage 4) y, también, sirve para la preparación de varios cursos del título General Certificate Secondary Education (GCSE), concedido por el organismo OCR. Los objetivos de este programa son: dar las habilidades y conocimientos, que afectan a la gente en su vida cotidiana, como el derecho, la política y la economía, para ayudarles a entender mejor lo que pasa en la sociedad y en el complicado mundo en que vivimos. Y, también, ofrecer oportunidades para el debate, la acción práctica y la investigación. El libro está dividido en doce temas estructurados así: el estudio de un caso, basado en hechos reales, seguido de preguntas para la discusión y el debate, explicaciones y detalles de fondo y, por último, definiciones de palabras y de conceptos clave.