Executive Order No. 1, February 27, 1961

Iowa Liquor Commission is directed to coordinate with the State Car Dispatcher the merger of various IBM equipment.

Executive Order No. 2, February 27, 1961

State Car Dispatcher is directed to coordinate with the Iowa Liquor Commission the merger of various IBM equipment.

Executive Order No. 3, March 1, 1961

Informing several departments that approval for the change in data processing equipment should be approved in writing from the State Comptroller prior to submission to the Executive Council.

Executive Order No. 4, March 30, 1961

Requesting that all Department Heads of all Agencies of the State Government compile an inventory of all property in their deparments or under the control of their department.

Executive Order No. 5, April 11, 1961

Requesting all state departments having state automobiles stationed and operated in the Des Moines area have them inspected at the Des Moines Municipal Car Inspection Station.

Executive Order No. 6, February 21, 1962

Approved appointments of Judges are made.

Executive Order No. 7, April 3, 1962

Creates several Governor's Commissions effective January 12, 1961 until January 11, 1963

Executive Order Number Twenty, December 16, 2009

The people of Iowa expect that their state government will efficiently provide services and will be accountable in the use of taxpayer dollars.

High order hydrodynamical equations

Hydrodynamical equations act as a link between the local observed magnitudes of galactic motion and the general ones accounting for the behaviour of the Galaxy as a whole. Constraints are set usually in order to use them even in the lower order hierarchy. The authors present in this paper the complete expressions up to their fourth order. These equations will be used in the next future in their general form taking into account both the expected increase of kinematic data that the astrometric mission Hipparcos will provide, and some recent results indicating the possibility to obtain estimates for the momenta gradients.

Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein

Gene duplication was prevalent during hominoid evolution, yet little is known about the functional fate of new ape gene copies. We characterized the CDC14B cell cycle gene and the functional evolution of its hominoid-specific daughter gene, CDC14Bretro. We found that CDC14B encodes four different splice isoforms that show different subcellular localizations (nucleus or microtubule-associated) and functional properties. A microtubular CDC14B variant spawned CDC14Bretro through retroposition in the hominoid ancestor 18-25 million years ago (Mya). CDC14Bretro evolved brain-/testis-specific expression after the duplication event and experienced a short period of intense positive selection in the African ape ancestor 7-12 Mya. Using resurrected ancestral protein variants, we demonstrate that by virtue of amino acid substitutions in distinct protein regions during this time, the subcellular localization of CDC14Bretro progressively shifted from the association with microtubules (stabilizing them) to an association with the endoplasmic reticulum. CDC14Bretro evolution represents a paradigm example of rapid, selectively driven subcellular relocalization, thus revealing a novel mode for the emergence of new gene function

Phosphorylation modulates FOXC2 transcriptional program by controlling the high-order interaction with DNA.

Phosphorylation of transcription factors is a rapid and reversible process linking cell signaling and control of gene expression, therefore understanding how it controls the transcription factor functions is one of the challenges of functional genomics. We performed such analysis for the forkhead transcription factor FOXC2 mutated in human hereditary disease lymphedemadistichiasis and important for the development of venous and lymphatic valves and lymphatic collecting vessels. We found that FOXC2 is phosphorylated in a cell-cycle dependent manner on eight evolutionary conserved serine/threonine residues, seven of which are clustered within a 70 amino acid domain. Surprisingly, the mutation of phosphorylation sites or a complete deletion of the domain did not affect the transcriptional activity of FOXC2 in a synthetic reporter assay. However, overexpression of the wild type or phosphorylation-deficient mutant resulted in overlapping but distinct gene expression profiles suggesting that binding of FOXC2 to individual sites under physiological conditions is affected by phosphorylation. To gain a direct insight into the role of FOXC2 phosphorylation, we performed comparative genome-wide location analysis (ChIP-chip) of wild type and phosphorylation-deficient FOXC2 in primary lymphatic endothelial cells. The effect of loss of phosphorylation on FOXC2 binding to genomic sites ranged from no effect to nearly complete inhibition of binding, suggesting a mechanism for how FOXC2 transcriptional program can be differentially regulated depending on FOXC2 phosphorylation status. Based on these results, we propose an extension to the enhanceosome model, where a network of genomic context-dependent DNA-protein and protein-protein interactions not only distinguishes a functional site from a nonphysiological site, but also determines whether binding to the functional site can be regulated by phosphorylation. Moreover, our results indicate that FOXC2 may have different roles in quiescent versus proliferating lymphatic endothelial cells in vivo.

Parental post-traumatic reactions after premature birth: implications for sleeping and eating problems in the infant.

BACKGROUND: Progress in perinatal medicine has made it possible to increase the survival of very or extremely low birthweight infants. Developmental outcomes of surviving preterm infants have been analysed at the paediatric, neurological, cognitive, and behavioural levels, and a series of perinatal and environmental risk factors have been identified. The threat to the child's survival and invasive medical procedures can be very traumatic for the parents. Few empirical reports have considered post-traumatic stress reactions of the parents as a possible variable affecting a child's outcome. Some studies have described sleeping and eating problems as related to prematurity; these problems are especially critical for the parents. OBJECTIVE: To examine the effects of post-traumatic reactions of the parents on sleeping and eating problems of the children. DESIGN: Fifty families with a premature infant (25-33 gestation weeks) and a control group of 25 families with a full term infant participated in the study. Perinatal risks were evaluated during the hospital stay. Mothers and fathers were interviewed when their children were 18 months old about the child's problems and filled in a perinatal post-traumatic stress disorder questionnaire (PPQ). RESULTS: The severity of the perinatal risks only partly predicts a child's problems. Independently of the perinatal risks, the intensity of the post-traumatic reactions of the parents is an important predictor of these problems. CONCLUSIONS: These findings suggest that the parental response to premature birth mediates the risks of later adverse outcomes. Preventive intervention should be promoted.

Executive Order Number Twenty-One, February 1, 2010

Orders that the Executive Director of the Iowa Finance Authority, shall serve as the Governor’s designee related to the issuance of Qualified Midwestern Disaster Area Bonds.

Executive Order Number Twenty-Two, February 3, 2010

Requests all state departments and agencies to consider using Project Labor Agreements on large-scale construction projects to provide structure and stability, promote efficient, on-time completion and ensure the high standards and reasonable costs on the projects in order to move Iowa’s economy forward.