CHINESE NITRARIA TANGUTORUM BOBR。： CHEMICAL CONSTITUENTS OF SEED OIL EXTRACTED BY SFE-CO_2

In order to develop and make good use of Nitraria tangutorum Bobr. in Qinghai-Tibetan Plateau for its ecological and medicinal values, the seed oil was extracted by SFE-CQ2 and the chemical constituents was analyzed by GC/MS. The component relative contents were determined by area nomalization. 28 components were separated from the extracts of SFE-CQj and 12 of them, which accounted for 85.99% were identified. They were(Z, Z)-9, 12-octadecadienoic acid (linoleic acid), bicyclo[ 10. 1. 0] tridec-1-ene, 7-pentadecyne, gamma-sitosterol, gamma-tocopherol, 1, -8,Z-10-hexadecatriene,9,12-octadecadienal, 24-methyl-5-cholestene-3-ol,(Z)-9,17-octadecadienal, stigmastan-3,5-dien, eicosane and so on. Among them, the relative content of (Z, Z)-9,12-octadecadienoic acid is the highest, accounting for 65.85% of the total area. It is concluded that N. tangutorum Bobr. seed oil is a rich source of linoleic acid.

Two new names in Chinese Stellaria (Caryophyllaceae)

During preparation of the account of Stellaria L (Caryophyllaceae) for the Flora of China, Volume 6, it was noticed that two names are illegitimate later homonyms: Stellaria arenaria Maximowicz, non Linnaeus, and Stellaria pilosa Franchet, non Dulac. Therefore, the following ne names (nomina nova) are provided here: Stellaria arenarioides Shi L. Chen, Rabeler & Turland and Stellaria pilosoides Shi L. Chen, Rabeler & Turland.

New method for analysis of Chinese herbal complex prescription and its application

Traditional Chinese medicine (TCM) is a great treasure of China, the analysis of which is an arduous task. The viewpoint that all chemical constituents of Chinese herbal complex prescription should be analyzed as a black box is elucidated for the first time. Intelligent multi-mode multi-column chromatographic system (IMMCC) with its hybrids is the basic method and HPLC Unified Method is the breakthrough for the black box analysis. Dang-Gui-Bu-Xue-Tang was selected as a typical TCM and a systematic separation method from non-aqueous mobile phase to pure water mobile phase was put forward in order to convert unknown sample to known sample. The a, c values and UV spectra of 66 components of Astragalus, 78 components of Angelica and 71 components of Dang-Gui-Bu-Xue-Tang were obtained. Intelligent optimization and peak identification method and software for complex samples were developed and the optimum multi-step multi-binary gradient curve of mobile phase for Astragalus was ascertained. The maximum error and minimum error of predicted retention time for all components of Astragalus are 8.62% and 0.05% respectively. All components of Astragalus were compared with those of Angelica and it is found that many components of Astragalus are the same as those of Angelica, while the contents of these components are different. Many components of Dang-Gui-Bu-Xue-Tang are also the same as those of Astragalus and Angelica with different contents.

汉语发展性阅读障碍的神经心理学研究The Neuropsychological Basic of Chinese Developmental Dyslexia by ERP Study

本论文实验结果发现汉语阅读障碍在视听觉功能上有非语言特异性的感知觉加工缺陷：他们在完成视觉任务时成绩稍差，反应时延长，ERP波形成分的峰潜伏期也延长，波幅降低，尤其是左侧枕叶视皮层对右侧视野投射信号的加工效能差，这是首次报道他们枕叶视皮质的左右差异；他们完成听觉分辨任务时，对听觉分辨的效能比正常儿童低；尤其是在听觉相关电位中的表现，峰潜伏期延长，波幅降低。他们在视听觉双通道信号整合的加工上仍表现出ERP波形成分的峰潜伏期延长，波幅较低的现象；综合这三方面的结果，可以推论阅读障碍者可能对一般的信息进行感知觉加工的效能弱。 他们在同音字判断任务中表现出有语音意识缺陷：完成同音字判断任务的正确率与正常儿童有显著的差异；对假字的加工和形似同音字的加工产生的ERP波形均与正常儿童有显著差异，这种差异尤其在左脑更明显。 结合Franck的理论，推论汉语阅读障碍形成原因：汉语阅读障碍行为表现多种多样，这些变异性可能有一些共同的神经心理基础；由于出生前神经的异常发育，遗传的异常或环境的影响而导致的大脑左右两半球功能的差异，尤其左半球引起其对语言加工的缺陷，可能伴随出现视听觉等基本的感知觉功能障碍。

Screening and analysis of bioactive compounds with biofingerprinting chromatogram analysis of traditional Chinese medicines targeting DNA by microdialysis/HPLC

Biofingerprinting chromatogram, analysis, which is defined as the comparison of fingerprinting chromatograms of the extract of traditional Chinese medicines (TCMs) before and after the interaction with biological systems (DNA, protein. cell. etc.), was proposed for screening and analysis of the multiple bioactive compounds in TCMs. A method of microdialysis sampling combined with high performance liquid chromatography (HPLC) was applied to the study of DNA-binding property for the extracts of TCMs. Seven compounds were found to bind to calf thymus DNA (ct-DNA) from the TCMs of Coptis chinensis Franch (Coptis), but only three ones from Phellodendron amurense Rupr. (Phellodendron) and none from Sophoraflavescens Ait. (Sophora) to bind to ct-DNA. respectively. Three of them were identified as berberine, palmatine and jatrorrhizine and their association constants (K) to ct-DNA were determined by microdialysis/HPLC. Competitive binding behaviors of them to ct-DNA were also investigated. © 2005 Elsevier B.V. All rights reserved.

Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights

Performance of comprehensive two-dimensional liquid chromatography system is greatly improved than we reported previously by using a silica monolithic column as for the second dimensional separation. Due to the increase of the elution speed on the second dimensional monolithic column, the first dimensional column efficiency and analysis rate can be greatly improved as comparing with conventionally second dimensional column. The developed system was applied to analysis of methanol extraction of two umbelliferae herbs Ligusticum chuanxiong Hort. and Angelica sinensis (Oliv.) Diels by using CN column as for the first dimensional separation and a silica monolithic ODS column for the second dimensional separation, and the obtained three-dimensional chromatograms were treated by normalization of peak heights with the value of the highest peak or setting a certain value using a software written in-house. It was observed that much more peaks for low-abundant components in TCM extract can clearly be detected here than we reported before, due to the large difference for the amount of components in TCMs' extract. With the above improvements in separation performance and data treatment, totally about 120 components in methanol extraction of Rhizoma chuanxiong and 100 in A. sinensis were separated with UV detection within 130 min. This result meant that both the number of peaks detected increase twice but the analysis time decease twice if comparing with the previously reported result. (c) 2005 Published by Elsevier B.V.