Seawater carbonate chemistry and brown dottyback (Pseudochromis fuscus) movement and feeding behaviour during experiments, 2011

Changes in olfactory-mediated behaviour caused by elevated CO2 levels in the ocean could affect recruitment to reef fish populations because larval fish become more vulnerable to predation. However, it is currently unclear how elevated CO2 will impact the other key part of the predator-prey interaction - the predators. We investigated the effects of elevated CO2 and reduced pH on olfactory preferences, activity levels and feeding behaviour of a common coral reef meso-predator, the brown dottyback (Pseudochromis fuscus). Predators were exposed to either current-day CO2 levels or one of two elevated CO2 levels (~600 µatm or ~950 µatm) that may occur by 2100 according to climate change predictions. Exposure to elevated CO2 and reduced pH caused a shift from preference to avoidance of the smell of injured prey, with CO2treated predators spending approximately 20% less time in a water stream containing prey odour compared with controls. Furthermore, activity levels of fish was higher in the high CO2 treatment and feeding activity was lower for fish in the mid CO2treatment; indicating that future conditions may potentially reduce the ability of the fish to respond rapidly to fluctuations in food availability. Elevated activity levels of predators in the high CO2 treatment, however, may compensate for reduced olfactory ability, as greater movement facilitated visual detection of food. Our findings show that, at least for the species tested to date, both parties in the predator-prey relationship may be affected by ocean acidification. Although impairment of olfactory-mediated behaviour of predators might reduce the risk of predation for larval fishes, the magnitude of the observed effects of elevated CO2 acidification appear to be more dramatic for prey compared to predators. Thus, it is unlikely that the altered behaviour of predators is sufficient to fully compensate for the effects of ocean acidification on prey mortality.

Predator nonconsumptive effects on prey recruitment weaken with recruit density, supplementary material

We investigated the nonconsumptive effects (NCEs) of predatory dogwhelks (Nucella lapillus) on intertidal barnacle (Semibalanus balanoides) recruitment through field experiments on the Gulf of St. Lawrence coast and the Atlantic coast of Nova Scotia, Canada. We studied the recruitment seasons (May-June) of 2011 and 2013. In 2011, the Gulf coast had five times more nearshore phytoplankton (food for barnacle larvae and recruits) during the recruitment season and yielded a 58% higher barnacle recruit density than the Atlantic coast at the end of the recruitment season. In 2013, phytoplankton levels and barnacle recruit density were similar on both coasts and also lower than for the Gulf coast in 2011. Using the comparative-experimental method, the manipulation of dogwhelk presence (without allowing physical contact with prey) revealed that dogwhelk cues limited barnacle recruitment under moderate recruit densities (Atlantic 2011/2013 and Gulf 2013) but had no effect under a high recruit density (Gulf 2011). Barnacle recruits attract settling larvae through chemical cues. Thus, the highest recruit density appears to have neutralized dogwhelk effects. This study suggests that the predation risk perceived by settling larvae may decrease with increasing recruit density and that prey food supply may indirectly influence predator NCEs on prey recruitment.

The present status of the fishery for tilapia in Lake Victoria (Uganda)

The commercial fisheries of Lake Victoria are presently dominated by three species: the stocked Lates niloticus and Oreochromis niloticus, and the endemic cyprinid Rastrineobola argentea. The three comprise at least 90% of the commercial catch while the rest of the endemic species mostly occur as by-catch (incidental catch) except in localised areas. Apart from being a major source of food, the three species especially the Nile perch represent the usually recognized main forms of predation, As they exert a "top-down" effect on production, they are important in the trophic dynamics of the Lake Victoria ecosystem. However, another form of predation which is usually unrecognized in the lake productivity mechanisms is one due to fishing mortality. Fishermen essentially behave as predatory elements in the ecosystem. This is manifested in ways that paral1el the effect of fish as predators e.g. some fishermen are habitat restricted and specialised in catching particular species or sizes, others are opportunistic and switch to whatever species (prey) are available which may depend on season, etc. There are also indirect factors that influence fishing mortality as a form of predation e.g. availability on the market of different gears, thefts of nets and of fish from nets, civil strife, market demand etc. The fatter are essentially socioeconomic factors. Application of the principles of fisheries management requires "a data base from which effective options can be generated. It is considered that one of the fundamental requirements for such a data base is information on the spatial distribution of the species fishery. This can be combined with information on landings which can eventually be incorporated into a programme of stock monitoring. The aim of this paper is to highlight information on the Tilapia fishery that may benefit fisheries management.

Toxicidade de extratos vegetais ao percevejo bronzeado do eucalipto Thaumastocoris peregrinus (hemiptera: heteroptera: thaumastocoridae) e organismos não-alvo

Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae ) is an insect from Australia which is causing severe damage to eucalyptus crops around the world. When feeding from the leaves sap, it causes bronzening, and in extreme cases, may lead to the tree death. Control methods have been studied and the most promising so far is the egg parasitoid Cleruchoides noackae (Hymenoptera: Mymaridae). Alternative products from plants with insecticidal properties could also be a viable option, and they might even be used concomitantly with C. noackae, aiming for a most effective control, but still safe for the environment. Thus, the objective of this work was to verify the action of 5% aqueous plant extracts of Matricaria chamomilla, Echinodorus grandiflorus, Punica granatum, Maytenus ilicifolia a n d Origanum majorana on T. peregrinus. In addition, we aimed to study the extracts potential toxicity to C. noackae and Gallus domesticus L., since the plant compounds might have negative effect upon the non-target organisms. At first, HPLC (High Performance Liquid Chromatography) was used to verify which phenolic compounds would be found in the plant extracts. These were tested on bronze bug adults, in confinement test (to verify the insecticidal action of the extracts) and free-choice test (to verify the repellency). The extracts that showed better results were selected for further tests with non-target organisms. Regarding C. noackae, pre-parasitism and post-parasitism, confinement and free-choice tests were performed to verify if the extracts would affect the host-choosing by the female or the development of the immature stages of the parasitoid. To verify if the extracts would be toxic to G. domesticus, the plant extracts were added to young birds feed for five days. Parameters such as weight gain, food intake, quantification of serum enzymes and histopathological analysis were carried out. HPLC analysis detected gallic, ferulic, vanillic, caffeic and cumaric acid in the extracts samples. All plant extracts tested reduced T. peregrinus survival, but E. grandiflorus, Matricaria chamomilla Maytenus ilicifolia had also a repellent effect, and were tested on the non-target organisms. None of these extracts affected neither the host choice by C. noackae nor adult emergency, when compared to the control group. In addition, the extracts did not cause alterations in any of the studied parameters. Thus, we verified that E. grandiflorus, Matricaria chamomilla and Maytenus ilicifolia have potential to be used to control T. peregrinus and are safe to C. noackae and G. domesticus.

Población anidante e impacto en la pesca artesanal del cormorán neotropical Phalacrocorax brasilianus (Suliformes:Phalacrocoracidae), en el sitio Ramsar Cerrón Grande, El Salvador.

El cormorán neotropical (Phalacrocorax brasilianus) es una especie de ave acuática abundante en los humedales de El Salvador. La depredación de peces que realiza en los ecosistemas acuáticos, está generando un conflicto con los pescadores que asocian la disminución de la pesca con la conducta depredadora del ave. Entre abril de 2010 a febrero de 2011, se realizó una investigación tendiente a conocer el estado de la población de P. brasilianus en el Sitio Ramsar Embalse Cerrón Grande, El Salvador. El estudio se desarrolló por medio de cinco censos y recolecta de ejemplares, estos últimos se utilizaron para analizar el contenido estomacal. El máximo de ejemplares contabilizados fue de 28,063 individuos y 3,000 parejas reproductoras. Se recolectaron 266 ejemplares, de los cuales, 207 contenían peces en sus estómagos,cuyo peso individual varío de 2 a 307 g ±52 g.Se encontró un total de 1,078 ejemplares de peces correspondiente a diez especies, cuatro de ellas obtuvieron los mayores valores de abundancia: bagre (Cathorops steindachneri)con 29%, seguido por guapote (Parachromis managuensis) con 25%, tilapia (Oreochromis niloticus) con 22% y plateada (Astianax aeneus, Roeboides bouchellei) con 21%. ABSTRACT: The neotropical cormorant (Phalacrocorax brasilianus) is a species of waterfowl abundant in the wetlands of El Salvador. Fish predation on aquatic ecosystems is generating a conflict with fishermen that associating declining fishing with predatory behavior of this bird. From April 2010 to February 2011, an investigation was conducted aimed at knowing the state of the population of P. brasilianus in the Ramsar wetland Cerron Grande, El Salvador. The study was conducted through counting and collecting of birds, to analyze the stomachic contents. The maximum recorded was 28.063 individuals and 3,000 breeding pairs. 266 birds were collected, on 207 it found fish in their stomachs, whose individual weight ranged from 2-307 g ± 52 g. A total of 1,078 fish specimens was found, from ten species, four of them obtained the highest values of abundance: bagre (Cathorops steindachneri) with 29%, followed by guapote (Parachromis managuensis) with 25%,tilapia (Oreochromis niloticus) with 22% and plateada (Astyanax aeneus, Roeboides bouchellei) with 31%.

Optimizing Western Flower Thrips Management on French Beans by Combined Use of Beneficials and Imidacloprid

Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is an important pest of vegetable crops worldwide and has developed resistance to many insecticides. The predatory mites Neoseiulus (=Amblyseius) cucumeris (Oudemans), the entomopathogenic fungus Metarhizium anisopliae (Metsch.), and an insecticide (imidacloprid) were tested for their efficacy to reduce WFT population density and damage to French bean (Phaseolus vulgaris L.) pods under field conditions in two planting periods. Metarhizium anisopliae was applied as a foliar spray weekly at a rate of one litre spray volume per plot while imidacloprid was applied as a soil drench every two weeks at a rate of two litres of a mixture of water and imidacloprid per m(2). Neoseiulus cucumeris was released every two weeks on plant foliage at a rate of three mites per plant. Single and combined treatment applications reduced WFT population density by at least three times and WFT damage to French bean pods by at least 1.7 times compared with untreated plots. The benefit-cost ratios in management of WFT were profitable with highest returns realized on imidacloprid treated plots. The results indicate that M. anisopliae, N. cucumeris, and imidacloprid have the potential for use in developing an integrated pest management program against WFT on French beans.

Social buffering of fear in zebrafish

Tese de Doutoramento em Biologia Comportamental apresentada ao ISPA - Instituto Universitário

Arthropods associated with the crown of Mauritia flexuosa (Arecaceae) palm trees in three different environments from Brazilian Cerrado

Artrópodos associados à copa de árvores, principalmente palmeiras, são pouco conhecidos no Cerrado. Para descrever a estrutura da comunidade de artrópodos à copa de Mauritia flexuosa (Arecaceae) foram amostradas 150 palmeiras em seis veredas” do Distrito Federal, de áreas silvestres, rurais e periurbanas na estação chuvosa. Os artrópodos presentes nos ninhos abandonados de aves, refúgios de mamíferos, folhas e matéria orgânica foram coletados manualmente, fixados em etanol 70% e separados em ordem, família, morfoespécie e guildas alimentares. As características das palmeiras medidas foram altura da estipe, diâmetro da copa, número de folhas e de ninhos de aves nas palmeiras. Foram coletados 3.862 indivíduos, pertencentes a 15 ordens, 45 famílias e 135 morfoespécies. As ordens mais abundantes foram Coleoptera (28,6%), Blattodea (21,8%), Collembola (11,4%) e Hemiptera (10,2%). As famílias Blaberidae, Tenebrionidae, Entomobryidae, Reduviidae, Oniscidae, Staphylinidae, Carabidae e Formicidae representaram 82,1% de todos os indivíduos coletados. A maioria das morfoespécies foi pouco abundante, 71 (52,6%) apresentaram uma abundância média igual ou menor que 1 indivíduo/palmeira. Coleoptera compreendeu o maior número de morfoespécies (43,7%) seguida de Araneae (20,0%). A análise das guildas alimentares mostrou prevalência de predadores e hematófagos (36,0%). A riqueza e a abundância de artrópodos foram menores no ambiente periurbano. O número de ninhos de aves apresentou correlação positiva com abundância e riqueza, o que não ocorreu com as medidas das palmeiras. A importância de M. flexuosa para a manutenção da artropodofauna nas “veredas” no bioma Cerrado é discutida. _______________________________________________________________________________________ ABSTRACT

Domestic Rhodnius ecuadoriensis (Hemiptera, Reduviidae) infestation in Northern Peru: a comparative trial of detection methods during a six-month follow-up

Two passive methods in the assessment of intradomiciliary infestation by Rhodnius ecuadoriensis were tested: (i) the Gomes Nuñez sensor box (GN), (ii) sheets of white typing paper and (iii) one active timed manual method. The study was carried out in the Alto Chicama River Valley, Province of Gran Chimú, Department of La Libertad. The study design consisted of an initial searching of triatomines inside of the domestic environment by the manual capture active procedure (man/hour) covering all the studied houses. Then, matched pairs of GN boxes and paper sheets were simultaneously installed in the bedrooms of 207 households distributed in 19 localities. A comparative prospective trial of these passive detection devices were monitored at 2, 4 and, finally 6 months follow-up. Parasitological Trypanosoma rangeli and/or T. cruzi infections were investigated in two houses with high level of infestation by R. ecuadoriensis.16.9% of the 207 households investigated by an initial active manual method were infested with R. ecuadoriensis. The proportion of infested houses fluctuated from 6.2 to 55.5% amongst the 19 localities investigated. T. rangeli natural infection was detected in R.ecuadoriensis specimens collected in two households. Parasite rates in the bugs ranged from 16.6 to 21.7% respectively. The most striking fact was an average rate of salivary gland infection ranging from 7.4 to 8.3%. At the end of the sixth month period, a cumulative incidence of 31.4% of positive GN boxes against 15.9% for paper sheets was recorded. All three methods combined detected domestic infestation in 129 (62.3%) of the 207 houses studied in the 19 localities. The range of houses infested varies from 6.7% to 92.9%. In areas with low bug density infestation rates, the methodology experienced in our studies, seems to be the best choice for investigations on domestic R. ecuadoriensis populations.

The Promise of Access: Hope and Inequality in the Information Economy

In 2013, a series of posters began appearing in Washington, DC’s Metro system. Each declared “The internet: Your future depends on it” next to a photo of a middle-aged black Washingtonian, and an advertisement for the municipal government’s digital training resources. This hopeful discourse is familiar but where exactly does it come from? And how are our public institutions reorganized to approach the problem of poverty as a problem of technology? The Clinton administration’s ‘digital divide’ policy program popularized this hopeful discourse about personal computing powering social mobility, positioned internet startups as the ‘right’ side of the divide, and charged institutions of social reproduction such as schools and libraries with closing the gap and upgrading themselves in the image of internet startups. After introducing the development regime that builds this idea into the urban landscape through what I call the ‘political economy of hope’, and tracing the origin of the digital divide frame, this dissertation draws on three years of comparative ethnographic fieldwork in startups, schools, and libraries to explore how this hope is reproduced in daily life, becoming the common sense that drives our understanding of and interaction with economic inequality and reproduces that inequality in turn. I show that the hope in personal computing to power social mobility becomes a method of securing legitimacy and resources for both white émigré technologists and institutions of social reproduction struggling to understand and manage the persistent poverty of the information economy. I track the movement of this common sense between institutions, showing how the political economy of hope transforms them as part of a larger development project. This dissertation models a new, relational direction for digital divide research that grounds the politics of economic inequality with an empirical focus on technologies of poverty management. It demands a conceptual shift that sees the digital divide not as a bug within the information economy, but a feature of it.

Stomach fullness modulates prey size choice in the frillfin goby, Bathygobius soporator

Behaviours related to foraging and feeding in predator-prey systems are fundamental to our understanding of food webs. From the perspective of a predator, the selection of prey size depends upon a number of factors including prey vulnerability, prey size, and the predator's motivation to eat. Thus, feeding motivation and prey visual cues are supposed to influence predator decisions and it is predicted that prey selection by visual cues is modulated by the predator's stomach fullness prior to attacking a prey. This study was conducted using an animal model from the rocky shores ecosystem, a predatory fish, the frillfin goby Bathygobius soporator, and a benthic prey, the mottled shore crab Pachygrapsus transversus. Our results demonstrate that frillfin gables are capable of visually evaluating prey size and that the size evaluation process is modulated by the level of stomach fullness. Predators with an empty stomach (0% fullness) attacked prey that was larger than the predicted optimal size. Partially satiated predators (50% stomach fullness) selected prey close to the optimal size, while fully satiated predators (100% stomach fullness) showed no preference for size. This finding indicates an integrative response of the predator that depends on the input of both internal and external sensory information when choosing prey. Predator perceptions of visual cues (prey size) and stomach fullness modulate foraging decisions. As a result, a flexible feeding behaviour emerges, evidencing a clearly adaptive response in line with optimal foraging theory predictions. (C) 2012 Elsevier GmbH. All rights reserved.

First report of Ciguatoxins in two starfish species: Ophidiaster ophidianus and Marthasterias glacialis

Ciguatera fish poisoning (CFP) is a syndrome caused by the ingestion of fish contaminated with Ciguatoxins (CTXs). These phycotoxins are produced mainly by dinoflagellates that belong to the genus Gambierdiscus that are transformed in more toxic forms in predatory fish guts, and are more present in the Indo-Pacific and Caribbean areas. It is estimated that CFP causes per year more than 10,000 intoxications worldwide. With the rise of water temperature and anthropogenic intervention, it is important to study the prevalence of CFP in more temperate waters. Through inter- and subtidal sampling, 22 species of organisms were collected, in Madeira and Azores archipelagos and in the northwestern Moroccan coast, during September of 2012 and June and July of 2013. A total of 94 samples of 22 different species of bivalves, gastropods, echinoderms and crustaceans where analyzed by Ultra Performance Liquid Chromatography-Mass Spectometry-Ion Trap-Time of Flight (UPLC-MS-IT-TOF) and Ultra Performance Chromatography- Mass Spectrometry (UPLC-MS). Our main aim was to detect new vectors and ascertain if there were some geographical differences. We detected for the first time putative CTXs in echinoderms, in two starfish species—M. glacialis and O. ophidianus. We detected differences regarding uptake values by organisms and geographical location. Toxin amounts were significant, showing the importance and the need for continuity of these studies to gain more knowledge about the prevalence of these toxins, in order to better access human health risk. In addition, we suggest monitoring of these toxins should be extended to other vectors, starfish being a good alternative for protecting and accessing human health risk.

Classifying And Predicting Software Security Vulnerabilities based on Reproducibility

Security defects are common in large software systems because of their size and complexity. Although efficient development processes, testing, and maintenance policies are applied to software systems, there are still a large number of vulnerabilities that can remain, despite these measures. Some vulnerabilities stay in a system from one release to the next one because they cannot be easily reproduced through testing. These vulnerabilities endanger the security of the systems. We propose vulnerability classification and prediction frameworks based on vulnerability reproducibility. The frameworks are effective to identify the types and locations of vulnerabilities in the earlier stage, and improve the security of software in the next versions (referred to as releases). We expand an existing concept of software bug classification to vulnerability classification (easily reproducible and hard to reproduce) to develop a classification framework for differentiating between these vulnerabilities based on code fixes and textual reports. We then investigate the potential correlations between the vulnerability categories and the classical software metrics and some other runtime environmental factors of reproducibility to develop a vulnerability prediction framework. The classification and prediction frameworks help developers adopt corresponding mitigation or elimination actions and develop appropriate test cases. Also, the vulnerability prediction framework is of great help for security experts focus their effort on the top-ranked vulnerability-prone files. As a result, the frameworks decrease the number of attacks that exploit security vulnerabilities in the next versions of the software. To build the classification and prediction frameworks, different machine learning techniques (C4.5 Decision Tree, Random Forest, Logistic Regression, and Naive Bayes) are employed. The effectiveness of the proposed frameworks is assessed based on collected software security defects of Mozilla Firefox.

First report of Ciguatoxins in two starfish species: Ophidiaster ophidianus and Marthasterias glacialis

Ciguatera fish poisoning (CFP) is a syndrome caused by the ingestion of fish contaminated with Ciguatoxins (CTXs). These phycotoxins are produced mainly by dinoflagellates that belong to the genus Gambierdiscus that are transformed in more toxic forms in predatory fish guts, and are more present in the Indo-Pacific and Caribbean areas. It is estimated that CFP causes per year more than 10,000 intoxications worldwide. With the rise of water temperature and anthropogenic intervention, it is important to study the prevalence of CFP in more temperate waters. Through inter- and subtidal sampling, 22 species of organisms were collected, in Madeira and Azores archipelagos and in the northwestern Moroccan coast, during September of 2012 and June and July of 2013. A total of 94 samples of 22 different species of bivalves, gastropods, echinoderms and crustaceans where analyzed by Ultra Performance Liquid Chromatography-Mass Spectometry-Ion Trap-Time of Flight (UPLC-MS-IT-TOF) and Ultra Performance Chromatography- Mass Spectrometry (UPLC-MS). Our main aim was to detect new vectors and ascertain if there were some geographical differences. We detected for the first time putative CTXs in echinoderms, in two starfish species—M. glacialis and O. ophidianus. We detected differences regarding uptake values by organisms and geographical location. Toxin amounts were significant, showing the importance and the need for continuity of these studies to gain more knowledge about the prevalence of these toxins, in order to better access human health risk. In addition, we suggest monitoring of these toxins should be extended to other vectors, starfish being a good alternative for protecting and accessing human health risk.