997 resultados para Apraxin, L. (17..-18..) -- Portraits
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Échelle(s) : [ca 1:2 440 000], échelle 100 kilomètres [= 4,1 cm]
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Échelle(s) : [ca 1:225 000], 2 myriamètres [= 8,9 cm]
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Échelle(s) : [ca 1:1 852 000], échelle 10 myriamètres [= 5,4 cm]
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Échelle(s) : [ca 1:1 852 000], échelle 10 myriamètres [= 5,4 cm]
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Kirje 11.12.1956
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Addition of insulin, IGF I or IGF II to serum-free cultures of fetal rat brain cells (gestation day 15/16) significantly stimulates DNA synthesis. The dose-response curves show that IGF I is more potent than insulin; half maximal stimulation of [3H]thymidine incorporation is obtained at about 0.4 nM IGF I and 14 nM insulin, respectively. Cultures initiated 2 days later (gestation day 17/18) showed a decreased responsiveness to both peptides. No additive effect was observed after combined addition of both peptides at near-maximal doses. Both peptides show a latency of action of about 12-18 h. In the presence of either IGF or insulin, neuronal as well as glial enzymes are increased, suggesting that neuronal and glial precursor cell division is influenced. IGF I and IGF II interact with a specific binding site for which insulin competes very weakly; however IGF I and IGF II bind with relatively high affinity to the insulin specific binding site. The present results support the hypothesis that both insulin and IGF stimulate mitotic activity by interacting with specific somatomedin receptors and suggest a physiological role of IGF in the developing brain.
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Échelle(s) : [ca 1:1 600 000]
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Échelle(s) : [ca 1:9 500] échelle de mille mètres, 1000 Mètres [= 10,5 cm]
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Échelle(s) : [ca 1:10 000], 1000 mètres [= 10 cm]
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Échelle(s) : [ca 1:3 300], 100 mètres [= 3 cm]