银鲫与彩鲫卵母细胞cDNA文库构建及周期蛋白A_1的cDNA克隆

分别取行天然雌核发育繁殖的银鲫和两性生殖的彩鲫的卵母细胞为材料 ,提取总RNA ,分离mRNA ,进而反转录合成cDNA并定向插入λgtllSfi Not克隆载体 ,经体外包装构建了银鲫与彩鲫卵母细胞的表达型cDNA文库。测试结果表明库容量分别达到 3 1× 1 0 6(银鲫 )和 1 6× 1 0 6(彩鲫 )。进一步人工合成CyclinA1 保守引物 ,采用PCR扩增文库的方法 ,克隆了银鲫 (1 61 6bp)与彩鲫 (1 62 6bp)的CyclinA1 全长cDNA。序列分析结果表明 :两种鱼编

Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies

Genetically improved transgenic fish possess many beneficial economic traits; however, the commercial aquaculture of transgenic fish has not been performed till date. One of the major reasons for this is the possible ecological risk associated with the escape or release of the transgenic fish. Using a growth hormone transgenic fish with rapid growth characteristics as a subject, this paper analyzes the following: the essence of the potential ecological risks posed by transgenic fish; ecological risk in the current situation due to transgenic fish via one-factor phenotypic and fitness analysis, and mathematical model deduction. Then, it expounds new ideas and the latest findings using an artificially simulated ecosystem for the evaluation of the ecological risks posed by transgenic fish. Further, the study comments on the strategies and principles of controlling these ecological risks by using a triplold approach. Based on these results, we propose that ecological risk evaluation and prevention strategies are indispensable important components and should be accompanied with breeding research in order to provide enlightments for transgenic fish breeding, evaluation of the ecological risks posed by transgenic fish, and development of containment strategies against the risks.

One-legged locomotion with a compliant passive joint

There is an increasing attention of exploiting compliant materials for the purpose of legged locomotion, because they provide significant advantages in locomotion performance with respect to energy efficiency and stability. Toward establishing a fundamental basis for this line of research, a minimalistic locomotion model of a single legged system is explored in this paper. By analyzing the dynamic behavior of the system in simulation and a physical robotic platform, it is shown that a stable locomotion process can be achieved without the necessity of sensory feedback. In addition, further analysis characterizes the relation between motor control and the natural body dynamics determined by morphological properties such as body mass and spring constant. © 2006 The authors.

Formation of total-dose-radiation hardened materials by sequential oxygen and nitrogen implantation and multi-step annealing

Separation by implantation of oxygen and nitrogen (SIMON) silicon-on-insulator (SOI) materials were fabricated by sequential oxygen and nitrogen implantation with annealing after each implantation. Analyses of SIMS, XTEM and HRTEM were performed. The results show that superior buried insulating multi-layers were well formed and the possible mechanism is discussed. The remarkable total-dose irradiation tolerance of SIMON materials was confirmed by few shifts of drain leakage current-gate source voltage (I-V) curves of PMOS transistors fabricated on SIMON materials before and after irradiation.

基于身份的移动网动态群组密钥协商方案

群组密钥协商是群组通信中非常重要的基本工具，如何得到一个安全有效的密钥协商协议是当前密码学研究中的一个重要问题。基于双线性对和随机预言模型，针对移动网络提出了一个动态群组密钥协商方案。此方案就计算复杂度和通信复杂度而言都是高效的，而且满足密钥协商所需耍的安全要求。

黄土高原不同类型旱区旱作粮田深层土壤干燥化特征

随降水量趋势性减少和粮食产量不断提高,黄土高原旱作粮田深层土壤干燥化现象日益显现。在黄土高原不同类型旱区,测定了32类旱作粮田0~600 cm土层土壤湿度,分析和比较了各类粮田深层土壤贮水量、土壤湿度剖面分布和土壤干燥化强度。结果表明:①32类旱作粮田0~600 cm土层土壤湿度、土壤贮水量和土壤有效贮水量分别为13.90%、1084.4 mm和573.7 mm,其中有16类粮田发生了土壤干燥化现象,土壤水分过耗量平均值85.1 mm;②有28类旱作粮田100~400 cm土层为土壤干层,其中夏粮田土壤干层厚度大于秋粮田,最大耗水深度接近或超过600 cm;③32类旱作粮田和16类干燥化粮田土壤干燥化指数分别为110%和83%,分别属于无干燥化和轻度干燥化强度,土壤干层厚度平均值为267 cm,以半干旱偏旱区粮田土壤干燥化程度最严重。

聚丙烯酸钠对3种土壤持水能力及作物产量的影响

在离心机模拟不同水吸力条件下,研究了聚丙烯酸钠(sodium polyacrylate记作SP)5种使用浓度(占干土质量0,0.01%,0.08%,0.2%与1%)对3种土壤(砂土、壤土、黏土)持水能力的影响;采用大田试验研究了地表撒施2 g/m2SP对冬小麦与下季玉米产量及WUE影响。结果表明:3种土壤在0.01 MPa至1.5 MPa水吸力下的持水能力随着SP用量的增加而增加,砂土的作用效果较壤土、黏土更显著;3种土壤适宜浓度为0.08%~0.2%,最佳用量为0.2%,此用量条件下砂土、壤土、黏土的最大毛管持水量分别较对照增加了138.61%,7.22%,62.70%;不灌水条件下,SP处理较不施用SP冬小麦增产4%,WUE增加5.7%,灌浆期灌水28.5 mm条件下SP处理较不施用SP增产1%,WUE降低1%;SP处理的玉米产量较对照降低0.5%,WUE提高3%,效果明显低于对冬小麦效果。