A habitat-use model to determine essential fish habitat for juvenile brown shrimp (Farfantepenaeus aztecus) in Galveston Bay, Texas

A density prediction model for juvenile brown shrimp (Farfantepenaeus aztecus) was developed by using three bottom types, five salinity zones, and four seasons to quantify patterns of habitat use in Galveston Bay, Texas. Sixteen years of quantitative density data were used. Bottom types were vegetated marsh edge, submerged aquatic vegetation, and shallow nonvegetated bottom. Multiple regression was used to develop density estimates, and the resultant formula was then coupled with a geographical information system (GIS) to provide a spatial mosaic (map) of predicted habitat use. Results indicated that juvenile brown shrimp (<100 mm) selected vegetated habitats in salinities of 15−25 ppt and that seagrasses were selected over marsh edge where they co-occurred. Our results provide a spatially resolved estimate of high-density areas that will help designate essential fish habitat (EFH) in Galveston Bay. In addition, using this modeling technique, we were able to provide an estimate of the overall population of juvenile brown shrimp (<100 mm) in shallow water habitats within the bay of approximately 1.3 billion. Furthermore, the geographic range of the model was assessed by plotting observed (actual) versus expected (model) brown shrimp densities in three other Texas bays. Similar habitat-use patterns were observed in all three bays—each having a coefficient of determination >0.50. These results indicate that this model may have a broader geographic application and is a plausible approach in refining current EFH designations for all Gulf of Mexico estuaries with similar geomorphological and hydrological characteristics.

The physiological effects of multiple forced submergences in loggerhead sea turtles (Caretta caretta)

Sea turtles are subjected to involuntary submergence and potential mortality due to incidental capture by the commercial shrimp fishing industry. Despite implementation of turtle excluder devices (TEDs) to reduce at-sea mortality, dead stranded turtles continue to be found in near-record numbers along the coasts of the western Atlantic Ocean and northern Gulf of Mexico. Although this mortality may be due to an increase in the number of turtles available to strand, one alternative explanation is that sea turtles are repetitively submerged (as one fishing vessel follows the path of another) in legal TEDs. In the present study, laboratory and field investigations were undertaken to examine the physiological effects of multiple submergence of loggerhead sea turtles (Caretta caretta). Turtles in the laboratory study were confined during the submersion episodes, whereas under field conditions, turtles were released directly into TED-equipped commercial fishing nets. Under laboratory and field conditions, pre- and postsubmergence blood samples were collected from turtles submerged three times at 7.5 min per episode with an in-water rest interval of 10, 42, or 180 min between submergences. Analyses of pre- and postsubmergence blood samples revealed that the initial submergence produced a severe and pronounced metabolic and respiratory acidosis in all turtles. Successive submergences produced significant changes in blood pH, Pco2, and lactate, although the magnitude of the acid-base imbalance was substantially reduced as the number of submergences increased. In addition, increasing the interval between successive submergences permitted greater recovery of blood homeostasis. No turtles died during these studies. Taken together, these data suggest that repetitive sub-mergence of sea turtles in TEDs would not significantly affect their survival potential provided that the animal has an adequate rest interval at the surface between successive submergences.

Catch assessment of indigenous and exotic carp species of Nasti baor

An investigation on length-weight relationship, length-frequency distribution, catch per unit of effort (CPUE) and stocking and harvesting status of three Indian major carps: rohu Labeo rohita, catla Catla catla and mrigal Cirrhinus mrigala and three exotic carps: silver carp Hypophthalmichthys molitrix, grass carp Ctenopharyngodon idella) and common carp Cyprinus carpio was carried out in Nasti baor (oxbow lake) for the harvesting season from August to December 1995. The length-weight relationship for six carp species was established for the harvesting months of November and December 1995. The b values for different species respectively for the months of November and December were 2.95 and 2.58 for rohu, 3.06 and 2.98 for catla, 2,84 and 2.90 for mrigal, 2.75 and 2.60 for silver carp, 2.51 and 1.97 for grass carp and 2.38 and 2.50 for common carp. In CPUE study, the CPUE was 0.58 kg/ha/hr while the catch per gear was 0.08 kg/ha/hr/purse-seine. The recovery percentage of mrigal was highest (63.57%) and it was lowest (16.81%) in case of silver carp. The density of submerged macrophytes (Hydrilla, Utricularia, Ceratophyllum and Vallisneria) was highest (4.39 kg/sqm) in November and was lowest (0.76 kg/sqm) in September.

重组人甲状旁腺素(rhPTH(1-84))的原核表达及发酵条件初步优化

下载PDF阅读器甲状旁腺激素(Parathyroid Hormone,PTH)是治疗骨质疏松症的药物之一.将人工合成全长人PTH(hPTH(1-84))的核苷酸序列插入pThioHis A载体中,然后转化大肠杆菌(Escherichia coli.),在IPTG的诱导下,成功实现了rhPTH(1-84)的原核表达.通过发酵条件的优化,初步确定1:40接种.LB+30% M9盐溶液的发酵培养基,37℃培养至OD600nm=0.8时,加入终浓度为0.6 mmol/L的IPTG,诱导8 h的较优发酵程序.

A report on frame and catch assessment surveys on Lake Nakuwa, Lake Kyoga Basin, Uganda

Lake Nakuwa is one of the large lakes among the Kyoga drainage system lakes, located 132 km north east Off Jinja town, at 01° 091N 33° 21 1 E, an elevation 1037 m, surface area of 200 km2 and an average depth of 3.3 m. The lake is shared by the districts of Kamuli, Pallisa and the newly created district of Kaliro. howerever 80% of the landing sites are in Kaliro and less than 20% are shared between the districts of Kamuli and Pallisa. The lake is free of submerged and floating macrophytes, with lots of floating papyrus (sudds). Papyrus, hippo grass and reeds dominate the shoreline vegetation. Lake Nakuwa like the main lake Kyoga was stocked with the Nile perch and the tilapiine species namely Oreochromis niloticus, Oreochromis leucostictus and Tilapia zillii in the general stocking exercise of small lakes alild dams in the early 1970's.

Evaluation of biochemical characteristics of a traditional salt fermented fish product of northeast India with special reference to its flavour components

'Lona ilish', is a traditional salt fermented fish product, widely consumed and very popular in north-east part of India and Bangladesh. It is prepared exclusively from a high fat fish, Hilsa (Tenualosa) ilisha. 'Lona ilish' was prepared in the laboratory following traditional process. After 150 days of fermentation, a better quality 'lona ilish' was obtained. Biochemical characteristics of market sample was estimated and compared with the laboratory prepared one. A variation in biochemical composition was observed. Sensory quality of the final product of laboratory prepared 'lona ilish' was compared with the market sample and found that the laboratory prepared product scored better than the market sample. The moisture (49.89%) and salt (15.48%) of the final product was found to be satisfactory for stability of the 'lona ilish' at ambient temperature. Analysis of volatile compounds of 'lona ilish' was done using GC-MS. It was concluded that, aldehydes, ketones and esters may possibly contribute characteristic aromas to the overall flavour of the salt fermented hilsa.

Durability study of eleven years old cement-bentonite cut-off wall material

Comprehensive understanding of the long-term performance of cement-bentonite slurry trench cut-off walls is essential as these mixes may degrade when exposed to aggressive environments or when subjected to prolonged drying. A series of wetting-drying and immersion experiments was carried out to evaluate the durability characteristics of laboratory mixed samples and block field samples from 40 days to 11 years of age. For the wetting-drying tests, the samples buried in medium graded sand were subjected to periodical flooding and drying cycles. They were then used for permeability testing and unconfined compressive strength (UCS) testing. For the immersion tests, the samples confined in perforated molds were submerged in magnesium sulfate solution for 16 weeks and their microstructures were then analyzed using X-ray diffraction (XRD) technique. This paper identifies the effects of contaminant exposure on durability of cement-bentonite and the effects of aging by comparing 11 years old samples to younger samples. Test results showed that young or previously contaminated cement-bentonite mixes are more susceptible to sulfate attack than old or less contaminated mixes. Copyright ASCE 2008.

Ecology and settlement of marine fouling in the Suez Bay, Egypt

This study deals with seasonal variations, natural correlations and similarities of fouling assemblages on exposure panels in the Suez Bay during January 1992 to January 1993. Three main sources of pollutions flow into the bay; industrial waste products, domestic drainage of Suez city and ships' oil and refuse.The fouling assemblages on the test pan els after various periods (1, 2 and 3 months) belonged mainly to the algae (Ulva rigida), polychaetes (Hydroides elegans), Cirripedes (Balanus amphitrite) and amphipods. The fouling at the lst station was relatively more dense than at the 2nd station during the summer and autumn seasons. The lowest productivity was achieved at the 3rd station which was considered less polluted being offshore water. The overall paucity of fouling in the bay is because of the silt covering the submerged surfaces, particularly at the 2nd station, leading to the prevention of the settlements or establishment of fouling organisms. The seasonal changes in the intensity of fouling assemblages on submerged surfaces in seawater seems to be closely related to seasonal variations in water temperature. The great fouling communities on the buoys and long exposure panels showed a remarkable variety of species and density rather than on short term exposures, which were more dense during warmer months.

Growth performance and nutrient utilization of catfish Clarias gariepinus fed varying inclusion level of fermented unsieved yellow maize

This study was conducted using 150 fish of Clarias gariepinus to investigate the growth performance and nutrient utilization of Clarias gariepinus fed five treatment diets containing varying inclusion level of fermented unsieved maize. The diets were grouped into CT, T1, T2, T3, and T4 with inclusion levels of 25%, 50%, 75%, and 100% of fermented unsieved maize respectively. Highest weight gain was recorded in T4 with value of 10.24 and lowest weight was recorded in CT with 9.17. High FCR were observed in T2 with value of 0.70 and lower value was observed in T4 with value of 0.62. While, T2, T3, and T4 have highest survival rates with values of 90% in each treatment CT and T1 recorded 80% and 70% respectively. There was a significant (p< 0.05) difference between the food conversion ratios treatment T4 with the best value and other treatments. There was a significant (p< 0.05) difference between the levels of fermented unsieved maize inclusion and the specific growth rate of the experimental fish. The highest value of protein level and feed efficiency were observed in T4 at significant difference level (p< 0.05) than other treatments. It was concluded that fermentation of maize in fish feed has positive effects on the nutritional value of the feed. It is recommend that fermented maize can replace raw maize in fish feed diet for growth performance. KEYWORDS: Fermentation, yellow maize, Clarias gariepinus, Fish, Feed.

Growth performance and nutrient utilization of catfish Clarias gariepinus fed varying inclusion level of fermented unsieved yellow maize

This study was conducted using 150 fish of Clarias gariepinus to investigate the growth performance and nutrient utilization of Clarias gariepinus fed five treatment diets containing varying inclusion level of fermented unsieved maize. The diets were grouped into CT, T1, T2, T3, and T4 with inclusion levels of 25%, 50%, 75%, and 100% of fermented unsieved maize respectively. Highest weight gain was recorded in T4 with value of 10.24 and lowest weight was recorded in CT with 9.17. High FCR were observed in T2 with value of 0.70 and lower value was observed in T4 with value of 0.62. While, T2, T3, and T4 have highest survival rates with values of 90% in each treatment CT and T1 recorded 80% and 70% respectively. There was a significant (p< 0.05) difference between the food conversion ratios treatment T4 with the best value and other treatments. There was a significant (p< 0.05) difference between the levels of fermented unsieved maize inclusion and the specific growth rate of the experimental fish. The highest value of protein level and feed efficiency were observed in T4 at significant difference level (p< 0.05) than other treatments. It was concluded that fermentation of maize in fish feed has positive effects on the nutritional value of the feed. It is recommend that fermented maize can replace raw maize in fish feed diet for growth performance.

Assessment of nutritional quality of 'Shidal' a fermented fish product of northeast India

Shidal is a salt-free fermented fish product prepared from Puntius ss. caught in late monsoon period. Shidal is very popular amongst the inhabitants of Northeast India. The fermentation process of this product takes four to six months in anaerobic condition till the product gains a characteristic flavour and colour. Detailed studies on the biochemical and nutritive qualities of this product are very few. Therefore, in this paper we report the results of the proximate analysis, amino acid and fatty acid compositions. The results suggest that Shidal is a rich source of amino acids as well as of essential fatty acids like linoleic and linolenic acids. The n- 3/n-6 ratio was found 0.51.

Simplifying mooring analysis for deepwater systems using truncation

Over recent years academia and industry have engaged with the challenge of model testing deepwater structures at conventional scales. One approach to the limited depth problem has been to truncate the lines. This concept will be introduced, highlighting the need to better understand line dynamic processes. The type of line truncation developed here models the upper sections of each line in detail, capturing wave action and all coupling effects with the vessel, terminating to an approximate analytical model that aims to simulate the remainder of the line. A rationale for this is that in deep water transverse elastic waves of a line are likely to decay before they are reflected at the seabed because of nonlinear hydrodynamic drag forces. The first part of this paper is centered on verification of this rationale. A simplified model of a mooring line that describes the transverse dynamics in wave frequency is used, adopting the equation of motion of an inextensible taut string. The line is submerged in still water, one end fixed at the bottom the other assumed to follow the vessel response, which can be harmonic or random. A dimensional analysis, supported by exact benchmark numerical solutions, has shown that it is possible to produce a universal curve for the decay of transverse vibrations along the line, which is suitable for any kind of line with any top motion. This has a significant engineering benefit, allowing for a rapid assessment of line dynamics - it can be useful in deciding whether a truncated line model is appropriate, and if so, at which point truncation might be applied. This is followed by developing a truncation mechanism, formulating an end approximation that can reproduce the correct impedance, had the line been continuous to full depth. It has been found that below a certain length criterion, which is also universal, the transverse vibrational characteristics for each line are inertia driven. As such the truncated model can assume a linear damper whose coefficient depends on the line properties and frequency of vibration. Copyright © 2011 by the International Society of Offshore and Polar Engineers (ISOPE).

Active control of sound radiated by a submarine hull in bending vibration using inertial actuators

Submarines are efficient sources of low frequency radiated noise due to the vibrations induced by the rotation of the propeller in a non uniform wake. In this work the possibility of using inertial actuators to reduce the far field sound pressure is investigated. The submerged vessel is modelled as a cylindrical shell with two conical end caps. Complicating effects such as ring stiffeners, bulkheads and the fluid loading are taken into account. A harmonic radial force is transmitted from the propeller to the hull through the stern end cone and it is tonal at the blade passing frequency (rotational speed of the shaft multiplied by the number of blades). The actuators are attached at the inside of the prow end cone to form a circumferential array. Both Active Vibration Control (AVC) and Active Structural Acoustic Control (ASAC) are analysed and it is shown that the inertial actuators can significantly reduce the far field sound pressure.

Pilot plant for production of fish ensilage and the economics of production

This communication describes the design aspect and functions of individual pieces of equipment of a pilot plant for the production of fish ensilage based on lactic acid fermentation process. Details about the equipment, process flow sheet and equipment layout of the pilot plant have been given. An attempt has been made to prepare an estimate of the cost of production of liquid ensilage and solid feed mix.

Duplication and functional diversification of pancreatic ribonuclease (RNASE1) gene

Adaptation is one of the most fundamental issues in the studies of organismal evolution. Pancreatic ribonuclease is a very important digestive enzyme and secreted by the pancreas. Numerous studies have suggested that RNASE1 gene duplication is closely related to the functional adaptation of the digestive system in the intestinal fermentation herbivores. RNASE1 gene thus becomes one of the most important candidate genetic markers to study the molecular mechanism of adaptation of organisms to the feeding habit. Interestingly, RNASE1 gene duplication has also been found in some non-intestinal fermentation mammals, suggesting that RNASE1 gene may have produced novel tissue specificity or functions in these species. In this review, RNASE1 gene and its implications in adaptive evolution, especially in association with the feeding habit of organisms, are summarized.