Comportamento de oviposição em Chrysomya megacephala (F.) (Diptera: Calliphoridae): distinção quantitativa frente a posturas prévias?

Chrysomya megacephala (Fabricius), has considerable medical, veterinary and forensic importance. In insects in general, the ability to track the food substrate is due to highly sensitive organs adapted for detecting odors, particularly in the case of blowflies, which is one of carcass main consumers. Eggs of blowflies are usually aggregated in function of the behavior of other pheromone mediated by females laying eggs in place on the same substrate. One of the main reasons for studying oviposition behavior is because it may indirectly affect individual qualification, population dynamics and community structure. It has been suggested that female blowflies can evaluate the number of eggs on a substrate, decreasing the size of oviposition in very saturated substrates with larvae or eggs leading to a lower intraspecific competition. The present study investigated the oviposition behavior of females of C. megacephala, and wished to consider whether they are able to distinguish quantitatively (size of the mass) eggs previously put into a place of posture and hence indicate whether there is a limit beneficial (to avoid competition among larvae) for its oviposition on the same substrate or if that place would already be saturated, requiring find another substrate without eggs or less for futures ovipositions. With 20 females and five males, five treatments were applied, repeated ten times and analyzed using the Mann-Whitney test. Each treatment consists in placing, inside the cage, a flask with 50g of ground beef and another one in the other side, with the same amount of substrate with a certain amount of prior oviposition for a period of 5 hours. The masses prior eggs were used 0.03 g, 0.05 g, 0.1 g, 0.2 g and 0.3 g (respectively, T1, T2, T3, T4 and T5). The other part of the work consisted in the use of a “Y” olfactometer, a device used to determine the choice of an invertebrate against... (Complete abstract click electronic access below)

Ação de topiramato sobre o desenvolvimento larval de Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae)

A Entomologia Forense é um ramo de investigação científica que aplica o estudo de insetos e outros artrópodes a procedimentos legais e está se desenvolvendo e chamando bastante atenção nos últimos anos. Diversos estudos vêm sendo realizados nesta área para que os profissionais responsáveis possuam o maior número de informações possível sobre os insetos utilizados para estes fins, permitindo-lhes assim fazer melhores análises nos casos litigiosos envolvendo a descoberta da causa até a estimativa de tempo do intervalo pós-morte (I.P.M.) em um cadáver humano. Dentro deste ramo de estudo, a ordem Diptera apresenta certo destaque por apresentar diversos insetos com hábitos necrófagos, como por exemplo a família Calliphoridae, que se destaca pela grande distribuição e número de registros da presença de seus representantes nos tecidos de corpos animais no início da decomposição. A espécie Chrysomya megacephala (Fabricius), pertencente a esta família, tendo sido introduzida acidentalmente aqui no Brasil alguns anos atrás, possui uma importância médico-sanitária como veiculadora de patógenos, eventual causadora de miíases e, pelo fato de colocar seus ovos sobre tecidos de animais em decomposição, é uma importante espécie utilizada em estudos forenses. Baseando-se em trabalhos já realizados, sabe-se que a presença de determinadas substâncias químicas no substrato alimentar das larvas destas moscas pode alterar seu desenvolvimento, e sabendo quais seriam as mudanças provocadas por uma dessas substâncias, a análise do cadáver se tornaria mais completa e confiável durante a estimativa do I.P.M. A área responsável pelo estudo da interação entre substâncias químicas e os seus efeitos nos insetos é chamada de Entomotoxicologia, que também permite detectar traços de drogas lícitas ou não no trato digestivo de insetos necrófagos... (Resumo completo, clicar acesso eletrônico abaixo)

Análise Isotópica de Carbono-13 e Nitrogênio-15 da Ipomoea violacea para Determinação de sua Origem Geográfica

Along the Earth globe we can find many types of psychoactive plants. Among them is the Ipomoea violacea, popularly known as Morning Glory. There are ergotalkaloids producer associated-fungus in its leaves and seeds. One of these alkaloids that can be found is the ergine (or LSA), a homologous substance of the lysergic acid diethylamide (LSD). There are many discussions around the world about the inclusion of LSA in the list of controlled substances. In Brazil, this was recently prohibited. One of the most important point of view in the study of isotopic composition of 13C and 15N of this plant is the fact that there is a total alkaloid variation in function of its geographic origin like was verified in 1960’s, besides to aggregate knowledge about it. This work was made to verify if the isotopic ratio can be used as a tool in tracing this illegal Brazilian plant. We could conclude that this plant presents a C3 photosynthetic pathway, its parts has different isotopic carbon and nitrogen composition and that stable isotope analysis can be successfully used as a tool to detect its geographic origin

Controle de qualidade e qualificação de fornecedores de matérias primas utilizadas em uma farmácia de manipulação

Este trabalho apresenta uma proposta de qualificação de fornecedores de matérias primas para uma farmácia na cidade de Araraquara, estado de São Paulo. A qualificação de fornecedores é um processo de extrema importância no que diz respeito ao produto acabado e, para que tenha qualidade, essa qualidade deve vir desde as matérias primas que o compõem. A RDC nº 67 de 8 de outubro de 2007 diz que as farmácias devem adquirir matérias primas apenas de fornecedores qualificados. Para o processo de qualificação, a mesma RDC diz que devem ser feitas auditorias e levantamentos de dados dos fornecedores, bem como o controle de qualidade das matérias primas fornecidas. Foram escolhidas quatro matérias primas utilizadas pela farmácia de três fornecedores diferentes. O processo começou com o envio de questionários avaliativos, onde cada fornecedor deveria colocar as informações solicitadas. O passo seguinte é o controle de qualidade das matérias primas. A partir dos dados dos questionários e dos resultados do controle de qualidade, acrescidos de outros fatores como prazo de entrega, compõe-se o Índice de Qualificação do Fornecedor (IQF), que é como uma “nota” que o fornecedor recebe e, através desse índice, pode ser DESQUALIFICADO, QUALIFICADO ou ainda CERTIFICADO. A certificação ocorre após a auditoria do estabelecimento do fornecedor e também entra na composição do IQF. Após o processo de qualificação dos três fornecedores, foi elaborado um Procedimento Operacional Padrão (POP) com todas as etapas que devem ser feitas para a qualificação...

Análise de SNPs do DNA mitocondrial em indivíduos residentes no estado do Espírito Santo para aplicação na Identificação Humana

Pós-graduação em Biotecnologia - IQ

Human identification by means of conventional and digital Cheiloscopy: a study of the literature

One of the areas of human identification is Cheiloscopy, the name given to the study of the lips, their characteristics (such as thickness, position of the grooves and grooves) and the record of the impressions left by them. There are variations in the layout of the lines and fissures of the lips, which are unique to each individual, permanent and unchanging. The lip print rarely changes, enduring minor traumas such as inflammation or sores. In criminal investigations, lip prints, visible through the presence of lipstick, can be found on glasses, napkins, clothes, cigarettes, indicating a relationship between the subject and the scene of the crime. Latent impressions may be revealed employing specific chemicals such as powder of silver and aluminum nitrate. Although it is not a very common method, Cheiloscopy may become very useful in forensics due to the extensive amount of valuable information that it brings. The objective of this study was to review the literature on the use of Cheiloscopy in human identification, using traditional and digital methods. It was found that the literature is still in need of studies in this area. The advent of new digital technologies can facilitate the implementation of technical expertise, generating speed and objectivity. New research studies are necessary, especially in the development of digital methods. The application of Cheiloscopy can greatlyhelp with Law, in the identification of living suspects and dead individuals. In the end the benefit will fall to society as a whole.

Estimativa da altura por dimensões dentárias: uma revisão da literatura

The knowledge of Forensic Anthropology is very importance in cases of identification of human remains. One phase of this process is the study of human height, which can be accomplished with relative ease when intact corpses, complete skeletons or long bones are available. However, the experience of Forensic Dentistry is essential in situations in which there is only information of the skull or of the teeth. The objective of this study was to review in the literature and evaluate works concerning the estimate of the height calculated from dental dimensions. Carrea, in 1920, proposed the estimation of the probable height of an individual by developing formulas for maximum and minimum heights from measurements of the lower central and lateral incisors and canines. The method was used in the case "Josef Mengele", to complement estimates. Tested in the Brazilian population, 70% of match between the actual and the estimated stature were obtained. Using more precise instruments, in the modified method, 96% of correct matching were verified. Recently, a new formula was introduced to estimate height, from measurements of upper teeth, because the technique cannot be used when jaw is not available. The correlation between height and dental dimensions is demonstrated. However, there is still a lack in scientific literature in this field, and further studies are necessary. The estimate of height from dental dimensions can be very useful and important, especially in situations where the complete skeleton it not found, and long bones are not available.

Violence against children and adolescents: the importance of knowledge of the spectrum of bruise colors in its diagnosis

Introduction: One of the violence types more observed against children is the physical abuse, which produces many types of traumatic injuries. Contusions are common trauma signs and may indicate aggression when frequently present in locations where accidental injuries are rare. Contusions in skin undergo color changes with the passage of time, and such change in color is called “spectrum of bruise colors of Legrand du Saulle”. Thus, it is possible to estimate the age of the lesion based on the evaluation of color. Injuries caused by child abuse often reach regions of head, face and neck, because they are exposed and easily accessible. Therefore, the dentist is responsible with regard to the diagnosis and the actions to be undertaken against the cases of child abuse. Objective: To present and discuss the significance of the spectrum of bruise colors as a considerable visible trace in suspicion of violence against children and adolescents, during dentists’ clinical routine. Literature review: The study of the “spectrum of bruise colors of Legrand du Saulle” refers to the estimation of the age of the injury, in respect to the color change. The application of this study is of great value for identify if a lesion occurred from a single incident or from multiple incidents, especially in cases where it occurs in the same region of the body, indicative of repetitive trauma. Conclusion: The spectrum of bruise colors is very important for health professionals, such as dentists, for the identification or suspicion of cases of abuse against children and adolescents. As great part of the lesions originated of abuse involve the areas of head and neck, it is unacceptable that these professionals are unaware of the basic signs to help in early diagnosis, which is the case of the colors of the lesions. However, concerning the chronology of the spectrum of bruise colors, it is not possible to establish rigorous times.

A problemática trazida pelos bancos de perfis genéticos criminais no Brasil

Pós-graduação em Direito - FCHS

Cães e gatos: expressão das lesões em intoxicações criminais

Pós-graduação em Medicina Veterinária - FMVZ

Implantação de gestão da qualidade no serviço de hemoterapia em um hospital público do Estado de São Paulo

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Efeitos de perfurações artificiais no processo de decomposição em Sus Scrofa (L.) e confecção de uma check-list da entomofauna de Diptera visitante, durante inverno e verão, na região sudeste

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

La informática en el consultorio odontológico y el uso de los regsitros digitales

The use of computers in dental clinics has brought many benefits to dentist, helping them in various technical, administrative and legal. This study aimed to verify whether dentist, students of the post-graduate course in Orthodontics and Implantodontia of the Brazilian Association of Dentistry (ABO), regional Araçatuba-SP, using computers and digital documentation in clinical practice. They were invited to participate in the study all 60 students enrolled in courses of Orthodontics and Implantodontia of those institutions, in the year 2007 and those who consented (n = 52) answered a questionnaire containing questions open and closed on the subject . As the profile of the participants, 64.5% are male, 51.9% are between 30-39 years of age and 48.1% completed the graduation in the 90th. A 69.3% said they didn't receive any notion of computing during graduation and 67.3% use the computer in the office, mainly for management of the same (34.3%). Regarding the use of electronic record 55.8% reported using this technology, while 44.2% denied. 32.7% do not believe that electronic records that can serve as proof of judicial and 35.7% believe that the electronic records should be archived for 20 years. It follows that most professionals use the computer in the office and also the digital records, but does not feel secure about the legal validity of such documents, unknown, including the appropriate time of its filing.

Nível de conhecimento dos cirurgiões dentistas sobre a qualidade dos prontuários odontológicos para fins de identificação humana

Objective: The aim of this research was to determine the knowledge level of 400 dentists registered in the Regional Dental Council of Cuiabá, through a questionnaire about the importance of records in the process of human identification. Results: We observed that 48.36% of them dispense between 10 and 20 minutes. Only 13.1% of the surveyed dentists did not have the habit of writing down the oral conditions of their patients before starting treatment and 42.62% have only one odontogram filled. From the 122 participants, 11.11% reported that the documentation had no utility to establish the patients identity. To make the situation worse, 33.6% of them said they did not keep the records of patients updated. Conclusion: We conclude that the dentist of Cuiabá has enough knowledge on preparing, maintaining and importance of dental records before the death in the identification process for establishing the patient identity. But, always he did not properly fills the dental records, thus it has been reducing their clinical, administrative and legal value.

Actitud de los docentes de las facultades de Odontologia Brasilenas ante los aspectos bioeticos, eticos y legales del tratamiento odontológico

The study consist on an analysis of attitude by professors of Brazilian Dental Schools before the aspects bioethical, ethic and legal dental treatment aspects. The data collect occurred by semi-structured questioners send by e-mail and correspondence. It was applied the content analysis, by categorical analysis technique, according to Bardin. About bioethical aspects, 94,6% cited that the decision of treatment should be realized between professional and patient, because it don t interfere on negative form (100%). Everybody consider important the interaction professional/patient, because it establishes the respect and mutual agreement between them (61,8%). Tangent at ethic aspects, 95,5% show all alternatives for determined case, even knowing that can lost patient, explaining on the technique and laical form to clear doubts(61,9%). About legal aspects, 82,4% allege that dental surgeon should treat his patient like consumer whereas offer techniques, materials and know through profession. And, like legal prove on occasional lawsuit, 57,1% mentioned that professional don t need of contract necessarily, but a good documentation about patient and e assigned by him. It concluded that these aspects should be teached always to Dentistry academics, whereas they re inherent at profession, to not stay vulnerable at future ethic, judges and other problems.