Alterações no perfil dos lábios de pacientes submetidos a avanços maxilares em cirurgia ortognática do tipo Le Fort I

A literatura que descreve os resultados das correções cirúrgicas nos tecidos moles é limitada, pois apresenta significativa variação e não oferece conclusões definitivas. O propósito desse artigo é estudar retrospectivamente os efeitos das cirurgias ortognáticas maxilares tipo Le Fort I nos lábios superiores e inferiores de pacientes adultos e discutir os potenciais problemas envolvidos com a metodologia aplicada. Foram estudados 19 pacientes brancos de 19 a 42 anos de idade, sendo 10 homens e 9 mulheres, submetidos a cirurgias maxilares no Centro de Pesquisa e Tratamento das Deformidades Buco-Faciais - CEDEFACE (Araraquara, SP). A grande maioria dos casos apresentou, no mínimo, uma discrepância significativa, seja ântero-posterior ou vertical, reforçando a sua indicação cirúrgica. As alterações nos tecidos moles foram significativas quando as movimentações horizontais ou verticais da base óssea maxilar foram significativas, com uma relação de aproximadamente 0,6:1 entre as alterações ântero-posteriores do lábio superior e a movimentação da base óssea. Alterações verticais na maxila, sem significativas alterações horizontais, não foram suficientes para aumentar verticalmente o lábio superior. Ocorreram alterações ântero-posteriores do lábio inferior em conseqüência do deslocamento anterior e inferior da maxila, o que permitiu um melhor selamento labial. Alterações verticais não foram quantificadas no lábio inferior.

INFLUENCE OF ENTERAL PARASITES ON THE BLOOD VITAMIN-A LEVELS IN PRESCHOOL-CHILDREN ORALLY SUPPLEMENTED WITH RETINOL AND OR ZINC

A sample of 471 pre-school children who frequented schools and creches in a poor district of Manaus (Amazonas), Brazil, were randomly submitted to faecal parasitological tests. Two-hundred-and-forty children from both sexes between the ages of 3 and 7 years with Ascaris lumbricoides and/or Giardia lamblia were selected. The objective of the study was to determine the possible influence of these two intestinal parasites and vitamin A and/or zinc supplementation on the serum retinol levels of primary school children. The children were submitted to clinical and anthropometric examinations, dietary interviews and biochemical examinations of retinol and carotene in the serum and of zinc in the hair. The parasitic incidence was 85.0% and about 54% of the children were polyparasitic. During the pretreatment phase, the retinol and carotene serum levels were 36% and 57%, respectively, below the normal levels. Using the Waterlow classification, the anthropometric analyses revealed that 88% of the children showed normal growth. A significant effect was observed of the anti-parasitic medicine on the serum retinol levels.

Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds

A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8% (w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 angstrom resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.

Lattice discretization in quantum scattering

The utility of lattice discretization technique is demonstrated for solving nonrelativistic quantum scattering problems and specially for the treatment of ultraviolet divergences in these problems with some potentials singular at the origin in two- and three-space dimensions. This shows that the lattice discretization technique could be a useful tool for the numerical solution of scattering problems in general. The approach is illustrated in the case of the Dirac delta function potential.

RESISTANCE OF TRYPANOSOMA-CRUZI TO BLOOD CLEARANCE INDUCED BY ACUTE-PHASE IMMUNE MOUSE SERUM

To investigate functional changes in Trypanosoma cruzi parasites induced during their interaction with the vertebrate host, we compared the blood clearance profiles of blood forms isolated from infected normal mice (Reg-Tc) or from infected mice immunodepressed after treatment with cyclophosphamide (Cy-Tc). Parasite blood numbers were measured at various time intervals in animals injected intravenously (i.v.) with 1-2 x 10(6) T. cruzi of either isolate. In the absence of added immune sera (spontaneous clearance), Reg-Tc and Cy-Tc were cleared from blood at similar rates. However, when acute immune mouse serum (Ac-IMS) was injected i.v. 2 min after inoculation of parasites, a significant proportion of Cy-Tc only was cleared from the blood an hour later, whereas Reg-Tc were not, their clearance profile being identical to that observed in mice injected with normal mouse serum. Cy-Tc susceptibility to Ac-IMS was not the result of a toxic effect of cyclophosphamide over T. cruzi as parasites recovered from animals immunodepressed by irradiation before infection were cleared similarly by acute serum. Contrary to Ac-IMS, chronic immune mouse serum induced similar rates of disappearance of Reg-Tc and Cy-Tc from blood. Our results suggest the occurrence of T. cruzi selection or modification during the acute phase, which leads to an increased parasite resistance to the clearance properties of acute-phase antibodies.

Seroepidemiology of Toxoplasma gondii, Neospora caninum, and Leishmania spp. infections and risk factors for cats from Brazil

The seroprevalence of infection by Toxoplasma gondii, Neospora caninum, and Leishmania spp. was detected through an indirect immunofluorescence in 70 cats from the Andradina Municipality, São Paulo State, Brazil. Anti-T. gondii antibodies (titer >64) were detected in 15.7% (11/70) of animals, whereas positivity for N. caninum (titer 16) was not observed in any animal. of the cats from urban and rural areas, 10.4% (5/48) and 27.2% (6/22) were positive for T. gondii, respectively. Breed, age, food, and contact with animals of other species were significant for considering the positivity for T. gondii (P <= 0.0001). Cats having access to streets (17.1%, 11/64), cats cohabiting with rats (19.6%, 10/51), and cats feeding on homemade food and raw milk (27.2%, 6/22) were positive for T. gondii. In addition, 4.2% (3/70) of the cats were positive for Leishmania spp. by ELISA technique and negative by IFAT without coinfection with T. gondii and Leishmania spp. There was no serological positivity against feline immunodeficiency virus or feline leukemia virus. In conclusion, T. gondii infection in part of the feline population from Andradina is not linked to immunosuppressions or coinfections but probably to postnatal infection in association with the type of diet and presence of rats.

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Viabilidade do tratamento de água residuária sintética têxtil em reator aeróbio de leito fixo

A continuous flow reactor, inoculated with Aspergillus niger AN400, with total volume of 5 L was operated at 29 degrees C, with eight hours of retention hydraulic time and 150 L.h(-1) of air flow rate in order to remove 25 mg.L(-1) of Congo Red dye from a synthetic wastewater. The feeding of the reactor, inoculated with Aspergillus niger AN400, was done in two phases: Phase I, with 0,5 g/L of saccharose and Phase II, with no saccharose. In Phase I, it was possible to verify efficiencies of organic matter and color (mg Pt.L(-1)) removal of 80 +/- 16% and 82 +/- 10%, respectively. In Phase II, the efficiency of organic matter removal was 75 +/- 13% and color removal was 89 +/- 7%. The higher removals of nutrients were achieved by the reactor in Phase I with 25% to ammonia, 90% to nitrite, 93% to nitrate and 21% to phosphorus. Apparently, the presence of saccharose improved the removal of the nutrients.

Potencial alelopático e levantamento do banco natural de sementes sob a copa de Copaifera langsdorffii Desf

the aim of this study was to evaluate the allelopathic effect of soil samples collected under the canopies of three specimens of Copaifera langsdorffii the germination of Lactuca sativa and survey the natural seed bank according to seasonality. To test the allelopathic effect was carried experiments of pre and post-emergence with seeds of L. sativa and to quantify the stock of seeds, soil samples were collected from three specimens at three distances from the stem (1, 2 and 3 m) and at three depths (0-5, 5-10 and 10-15cm) in the region of savanna in the dry and wet seasons. The samples tested in bioassay of pre-emergence no significant influence on germinability, mean germination time and mean germination speed, but showed a difference in the synchronism of germination, these data were independent of sampling station. In test for post-emergence was observed statistical difference in the parameters evaluate (length of primary roots and hypocotyls) in both seasons. To quantify the natural seed bank were macroscopic analysis of each soil sample with the help of stereoscopic microscope. The analysis of the natural seed bank showed a larger number of seeds in the 0-5cm and in distance of 2m for both seasons. The results suggest the presence of allelochemical substances in soil samples collected under the canopy of Copaifera langsdorffii.

Moenkhausia celibela: a new species from the Amazon basin, Brazil (Characiformes: Characidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito das condições climáticas do semiárido sobre o comportamento fisiológico de caprinos mestiços f1 saanen x boer

This research aimed at evaluating the effect of the semiarid climatic conditions on the physiological behavior of F1 Saanen x Boer crossbred goats, created at an intensive system. The experiment was carried out in the Health and Rural Technology Center, in the Federal University of Campina Grande, in the town of Patos, Paraiba. Twenty-four animals were used, with ages varying between three and four months, being 12 females and 12 males, weaned and prevented against worms, receiving ad libitum water and controlled concentrate and roughage. The environmental variables were checked inside and outside the experiment place, as well as the physiological variables of all animals. Except for the humid bulb temperature, the variance analysis revealed shift effect (P<0.05) for all environmental variables. There was merely shift effect (P<0.05) for Cardiac Frequency and Rectal Temperature, and the averages of both, in the afternoon, overcame those observed in the morning. The obtained results based on the studied physiological and environmental variables conclude that crossbred goats, resulting of Boer (paternal) and Saanen (maternal), present a good resistance to heat, permitting their indication for the meat production in confinement on semiarid conditions.

Purification of a PHA-Like chitin-binding protein from Acacia farnesiana seeds: A time-dependent oligomerization protein

A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO erythrocytes either native or treated with proteolytic enzymes. In sodium dodecyl sulfate gel electrophoresis under reducing and nonreducing conditions, AFAL separated into two bands with a subunit molecular mass of 35 and 50 kDa. The homogeneity of purified protein was confirmed by chromatofocusing with a pI=4.0+/-0.5. Molecular exclusion chromatography confirmed time-dependent oligomerization in AFAL, in accordance with mass spectrometry analysis, which confers an alteration in AFAL affinity for chitin. The protein sequence was obtained by a liquid chromatography quadrupole time-of-flight experiment and showed that AFAL has 68% and 63% sequence similarity with lectins of Phaseolus vulgaris and Dolichos biflorus, respectively.