大蹼铃蟾抗菌肽22 对膀胱癌细胞株HLA-ABC以及DR表达影响的对照实验研究

　目的　探讨大蹼铃蟾抗菌肽22 (Maximin22) 对膀胱癌 细胞株HLA2DR 及HLA2ABC 表达的影响, 并与TNF2α、 IFN2α进行比较。方法　采用肿瘤细胞培养方法,流式细胞 仪检测各实验样品对膀胱癌细胞株T24 、BIU287 、SCaBER 的HLA2DR 及HLA2ABC 表达的影响。结果　Maximin22 对 不同的膀胱癌细胞株在小剂量下即有抑制作用,并呈剂量相 关性,各实验样品未见对各膀胱癌细胞株的HLA2DR 表达 有影响,Maximin22 、TNF2α对HLA2ABC 的表达均无影响; IFN2α则对HLA2ABC 的表达有上调作用。结论　Maximin2 2 、TNF2α抗癌机制与提高肿瘤细胞HLA2DR、ABC 的表达无 关, IFN2α可通过提高肿瘤细胞HLA2ABC 的表达提高T 细 胞对膀胱肿瘤的识别杀伤能力。

大蹼铃蟾抗菌肽- 2 与IFN2α22b 和TNF2α对膀胱癌细胞株抑制活性的实验研究

目的:比较大蹼铃蟾抗菌肽- 2 (Maximin 2) 与肿瘤坏死因子2α(TNF2α) 、干扰素2α22b ( IFN2α22b) 对不同膀胱癌细胞株的抑制杀伤作用。方法:采用细胞培养及MTT 比色法,检测Maximin 2 、TNF2α及 IFN2α22b 对3 个不同膀胱癌细胞株的抑制作用及作用特点,计算量效回归曲线及50 %抑制率。结果: Maximin 2 对不同的膀胱癌细胞株在100μgPmL 左右(48 h) 即达到50 %抑制率浓度( IC50) ,并呈剂量相 关性,与TNF2α、IFN2α22b 抑制作用的表现形式不同。Maximin 2 抑制率最高表现在第48 h ,以后逐渐减 弱, TNF2α、IFN2α22b 则在前24 h 作用较弱,以后逐渐增强。结论:Maximin 2 对不同的膀胱癌细胞株均 有较强的抑制作用,同TNF2α、IFN2α22b 比较其作用机制有所不同,Maximin 2 对膀胱癌细胞株的杀伤作 用可能主要是通过对肿瘤细胞的直接抑制,并且相对容易失活。

Water quality parameters of coastal shrimp farms from southwest and southeast regions of Bangladesh

The impacts of shrimp farming on water quality and effluent loading of shrimp farms in southwest (Khulna) and southeast (Cox's Bazar) regions of Bangladesh was investigated during March-August and August-October season, respectively. Water salinity fluctuated from 3.0 to 15.0 ppt in the southwest, whereas it was between 2.5 to 20.0 ppt. in southeast region. Total ammonia nitrogen as recorded in most farms of Cox's Bazar region was higher (0. 1160.438 mg/L) than the recommended level of shrimp farming. Mean values of total ammonia nitrogen and total nitrogen at the outlet of shrimp farms were higher than those of inlet in both regions. Mean values of phosphate phosphorus and total phosphorus at outlet were lower than inlet except in harvest time of Penaeus monodon. Total suspended solids were deposited on the bottom of shrimp farms in both regions, which resulted in higher concentration in inlets than outlets in both regions.

Flow of wet powder in a conical centrifugal filter-an analytical model

A one-dimensional analytical model is developed for the steady state, axisymmetric, slender flow of saturated powder in a rotating perforated cone. Both the powder and the fluid spin with the cone with negligible slip in the hoop direction. They migrate up the wall of the cone along a generator under centrifugal force, which also forces the fluid out of the cone through the powder layer and the porous wall. The flow thus evolves from an over-saturated paste at inlet into a nearly dry powder at outlet. The powder is treated as a Mohr-Coulomb granular solid of constant void fraction and permeability. The shear traction at the wall is assumed to be velocity and pressure dependent. The fluid is treated as Newtonian viscous. The model provides the position of the colour line (the transition from over- to under-saturation) and the flow velocity and thickness profiles over the cone. Surface tension effects are assumed negligible compared to the centrifugal acceleration. Two alternative conditions are considered for the flow structure at inlet: fully settled powder at inlet, and progressive settling of an initially homogeneous slurry. The position of the colour line is found to be similar for these two cases over a wide range of operating conditions. Dominant dimensionless groups are identified which control the position of the colour line in a continuous conical centrifuge. Experimental observations of centrifuges used in the sugar industry provide preliminary validation of the model. © 2011 Elsevier Ltd.

Measurement-Integrated simulations and Kalman filter applied to a co-flowing jet

This paper deals with the experimental evaluation of a flow analysis system based on the integration between an under-resolved Navier-Stokes simulation and experimental measurements with the mechanism of feedback (referred to as Measurement-Integrated simulation), applied to the case of a planar turbulent co-flowing jet. The experiments are performed with inner-to-outer-jet velocity ratio around 2 and the Reynolds number based on the inner-jet heights about 10000. The measurement system is a high-speed PIV, which provides time-resolved data of the flow-field, on a field of view which extends to 20 jet heights downstream the jet outlet. The experimental data can thus be used both for providing the feedback data for the simulations and for validation of the MI-simulations over a wide region. The effect of reduced data-rate and spatial extent of the feedback (i.e. measurements are not available at each simulation time-step or discretization point) was investigated. At first simulations were run with full information in order to obtain an upper limit of the MI-simulations performance. The results show the potential of this methodology of reproducing first and second order statistics of the turbulent flow with good accuracy. Then, to deal with the reduced data different feedback strategies were tested. It was found that for small data-rate reduction the results are basically equivalent to the case of full-information feedback but as the feedback data-rate is reduced further the error increases and tend to be localized in regions of high turbulent activity. Moreover, it is found that the spatial distribution of the error looks qualitatively different for different feedback strategies. Feedback gain distributions calculated by optimal control theory are presented and proposed as a mean to make it possible to perform MI-simulations based on localized measurements only. So far, we have not been able to low error between measurements and simulations by using these gain distributions.

Ecological impact of Mahshahr petrochemical activities on abundance and diversity of macrobenthic fauna in Zangi Creek (Persian Gulf)

The Moosa Creek extends from its opening into the Persian Gulf, with some sub narrow creeks leading to it. Zangi creek is one of the main branches of Moosa creek. The creek contains numerous sources of organic pollution, including sewage outlet flows and boat waste. After establishing the Petrochemical special Economic Zone (PETZONE) in 1997 near to the Zangi Creek, the pipelines, streets and railway made it distinct from eastern and western parts of this creek. Industrial activities have released sludge and effluents in this creek along these years. A survey of the Zangi creek was performed, assessing water properties, organic pollution, and the population density, distribution and diversity of macrobenthic fauna through bi-monthly sampling from July 2006 to September 2007. Samples were collected from water near the bottom and sediment at 7 stations include 2 stations inside the distinct Zangi creek and 4 stations along a transect with 1 km distances between them in eastern free part and one reference station located at the Persian Gulf entrance to the Moosa creek. The environmental parameters such as temperature, salinity, pH, dissolved oxygen, COD, turbidity, EC and heavy metals include Hg, Cd, Pb, Ni as well as percentage silt-clay and total organic matter of the sediment were measured. The faunal population density and their distribution are discussed in relation to the environmental changes. Results showed spatial heterogeneity in faunal distribution of the Zangi creek. Nine groups of macrofauna were identified out of distinct zangi creek. Polychaets formed the dominant group (48%) followed by bivalves (13%), gastropods (10%), Decapods (2%), Tanaids (5%), and all other groups (22%). The distinct creek was heavily polluted without any macrofauna communities probably as a consequence of the high pH, COD, low salinity and heavy metals contamination specially Cd and Pb. The other stations near to the disposal site were found with macrofauna communities commonly tolerant to organic pollution, At 3 km east of the disposal site, macrofauna is comparable to the surrounded creek, whereas macrofauna still indicate environmental degradation. Farther a way, faunal density decreases and equilibrium taxa gradually replace opportunistic species, while the other stations were far from polluted area contained lower pollution and relatively healthy macrofauna. The mean biomass of macrobenthic fauna were estimated for the whole studied area. The results are considered in Minimum density and biomass in surrounded creek and maximum density and biomass in 3 km of surrounded area. Biodiversity Indices were low in surrounded creek. The Shanon-weaver information index was used to describe the spatially variations in diversity. Macrofauna density, shanon and simpson index were significantly variable between surrounded and free parts of Zangi creek (p<0.05). The numerical abundance of macrobenthose varied from 221. m-2 in polluted area to 4346 m-2 in free part of Zangi creek. The Shanon-weaver information index varied from 0.4 in distinct area to 2.9 in reference station. The physico- chemical changes between distinct and free creeks showed significant variations such as pH, salinity and EC. Salinity and EC were significantly positive correlate to macrofauna density, whereas pH and TOM percentage indicated significantly negative correlation to density. Heavy metals concentrations in sediments were higher than water samples. Concentration pattern of heavy metals in sediments and water samples were Ni>Pb>Cd>Hg. Salinity and pH were significantly correlated to metals in sediments (p<0.01). No significant correlation were found between Macrofauna density and heavy metals (p<0.05).

大肠阻塞模型绵羊血浆及组织中血管紧张素-Ⅱ(AⅡ)含量的变化

为研究大肠阻塞动物血浆及组织中A Ⅱ含量的变化,研究以绵羊为实验动物,进行结肠结扎作为大肠阻塞 模型动物,用放射免疫法对其血浆和组织中的A Ⅱ含量进行了测定。结果发现:模型组绵羊动物血浆A Ⅱ含量在术 后呈上升趋势,从第2 天开始显著高于手术前及对照组( P < 01001) ,在术后第2 天的密集采样中低于手术前和对照 组( P < 0105) ,第4 天显著低于手术前和对照组( P < 0101) ;模型组动物右心室和结肠结扎点后段A Ⅱ含量降低( P < 0105) 。结果提示:大肠阻塞动物血浆A Ⅱ含量随病情的发展而迅速升高,与病情的严重程度呈明显正相关。

Taxonomic review of the genus Sinocrossocheilus Wu (Teleostei : Cyprinidae), with a description of four new species

All Sinocrossocheilus species, except S. microstomatus, are reviewed. Four new species, S. labiata, S. papillolabra, S. nigrovittata, and S. longibulla, are described. The genus Sinocrossocheilus differs from other genera of Cyprinidae by the last simple dorsal fin ray being unserrated and unossified, the last unbranched anal fin ray being unserrated and unossified, the 5-branched anal fin rays, the mouth gap being inferior, the rostral cap covering the lower jaw and connecting directly with the lower lip, a row of fleshy lobes on the lower jaw, and a cloudy black spot above the pectoral fin. Sinocrossocheilus labiata is small and has 22 predorsal scales; S. longibulla has a very large air bladder; S. papillolabra possesses a well-developed ventral fin and a wide band covered by fleshy papillae on the lower lip; and S. nigrovittata possesses black longitudinal stripes along the lateral line. Crossocheilus bamaensis and Crossocheilus liuchengensis are transferred to the genus Sinocrossocheilus. Sinocrossocheilus species are endemic to the central and eastern Yunnan-Guizhou Plateau of China, where river systems are anfractuous, including seasonal rivers, cave rivers, underground rivers, and streamlets between mountains. These separated rivers probably provide conditions for the allopatric speciation of the Sinocrossocheilus.

Schistura cryptofasciata, a new loach (Cypriniformes : Balitoridae) from Salween drainage in Yunnan, Southwestern China

Schistura cryptofasciata is distinguished from its congeners by following combination characters: lateral line complete; 8(1)/(2) branched dorsal-fin rays; 9+8 branched caudal-fin rays; no sexual dimorphism; 8-11 indistinct bars, wider than interspaces; caudal-peduncle length 44.1-16.5% standard length, 68.1-90.4% dorsal head length; anterior portion of body scaleless; air bladder entirely embedded in bony capsule; lateral head length 104-122% dorsal head length; lower jaw with a notch at symphysis; lower lip completely interrupted; interorbital width 1.9-2.2 times eye diameter; pelvic-fin origin ventral to dorsal-fin origin; anus 1.0-1.5 eye diameters in front of anal-fin origin; the widest part of black basal caudal bar is the midlateral, and then the upper and lower ends.

Aquaculture provisions of the proposed fishery code

A list is given of the provisions for aquaculture in the Philippine Fishery Code, passed by Congress on its third and final reading on 5 August 1997, under the following headings: 1) Code of practice for aquaculture; 2) Fishpond lease agreements; 3) Fish pens, fish cages, fish traps, etc.; 4) Non-obstruction to navigation and to defined migration paths of fish; 5) Insurance; and, 6) Registration.

Analysis of competition in the wholesale and retail markets for fish in Kisumu, Kenya

This study analyses competition in the wholesale and retail fish marketing system in Kisumu, which is Kenya's largest fish market. It is based on cross sectional and time series primary data collected in a survey involving 88 retailers and 47 wholesale traders of fish in the town. Stratified random sampling method was used in selecting the respondents, Concentration ratios, Lorenz curves and Gini coefficients are derived and evaluated for both markets. They demonstrate that market shares are unequally distributed among the wholesalers and retailers. The Gini coefficients are 0.37 and 0.45 for the whole and retail markets respectively. Based on a Gini coefficient cut-off level of 0.4, it is concluded that the wholesale fish market exhibits effective competition while the retail outlet has oligopolistic tendencies. The implication of this level of competition to price efficiency is discussed. Intervention measures to enhance competition in the market are recommended.

Flow field of a model gas turbine swirl burner

The flow field of a lab-scale model gas turbine swirl burner was characterised using particle imaging velocimetry (PIV) at atmospheric condition. The swirl burner consists of an axial swirler, a twin-fluid atomizer and a quartz tube as combustor wall. The main non-reacting swirling air flow without spray was compared to swirl flow with spray under unconfined and enclosed conditions. The introduction of liquid fuel spray changes the flow field of the main swirling air flow at the burner outlet where the radial velocity components are enhanced. Under reacting conditions, the enclosure generates a corner recirculation zone that intensifies the strength of the radial velocity. Comparison of the flow fields with a spray flame using diesel and palm biodiesel shows very similar flow fields. The flow field data can be used as validation target for swirl flame modeling. © (2013) Trans Tech Publications, Switzerland.

Aeroacoustic source measurement methods for characterizing the sound generated by ducted flow devices with higher-order modes.

The International Organization for Standardization (ISO) method 5136 is widely used in industry and academia to determine the sound power radiated into a duct by fans and other flow devices. The method involves placing the device at the center of a long cylindrical duct with anechoic terminations at each end to eliminate reflections. A single off-axis microphone is used on the inlet and outlet sides that can theoretically capture the plane-wave mode amplitudes but this does not provide enough information to fully account for higher-order modes. In this study, the "two-port" source model is formulated to include higher-order modes and applied for the first three modes. This requires six independent surface pressure measurements on each side or "port." The resulting experimental set-up is much shorter than the ISO rig and does not require anechoic terminations. An array of six external loudspeaker sources is used to characterize the passive part of the two-port model and the set-up provides a framework to account for transmission of higher-order modes through a fan. The relative importance of the higher-order modes has been considered and their effect on inaccuracies when using the ISO method to find source sound power has been analyzed.

Aerodynamic design of high end wall angle turbine stages-part I: Methodology development

A design methodology is presented for turbines in an annulus with high end wall angles. Such stages occur where large radial offsets between the stage inlet and stage outlet are required, for example in the first stage of modern low pressure turbines, and are becoming more prevalent as bypass ratios increase. The turbine vanes operate within s-shaped ducts which result in meridional curvature being of a similar magnitude to the bladeto-blade curvature. Through a systematic series of idealized computational cases, the importance of two aspects of vane design are shown. First, the region of peak end wall meridional curvature is best located within the vane row. Second, the vane should be leant so as to minimize spanwise variations in surface pressure-this condition is termed "ideal lean." This design philosophy is applied to the first stage of a low pressure turbine with high end wall angles. © 2014 by ASME.

Pseudobagrus fui Miao, a valid bagrid species from the Yangtze River drainage, South China (Teleostei: Bagridae)

Pseudobagrus fui Miao is a valid bagrid species that can be separated from all its congeners in having 27-33 anal-fin rays. Among the species of Pseudobagrus, it forms part of a group of approximately 20 species putatively characterized by having a smooth anterior margin of the pectoral-fin spine and short maxillary barbels not extending to the base of the pectoral-fin spine. Pseudobagrus fui, together with P. nitidus and P. vachelli, can be differentiated from all other fork-tailed species of this group by having no fewer than 20 branched anal-fin rays, the posterior end of the anal-fin base posterior to the vertical through the posterior end of the adipose-fin base, and anal-fin base longer than the adipose-fin base. It further differs from P. nitidus in having a gas bladder without beaded lateral and posterior margins, and from P. vachelli in having short maxillary barbels not extending to the base of the pectoral-fin spine. Pseudobagrus fui is currently known from the main stream of the upper Yangtze River and its tributaries, the Min River, Jialing River, Tuo River, Wu River and Chishui River in Sichuan Province, Guizhou Province, and the Chongqing City. The identity and nomenclature of Pseudobagrus nitidus is also discussed.