Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

Objective To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension. Results A multilevel factorial design was used to quantify effects of temperature (33–37 C), fresh medium addition after the viral adsorption step (100–200 % with respect to the initial cell suspension volume before infection) and harvest time (8–40 h) on RVGP production. Experimental runs were performed in 24-well cell culture plates at a multiplicity of infection (MOI) of 16. An additional experiment in spinner-flask was performed at MOI of 9, using the optimal conditions determined in cell culture plates. Values for temperature, fresh medium addition and harvest time of 33 C, 100 % and 16 h, respectively, ensured the optimal RVGP production in culture plates. The volumetric yield (239 ng ml-1 ) in these conditions was higher than that reported previously for adherent cell culture. In spinner-flasks, the volumetric yield was improved (559 ng ml-1 ). Conclusion These results establish the basis for designing bioprocess to produce RVGP.

Equações alométricas em plantios mistos visando à restauração da floresta estacional semidecidual

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

COD, TSS, nutrients and coliforms removals in UASB reactors in two stages treating swine wastewater

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Co-digestão anaeróbia de resíduos vegetais e águas residuárias de suinocultura em reatores horizontais de leito fixo e alta taxa

Pós-graduação em Microbiologia Agropecuária - FCAV

Variabilidade de atributos físicos de um latossolo vermelho sob cultivo de cana-de-açúcar

The goal of this paper was to select among the attributes surveyed soil, one with better representation to explain the variability of the technological components of sugar cane. The study was conducted at ETH Eldorado Plant in Rio Brilhante, MS, in the agricultural year 2011/2012, in a Oxisol, which was installed a geostatistical grid for data collection of soil and plant, with 80 sampling points, a 80 ha area. From the standpoint of linear and spatial TCH has been explained as a function of volumetric moisture. The volumetric moisture collected at a depth of 0.00 to 0.20 m, which had values between 0,24 to 0,270 m(3) m(-3), resulted in sites with the highest productivity of sugar cane per hectare from 64 to 70 t ha(-1). To aid future studies aimed at precision agriculture, which will use the same attributes as those of the future works, the values of ranges of spatial dependence to be used should be between 81 and 487 meters.

Physicothermal properties of aqueous sodium chloride solutions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bonding ability of paste-paste glass ionomer systems to tooth structure: in vitro studies

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Assessment of modernized GPS L5 SNR for ground-based multipath reflectometry applications

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reator anaeróbio compartimentado para o tratamento de águas residuárias de suinocultura

Water and soil pollution caused by the waste from the swine production causes severe environmental impacts in producing areas. An efficient alternative to reduce these impacts can be the use of high-rate anaerobic reactors. In this work, it was evaluated the effect of the swine wastewater with total suspended solids concentrations around 6.000 mg.L-1 in the anaerobic baffled reactor with three compartments. The volume of the first compartment was 210 L and of the second and third ones of 160 L. The anaerobic baffled reactor was submitted at hydraulic detention times of 56, 28 and 18 hours and to organic load rates of 5.0 to 10.1 g total COD (L.d)(-1). The highest total COD removals, of 42 to 68%, and volumetric methane productions, of 0.261 to 0.454 L CH4 (L reactor d)(-1), occurred in the second compartment.

Distribution of the root system of peach palm under drip irrigation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Investigation of the cingulate cortex in idiopathic generalized epilepsy

Studies using quantitative neuroimaging have shown subtle abnormalities in patients with idiopathic generalized epilepsy (IGE). These findings have several locations, but the midline parasagittal structures are most commonly implicated. The cingulate cortex is related and may be involved. The objective of the current investigation was to perform a comprehensive analysis of the cingulate cortex using multiple quantitative structural neuroimaging techniques. Thirty-two patients (18 women, 30 ± 10 years) and 36 controls (18 women, 32 ± 11 years) were imaged by 3 Tesla magnetic resonance imaging (MRI). A volumetric three-dimensional (3D) sequence was acquired and used for this investigation. Regions-of-interest were selected and voxel-based morphometry (VBM) analyses compared the cingulate cortex of the two groups using Statistical Parametric Mapping (SPM8) and VBM8 software. Cortical analyses of the cingulate gyrus was performed using Freesurfer. Images were submitted to automatic processing using built-in routines and recommendations. Structural parameters were extracted for individual analyses, and comparisons between groups were restricted to the cingulate gyrus. Finally, shape analyses was performed on the anterior rostral, anterior caudal, posterior, and isthmus cingulate using spherical harmonic description (SPHARM). VBM analyses of cingulate gyrus showed areas of gray matter atrophy, mainly in the anterior cingulate gyrus (972 mm(3) ) and the isthmus (168 mm(3) ). Individual analyses of the cingulate cortex were similar between patients with IGE and controls. Surface-based comparisons revealed abnormalities located mainly in the posterior cingulate cortex (718.12 mm(2) ). Shape analyses demonstrated a predominance of anterior and posterior cingulate abnormalities. This study suggests that patients with IGE have structural abnormalities in the cingulate gyrus mainly localized at the anterior and posterior portions. This finding is subtle and variable among patients.

Dilatometria de sólidos com resolução subatômica

The main goal of this bachelor work is the study of the physical properties of solids by thermal expansion coefficient measurements, which allows the exploration of various aspects of phase transitions. In this study, some theoretical aspects were focused, for example the first and second order phase transitions, Landau theory for phase transition and the volumetric thermal expansion coefficient. Furthermore, on the origin of the thermal expansion from the Lennard-Jones potential, that contains the anharmonic terms, which give rise to the phononic thermal expansion in solids

Dinâmica populacional e manejo de leptocybe invasa (Hymenoptera: Eulophidae) em plantações de eucalipto

Pós-graduação em Ciência Florestal - FCA

Distribuição espacial da deriva de produtos fitossanitários em pulverizações na cultura da laranja

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Contração de polimerização e expansão higroscópica de resinas compostas: análise pela técnica de video-imagem

To evaluate the volumetric changes due to polymerization and thermocycling on different resin-composites. Methods: Thirteen A2 Universal Dentin shade resin-composites (n = 10) from eight manufacturers were evaluated (4Seasons, Grandio, Venus, Amelogen Plus, P90, Z350, Esthet-X, Amaris, Vita-l-escence, Natural-Look, Charisma, Z250 and Opallis). The polymerization shrinkage percentage (PS) was calculated using an image measurement device (ACUVOL - Bisco Dental). Equal volumes of material, standardized by a semisphere polyurethane matrix (d = 3mm) were used and, after 5 minutes of relaxation, the baseline volume measurements were obtained with 18 J of energy dose from the LED light-curing unit. Measurements were obtained after 5 minutes and PS values calculated. Specimens were stored in a drydark environment for 24 hours and re-measured. Specimens were then thermocycled in distilled water between 5oC and 55oC for 20,000 cycles, subjected to another volume measurement at 5,000 cycle intervals. Specimens were gently dried prior to each measurement. Results: Repeated measurements were made using ANOVA (α = 0.05) showed that all resin-composite volumes were influenced by the number of cycles. Volumes at 5 minutes post-polymerization (12.47 ± 0.08) were significantly lower than those at baseline (12.80 ± 0.09). Volumes at 24 hours (12.43 ± 0.19) were insignificantly lower than those at 5 minutes postpolymerization. With regards to the impact of thermocycling, all specimens showed statistically significant increases in volume after 5,000 cycles (13.04 ± 0.22). Although statistically different from those after 5,000 cycles, there was no statistically significant difference between volumes measured at 10,000 (12.87±0.21), 15,000 (12.92±0.24), and 20,000 (12.84±0.23) cycles. Conclusion: According to the video-imaging analysis, thermocycling caused a significant expansion in resin-composites tested, the volume increase was not able to...