Cloning the expression of a mammalian gene involved in the reduction of methionine sulfoxide residues in proteins.

An enzyme that reduces methionine sulfoxide [Met(O)] residues in proteins [peptide Met(O) reductase (MsrA), EC 1.8.4.6; originally identified in Escherichia coli] was purified from bovine liver, and the cDNA encoding this enzyme was cloned and sequenced. The mammalian homologue of E. coli msrA (also called pmsR) cDNA encodes a protein of 255 amino acids with a calculated molecular mass of 25,846 Da. This protein has 61% identity with the E. coli MsrA throughout a region encompassing a 199-amino acid overlap. The protein has been overexpressed in E. coli and purified to homogeneity. The mammalian recombinant MsrA can use as substrate, proteins containing Met(O) as well as other organic compounds that contain an alkyl sulfoxide group such as N-acetylMet(O), Met(O), and dimethyl sulfoxide. Northern analysis of rat tissue extracts showed that rat msrA mRNA is present in a variety of organs with the highest level found in kidney. This is consistent with the observation that kidney extracts also contained the highest level of enzyme activity.

Majmūʻah : manuscript, 1443-1473

1. Kitāb Luqṭat al-ʻajlān fī al-uṣūl / al-Zarkashī, 878 [1473] (ff. 1r-10v) -- 2. Sharḥ Qaṣīdat Gharāmī ṣaḥīḥ (ff. 11r-20v) -- 3. Manẓūmah fī al-kitābah wa-al-tajwīd ṭarīqahu / li-Muḥammad ibn Ḥasan al-Sinjārī (ff. 21r-25v) -- 4. Hādūr / Ibn Zuqqāʻah, 877 [1472-3] (ff. 26r-30v) -- 5. al-Waraqāt / Imām al-Ḥaramayn (ff. 31r-34r) -- 6. Sharḥ Yaqūlu al-ʻabd / lil-Shaykh Shihāb al-Dīn, 846 [1443] (ff. 37r-61r) -- 7. Kitāb Sharḥ al-Waraqāt / Jalāl al-Dīn al-Maḥallī (ff. 62r-75v) -- 8. An untitled incomplete work on logic (ff. 76r-127v).

Elements of Europe's energy union. Bruegel Policy Brief 2014/05, September 2014

THE ISSUE European Union energy policy is guided by three objectives: sustainability, security of supply and competitiveness. To meet its goals in these areas, the EU is updating its energy strategy with new targets for 2030. The starting point for this is the assessment of the previous EU climate and energy package, at the centre of which were the 20-20-20 targets for 2020. Although the EU is largely on track to meet these targets, EU energy policy is generally not perceived as a success. Recent events have undermined some of the assumptions on which the 2020 package was built, and the policies for achieving the 2020 targets – although at first sight effective – are far from efficient.

Challenges for the European Union in times of crisis: reactions

Since the beginning of its existence in the form of communities, this entity faced a lot of challenges that could had been stopped the European dream without the fast, prompt and appropriate reaction of the decision makers. There were a lot of difficult times in its history of more than 60 years but the ambition and need of going forward on the way of integration prevailed and today we can talk about European Union as one of the most important global players, having one of the most complex and fascinating political systems. The tenacity and the willing to succeed off the decision makers made this possible. Moments like “The Empty Chair Crisis“, changes with regards to the decision- making process, convenient for ones but inconvenient for the others, lack of consensus with regards to the new accessions, the big changes that Europe went through in the late 80s etc. showed that the decision makers can have an appropriate response whatever the problem would be and that we must stay together and go on dreaming to a united nation in the form of a federation. Nowadays we are facing maybe the most difficult moment in European Union history. Many of the member states were and still are on the edge. A lot of immediate and prompt actions were taken since the start of financial crisis, either political or economic, drove by the need of going on. We are too much into the integration process, too much dependent one of each other so that we cannot stop and simply go back only to the concept of national state.

Trends in diabetes incidence from 1992 to 2015 and projections for 2024: A Portuguese General Practitioner’s Network study

Background: Diabetes is known as a major cause of morbidity and mortality worldwide. Portugal is known as the European country with the highest prevalence of this disease. While diabetes prevalence data is updated annually in Portugal, the General Practitioner’s (GP) Sentinel Network represents the only data source on diabetes incidence. This study describes the trends in Diabetes incidence, between 1992 and 2015, and estimate projections for the future incidence rates in Portugal until 2024. Methods: An ecological time-series study was conducted using data from GP Sentinel Network between 1992 and 2015. Family doctors reported all new cases of Diabetes in their patients’ lists. Annual trends were estimated through Poisson regression models as well as the future incidence rates (until 2024), sex and age group stratified. Incidence rate projections were adjusted to the distribution of the resident Portuguese population given Statistics Portugal projections. Results: The average increase in Diabetes incidence rate was in total 4.29% (CI95% 3.80–4.80) per year under study. Until 1998–2000, the annual incidence rate was higher in women, and from 1998–2000 to 2013–2015 turn out to be higher in men. The incidence rate projected for 2022–2024 was 972.77/105 inhabitants in total, and 846.74/105 and 1114.42/105, respectively, in women and men. Conclusions: This is the first study in Portugal to estimate diabetes incidence rate projections. The disturbing reported projections seem realistic if things continue as in the past. Actually, effective public health policies will need to be undertaken to minimize this alarming future scenario.

How Climate and Vegetation Influence the fire Regime of the Alaskan Boreal Biome: The Holocene Perspective

We synthesize recent results from lake-sediment studies of Holocene fire-climate-vegetation interactions in Alaskan boreal ecosystems. At the millennial time scale, the most robust feature of these records is an increase in fire occurrence with the establishment of boreal forests dominated by Picea mariana: estimated mean fire-return intervals decreased from ≥300 yrs to as low as ∼80 yrs. This fire-vegetation relationship occurred at all sites in interior Alaska with charcoal-based fire reconstructions, regardless of the specific time of P. mariana arrival during the Holocene. The establishment of P. mariana forests was associated with a regional climatic trend toward cooler/wetter conditions. Because such climatic change should not directly enhance fire occurrence, the increase in fire frequency most likely reflects the influence of highly flammable P. mariana forests, which are more conducive to fire ignition and spread than the preceding vegetation types (tundra, and woodlands/forests dominated by Populus or Picea glauca). Increased lightning associated with altered atmospheric circulation may have also played a role in certain areas where fire frequency increased around 4000 calibrated years before present (BP) without an apparent increase in the abundance of P. mariana. When viewed together, the paleo-fire records reveal that fire histories differed among sites in the same modern fire regime and that the fire regime and plant community similar to those of today became established at different times. Thus the spatial array of regional fire regimes was non-static through the Holocene. However, the patterns and causes of the spatial variation remain largely unknown. Advancing our understanding of climate-fire-vegetation interactions in the Alaskan boreal biome will require a network of charcoal records across various ecoregions, quantitative paleoclimate reconstructions, and improved knowledge of how sedimentary charcoal records fire events.

Pliocene-Pleistocene stack of globally distributed benthic stable oxygen isotope records

We present a 5.3-Myr stack (the ''LR04'' stack) of benthic d18O records from 57 globally distributed sites aligned by an automated graphic correlation algorithm. This is the first benthic delta18O stack composed of more than three records to extend beyond 850 ka, and we use its improved signal quality to identify 24 new marine isotope stages in the early Pliocene. We also present a new LR04 age model for the Pliocene-Pleistocene derived from tuning the delta18O stack to a simple ice model based on 21 June insolation at 65 N. Stacked sedimentation rates provide additional age model constraints to prevent overtuning. Despite a conservative tuning strategy, the LR04 benthic stack exhibits significant coherency with insolation in the obliquity band throughout the entire 5.3 Myr and in the precession band for more than half of the record. The LR04 stack contains significantly more variance in benthic delta18O than previously published stacks of the late Pleistocene as the result of higher resolution records, a better alignment technique, and a greater percentage of records from the Atlantic. Finally, the relative phases of the stack's 41- and 23-kyr components suggest that the precession component of delta18O from 2.7-1.6 Ma is primarily a deep-water temperature signal and that the phase of d18O precession response changed suddenly at 1.6 Ma.

Solar radiation over and under sea ice during the POLARSTERN cruise ARK-XXVI/3 (TransArc) in summer 2011

Arctic sea ice has declined and become thinner and younger (more seasonal) during the last decade. One consequence of this is that the surface energy budget of the Arctic Ocean is changing. While the role of surface albedo has been studied intensively, it is still widely unknown how much light penetrates through sea ice into the upper ocean, affecting sea-ice mass balance, ecosystems, and geochemical processes. Here we present the first large-scale under-ice light measurements, operating spectral radiometers on a remotely operated vehicle (ROV) under Arctic sea ice in summer. This data set is used to produce an Arctic-wide map of light distribution under summer sea ice. Our results show that transmittance through first-year ice (FYI, 0.11) was almost three times larger than through multi-year ice (MYI, 0.04), and that this is mostly caused by the larger melt-pond coverage of FYI (42 vs. 23%). Also energy absorption was 50% larger in FYI than in MYI. Thus, a continuation of the observed sea-ice changes will increase the amount of light penetrating into the Arctic Ocean, enhancing sea-ice melt and affecting sea-ice and upper-ocean ecosystems.