885 resultados para A-SITE CATION


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Pós-graduação em Comunicação - FAAC

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Laccases (benzendiol:oxygen oxidoreductases; EC 1.10.3.2) catalyze the oxidation of a broad range of substrates, such as polyphenols, dyes and pollutants, and thus these enzymes are widely applied in industrial, biotechnological and environmental fields. In order to improve their biotechnological applications, a deep knowledge of structural factors involved in controlling their activity, in various experimental conditions and on different substrates, is required. In the present study, a laccase from the mushroom Rigidoporus lignosus was kinetically characterized. In particular, the stability, the effects of pH, ionic strength and fluoride ion concentration on the kinetic parameters were investigated, using three di-hydroxy-benzene isomers (1,2-dihydroxy-benzene, 1,3-dihydroxy-benzene and 1,4-dihydroxy-benzene) as substrates. The catalytic constant values of the laccase showed a bell-shaped pH profile, with the same optimum pH and pK(a) values for all tested substrates. This behavior appears to be due to the presence of an ionizable residue in the enzyme active site. To identify this residue, the enzyme was derivatized with diethylpyrocarbonate to modify accessible histidine residues, which, according to structural data, are present in the active site of this enzyme. The kinetic behavior of the derivatized laccase was compared with that of the native enzyme and the derivatized residues were identified by mass spectrometry. Mass spectrometry and kinetic results suggest the main role of His-457 in the control of the catalytic activity of laccase from R. lignosus. (C) 2013 Elsevier B.V. All rights reserved.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Although many studies have shown that soil solution chemistry can be a reliable indicator of biogeochemical cycling in forest ecosystems, the effects of litter manipulations on the fluxes of dissolved elements in gravitational soil solutions have rarely been investigated. We estimated the fluxes of NH4-N, NO3-N, K, Ca, Mg, Na, Cl, dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) over the first two years after re-planting Eucalyptus trees in the coastal area of Congo. Two treatments were replicated in two blocks after clear-cutting 7-year-old stands: in treatment R, all the litter above the mineral soil was removed before planting, and in a double slash (DS) treatment, the amount of harvest residues was doubled. The soil solutions were sampled down to a depth of 4 m and the water fluxes were estimated using the Hydrus 1D model parameterized from soil moisture measurements in 4 plots. Isotopic and spectroscopic analytical techniques were used to assess the changes in dissolved organic matter (DOM) properties throughout the transfer in the soil. The first year after planting, the fluxes of NH4-N, K, Ca, Mg, Na, Cl and DOC in the topsoil of the DS treatment were 2-5 times higher than in R, which showed that litter was a major source of dissolved nutrients. Nutrient fluxes in gravitational solutions decreased sharply in the second year after planting, irrespective of the soil depth, as a result of intense nutrient uptake by Eucalyptus trees. Losses of dissolved nutrients were noticeably low in these Eucalyptus plantations despite a low cation exchange capacity, a coarse soil texture and large amounts of harvest residues left on-site at the clear cut in the DS treatment. All together, these results clarified the strong effect of litter manipulation observed on eucalypt growth in Congolese sandy soils. DOM fluxes, as well as changes in delta C-13, C:N and aromaticity of DOM throughout the soil profile showed that the organic compounds produced in the litter layer were mainly consumed by microorganisms or retained in the topsoil. Below a depth of 15 cm, most of the DOC and the DON originated from the first 2 cm of the soil and the exchanges between soil solutions and soil organic matter were low. (C) 2014 Elsevier B.V. All rights reserved.

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Despite the remarkable improvements in breast cancer (BC) characterization, accurate prediction of BC clinical behavior is often still difficult to achieve. Some studies have investigated the association between the molecular subtype, namely the basal-like BC and the pattern of relapse, however only few investigated the association between relapse pattern and immunohistochemical defined triple-negative breast cancers (TNBCs). The aim of this study was to evaluate the pattern of relapse in patients with TNBC, namely the primary distant relapse site:One-hundred twenty nine (129) invasive breast carcinomas with follow-up information were classified according to the molecular subtype using immunohistochemistry for ER, PgR and Her2. The association between TNBC and distant relapse primary site was analyzed by logistic regression. Using multivariate logistic regression analysis patients with TNBC displayed only 0.09(95% CI: 0.00-0.74; p = 0.02) the odds of, the non-TNBC patients of developing bone primary relapse. Regarding visceral and lymph-node relapse, no differences between in this cohort were found.Though classically regarded as aggressive tumors, TNBCs rarely development primary relapse in bone when compared to non-TNBC, a clinical relevant fact when investigating a metastasis of an occult or non-sampled primary BC. (C) 2014 Elsevier GmbH. All rights reserved.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The growing need for organizations to make themselves present on the Internet, the study aimed to demonstrate the importance of public relations activity in crisis management and analysis of the crisis in Americanas.com site. It also aims to analyze the electronic scene, highlighting their implications for consumer relations in e-commerce. In addition, as a consequence of conflicts, settles a loss situation, financial and reputation. A crisis begins with planning and communication failures, it is clear that public relations is essential in business today, as interest in integrating their knowledge and opinions, and maintaining a global view of the environment in which it operates. The case study analysis provided confirmation that the public relations face a growing market, which requires qualified and updated to do crisis management, especially in the online environment

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CurtaBauru (www.curtabauru.com.br) is a journalistc website which specializes on the covering of local art and culture. The idea came up from the three author's interest in Bauru's cultural scene and also from the scarcity of local media about the subject. The website was opened in the end of August 2012, and its archives include journalistic covering of some major events such as Revirada Cultural 2012, Festival Canja de Artes Integradas and Encontro de Academias de Letras e Entidades Afins. Besides that, the website is updated daily with articles about coming up events, and includes weekly sections about people and organizations related to the city's artistic scene, restaurant criticism and an agenda containing the main events of the following week

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Health education presently experiences the incorporation of information technology, thus making access to information more practical and attainable. The initiatives to make health care scientific content available virtually have shown to be useful for teaching, health care professionals and the general population. A website, however, must be constantly managed. To promote the dissemination of the website at www.educacaoemsaude.fmb.unesp.br among faculty members and undergraduate nursing students and students at Escola Técnica de Enfermagem (Vocational Nursing School) - ETE, by evaluating, among them, its graphic and functional aspects and seeking suggestions for its improvement. This is a descriptive quantitative study for which the participants were asked to fill out an instrument designed for the study. The evaluation was performed among nursing faculty members and students at Univ Estadual Paulista Júlio Mesquita Filho – UNESP and students at Escola Técnica de Enfermagem (Vocational Nursing School) – ETEC in the city of Botucatu. The number of participants totaled 88 individuals, of whom 23 were undergraduate students, 62 were students at ETEC, and 3 were faculty members. Eighty-one were females, and 7 were males. The participants’ age ranged from 17 to 56 years. Among the items that evaluated the website, the answers prevailed from “very good” to “good”. When the participants were asked what they had thought about the website, the answers were mostly positive, and the most frequently description given was “interesting”. Among the facilities, the most frequently reported was “easily understood content”, and as regards difficulties, handling the website was the most often mentioned. One hundred percent of the answers considered the maintenance of the website to be important. Suggestions for its improvement were given, and the most frequent... (Complete abstract click electronic access below)