Morphological and cytochemical changes in synganglion of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) female ticks from exposure of andiroba oil (Carapa guianensis)

Actually, the most used method to control ticks is synthetic acaricides with neurotoxic action. However, the use of these methods presents inconveniences, such as the contamination of the environment and risks to the host's health due to the residual effects. Thus, several studies have been developed aiming to find alternative ways to control these ectoparasites, such as the use of natural compounds with active ingredients, which act controlling some species of plagues in addition to presenting medicinal properties that are beneficial to humans. The present study aimed to analyze the action of andiroba oil (Carapa guianensis) on the synganglion of Rhipicephalus sanguineus semiengorged females through morphological and cytochemical techniques aiming to verify if this natural product have neurotoxic action as the numerous synthetic acaricides. The results showed that andiroba oil interferes in the synganglion through structural and enzymatic changes, which lead the nervous tissue to apoptotic death involving autophagy. Among these changes was observed the emergence of large empty spaces between the perineurium and the cortical region, vacuolated cortex cells and with cell swelling, neural cells with picnotic nuclei or in initial stage of chromatin margination and neuropile with high structural disorganization. Considering these data, it can be concluded that andiroba seed oil can be used as an alternative method in the control of R. sanguineus ticks due to its neurotoxic action. © 2013 Wiley Periodicals, Inc.

Histopathology of the tegument of rabbits infested by Rhipicephalus sanguineus (ACARI: IXODIDAE) ticks and exposed to selamectin (active principle of acaricide Revolution®, Pfizer)

Ticks are hematophagous ectoparasites which can transmit several diseases to the host during their feeding process. When ticks mechanically damage the tissue, they eventually induce inflammatory responses on the skin spot where they are fixed. One of the alternatives to control these ectoparasites is the use of chemical substances like selamectin - the active principle of Pfizer's antiparasitic Revolution® - a macrocyclic lactone capable of doing neurotoxic damage to the tick and eventually eliminating infestation in dogs and cats. The purpose of this study was to analyze, using histological and histochemical techniques, the occurrence of morphophysiological alterations in the skin of the host rabbits exposed to selamectin and infested with Rhipicephalus sanguineus (Acari: Ixodidae). Histologically, the exposed and infested rabbits showed a partial and/or total decrease in the stratum corneum and the epithelium decreased in the number of cell layers, consequently reducing the stratification (thinning) and quite pronounced formations of sub-epidermal edemas with consequent disorganization of collagen fibers in the dermal layer's connective tissue. Histochemical tests showed strong periodic acid-Schiff-positive reaction in the hair follicle and some regions of the dermis, besides resynthesis of collagen fibers detected by Mallory's trichrome technique. The obtained results showed that selamectin acts like a toxicant agent when in contact with the skin of the rabbit infested with ticks, inducing morphophysiological alterations in the acute inflammatory process in the animal's tegument. Selamectin is a chemical substance which has a dose-dependent action since higher concentrations cause greater morphophysiological damage in the skin of rabbits. © 2013 Springer-Verlag Berlin Heidelberg.

Morphology of the female genital ducts of the blue land crab Cardisoma guanhumi (Crustacea: Brachyura: Gecarcinidae)

This is a histological and histochemical analysis of the terminal portion of the female reproductive system and genital ducts of the blue land crab (Cardisoma guanhumi). Animals were collected in the Jaguaribe estuary (Ceará, Brazil) and dissected. Genital duct fragments were fixed and submitted to different staining techniques. The female reproductive system consists of a pair of ovaries and a pair of genital ducts. In the mid-posterior portion of each lobe, the ovaries communicate with the genital ducts, which are subdivided into oviduct, spermatheca, vagina, and gonopore. Histologically, the spermatheca of C. guanhumi is composed of columnar secretory epithelium and is divided into a dorsal zone and a ventral zone, the latter covered internally by a cuticle layer. Both zones are enveloped by a thin layer of loose connective tissue. Histological cross sections revealed the vagina to be concave, a pattern considered phylogenetically more advanced than the simple, tubular form. Our findings suggest fertilization is internal, favoring sperm from the most recent copulation. © 2012 The Authors. Acta Zoologica © 2012 The Royal Swedish Academy of Sciences.

Search for physics beyond the standard model in events with τ leptons, jets, and large transverse momentum imbalance in pp collisions at √s = 7 TeV

A search for physics beyond the standard model is performed with events having one or more hadronically decaying τ leptons, highly energetic jets, and large transverse momentum imbalance. The data sample corresponds to an integrated luminosity of 4.98 fb-1 of proton-proton collisions at √ = 7 TeV collected with the CMS detector at the LHC in 2011. The number of observed events is consistent with predictions for standard model processes. Lower limits on the mass of the gluino in supersymmetric models are determined. © 2013 CERN for the benefit of the CMS collaboration.

Measurement of masses in the tt̄ system by kinematic endpoints in pp collisions at √s = 7 TeV

A simultaneous measurement of the top-quark, W-boson, and neutrino masses is reported for tt̄ events selected in the dilepton final state from a data sample corresponding to an integrated luminosity of 5.0 fb-1 collected by the CMS experiment in pp collisions at √s = 7 TeV. The analysis is based on endpoint determinations in kinematic distributions. When the neutrino and W-boson masses are constrained to their world-average values, a top-quark mass value of Mt = 173.9 ± 0.9 (stat)+1.7 -2.1(syst.) GeV is obtained. When such constraints are not used, the three particle masses are obtained in a simultaneous fit. In this unconstrained mode the study serves as a test of mass determination methods that may be used in beyond standard model physics scenarios where several masses in a decay chain may be unknown and undetected particles lead to underconstrained kinematics. © 2013 CERN for the benefit of the CMS collaboration.

The action of aminoguanidine on the liver of trained diabetic rats

Background: This study evaluated the effect of aminoguanidine on liver of diabetic rats subject to physical exercises using histological and histochemical techniques.Methods: The rats used in this study were divided into five groups: sedentary control, sedentary diabetic, trained diabetic, sedentary diabetic and treated with aminoguanidine, trained diabetic and treated with aminoguanidine.Results: The results showed no effect of aminoguanidine on the liver tissue, although there was improvement with exercise training showing cytological, morpho-histological and histochemical alterations in liver cells of animals from groups trained diabetic and/or treated diabetic compared to those individuals in the sedentary control and sedentary diabetic. These changes included: hepatocytes hypertrophy, presence and distribution of polysaccharides in the hepatocytes cytoplasm and, especially, congestion of the liver blood vessels.Conclusion: Our results suggest that aminoguanidine is not hepatotoxic, when used at dosage of 1 g/L for the treatment of diabetes complications, and confirmed that the practice of moderate physical exercise assuaged the damage caused by diabetes without the use of insulin. © 2013 e Nico et al.; licensee BioMed Central Ltd.

Searches for Higgs bosons in pp collisions at s=7 and 8 TeV in the context of four-generation and fermiophobic models

Searches are reported for Higgs bosons in the context of either the standard model extended to include a fourth generation of fermions (SM4) with masses of up to 600 GeV or fermiophobic models. For the former, results from three decay modes (ττ, WW, and ZZ) are combined, whilst for the latter the diphoton decay is exploited. The analysed proton-proton collision data correspond to integrated luminosities of up to 5.1 fb-1 at 7 TeV and up to 5.3 fb-1 at 8 TeV. The observed results exclude the SM4 Higgs boson in the mass range 110-600 GeV at 99% confidence level (CL), and in the mass range 110-560 GeV at 99.9% CL. A fermiophobic Higgs boson is excluded in the mass range 110-147 GeV at 95% CL, and in the range 110-133 GeV at 99% CL. The recently observed boson with a mass near 125 GeV is not consistent with either an SM4 or a fermiophobic Higgs boson. © 2013 CERN.

Multiplicity and transverse momentum dependence of two- and four-particle correlations in pPb and PbPb collisions

Measurements of two- and four-particle angular correlations for charged particles emitted in pPb collisions are presented over a wide range in pseudorapidity and full azimuth. The data, corresponding to an integrated luminosity of approximately 31nb-1, were collected during the 2013 LHC pPb run at a nucleon-nucleon center-of-mass energy of 5.02 TeV by the CMS experiment. The results are compared to 2.76 TeV semi-peripheral PbPb collision data, collected during the 2011 PbPb run, covering a similar range of particle multiplicities. The observed correlations are characterized by the near-side (|δφ|≈0) associated pair yields and the azimuthal anisotropy Fourier harmonics (vn). The second-order (v2) and third-order (v3) anisotropy harmonics are extracted using the two-particle azimuthal correlation technique. A four-particle correlation method is also applied to obtain the value of v2 and further explore the multi-particle nature of the correlations. Both associated pair yields and anisotropy harmonics are studied as a function of particle multiplicity and transverse momentum. The associated pair yields, the four-particle v2, and the v3 become apparent at about the same multiplicity. A remarkable similarity in the v3 signal as a function of multiplicity is observed between the pPb and PbPb systems. Predictions based on the color glass condensate and hydrodynamic models are compared to the experimental results. © 2013 CERN.

Observation of a new boson with mass near 125 GeV in pp collisions at √=7 and 8 TeV

A detailed description is reported of the analysis used by the CMS Collaboration in the search for the standard model Higgs boson in pp collisions at the LHC, which led to the observation of a new boson. The data sample corresponds to integrated luminosities up to 5.1 fb-1 at √=7 TeV, and up to 5.3 fb-1 at √ s=8 TeV. The results for five Higgs boson decay modes γγ, ZZ, WW, ττ, and bb, which show a combined local significance of 5 standard deviations near 125 GeV, are reviewed. A fit to the invariant mass of the two high resolution channels, γγ and ZZ → 4ℓ, gives a mass estimate of 125.3 ± 0.4 (stat.) ± 0.5 (syst.) GeV. The measurements are interpreted in the context of the standard model Lagrangian for the scalar Higgs field interacting with fermions and vector bosons. The measured values of the corresponding couplings are compared to the standard model predictions. The hypothesis of custodial symmetry is tested through the measurement of the ratio of the couplings to the W and Z bosons. All the results are consistent, within their uncertainties, with the expectations for a standard model Higgs boson. [Figure not available: see fulltext.] © 2013 CERN for the benefit of the CMS collaboration.

Searches for long-lived charged particles in pp collisions at □ =7 and 8 TeV

Abstract: Results of searches for heavy stable charged particles produced in pp collisions at □ = 7 and 8 TeV are presented corresponding to an integrated luminosity of 5.0 fb-1 and 18.8 fb-1, respectively. Data collected with the CMS detector are used to study the momentum, energy deposition, and time-of-flight of signal candidates. Leptons with an electric charge between e/3 and 8e, as well as bound states that can undergo charge exchange with the detector material, are studied. Analysis results are presented for various combinations of signatures in the inner tracker only, inner tracker and muon detector, and muon detector only. Detector signatures utilized are long time-of-flight to the outer muon system and anomalously high (or low) energy deposition in the inner tracker. The data are consistent with the expected background, and upper limits are set on the production cross section of long-lived gluinos, scalar top quarks, and scalar τ leptons, as well as pair produced long-lived leptons. Corresponding lower mass limits, ranging up to 1322 GeV/c 2 for gluinos, are the most stringent to date. [Figure not available: see fulltext.] © 2013 Cern for the benefit of the CMS collaboration.

Study of exclusive two-photon production of W+W- in pp collisions at √s=7 TeV and constraints on anomalous quartic gauge couplings

A search for exclusive or quasi-exclusive W+W- production by photon-photon interactions, pp → p(*)W +W-p(*), at √s=7 TeV is reported using data collected by the CMS detector with an integrated luminosity of 5.05 fb-1. Events are selected by requiring a μ ±e∓ vertex with no additional associated charged tracks and dilepton transverse momentum p T(μ ±e∓) > 30 GeV. Two events passing all selection requirements are observed in the data, compared to a standard model expectation of 2.2 ± 0.4 signal events with 0.84 ± 0.15 background. The tail of the dilepton p T distribution is studied for deviations from the standard model. No events are observed with p T > 100 GeV. Model-independent upper limits are computed and compared to predictions involving anomalous quartic gauge couplings. The limits on the parameters α0,C W/λ2 with a dipole form factor and an energy cutoff Λcutoff = 500 GeV are of the order of 10-4. © 2013 CERN for the benefit of the CMS collaboration.

Measurement of the λb 0 lifetime in pp collisions at √s=7 TeV

A measurement of the λb 0 lifetime using the decay λb 0-1, was recorded with the CMS experiment at the Large Hadron Collider using triggers that selected dimuon events in the J/ψ mass region. The λb 0 lifetime is measured to be 1.503 ± 0.052 (stat.) ± 0.031 (syst.) ps. [Figure not available: see fulltext.] © 2013 Cern for the benefit of the CMS collaboration.

Inclusive search for supersymmetry using razor variables in pp collisions at √s=7 TeV

An inclusive search is presented for new heavy particle pairs produced in √s=7 TeV proton-proton collisions at the LHC using 4.7±0.1 fb -1 of integrated luminosity. The selected events are analyzed in the 2D razor space of MR, an event-by-event indicator of the heavy particle mass scale, and R, a dimensionless variable related to the missing transverse energy. The third-generation sector is probed using the event heavy-flavor content. The search is sensitive to generic supersymmetry models with minimal assumptions about the superpartner decay chains. No excess is observed in the number of events beyond that predicted by the standard model. Exclusion limits are derived in the CMSSM framework as well as for simplified models. Within the CMSSM parameter space considered, gluino masses up to 800 GeV and squark masses up to 1.35 TeV are excluded at 95% confidence level depending on the model parameters. The direct production of pairs of top or bottom squarks is excluded for masses as high as 400 GeV. © 2013 CERN.

Search for microscopic black holes in pp collisions at √s=8 TeV

A search for microscopic black holes and string balls is presented, based on a data sample of pp collisions at √s=8 TeV recorded by the CMS experiment at the Large Hadron Collider and corresponding to an integrated luminosity of 12 fb-1. No excess of events with energetic multiparticle final states, typical of black hole production or of similar new physics processes, is observed. Given the agreement of the observations with the expected standard model background, which is dominated by QCD multijet production, 95% confidence level limits are set on the production of semiclassical or quantum black holes, or of string balls, corresponding to the exclusions of masses below 4.3 to 6.2 TeV, depending on model assumptions. In addition, model-independent limits are set on new physics processes resulting in energetic multiparticle final states. [Figure not available: see fulltext.] © 2013 CERN for the benefit of the CMS collaboration.

Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817

This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads). The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil. Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive). The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids). Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli. In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules. The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells. The bound calcium was mainly found in the F and M cells. The F cells showed strong positive staining for lipid while the R cell only stained weakly. The E cells did not react to any of the applied staining techniques. The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis. Also, they may be more related to the molt stage, as in the case of calcium salts. © 2013 Elsevier GmbH. All rights reserved.