Estudo da oxidação da melatonina e de seus produtos por radicais peroxila

Melatonin is an endoleamine that has anti-inflammatory, immunomodulating and antioxidants properties. But there is a contradiction between the antioxidant effects of melatonin and glutathione (GSH). Therefore, the main objective of this work was to study the effect of melatonin on the oxidation of GSH and the effect of GSH on the oxidation of melatonin by peroxyl radicals generated by thermolysis of 2,2 -Azobis(2- amino-propane)-dihydrochloride (AAPH). The influence of the reaction conditions and the identity of the products of oxidation were also studied. The main products obtained during the oxidation of melatonin were its monohydroxylated derivative and N1-acetyl- N2-formyl-5-methoxykynuramine (AFMK), which is the product obtained by oxidative cleavage of the melatonin indole ring. By studying the buffer type, pH and the presence or absence of dissolved oxygen in the reaction system, it was observed that, the yield of AFMK was higher when the pH or the concentration of oxygen was increased. Comparing the reactivity of both molecules GSH and melatonin, it can be seen that intermediates radicals generated during the oxidation of melatonin are able to oxidize GSH itself. We propose that this chemical property could justify the recent reports that demonstrated the inability of melatonin to inhibit the oxidation of GSH in cells

Tubo digestório de Urostreptus atrobunneus (Diplopoda): estrutura e função

Millipedes are macro-arthropods of soil which perform a important role in maintaining the balance of the ecosystem, since they feed on decaying organic matter and because they promotes the soil enrichment, aeration and humidification. These animals are usually found in humid with low light places, under rocks and trunks. The diplopods exoskeleton is much resistant and impregnated with calcium salts. Due the secretion of defense, with strong and unpleasant smell, the animals of this class have few or no predators. Large diplopods populations are not observed, but explosions population can occur for unbalances enviromnmental, by climate change and by use of pesticides that may eliminate potential competitors. In this sense, the millipede Urostreptus atrobrunneus Pierozzi & Fontanetti, 2006, has presented points of infestation in urban centers of Sao Paulo state, causing many disorders of the human population. Because it is a newly described species, little is known of its aspects biological and morphological. Thus, the purpose of this study was to describe the structure and function of the digestive tract of the species U. atrobrunneus by means of histological, histochemistry and ultrastructural techniques. The results demonstrated that the digestive tract of U. atrobrunneus is similar to the descriptions given for other species. The foregut consists of a simple epithelium, composed of cells of different heights, covered by a cuticular intima, leaning on a thin basal membrane, followed by a well-developed muscular layer and an external membrane, around the foregut have a pair of salivary glands. The midgut epithelium has a pseudostratified, supported by a thick basal membrane, followed by a muscular layer and a layer of hepatic cells. The muscle is distributed unevenly coated with an external membrane, this portion is marked by the absence of cuticular intima... (Complete abstract click electronic access below)

Morfologia, ultraestrutura e morfometria da glândula adrenal de paca (Cuniculus paca, Linnaeus, 1766) de cativeiro

Pós-graduação em Cirurgia Veterinária - FCAV

Avaliação do potencial tóxico reprodutivo do extrato de Cordia ecalyculata Vell: em ratos Wistar

Pós-graduação em Biociências - FCLAS

Expressão gênica e proteica da cox-2 e c-Kit em neoplasias mamárias de cadelas

Pós-graduação em Medicina Veterinária - FCAV

Neoplasias mamárias de cadelas: expressão gênica e proteica da via Wnt/b-catenina, sua associação com a transição epitélio-mesênquima e prognóstico

Pós-graduação em Medicina Veterinária - FCAV

Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento

Pós-graduação em Cirurgia Veterinária - FCAV

Cytochemical study of Rhodnius neglectus and Rhodnius prolixus salivary gland cells (Hemiptera, Triatominae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Female paraurethral prostate gland in Bitches

Even though the presence of the female prostate has been reported in many species, including humans, bats and several rodents, it has many anatomical and histological variations. There is still plentiful discussion on the biological function of this organ. Many authors state that paraurethral ducts and glands are functional and homologous to the male prostate. The use of experimental models and a better knowledge of the female prostate gland in other species, can be useful to veterinary medicine as well as human medicine. Therefore the aim of this study is to check for the presence of this gland in female dogs of various breeds and age. For that purpose 25 urethras, from the bladder to the vulva, were collected, fixed in 10% neutral buffered formalin, routinely processed and sectioned into 4 slides of 4 µm, each with 40 µm gap between each set of 4 slides, using an automatic microtome and stained with Hematoxylin and Eosine (HE). The HE sections were evaluated for the presence of prostatic gland in the sample. Unstained tissue sections cut from paraffin blocks were marked with a polyclonal anti-PSA primary antibody. The prevalence of the gland was 32% (8/25). The structure of the paraurethral PSA-positive gland was acinar, organized in buds, with secretory epithelium varying from cubic to columnar; eccentric nuclei, with lose chromatin and a layer of basal cells, very similar to the male prostate were observed. In view of these characteristics, for the first time in the literature, was demonstrate that those glands. may be considered as female prostate in dogs, as they are in other vertebrates

Morphology of Richards' gland in the swarm-founding wasp (Hymenoptera, Vespidae)

Richards' gland is known for the majority of Epiponini wasps, and despite few experimental evidences, the taxonomic distribution in swarm-founder species and the function of this gland remain rather unclear. This work presents a morphological description of Richards' gland in Protonectarina sylveirae. The gland is formed by a cluster of class 3 cells underneath the anterior margin of the fifth metasomal sternite, and a reservoir formed by the intersegmental membrane between the fourth and fifth metasomal sternites where the secretion can be stored. The secretory cells contain a branched end apparatus that carries the secretory products towards the duct cell. Externally, the cuticle of the sternite, where the duct cells penetrate, is characterized by modifications as scales with very numerous pores. The presence of Richards' gland according to the model proposed by Samacá et al. 2013 in Protonectarina corroborates the single origin of this gland in Epiponini. The occurrence of a Golgi apparatus and smooth endoplasmic reticulum suggests pheromone production.

Mecanismo de ação do estrógeno no gene Amphiregulin em células MCF7 e MDA-MB-231 via P13K

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Intrauterine exposure to diesel exhaust diminishes adult ovarian reserve

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos in vitro e in vivo do extrato de glândulas salivares de fêmeas de carrapatos Rhipicephalus sanguineus com 2 dias de alimentação sobre modelos tumorais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação colostral de cabras acometidas por mastite e sua influ~encia nos níveis imunes séricos de cabrito: Jefferson Filgueira Alcindo. -

Pós-graduação em Ciência Animal - FMVA

Use of salivary biomarkers for diagnosis of periodontal disease activity: a literature review

Periodontal disease is an infectious disease characterized by the connective tissue destruction and consequent alveolar bone loss in response to plaque accumulation on the tooth surface. The clinical diagnosis of periodontal disease is based both on clinical examination involving the evaluation of probing depth and radiographic examination of alveolar bone loss but these examinations are not enough to determine the activity of the disease process. For that reason, it has been proposed to seek predictive disease markers in an attempt to assess the disease activity and so, evaluate the efficacy of the periodontal disease treatment. The aim of this review is to present recent advances in the development of proteomic, genomics and microbial biomarkers and potential clinical applications. It was concluded that periodontal treatment based on assessing the levels of salivary biomarkers emerges as a promising method in near future and will become an integral part of the evaluation of periodontal health.