Skipjack tuna (K. pelamis, L.) resources in the seas around Ceylon

Details are given of the source and processing of data, size composition, seasonal variation in occurrence, abundance and oceanic potential of Katsuwonus pelamis resources in the seas around Sri Lanka. The indirect evidences are indicative of the presence of skipjack resource in the oceanic range around the island, particularly the south and west of Sri Lanka.

Evaluation of quality of shark livers using bio-chemical properties and organoleptic score sheet

Shark livers are considered as an important raw material providing a quality fish oil. It has been reported to aid white — blood-cell production and act as an active ingredient in hemorrhoid treatments. It is also reported that liver oil as a good supplement of vitamin A and poly-unsaturated fatty acids which are important to the development of brain cells in human. Freshness of livers is very important to extract better quality oil. In Sri Lanka, the annual shark production amounts to 8000t, however the quality of livers collected from landing sites has not being measured yet. Present study was conducted to evaluate the quality of silky (Charcarninus fakiformis) shark livers available in Negombo and Beruwala landing sites in the West Coast of Sri Lanka and also to study the relationship between organoleptic and bio-chemical correlation on freshness of shark livers. Liver samples which were collected from landing sites in the West coast of Sri Lanka, were evaluated for external and internal colour, texture and odour. Total volatile nitrogen (TVN), pH value, free fatty acid (FFA%) and peroxide (PV) values of livers were also determined to assess quality. According to the organoleptic scoring system 4.3% of liver samples were categorized as best in quality while 30.4%, 56.5% and 8.7% rated as good, medium and poor in quality respectively at the Negombo and Beruwala landing sites. Bio-chemical analysis showed that the better quality livers had the highest score for sensory evaluation and low values for TVN, FFA and peroxide value while low quality livers gave low score for sensory evaluation and high TVN, FFA, peroxide values. Correlation coefficient of organoleptic scores against total volatile nitrogen value, pH value, free fatty acid % and peroxide value of shark livers were determined by statistical analysis. Organoleptic score of shark livers was found to be highly.

The oxidation of CH2O in the intermediate temperature range (943-995 K)

New atmospheric pressure flow reactor data on the oxidation of formaldehyde in the temperature range 943-995 K and over equivalence ratios from 0.013 to 36.7 are reported and discussed. A detailed mechanism assembled from previously published results produced acceptable agreement with the experimental data for the fuel-lean conditions, but failed to predict results for oxidative pyrolysis. Analysis or the very fuel-lean conditions, but failed to modelling results are principally sensitive to CH2O+HO2→HCO+H2O2 (6) and H2O2 +M→OH+OH+M (33). Whereas the specific rate of each reaction cannot be independently determined, it is found that the product k33.k6 is a well defined function of temperature: (3.4±3.0).1028 exp(-(26,800±400)/T). Inadequacies in the mechanism which may be responsible for the disagreement under fuel-rich conditions are discussed. © 1991 Combustion Institute.

Effects of different dietary levels of vitamin E on the breeding performance of Heteropneustes fossilis (Bloch)

Experiments on the study of different dietary levels of vitamin E on the growth and breeding performance of Heteropneustes fossilis brood fish were carried out in two phases. The first phase consisted of studying its ovarian development and the second phase on breeding performance. Sixty female fishes were stocked in twelve experimental chambers of a raceway. The effects of four dietary vitamin E levels viz. 0 (served as control), 50, 100 and 200 mg/kg feed, on the somatic growth, ovarian development of brood fish and on their breeding performance were studied. Each treatment had three replications. It was observed that body growth in terms of length and weight was best with 0 mg vitamin E/kg feed and 200 mg vitamin E/kg of feed gave poorest result. The gonado-somatic index and fecundity, however, was highest in the fish fed with 100 mg vitamin E/kg of feed. In case of breeding performance such as ovulation rate, fertilization rate, hatching rate and survival rate, the best result was obtained with 200 mg vitamin E/kg of feed. The overall result of this experiment indicates that 200 mg vitamin E/kg of feed is the best vitamin E dose for H fossilis brood and vitamin E content has a positive impact on ovarian development.

Development of artificial breeding techniques for long-whiskered catfish, Sperata aor and giant river catfish, Sperata seenghala of Bangladesh

Sperata aor and S. seenghala are the two important native catfishes of Bangladesh but commercial farming of these species is not possible due to lack of naturally collected or artificially produced seeds for stocking. Attempts were made to develop techniques for seed production by artificial breeding and nursery-rearing of fries of these catfishes. A total of 60 S. seenghala (750-1,500 g) and 10 S. aor (600-1,000 g) broods were collected from the Brahmaputra river-basin and floodplains in Mymensingh region four months prior to their breeding season. The collected brood fishes were reared in separate earthen ponds with supplementary feeds comprising of rice bran (40%), mustard oil cake (29%), fish meal (30%) and vitamin-premix (1 %). Three experiments were conducted to optimize the hormone dose. A total of nine S. seenghala females weighing from 750 to 1,500 g were given an initial and resolving dose of 12-20 and 16-24 mg PG/kg body weight, respectively. The males weighing from 650-950 g were administered a single dose of 18-26 mg PG/kg body weight at the time of the time of administering the resolving dose to the females. The females ovulated partially and the eggs were examined under a compound microscope, but most of them were found to be less ripe or damaged. Collection of milt by stripping the males was not successful. The testes were taken out and sperm were observed to be non-motile and less developed. In view of stimulating natural propagation of S. seenghala, artificial holes (nests) were constructed in the pond bottom. Each hole was 0.7 m in diameter and 0.3 m in depth. A total of 10 holes were made and then 10 pairs of S. seenghala breeders (800-1,200 g) were stocked in the pond. In mid February, 3,000 fry of S. seenghala with a mean length of 4.60 cm and weight of 0.36 g were collected by repeated netting followed by drying of the pond. The fry were then stocked in a nursery pond and fed with commercial feed (SABINCO starter-1). The average length and weight of the fingerlings were 9.01 cm and 3.95 g, respectively and the estimated survival was 60% after two months of rearing. S. aor did not respond to natural spawning. Further study is essential to develop techniques for their successful artificial and natural breeding.

Effect of thermal pollution on the hydrological parameters of River Jhelum (J & K)

The Buniyar hydroelectric project and the lower Jhelum Barrier at Gantamulla constructed across river Jhelum are in operation from several years. The two power stations have changed the hydrological features of the area where dam is situated. Therefore, the impact of hydroelectric projects on fishery resources of river Jhelum is a matter of great concern. The treated water from the power house is released through turbines, having cooling effect on them. The change in the hydrological parameters such as increase in temperature, depletion of oxygen etc. might have brought fishes under stress which is the main cause in decreased population of finfishes from site to site. The golden mahseer, Tor putitora which was once an abundant species in river Jhelum has disappeared due to human intervention. The prevailing water quality around hydroelectric projects is unsuitable for finfishes of river Jhelum.

Effect of dietary supplementation of tocoferol acetate alone and with varying combinations on growth, survival and fatty acids profile of Macrobrachium rosenbergii larvae through Moina micrura enrichment

A study was carried out to determine the effect of tocopherol acetate along with cod liver oil astaxanthin enriched Moina micrura (MC- control, Ml- tocopherol acetate enriched, M2-tocopherol acetate combined with cod liver oil (CLO) enriched and M3- tocopherol acetate combined with astaxanthin enriched) on growth, survival and fatty acid composition of M. rosenbergii (de Man) larvae (TC- unenriched Moina fed larvae, Tl- tocopherol acetate enriched Moina fed larvae, T2- tocopherol acetate + CLO enriched Moina fed larvae to T3 – tocopherol acetate+ astaxanthin enriched Moina fed larvae). Growth was expressed as the time taken in to the settlement of 95% post larvae. Maximum growth i.e., the lowest time taken to the 95% PL settlement (40 days) and the maximum survival percentage (61%) was observed in both T2 and T3 treatments fed with M2 and M3 Moina respectively. Minimum growth and survival was observed in unenriched Moina fed larvae (TC). In larval treatments T2, (larvae fed with (M2) vitamin E + CLO enriched Moina), showed a higher percentage of EPA, DHA and higher HUFA level than other treatments.

Effects of dietary vitamin E on the growth and breeding performance of Ompok pabda

A 90 day feeding trail was conducted to investigate the effect of vitamin E on the growth and breeding performance of Ompok pabda. A total of 84 healthy female brood fish (41.10±0.44g) were divided into 4 treatments i.e. treatment T1 T2, T3 and T4 having three replications each. The fish were fed twice a day with a standard feed (40% protein) having 4 doses of vitamin E viz. 0 (served as control), 50, 100 and 150mg vitamin E/kg feed. At the end of the feeding trial, the brood fish were induced to breed with PG extract to observe the effect of vitamin Eon feed. After rearing for 90 days with the experimental feeds, it was found that weight gain and specific growth rate of brood fish fed with 100mg vitamin E/kg feed (treatment T3) was the highest (14.78±0.38g and 2.99±0.11) while 150mg vitamin E/kg feed (treatment T4) fed fish gave the poorest result (2.97±0.89g and 1.21±0.32). There was no significant difference in terms of length gain of brood fish among the different treatments. The brood fish were induced to breed with equal dose of PG extract (18 and 12mg PG/kg body weight for female and male respectively) to observe the dietary effect of vitamin E on breeding performance. The highest ovulation, fertilization and hatching rate of eggs were found to be 81.48±6.41%, 84.04±3.53% and 68.59±5.03% respectively in the brood fish of treatment T1 while the poorest (33.33±00%, 52.35±5.02% and 45.70±7.24% respectively) were found in the brood fish under treatment T4. The results suggest that inclusion of 100mg vitamin E/kg feed is best for enhancing the breeding performance of O. pabda brood fish indicating that vitamin E content has a positive impact on reproduction of fish. The present results also imply that inclusion of higher level of vitamin E exerts an antagonistic effect in terms of growth and breeding performance of this species.

单核细胞、N K细胞和T 细胞在猪-猕猴延迟性异种移植排斥反应中的作用

　目的　观察单核细胞、NK细胞和T 细胞在猪2猕猴延迟性异种移植排斥反应(DXR) 中的作用。方法　建立湖北白猪2云南猕猴的腹腔异位心脏移植模型,实验分为2 组:对照组( n = 5) ,不使用中华眼睛蛇毒因( Y2CVF) ;实验组( n = 4) 应用Y2CVF 完全清除受者体内补体。2 组受 体猴均采用环孢素A(CsA) ,环磷酰胺(CTX) 和甲基强的松龙(MP) 三联免疫抑制治疗。免疫组织 化学方法检测移植心组织中细胞间黏附分子( ICAM)21 、肿瘤坏死因子( TNF)2α、单核细胞、NK 细 胞和T 细胞的表达。结果　对照组3 个移植心在15～60 min 内发生超急性排斥反应(HAR) ,另2 个分别存活22 h 及6 d ,移植心均未见明显的炎性细胞浸润及ICAM21 和TNF2α的表达。实验组 移植心存活时间分别为8 、10 、13 和13 d ,移植物浸润细胞中可见大量的单核细胞(50 %) ,少量的 NK细胞(8 %～10 %) ,CD4 + T 细胞(15 %) 和CD8 + T 细胞(25 %) 。移植物血管内皮细胞表面出现 ICAM21 的表达上调,移植物间质中出现TNF2α的表达增加。结论　单核细胞、NK细胞和T 细胞 介导的移植物损伤,在应用Y2CVF 处理的猪2猕猴DXR 发生中发挥重要作用　

Merging effects of vitamin E and C on biological factors, blood characteristics and resistance against thermal stress in rainbow trout (Onchorhyncus mykiss)

This study was conducted to assay the effects of different levels of dietary vitamins C and E on growth indices and survival and resistance against thermal stress of rainbow trout (Oncorhynchus mykiss) in pond culture of Marzan abad from December 2011 to February 2011. Seven diets were supplemented. 300 fish with the average weight of 17 g were introduced to ponds for 60 days. The results showed that the highest and the lowest weight gain were in fish fed with diet containing 50 mg/kg vitamin C and E and 0 mg/kg vitamin C and E(control) , respectively. The highest and the lowest Feed Conversion Ratio (FCR) were measured in control and diet 50 mg/kg vitamin C and E. There is a significant difference in their treatments (P<0.05). Also, the lowest and highest amount of Weight Gain (WG) were observed in (E) treatment with 165.04% and 117.5% in control, the highest and lowest Specific Growth Rate (SGR), Protein Efficiency Ratio (PER), Condition Factor (CF) was found in control and treatment 50 mg/kg vitamin C and E, respectively(P<0.05). In conclusion vitamin C and E have an important role in enhancement of growth performance and feed efficiency of rainbow trout.The highest red blood cells were found in combined treatments and which the vitamin C was added.The highest RBC were found in E treatment(1.1×104 /mm3) and the lowest one in control (P˂0.05). Counting white blood cells also confirmed highest quantity in combined treatments with (69.83×104/mm3) and the lowest one (28.83×104 /mm3) in control. In conclusion these vitamins have a significant role in blood characteristics. Meantime, the resistance against termal stress was measured at the end of 60 days by facing fishes into 5 centigrade warmer water so consentration of Cortisol and Glucose measured for this reason.The lowest cortisol amount was measured in E treatment with 188.74 ng/ml and the highest was found in control(P<0.05). There was a significant difference in blood glucose consentration of fishes in F treatment with (78.66 mg/dl) and control with 136 mg/dl as a highest one(P<0.05).

The influence of Mozart's sonata K.448 on visual attention: An ERPs study

In the present study, the effects of Mozart's sonata K.448 on voluntary and involuntary attention were investigated by recording and analyzing behavioral and event-related potentials (ERPs) data in a three-stimulus visual oddball task. P3a (related to inv

Supply of Astaxanthin and its combinations through live feed (Moina micrura) enrichment affects the growth, survival and fatty acid profile of Macrobrachium rosenbergii larvae

A study was carried out with three replicates to determine the effects of feeding Moina micrura enriched with astaxanthin alone (M1) or astaxanthin in combination with either vitamin E (M2), vitamin D (M3) or Cod Liver oil (M4) on the growth, survival and fatty acid composition of giant fresh water prawn Macrobrachium rosenbergii (de Man) larvae. Growth rate was expressed as the time taken to the settlement of 95% post larvae. Maximum growth, the lowest time taken to the 95% PL settlement (38.5±0.50 days), was observed in larvae fed with M3 Moina. The highest survival rate (66.0±1.00%) was observed in those fed with M4 Moina and the second highest survival (61.0±1.00%) and growth rates (40.0±0.00 days) were shown with M2 Moina. The minimum values for both growth (42.5±0.50 days) and survival (33.0±1.50%) were observed in the group fed un-enriched Moina. Results also showed that the survival of prawn larvae increased as the quantities of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) increased in the dietary Moina. The highest levels of EPA (5.57±0.21%), DHA (3.50±0.21%) and highest total Highly Unsaturated Fatty Acids (HUFA) (13.87±0.68%) were seen in the Moina fed on astaxanthin and Cod Liver Oil (CLO). The results of the study showed that the nutritive quality of Moina, with respect to important fatty acids, can be increased by enrichment and will influence the growth, survival and the fatty acid composition of fresh water prawn larvae fed on them.