Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

Influenza A virus assembly is an unclear process, whereby individual virion components form an infectious particle. The segmented nature of the influenza A genome imposes a problem to assembly because it requires packaging of eight distinct RNA particles (vRNPs). It also allows genome mixing from distinct parental strains, events associated with influenza pandemic outbreaks. It is important to public health to understand how segmented genomes assemble, a process that is dependent on the transport of components to assembly sites. Previously, it has been shown that vRNPs are carried by recycling endosome vesicles, resulting in a change of Rab11 distribution. Here, we describe that vRNP binding to recycling endosomes impairs recycling endosome function, by competing for Rab11 binding with family-interacting proteins, and that there is a causal relationship between Rab11 ability to recruit family-interacting proteins and Rab11 redistribution. This competition reduces recycling sorting at an unclear step, resulting in clustering of single- and double-membraned vesicles. These morphological changes in Rab11 membranes are indicative of alterations in protein and lipid homeostasis during infection. Vesicular clustering creates hotspots of the vRNPs that need to interact to form an infectious particle.

Proximodistal identity during vertebrate limb regeneration is regulated by Meis homeodomain proteins

The mechanisms by which cells obtain instructions to precisely re-create the missing parts of an organ remain an unresolved question in regenerative biology. Urodele limb regeneration is a powerful model in which to study these mechanisms. Following limb amputation, blastema cells interpret the proximal-most positional identity in the stump to reproduce missing parts faithfully. Classical experiments showed the ability of retinoic acid (RA) to proximalize blastema positional values. Meis homeobox genes are involved in RA-dependent specification of proximal cell identity during limb development. To understand the molecular basis for specifying proximal positional identities during regeneration, we isolated the axolotl Meis homeobox family. Axolotl Meis genes are RA-regulated during both regeneration and embryonic limb development. During limb regeneration, Meis overexpression relocates distal blastema cells to more proximal locations, whereas Meis knockdown inhibits RA proximalization of limb blastemas. Meis genes are thus crucial targets of RA proximalizing activity on blastema cells.

Bénéfices de la diversité culturelle en entreprises : Études de cas dans les entreprises québécoises

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal

Observatory

View of Detroit Observatory from the northwest (University of Michigan). Publication information: Madison Wis. : J. J. Stoner, 1881

Newberry Hall

Cornerstone laid May 26, 1888; dedicated June 1891. Gift of Helen H. Newberry of Detroit as headquarters of Students' Christian Association. Leased by U-M as classroom in 1921; gift to U-M from SCA in 1937. Adapted as museum in 1928, named the Francis W. Kelsey Museum of Archaeology in 1953.

Newberry Hall, later Kelsey Museum

Cornerstone laid May 26, 1888; dedicated June 1891. Gift of Helen H. Newberry of Detroit as headquarters of Students' Christian Association. Leased by U-M as classroom in 1921; gift to U-M from SCA in 1937. Adapted as museum in 1928, named the Francis W. Kelsey Museum of Archaeology in 1953. Person in image.

Patrice Donovan, UM Women's Basketball, 1982

[vs U of Detroit]

Peg Harte, UM Women's Basketball, 1982

[vs U of Detroit]

Lori Gnatkowski, UM Women's Basketball, 1982

[vs U of Detroit]

Bill Dougall and Doug Bickle, UM Football, 1961

[Envelope caption: "The Talented Toe of Doug Bickle, Michigan end from Traverse City, has him tied for first place in the Big Ten. ... Bill Dougall of Detroit holds"]

Bill Dougall and Doug Bickle, UM Football, 1961

[Envelope caption: "The Talented Toe of Doug Bickle, Michigan end from Traverse City, has him tied for first place in the Big Ten. ... Bill Dougall of Detroit holds"]

Cast iron pipe /

Copy in Dept. of Rare Books and Special Collections bound in original black leather stamped in gold. P. 9-10 lacking.

Alcoa structural handbook ; a design manual for aluminum.

"Supplement; a reprint of ASCE Proceedings ...: Paper number 970, Specifications for structures of aluminum alloy 6061-T6; second progress report of the Committee of the Structural Division on Design in Lightweight Structural Alloys [and] Paper number 971, Specifications for structures of aluminum alloy 2014-T6; third progress report of the Committee of the Structural Division on Design in Lightweight Structural Alloys": 34, 32 pages at end.

Vehicle weight reduction study. Final report.

National Highway Traffic Safety Administration, Office of Research and Development, Washington, D.C.

Annual report.

Suspended publication, 1963-1983; resumed with issue for 1984-1985.