Lottery Action, May 3-16, 2004, Vol. 11, no. 9

Iowa Lottery Authority Retailer Information Newsletter

Lottery Action, June 7-20, 2004, Vol. 11, no. 11

Iowa Lottery Authority Retailer Information Newsletter

Lottery Action, September 22-October 5, 2003, Vol. 10, no. 19

Iowa Lottery Authority Retailer Information Newsletter

Lottery Action, October 20 - November 2, 2003, Vol. 10, no. 21

Iowa Lottery Authority Retailer Information Newsletter

Lottery Action, December 15, 2003 - January 4, 2004, Vol. 10, no. 25

Iowa Lottery Authority Retailer Information Newsletter

Iowa Department of Transportation Strategic Plan, January 2003 Through December 2006, June 2004

To provide safe and modern transportation systems and services to individuals who travel in Iowa, the Iowa Department of Transportation has identified three broad-based and far-reaching strategic goals: accessibility, responsiveness and accountability. These goals are outlined in this plan, which is aligned with the Accountable Government Act. In addition, the plan specifically addresses strategic needs to serve our customers, and to respond to trends of a growing population and the ever changing need to enhance and expand Iowa's transportation systems. This plan is a living document, and therefore, will be updated periodically.

Iowa Commission on the Status of African-Americans, Strategic Plan, 2004

Strategic Plan for the Iowa Commission on the Status of African-Americans

Lottery Action, July 12-25, 2004, Vol. 11, no. 13

Iowa Lottery Authority Retailer Information Newsletter

Community Action In Iowa Annual Report, 2000

Iowa Division of Community Action Agencies Annual Report

Community Action In Iowa Annual Report, 2001

Iowa Division of Community Action Agencies Annual Report

Community Action In Iowa Annual Report, 2002

Iowa Division of Community Action Agencies Annual Report

Community Action In Iowa Annual Report, 1999

Iowa Division of Community Action Agencies Annual Report

Lymphodepletion by short-term chemotherapy does not alter the highly stable and persistent EBV specific CD8 T cell repertoire

Protective T cell responses againstpersistent viruses like Epstein-Barrvirus in healthy individuals are characterizedby a remarkable stability ofthe T cell receptor (TCR) clonotypicrepertoire, with highly preservedclonotype selection and persistenceover time. Here, we extended recentwork to the study of EBV-specificCD8 T cell responses in melanomapatients treated by short-term chemotherapyfor transient lymphodepletion,followed by adoptive cell transfer(ACT) and immune reconstitutionfor cancer therapy. After this treatment,we observed increased proportionsof virus-specific T cells in 3/5patients, accompanied by a more differentiatedphenotype (EMRA/CD28neg), compared to specific cells ofhealthy individuals. Yet, similarly tohealthy donors, clonotype selectionand composition of virus-specific Tcells varied along the pathway of celldifferentiation, with some clonotypesthat were selected with late differentiation,while others were not. Aftertreatment, we did not observe noveldominant clonotypes, likely related toabsence of EBV reactivation measuredas viral load levels by quantitativePCR in PBMCs and antibody levelsin plasma samples. Furthermore,public TCR BV signatures were frequentlyfound within T cell clonotypesthat dominated the repertoiresof patients, in line with those observedin healthy individuals. Ourfindings indicate that even in situationswhere the immune system isstrongly challenged such as followinglymphodepletion and homeostatic repopulation,cytotoxic T cells specificfor EBV remain strikingly stable interms of clonotype selection and com-position along T cell differentiation.We are currently characterizing theclonotype selection and gene expressionprofiles of single EBV-specificCD8 T lymphocytes sorted ex-vivo inone patient who underwent two cyclesof lymphodepletion with escaladingdoses of chemotherapy overone-year interval. Observations madefrom this setting will provide additionalinsight into the degree of stabilityof virus specific T cells, and changesin the expression levels of genesimportant for cytolytic function andlong-term survival of T cells. Thiswork is supported by the Swiss NationalCenter of Competence in Research(NCCR) Molecular Oncology,and the Swiss National Science Foundation.

Lottery Action, July 26 - August 8, 2004, Vol. 11, no. 14

Iowa Lottery Authority Retailer Information Newsletter