Errata du "Dictionnaire de l'Académie française" ou Remarques critiques sur les irrégularités qu'il présente : avec l'indication de certaines règles à établir ([Reprod.]) / par B. Pautex,...

Collection : Archives de la linguistique française ; 311

Supplément au "Dictionnaire complet des langues française et allemande" de l'abbé Mozin ([Reprod.]) / par A. Peschier,...

Collection : Archives de la linguistique française ; 315

Monographie du "Dictionnaire de l'Académie française" ([Reprod.]) / par Courtat

Collection : Archives de la linguistique française ; 96

Études de la langue française sur Racine ou Commentaire général et comparatif sur la diction et le style de ce grand classique... ([Reprod.]) / par M. Fontanier,...

Collection : Archives de la linguistique française ; 160

Dictionnaire critique et raisonné du langage vicieux ou réputé vicieux : ouvrage pouvant servir de complément au "Dictionnaire des difficultés de la langue française" par Laveaux ([Reprod.]) / par un ancien professeur

Collection : Archives de la linguistique française ; 318

Dictionnaire étymologique, historique et anecdotique des proverbes et des locutions proverbiales de la langue française : en rapport avec des proverbes et des locutions proverbiales des autres langues ([Reprod.]) / par P.M. Quitard

Collection : Archives de la linguistique française ; 331

Observations sur l'orthographe ou Ortografie française ; suivies d'une Histoire de la réforme orthographique : depuis le XVe siècle jusqu'à nos jours ([Reprod.]) / par Ambroise Firmin Didot...

Collection : Archives de la linguistique française ; 158

Traité de la prononciation des consonnes et des voyelles finales des mots français... ; suivi de la Prosodie de la langue française... faisant suite à "L'Art de lire à haute voix" ([Reprod.]) / par L. Dubroca

Collection : Archives de la linguistique française ; 128

Élémens de la langue française : mis à la portée des personnes qui n'ont encore étudié aucune langue par principes... ([Reprod.])

Collection : Archives de la linguistique française ; 139

Remarques sur la langue française au dix-neuvième siècle, sur le style et la composition littéraire ([Reprod.]) / par M. Francis Wey...

Collection : Archives de la linguistique française ; 405

De la prononciation française depuis le commencement du XVIe siècle, d'après les témoignages des grammairiens ([Reprod.]) / par Charles Thurot,...

Collection : Archives de la linguistique française ; 377

Le financement du logement en France / [la Documentation française] ; [réd. par] Henri Heugas-Darraspen,...

Collection : Les études de la Documentation française

Role of mutations in the phosphoprotein on RNA polymerase activity of canine distemper virus following cell culture adaptation

Abstract: The canine distemper virus A75/17 wild-type strain, which is unable to replicate in cell lines, was adapted to growth in Vero cells. Sequence comparison between the A75/17 and the Vero cell-adapted A75/17-V virus revealed 7 amino acid differences between the 2 viruses. Three of these were located in the matrix protein, three in the phosphoprotein also changing the V protein but not the C protein and one in the large protein. The phosphoprotein and the large protein constituted the viral RNA polymerase whose activity was studied by transfection experiments using a reverse genetic system with a plasmid encoding a minireplicon and expression plasmids encoding the nucleocapsid protein and the viral RNA polymerase subunits. Surprinsingly, the enzyme of A75/17 CDV was significantly more active in cell lines compared to the polymerase of A75/17-V CDV. The decrease in overall enzyme activity was found to be due to both decreased replication and transcription activity. This polymerase attenuation was confirmed in CHO cells infection stably expressing the dog SLAM receptor mainly found in dog's lymphoid organs and allowing both virus strains to enter these cells at the same efficiency. A75/17-V CDV replicated more slowly in CHODogSLAM cells than A75/17 CDV and syncytium formation was significantly decreased compared to A75/17 infected CHODogSLAM cells.. Cell culture adaptation lead to an attenuated virus strain both in vitro and in vivo with decreased polymerase activity and syncytium forming capability showing an important role of the polymerase in determining the phenoytpe of the virus. In addition, this reduced phenotype of A75/17-V CDV was shown to be due to the P mutations in the P protein only, showing an important function of the polycistronic P gene in the adaptation process. The role of the matrix protein was found not to have any effect on polymerase activity, however its participation in the adaptation process still needs to be elucidated. The accessory proteins V and C were shown to act on polymerase activity, but their functions in virus pathogenicity and in inhibiting the interferon system have not been studied in this thesis. The V proteins have an activating effect on the polymerase of both the A75/17 and the A75/17-V CDV strains. Although the C protein amino acid sequence was not changed during adaptation of wild-type canine distemper virus in Vero cells, the C protein was demonstrated to have opposite effects on polymerase activity of both virus strains suggesting a different interaction of the C protein with the proteins forming the polymerase complex, which could modulate polymeras activity. These effects were demonstrated by transfection experiments and studying recombinant viruses not expressing the C protein. Thus, the abrogation of the C protein decrease the activity of the wild-type polymerase. In contrast, the polymerase activity of the Vero cell- adapted virus is enhanced in the absence of the C protein and this has also been demonstrated with a recombinant virus, which grew faster in the first 48 hours of infection. Future studies will focus on the generation of recombinant wild-type viruses, which should be very helpful in understanding the molecular mechanisms underlying the adaptation process and the loss of pathogenicity.

Information System ProcessInnovation Adoption, Adaptation, Learning, and Unlearning: A Longitudinal Case Study

This thesis examines the history and evolution of information system process innovation (ISPI) processes (adoption, adaptation, and unlearning) within the information system development (ISD) work in an internal information system (IS) department and in two IS software house organisations in Finland over a 43-year time-period. The study offers insights into influential actors and their dependencies in deciding over ISPIs. The research usesa qualitative research approach, and the research methodology involves the description of the ISPI processes, how the actors searched for ISPIs, and how the relationships between the actors changed over time. The existing theories were evaluated using the conceptual models of the ISPI processes based on the innovationliterature in the IS area. The main focus of the study was to observe changes in the main ISPI processes over time. The main contribution of the thesis is a new theory. The term theory should be understood as 1) a new conceptual framework of the ISPI processes, 2) new ISPI concepts and categories, and the relationships between the ISPI concepts inside the ISPI processes. The study gives a comprehensive and systematic study on the history and evolution of the ISPI processes; reveals the factors that affected ISPI adoption; studies ISPI knowledge acquisition, information transfer, and adaptation mechanisms; and reveals the mechanismsaffecting ISPI unlearning; changes in the ISPI processes; and diverse actors involved in the processes. The results show that both the internal IS department and the two IS software houses sought opportunities to improve their technical skills and career paths and this created an innovative culture. When new technology generations come to the market the platform systems need to be renewed, and therefore the organisations invest in ISPIs in cycles. The extent of internal learning and experiments was higher than the external knowledge acquisition. Until the outsourcing event (1984) the decision-making was centralised and the internalIS department was very influential over ISPIs. After outsourcing, decision-making became distributed between the two IS software houses, the IS client, and itsinternal IT department. The IS client wanted to assure that information systemswould serve the business of the company and thus wanted to co-operate closely with the software organisations.