Epidermal Growth Factor Impairs Palatal Shelf Adhesion and Fusion in the T gf-β3 Null Mutant

The cleft palate presented by transforming growth factor-β3 (Tgf-β3 ) null mutant mice is caused by altered palatal shelf adhesion, cell proliferation, epithelial-to-mesenchymal transformation and cell death. The expression of epidermal growth factor (EGF), transforming growth factor-β1 ( Tgf-β1 ) and muscle segment homeobox-1 (Msx-1) is modified in the palates of these knockout mice, and the cell proliferation defect is caused by the change in EGF expression. In this study, we aimed to determine whether this change in EGF expression has any effect on the other mechanisms altered in Tgf-β 3 knockout mouse palates. We tested the effect of inhibiting EGF activity in vitro in the knockout palates via the addition of Tyrphostin AG 1478. We also investigated possible interactions between EGF, Tgf-β 1 and Msx-1 in Tgf-β 3 null mouse palate cultures. The results show that the inhibition of EGF activity in Tgf-β 3 null mouse palate cultures improves palatal shelf adhesion and fusion, with a particular effect on cell death, and restores the normal distribution pattern of Msx-1 in the palatal esenchyme. Inhibition of TGF-β 1 does not affect either EGF or Msx-1 expression.

O papel da embalagem – projeto de design de uma embalagem Shelf Ready Packaging/Retail Ready Packaging

A tendência da distribuição moderna para a crescente utilização das embalagens secundárias para exposição dos produtos, aliada à grande importância da embalagem na promoção e venda dos mesmos, leva à necessidade de olhar de outra forma para o design de embalagem secundária Shelf Ready Packaging (SRP)/ Retail Ready Packaging (RRP). O objetivo deste projeto é o desenvolvimento de uma nova metodologia de design de embalagem SRP/RRP para a Cartonarte, Lda., visando a criação de uma nova embalagem SRP/RRP para um cliente relevante desta empresa. A proposta da nova embalagem SRP/RRP desenvolvida tem não só em atenção aspetos técnicos e a gestão de custos de produção e reposição nos retalhistas, como também questões comunicativas como a promoção do produto, a aparência na prateleira e uma boa ligação com o consumidor. A ligação com a embalagem primária (EP) e as questões ligadas à distribuição, como a fácil identificação, transporte e manuseamento, são também prioridades. O design deste tipo de embalagens assume cada vez mais importância, tendo-se conseguido desenvolver um modelo estrutural com pouco desperdício de matéria-prima, sem custos de produção desnecessários, com fácil manuseamento, amigo do consumidor, interligado graficamente à EP, melhorando também aspetos comunicacionais.

Benthic foraminiferal abundances from eight sites of the Northwest Australian shelf

Foraminiferal analysis of Miocene to recent strata of the Northwest Shelf of Australia is used to chart West Pacific Warm Pool (WPWP) influence. The assemblage is typified by "larger" foraminifera with ingressions of the Indo-Pacific "smaller" taxa Asterorotalia and Pseudorotalia at around 4 Ma and from 1.6 to 0.8 Ma. A review of recent and fossil biogeography of these taxa suggests their stratigraphic distribution can be used to document WPWP evolution. From 10 to 4.4 Ma a lack of biogeographic connectivity between the Pacific and Indian Ocean suggests Indonesian Throughflow (ITF) restriction. During this period, the collision of Australia and Asia trapped warmer waters in the Pacific, creating a central WPWP biogeographic province from the equator to 26°N. By 3 Ma Indo-Pacific species migrated to Japan with the initiation of the "modern" Kuroshio Current coinciding with the intensification of the North Pacific Gyre and Northern Hemisphere ice sheet expansion. Indo-Pacific taxa migrated to the northwest Australia from 4.4 to 4 Ma possibly because of limited ITF. The absence of Indo-Pacific taxa in northwest Australia indicates possible ITF restriction from 4 to 1.6 Ma. Full northwest Australian biogeographic connectivity with the WPWP from 1.6 to 0.8 Ma suggests an unrestricted stronger ITF (compared to today) and the initiation of the modern Leeuwin Current. The extinction of some Indo-Pacific species in northwest Australia after 0.8 Ma may be related to the effects of large glacial/interglacial oscillations and uplift of the Indonesian Archipelago causing Indonesian seaway restriction.

Abundance, biomass and secondary production of benthic megafauna on the Barents Sea shelf in 2008 and 2009

Megabenthos plays a major role in the overall energy flow on Arctic shelves, but information on megabenthic secondary production on large spatial scales is scarce. Here, we estimated for the first time megabenthic secondary production for the entire Barents Sea shelf by applying a species-based empirical model to an extensive dataset from the joint Norwegian? Russian ecosystem survey. Spatial patterns and relationships were analyzed within a GIS. The environmental drivers behind the observed production pattern were identified by applying an ordinary least squares regression model. Geographically weighted regression (GWR) was used to examine the varying relationship of secondary production and the environment on a shelfwide scale. Significantly higher megabenthic secondary production was found in the northeastern, seasonally ice-covered regions of the Barents Sea than in the permanently ice-free southwest. The environmental parameters that significantly relate to the observed pattern are bottom temperature and salinity, sea ice cover, new primary production, trawling pressure, and bottom current speed. The GWR proved to be a versatile tool for analyzing the regionally varying relationships of benthic secondary production and its environmental drivers (R² = 0.73). The observed pattern indicates tight pelagic? benthic coupling in the realm of the productive marginal ice zone. Ongoing decrease of winter sea ice extent and the associated poleward movement of the seasonal ice edge point towards a distinct decline of benthic secondary production in the northeastern Barents Sea in the future.

Abundance of tintinnids in waters of the Argentine Shelf and the Drake Passage 2002-2003

The relationship between the abundance and diversity of tintinnids and the concentration of chlorophyll a (Chl a) was contrasted between neritic and oceanic waters of the SW Atlantic during autumn and summer. Chl a and tintinnid abundance and biomass reached maximum values (17.53 µg/L, 2.76 x 10**3 ind./L and 6.29 µg C/L, respectively) in shelf waters during summer, and their mean values generally differed by one order of magnitude between environments. Peaks in species richness (13) and Shannon diversity index (2.12) were found in the shelf-ocean boundary, but both variables showed nonsignificant differences between areas. Species richness correlated significantly with both Chl a and abundance. Such relationships, which followed a negative linear or quadratic function in the shelf and a positive linear function in oceanic waters, are thought to reflect either the competitive dominance of one species or a relatively wide spectrum of tintinnid size-classes, respectively.

Organic carbon isotope ratios of sea surface sediments and sea-ice from the Amerasian Continental shelf

Organic matter origins are inferred from carbon isotope ratios (delta13C) in recent continental shelf sediments and major rivers from 465 locations from the north Bering-Chukchi-East Siberian-Beaufort Sea, Arctic Amerasia. Generally, there is a cross-shelf increase in delta13C, which is due to progressive increased contribution seaward of marine-derived organic carbon to surface sediments. This conclusion is supported by the correlations between sediment delta13C, OC/N, and delta15N. The sources of total organic carbon (TOC) to the Amerasian margin sediments are primarily from marine water-column phytoplankton and terrigenous C3 plants constituted of tundra taiga and angiosperms. In contrast to more temperate regions, the source of TOC from terrigenous C4 and CAM plants to the study area is probably insignificant because these plants do not exist in the northern high latitudes. The input of carbon to the northern Alaskan shelf sediments from nearshore kelp community (Laminaria solidungula) is generally insignificant as indicated by the absence of high sediment delta13C values (-16.5 to -13.6 per mil) which are typical of the macrophytes. Our study suggests that the isotopic composition of sediment TOC has potential application in reconstructing temporal changes in delivery and accumulation of organic matter resulting from glacial-interglacial changes in sea level and environments. Furthermore, recycling and advection of the extensive deposits of terrestrially derived organic matter from land, or the wide Amerasian margin, could be a mechanism for elevating total CO2 and pCO2 in the Arctic Basin halocline.

Variability of marine climate on the North Icelandic Shelf in a 1357-year proxy archive based on growth increments in the bivalve Arctica islandica

A multicentennial and absolutely-dated shell-based chronology for the marine environment of the North Icelandic Shelf has been constructed using annual growth increments in the shell of the long-lived bivalve clam Arctica islandica. The region from which the shells were collected is close to the North Atlantic Polar Front and is highly sensitive to the varying influences of Atlantic and Arctic water masses. A strong common environmental signal is apparent in the increment widths, and although the correlations between the growth increment indices and regional sea surface temperatures are significant at the 95% confidence level, they are low (r ~ 0.2), indicating that a more complex combination of environmental forcings is driving growth. Remarkable longevities of individual animals are apparent in the increment-width series used in the chronology, with several animals having lifetimes in excess of 300 years and one, at 507 years, being the longest-lived non-colonial animal so far reported whose age at death can be accurately determined. The sample depth is at least three shells after AD 1175, and the time series has been extended back to AD 649 with a sample depth of one or two by the addition of two further series, thus providing a 1357-year archive of dated shell material. The statistical and spectral characteristics of the chronology are investigated by using two different methods of removing the age-related trend in shell growth. Comparison with other proxy archives from the same region reveals several similarities in variability on multidecadal timescales, particularly during the period surrounding the transition from the Medieval Climate Anomaly to the Little Ice Age.